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Author Archives: Susan Zheng

The end of BSURF is only the beginning…

It’s the end of the program, but only the beginning of my scientific journey. In the past 8 weeks, I have made my first steps into research – and it’s been an amazing experience. During what felt like such a short amount of time, I’ve grown so much as a scientist.

Before the program, I didn’t have an accurate picture of what research was like. Now, I’ve gained a deeper understanding of and appreciation for science. From learning how to clone DNA to understanding the mechanisms of heart regeneration, from reading scientific papers to experiencing the lab environment, from working on an independent project to hearing Duke faculty share about their research, I have a much better understanding of what a scientific career is like. It is something I definitely want to continue to explore.

I’ve learned that science is a lot about asking questions. Whether it’s asking why is this important, what does this do, or how does this work, the root of science is asking questions and striving to find answers. Science is intricate and tricky in this sense. Sometimes, there’s no clear-cut answer. And when there is an answer, it doesn’t always come right away. It’s the scientist’s job to try to crack open this mysterious puzzle, a difficult but highly rewarding task. I think this is what makes science all worth it. You are able to discover things that no one knows, test your curiosities, and contribute to society in meaningful ways.

Walking in to lab on the first day, I’ll admit I was nervous. Here I was, in this room full of expensive, fancy machines I didn’t know how to work and intelligent scientists performing complicated procedures I didn’t understand. Now, however, I’ve gained a sense of confidence and comfort. Don’t get me wrong, there’s still a ton of things I don’t know how to do. I’m still learning. But, every day, I came into lab, excited to work on my project. And every day, I walked away learning something new. Whether it was the new skills I’ve learned, knowledge I’ve gained, or relationships I’ve developed, this experience has been truly valuable. It’s the last day now, and I’m no longer nervous. Instead, I’m more curious, eager, and excited to continue to discover.

I am so grateful to Dr. Grunwald and Jason for all their hard work to make this experience possible for us. I also want to thank the entire Poss lab, especially my PI and my mentor, for giving me the opportunity to work with them and for all their patience, support, and guidance. This experience has truly been a great gateway into research and I can’t wait to see what the future holds for me.

Faculty Seminar Reflection: Dr. Raphael Valdivia

Every week this summer, we had the opportunity to listen to esteemed Duke scientists share about their current research as well as their career paths. Not only was it fascinating to learn about the diverse projects they’ve dedicated their careers to, but it was very intimate to learn about their personal journeys that have made them the successful scientists they are today. It was comforting to hear their stories and to learn that a lot of them did not follow a linear path to become a scientist. Instead, many of them took non-traditional paths that allowed them to explore many different avenues and discover many valuable experiences.

Though I enjoyed listening to all the faculty seminars, Dr. Raphael Valdivia’s talk caught my attention the most. Dr. Valdivia’s lab studies the pathogen chlamydia, a bacteria that is responsible for infecting thousands of men and women with sexually transmitted diseases as well as causing infectious blindness in humans. His work mainly involves identifying the mechanisms of how chlamydia mediates reprogramming of host cells and investigating how they occur. One of the mechanisms he observed is how chlamydia infections prevent cells from undergoing apoptosis, or cell death. I thought it was interesting how chlamydia uses this mechanism to proliferate in cells.

However, what really drew my attention was how personal he was with sharing his story. Dr. Valdivia grew up in Lima, Peru. He obtained his undergraduate education at Cornell University, attended Stanford for his Ph.D. and Berkeley for his post-doc. It wasn’t an easy road for him, however. After not being able to get into grad school right after college, it was his amount of determination to get in after his gap year that really inspired me. He was also faced with many tough decisions, such as having to decide to work in a lab where he values intellectual stimulation and being outdoors where he loves the adrenaline rush of climbing mountains. His gap year helped him decide that his true calling was with science and discovery. He really emphasized how important hard work is. If you’re willing to put in the work that it takes, you can go far. Ultimately, it was this amount of determination and authentic passion he has for his work that I enjoyed hearing the most.

Journey to Create RNA Probes

Over the past 6 weeks, I’ve learned a lot. I’ve learned how to pipette the optimal mix of reagents to amplify DNA for PCR. I’ve learned how to clone DNA with a plasmid vector. I’ve learned how to perform ventricle resection on zebrafish. And most importantly, I’ve gained a better idea of what research really is.

Research definitely takes time. Whether it was waiting a day for my bacteria inoculation or 6 hours for my RNA transcription, research actually involves lots of just waiting time. Thankfully, I was able to turn the waiting time into productive work by performing other steps or reading papers. Research also involves a lot of repetition. I can’t even count how many times I ran a PCR and gel this summer. It involves lots of repetition in the sense that repeating procedures makes you better at it. But also, troubleshooting. Sometimes, a step may not work and you may be stuck on one part for a long time, trying to figure out what’s wrong. This involves repeating the step many times and changing one thing at a time, trying all possibilities to get it perfect. I actually enjoyed this troubleshooting process because it was like solving a puzzle to me.

The main part of my project was to create 4 RNA probes for my genes of interest to perform in situ hybridization. This may sound simple to do, just create four strands of complementary RNA. Easy, right? The reality was far from this expected simplicity. In fact, it took over 6 weeks to create three probes (one gene refuses to be amplified and after tons of troubleshooting will still not work) that I just finished this Tuesday. This is really exciting for me because I can finally run all my in situs with my probes. This week will be the week of results where I can see where in whole 3 days post fertilization embryos, 6 week zebrafish hearts, uninjured zebrafish adult hearts, and injured zebrafish adult hearts the genes are being expressed. I cant wait to complete these final stages for my in situs.

In the journey to create RNA probes, I got to learn and experience a multitude of diverse procedures and techniques. Now, I feel much more comfortable performing these protocols and feel that I have a comprehensive overview of the common protocols related to zebrafish. Just the journey and learning everything both conceptually and mechanically have been so rewarding. At this point, results don’t even matter. This experience has been much more valuable (of course, it wouldn’t hurt to get some results).

Slowly by surely, I’ve been putting all the little puzzle pieces together. The past 6 weeks have gone by so quickly, I can hardly believe that the program is drawing near its end. It’s been such an enriching and thrilling experience working in the Poss Lab and I can’t wait to see the final results of my project!

Chalk Talk Reflection

I really enjoyed hearing everyone give their chalk talks this week and learning just how diverse all of our projects are. From plant germination to genetic mutations, gene expression to viruses, each one of us is taking part in really interesting science.

One of the chalk talks that especially caught my attention was Ricardo’s talk about brain-machine interfaces (BMI). A brain-machine interface is a communication pathway between the brain and a system of devices composed of a decoder that interprets electrical signaling in the brain, a machine that translates signals into movement, and sensory feedback that relays signals back to the brain. This network of brain and machine communication allows for the development of brain controlled prosthetic limbs to be used by patients who suffer from paralysis such as quadriplegia. His project focuses on studying the relationship between lag times (the delay between neural firing and actual movement) and unimanual vs. bimanual actions in monkey models. This is done by comparing velocity models and neural firing models for each type of action. Studying lag times can help improve the efficiency of brain-machine interfaces by making movement feel more natural for humans.

What amazes me the most about his project are the possible applications brain-machine interfaces have for human health. The use of BMI in patients who suffer from limb paralysis would allow them to move again, essentially changing their lives. I really found learning about the engineering side of research to be interesting because of this direct application for humans.

I look forward to hearing about his findings as well as all my fellow BSURF researchers’ findings!

Life in Lab

No day is the same working in the Poss Lab. Every day consists of new experiments to carry out, new things to learn, and new questions to ask.

The first two weeks of lab largely consisted of shadowing my secondary mentor and learning basic protocols. After gaining experience and comfort in conducting PCR reactions, gel electrophoresis, heart extractions, embryo injections, cloning, and cryosectioning (just to name a few), I have really enjoyed gaining independence in my work.

My project looks at 4 different genes and they’re currently all at different stages in my experiments. I have already ran in situ hybridization for one gene while I’m still at step 1 in trying to isolate the segment of DNA I want for another gene. Thus, every day I continue working on my project, whether it’s the final step for transcribing my RNA probe or trying to troubleshoot a PCR for the tenth time. My days will usually involve running multiple PCR reactions and gels. If my gel runs properly, I can extract the DNA band and purify it. Then, I clone the DNA into a plasmid and transform it in bacteria. After the bacteria grows, I set up a colony PCR to verify the DNA segment and send the plasmid for DNA sequencing. If the DNA sequence looks good, I inoculate more bacteria, extract the DNA with a midi-prep, linearize the plasmid, purify, and transcribe (if all goes smoothly) with many PCR’s and gels in between in order to double check each step. Usually, a step won’t work correctly and I have to try to figure out what the problem is. Troubleshooting a step may take days, so I repeat a step many times, changing one thing at a time in order to determine what is wrong.

Depending on the day, I may also practice embryo injections which involve setting up zebrafish matings the day before. I collect the embryos, prepare the injection mixture, and inject hundreds of zebrafish embryos. I may also need to make cDNA for my PCR which involves dissecting zebrafish hearts, extracting the RNA, and reverse transcribing it to make cDNA. I am usually conducting many experiments at once. While I’m waiting for my PCR, I may be embedding hearts in tissue freezing medium or screening zebrafish for mutants. Some days I am busy going back and forth from the lab to the fish room. While other days I have more waiting time when I can read some papers.

My routine largely consists of the same protocols, but working every day in lab is still exciting. Although creating an RNA probe for each gene involves the same steps, each gene behaves differently. Thus, applying each step to each gene creates different results, so I never know what I’m going to get. Sometimes, things don’t work out so it can be challenging but I enjoy trying to solve the puzzle. I have definitely learned that science takes time but I am excited to discover what it will reveal.

Zebrafish Cardiac Development and Gene Expression

The Poss lab focuses on the concepts and mechanisms of regeneration in zebrafish. Zebrafish are a known animal model system for studying regeneration because of their remarkable ability to regenerate lost tissue after injury. Adult zebrafish are capable of regenerating tissue in the heart, as well as fin, spinal cord, and retina.dsds

Poss, Kenneth D. “Getting to the Heart of Regeneration in Zebrafish.” Seminars in Cell & Developmental Biology 18.1 (2007): 36-45.

In fact, it has been shown that zebrafish are able to regenerate their hearts, not due to stem cells, but the proliferation of spared cardiomyocytes (Poss, Wilson, & Keating 2002).

Poss, Kenneth D., Lindsay G. Wilson, and Mark T. Keating. “Heart Regeneration in Zebrafish.” Science 298.5601 (2002): 2188-190. 1 July 2016.

This picture shows the regeneration of ventricular myocardium in the resected zebrafish heart. After 20% of the ventricle was amputated off, by day 60, no sign of injury remained and the heart is fully repaired.

In contrast, mammals do not have this capacity to regenerate and repair its tissue efficiently.Untitleddsd

Poss, Kenneth D. “Getting to the Heart of Regeneration in Zebrafish.” Seminars in Cell & Developmental Biology 18.1 (2007): 36-45.

Thus, injury in the heart often leads to scarring and fibrosis. This makes diseases such as heart disease a significant threat to human health. Studying the mechanisms of regeneration in zebrafish can illuminate factors that may be applicable to the stimulation of human heart regeneration.

My research project focuses on heart development. Specifically, I am looking at 4 different genes and the roles they play in cardiac development and regeneration. The zebrafish heart is divided into two main parts – the atrium and the ventricle. The overall concept of this project is to examine what factors in heart development are responsible in pushing some cells into differentiating into the atrium while pushing other cells into becoming the ventricle. In order to examine these genes’ influence in heart development, I look at whether these 4 genes are atrial specific, meaning they are uniquely expressed in the atrium, or whether there are ventricle specific, meaning they are only present in the ventricle. Two of the genes I am examining were hypothesized to be atrial specific while the other two genes were hypothesized to be ventricle specific.

In order to examine where in the heart these genes are being expressed, I am performing in situ hybridization with adult zebrafish hearts. In situ hybridization is a method that uses a labeled complementary piece of DNA or RNA to locate a segment of DNA or RNA of the gene of interest in tissue (Wikipedia). I will first make a RNA probe that is a segment of RNA complementary to one of the genes of interest. This RNA probe will contain digoxygenin tags that will then be used to immunohistochemically label cells expressing the complementary mRNA. The areas of the heart where the gene is being actively expressed will turn blue. With this method, I will be able to see exactly where in the heart the genes of interest are being expressed. I will also perform in situ hybridization on 6 week old zebrafish to see where the genes are active during the earlier stages of zebrafish life. Furthermore, I will perform in situ hybridization on injured adult zebrafish hearts in order to see where in the heart these genes are being expressed during regeneration and if there are any changes in levels of expression compared to uninjured adult zebrafish hearts. The next step in this project would be to identify possible atrial or ventricle specific enhancers of these genes, clone them in front of a GFP gene, inject into zebrafish embryos, and observe where in the heart these enhancers are active (turns green). Then, CRISPR/Cas9 gene editing would be used to delete the enhancers of these genes and to examine the effects of the deletion on the cardiac development of zebrafish. Overall, with this project I am hoping to find out what roles these four specific genes play in heart development and regeneration.

Unlike the majority of the Poss Lab that focuses on regeneration, my project looks more at heart development. However, development and regeneration are closely linked, so I hope I will be able to contribute information on the roles of these genes in the heart in terms of both cardiac development and regeneration.

Interview with Dr. Ken Poss: Discovery, Exploration, and Fearlessness

Ever since he was a kid, Principle Investigator, Dr. Ken Poss, has always had a love for biology and animals. Dr. Poss began his scientific career at Carleton College, a small school in Minnesota, where he studied biology. The first time he experienced research was his junior year, when he worked in a biochemistry lab. His school fostered a highly interactive atmosphere, such as extremely small classes. In fact, he was the only person in his lab besides his mentor! In 1992, he furthered his studies and attended MIT to pursue his Ph.D. in biology. At the time, he was very interested in new technology in mutant mice. There, he worked in Rudolf Jaenisch’s lab where he experimented with mouse technology and cloning. He then joined Nobel Prize winner Susumu Tonegawa’s lab where he studied the effects of a particular enzyme on learning and memory in mice. He thoroughly enjoyed his time at this lab because he was really able to gain a sense of independence in research. After his Ph.D., Dr. Poss switched fields when he started on his postdoc in Mark Keating’s lab at the Children’s Hospital in Boston. He wanted to be able to apply genetics in a field where he could do independently. At the time, regeneration was a highly unstudied field of work. Thus, he started working with zebrafish and regeneration and became one of the first people to apply molecular biology to regeneration. In 2003, Dr. Poss came to Duke where he became a member of the Department of Cell Biology and was named a James B. Duke professor. He continues to study the mechanisms of heart and tissue regeneration in zebrafish.

Dr. Poss’s favorite part of what he does is being able to be a part of the larger scientific community. With so many fields that he is a part of – zebrafish, heart development, regeneration, embryonic development- he is able to connect with all kinds of people. He enjoys being a part of this wide spread international community of fellow talented scientists.

As the P.I. of his large lab, Dr. Poss no longer conducts experiments but instead manages his lab. However, he loves seeing people in his lab get results. To him, it is just as exciting as getting results himself. His current goal, besides learning more about the mechanisms of regeneration, is to see people from his lab succeed and make discoveries. He enjoys being a part of trainees’ development and to see them grow as scientists.

Key advice Dr. Poss would give to people thinking of going down the path of scientific research is to make smart, key decisions. Research isn’t always about being the smartest or knowing a field inside and out. It is more about being able to embody discovery, exploration, and fearlessness. It is important to view the “unexpected” in a positive way. He thinks that unexpected results are often the most fun, the most important, and the most rewarding. Through these unexpected results, one can contribute to the field and make a big impact.

Thank you Dr. Poss for taking the time to talk to me. Listening to your scientific journey has widened my perspectives on research and has allowed me to reflect on my own path in terms of discovery, exploration, and fearlessness.

Work, Think, and Act like a Scientist

A lot of words characterized my first week here:

  • I was excited to begin my first real research experience.
  • I was nervous that I would make tons of mistakes.
  • I was appreciative for everything everyone has done so far – Dr. Grunwald for making this program possible, Jason for supporting us and giving advice, Duke professors for sharing their research experiences, my secondary mentor Matt for guiding and teaching me, and the Poss Lab for letting me work with them this summer!

Conducting real research is a lot different from learning science in a classroom. When I work in the Poss Lab, I’m not conducting experiments everyone has done before in their organic chemistry classes. Real research is open-ended. There are no clear, numbered steps and I never know what I’m going to get. Thus, over the summer, I hope to be able to work, think, and act like a scientist. What does this mean?

I hope to work like a scientist. I hope to be able to understand what real research is like and to gain a deeper understanding of the scientific process at play. I already feel like I have learned a great deal this week. Whether it was learning new lab techniques (I did my first PCR this week…and my second…third…I lost count) as well as understanding the fundamental concepts behind regeneration (what the Poss Lab focuses on), I hope to not just follow protocols and to transfer colorless liquids with a pipette, but to truly understand what is going on. I also expect to make mistakes, in fact I already have. I don’t expect all my experiments to be successful- whether it was my DNA not running properly on gel or my bacterial transformation failing. What’s important, however, are the valuable takeaways in learning from my mistakes.

I also hope to think like a scientist. I hope to always be constantly asking questions (thank you to Matt for patiently answering the endless amount of questions I had) and to be learning something new every day.

Lastly, I hope to act like a scientist. I am excited to continue to build upon my genuine interest for biology. Not only will I be doing what I enjoy every day, I will also be delving deeper into regeneration research as I hope to contribute as a member of a team of researchers at the Poss Lab as well as build long lasting connections with these talented scientists.

This first week has already been everything and more and I look forward to explore what’s to come in the next 7 weeks.


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Underqualified Teachers

Underqualified teachers are defined as those not meeting state licensure standards and subject matter expertise. In measuring a teacher's quality, degrees and certification are used as the primary indicators of credentials. Underqualified teachers are in the majority in poor and minority school districts. Students of underqualified teachers perform worse than those with highly qualified teachers. Schools with a high number of underqualified teachers are more likely to be identified as low performing. Due to teacher shortages in high school mathematics and science, and all grade levels in special education, underqualifed teachers are assigned to these teaching fields. A 2009 report from the U.S. Department of Education on teacher quality indicates that approximately one-third of teachers on qualification waivers were assigned to high-poverty schools. Educational reformers ...

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Resistance Theory

Resistance theory promotes a politicized reading of the actions taken by students to oppose power hierarchies that reinforce systemic inequity related to class, gender, race, and sexuality through the imposition of curricula, rules, and culture in schools. Grounded in Marxism, postmodernism, feminism, and poststructuralism, resistance theorists provide perspective for the actions students take to complicate the political and social order of society that is replicated in schools. Resistance theorists argue that the moral and political acts of resistance that students engage in are too often misinterpreted and explained simply as misbehaving. Students and the groups they belong to exert agency in multiple ways with the ultimate objective of transforming the policies and institutions that create and maintain privilege for some while marginalizing others.

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Who Dey Breet

What does who dey breet mean?

Who is breathing?

Who dey breet is another slang phrase coined by Nigerian-American music artist Davido, of e choke fame. Translated literally, "who dey breet" means "Who is breathing?" It is used to call attention to a breathtaking moment, by suggesting that everyone should be so awestruck that they are unable to breathe.

Where did who dey breet originate?

Davido first used who dey breet in response to seeing fellow Nigerian artists Wizkid and Burnay Boy win awards at the 2021 Grammys. Overcome by emotion, Davido tweeted "Who Dey breeeeeeeeeeeet." The artist's fans then began using variations of who dey breet.

How is who dey breet used?

Anytime you encounter a moment, picture, or statement that is so powerful it takes your breath away, you can respond with who dey breet. You can also use who dey breet in your own social media posts, when you think a post is stunning enough to leave people breathless.

"Omo e dey dem sure, e hit jackpo, who dey breet"
Davido's original "who dey breet" tweet

Davido's original "who dey breet" tweet

Related Slang

Last Updated: June 3, 2021

Who dey breet definition

This page explains what the slang term "Who dey breet" means. The definition, example, and related terms listed above have been written and compiled by the Slangit team.

We are constantly updating our database with new slang terms, acronyms, and abbreviations. If you would like to suggest a term or an update to an existing one, please let us know!


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Narcos Slot Overview

“Narcos” is based on the true events that happened to Pablo Escobar, an infamous drug lord from Colombia in the 80s. The first two seasons look at Pablo Escobar, while season three looks at other members of the drug world in the late 80s, and there is a possibility that season four will continue down this path. Season four is said to be released at the end of 2018, so some of the best online casino slots reviews say that the game could be out by then, in time for the season to start. These predictions could come true or they could not.
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You could say that NetEnt’s Narcos slot machine will focus more on the story from seasons one and two, with supporting characters being high-value symbols. These symbols could be Steve Murphy and Javier Pena who are the detectives tasked with the job of finding and catching Pablo Escobar. The other main characters could make up the rest of the high-value list. But there may only be five characters to have on the slots which seem to be the standard.

NARCOS Slot Screenshot

What If

  • The low-value icons may not be the typical A, K, Q, J, and ten like in most online real money casino games, but motifs from the series such as bags of cocaine, cars, televisions, or maybe even soccer ball. Items that can link to Pablo Escobar to offer more variety for the design of the slots.
  • Since the series is complex, what if instead of the usual five reels with three rows, it could be five reels with four rows to add more suspense. The pay lines could go up to 30, but in unusual formations, so it is not for the weak of the gut.
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  • “Narcos” is a dark and gritty series, and the design of the slots should reflect that style with the background being that Colombia at night in the 80s. The slots can be a bit messy, but the symbols will be clear and stand out against the dark.
  • Pablo Escobar can be the wild symbols which can replace the other symbols as well as double the winnings because he is the boss.
  • Bonus rounds could be different games such as multiple-choice trivia that can increase your winnings. So you could get a reward for watching the show.
  • When the high-value characters win a pay line, they could say a quote for the show.
  • The music would be similar to the series with its Colombian influences, and the sound of the reels spinning can be the sound of cars driving.
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Narcos Slot Conclusion

These are merely predictions and guesses. We will have to see what NetEnt brings with its Narcos slot. Escobar started as a thief who went after Electronics and Televisions in the 70s and then came in control of 80 percent of the cocaine that was being shipped to the USA. There is also a storyline that he supported charities and soccer clubs.

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That Time We Celebrated - Original Art

SKU: SG-AC5-01

"That Time We Celebrated", 8x8 inches. Unframed.

This solid square painting on wood features two branches in a purple vase set on a striped tablecloth. The vibrant rose pink, cobalt, and magenta colors play off each other and will make feel more cheerful.

Mother's Day Special

To celebrate Mother's Day this collection comes with a FREE, specially made Mother's Day card, sticker and wrapping paper. After you've placed your order we'll get back to you in 1-2 business days to confirm your personalizations.

Not buying this for mom? Also great! You can totally gift this to yourself as well because you are awesome :) You'll receive a different FREE greeting card as a gift instead.

Painting Details

  • 8x8 inches, with the 1/2 inch profile painted and continued along the edges
  • Signed and dated on the back
  • High gloss, professional varnish for maximum protection
  • Acrylic & acryla gouache painted on wood

How to buy

1. Add this painting to your cart. Your gift set will be included, no need to do anything extra.

2. Select the $7 flat fee shipping rate (worldwide) at checkout and purchase

3. When it arrives, use the FREE mother's day gift set (see above) and enjoy gifting.

Flat fee shipping (worldwide)

A flat fee worldwide shipping fee of $7 will be offered at checkout. You won't need to add a code, it's automatically available.

Possible customs fees are not included, please check if this applies to your situation before purchasing. Please allow up to 5 business days for shipping (if you need it sooner, please email us). Thank you!

Please note that we cannot ship to some countries because of COVID shipping restriction implemented by the Canadian postal service. This is outside our control and can change on a daily basis. If this happens we will contact you for an alternative shipping address or refund you in full.

A note on colors... The colors may differ slightly because all screens are different and have a different level of brightness.


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A Powerful Lesson About Privilege SCORE 160
Words. SCORE 229
Fat, loud, arrogant and conservative? SCORE 186
They never really stop. SCORE 181
A Rainbow cricket. SCORE 119
Two bears in a serious meeting SCORE 83
Jim Carry, in a nutshell. SCORE 29
Boring college class bingo. SCORE 162
United States Of Autocomplete SCORE 103
Dobby is a free elf! SCORE 140
Why we love Jennifer Lawrence. SCORE 304
Every great scientific truth. SCORE 163
Let them come... ðŸ˜¼ SCORE 25
Star-crossed lovers. SCORE 163
Lieutenant Crunch SCORE 94
Rise and Shine !! SCORE 22
Niceness Pros SCORE 196
Words. SCORE 281
Thanks Mom SCORE 164
Air rings. SCORE 174
Our professor wears his hat and cape every time he conducts an experiment SCORE 99
Got Rejected By Many Tv Shows Dues To His... SCORE 133
Apparently my doctor always opens the door with his pen in hand SCORE 81
Service dog at a high school got his picture taken for the yearbook SCORE 71
I have a dream... SCORE 165
It's just a hoax, bros. SCORE 24
Sharing is caring SCORE 28
When nerds get married. SCORE 284
Don't give in! SCORE 85
Weather in Texas. SCORE 170
An African Bush Viper - Pretty but Venomous SCORE 119
Slow Robots do love too!
What are brothers for? SCORE 158
Justin Flursery SCORE 80
She will literally rest her head on anything SCORE 79
Too soon? SCORE 50
Toxic family situation. SCORE 83
And it's only January SCORE 54
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Getting drunk in Canada. SCORE 197
Kitty! SCORE 155
Lockpick earrings SCORE 142
The girl with the digital camera. SCORE 218
Gonna need a restore disk for the restore disc... SCORE 38
Lucky to be there, though. SCORE 25
I'm so very fawned of you. SCORE 132
Vegan Ice cream SCORE 65
Dog moves bed to sun SCORE 135
Well at least we know now... SCORE 14
Mistakes were made. SCORE 30
The struggle is real SCORE 179
Do the playlist shuffle! SCORE 21
haven't you heard? SCORE 153
When you hold a shell to your ear. SCORE 105
Sage advice, weirdo. SCORE 99
Someone's ready for his morning walk SCORE 186
I'm not cute, I'm a mighty dinosaur SCORE 67
Baroque Obama. SCORE 132
Cat Vision SCORE 75
Zero to one hundred, really quick, Janet. SCORE 16
Homework is more important than health! SCORE 44
Messing with Drunk People SCORE 123
Welcome to Wendy SCORE 81
There's no sabbatical from being smooth. SCORE 197
How I feel any time I order dessert at a restaurant. SCORE 137
Like a boss. SCORE 161
The positive side of not getting vaccinated. SCORE 214
Thanks Siri. SCORE 103
A moment of silence for ironayra to reminisce over his noble sacrifice. SCORE 178
They say if you love your job... SCORE 93
Barack Obama at Harvard University, where he served as editor of the Harvard Law Review (1990) SCORE 72
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Shut Up, Taco SCORE 102
The tables have been turned. SCORE 347
peter parker is dumb SCORE 48
Life of an introvert SCORE 166
The gravy we deserved. SCORE 16
Whenever I pee in the pool. SCORE 96
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Friday, June 23, 2006

Book Reviews: Ladies #1 Detective Series

June 23, 2006

I have finished three more books in The Ladies' No. 1 Detective Series by Alexander McCall Smith, and I have to say I just love these books. I asked a friend of mine recently if she'd read these, and she said she'd read the first one and it was "Cute." Wow. That had to have been a surface-level read, because this series is so far from being "cute." There is wonderful language in each novel, and this beautiful river of tradition flows through every chapter. To only read this as a plot book--a fun summer beach novel--is a shame. So much is missed by skimming. Mma Ramotswe is a wonderful character, and Botswana comes to life under McCall's Smith poetic writing. Read it as "cute" if you wish, or read deeper and be mesmerized by the theme of people, family, and country.

Saturday, June 10, 2006

Book Review: Brave Enemies

June 10, 2006

I loved this southern novel by Robert Morgan, also the author of Gap Creek, another of my favorites. This is a fascinating story of a young mountain woman during the Revolutionary War who, after a series of extremely unfortunate events, decides that her best chance for survival is to pretend she’s a boy. What happens in the hills between the rebels and the loyalists and those suspected of being a spy or seditionist on either side is pitiful and shocking. Ultimately Josie unintentionally joins the militia and fights at the fateful battle at Cowpens in January 1781. This is a fast-paced read. I’m glad to know Robert Morgan has several other novels I’ve not yet read!

Friday, June 9, 2006

Book Review: Monster

June 9, 2006

Frank Peretti's Monster is a good summer read. I remember Peretti's other novels as being much more visual and spiritually impacting. This novel is just a satisfying "Sasquatch" read, for those of us who have been fascinated with Sasquatch since watching the Six Million Dollar Man face off with the critter. This book goes on my list as a good plot read, but the writing itself is lacking and the dialogue is stilted. He also got quite preachy at several points; in fact, my eyes started glazing over at times. That said, if you've always had an interest in Bigfoot, this is a fun read.


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Thursday, May 19, 2016

Books Read in April

One Plus One by JoJo Moyes
The story: Jess is a single mom raising a brilliant, quirky daughter as well as her ex-husband's teenage son. She works hard as a housecleaner/bartender and barely has enough money to feed the kids, much less send her daughter to the exclusive private school that has offered her a nearly full scholarship for her math skills. And then she crosses paths with Ed, a multimillionaire who is about to lose everything. The four of them accidentally embark on a journey to get to the Math Olympiad and end up becoming a family.
Me: This was a fun book. It's a beach read. Nothing terribly amazing, just fun and happy for the most part. Great for summer!

The Nightingale by Kristin Hannah.
The story: French sisters Vianne and Isabelle, daughters raised by an emotionally cold father, have approached life completely differently. Vianne is careful, comfortable in her marriage, but fearful; Isabelle, 10 years younger, is impetuous, rebellious, and reckless. When the Nazis invade France, they must both make impossible choices. Vianne's husband is drafted, and she and her young daughter must survive without food or money—and with a German captain living with them. Isabelle joins the Resistance and risks her life daily to save others.
Me: I loved this epic tale of survival during World War II. Isabelle gets more attention; her choices are flashier and more dramatic. But Vianne is the character I can identify with more: the one who does anything she can to provide for her family, the one who doesn't know how strong she is until she is forced to protect those she loves— the one who sometimes makes bad decisions because she doesn't understand how cruel people can be. I am fascinated with WWII stories, largely because there are so many aspects of the war to explore: soldiers on all sides, families and individuals in all kinds of circumstances, the home front, the Holocaust, the Japanese-American experience, the unsung heroes, post-war, etc. And while I've read similar stories, The Nightingale takes a fresh look at how two small people can make a huge difference in a war that encompassed the whole world. Highly recommended.

World War Z: An Oral History of the Zombie War by Max Brooks.
The story: The Zombie Wars are over—what really happened? This is the history of the Zombie Wars as told by various key players as well as regular people—all survivors of the unthinkable. Around the world, various governments, families, and individuals handled the zombie attacks in different ways. These are their stories of survival, pieced together in interview form.
Me: Meh. I found the interview style of the book to be tedious and confusing. I found myself constantly turning back a page or two, wondering whose POV I was currently reading. I should say that I love zombie movies and shows; I just didn't enjoy the style of this book. I would have loved reading a regular novel chronicling the events, but this left me mostly just feeling meh

To Kill a Mockingbird by Harper Lee.
Multiple re-read of my favorite novel. With only one exception, this gets the "book of the year" award from my literature class.
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Our Town by Thornton Wilder.
Re-read of a wonderful play. My high school students absolutely loved this play! Although it's a portrayal of small-town life in the early 1900s, my kids found it to be both universal and contemporary. Highly recommended reading, even if you've seen the play.


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text:
 Branch: George Washington Smith b 1785 VA m Mary Tyner 2 David 3 David 4 David: Smith DNA Official Project - All Locations

Smith Official DNA & One Name Study

Smith Official DNA Project at FamilyTreeDNA

Branch name aBL5vU18060458
Description George Washington Smith b 1785 VA m Mary Tyner 2 David 3 David 4 David
DNA Test Kit#/ Type 3659**** Vendor: FTD YDNA
YDNA Haplo: I-M253 YSNP:I-FTA8201 mtDNA haplo:
DNA Matched Group GRP-I-M253-33 - Click to see all members in this group
BigY Smith Group BY-I-M253-3 - Click to see all members in this group
Autosomal Matched Group GRP-AU-15 - Click to see all members in this group
People in Branch Individuals Families
Trees (Top Ancestor/Pedigree)
Contacts JaneBonny
To contact JaneBonny, you must be registered and logged on. come register on smithsworldwide.org so you can be contacted
Discuss Come Discuss This Group GRP-I-M253-33 on the Smiths DNA Forum
Come Join the Discussion at Smith DNA Facebook group
Leave a query on the forum about George Washington Smith b 1785 VA m Mary Tyner 2 David 3 David 4 David  Or contact admins

More statistics Smith Matched Groups  Smith Researchers List Smith/Schmidt Discussion Forum  YDNA results by group on FTDNA

Contact indicates a person has supplied his/her lineage for the project. If no branch contact info@smithsworldwide.org or if this is you, register on the site so you can be assigned to this branch. Registered members can be contacted via private message/email.

Contacting a participant. Use the contact information, above. if the member is registered on this site, you may send a private message.  You will also need to be registered and logged on. You can also go to a given ancestor page, and post a query to the forum which will also post to that ancestor's page. Post a question regarding the line on the Smiths DNA Facebook group. If on FamilyTreeDNA, look up the match list and click on the email icon for a given matching kit. Post a comment on the guestbook.

*What is a Branch? Each member with a particular line of descent is represented by a branch. Each branch has a unique branch ID, and a description, and, if the person has registered, will have a member name tied to the branch. The branch line is included in most reports, and shows whether the branch represents YDNA, mtDNA, autosomal, or is tree only.
On the Smithsworldwide trees, there are several ways to determine the status of a DNA test. If it's simply a listed line that NO ONE has claimed, you will see, in the Branches/DNA section on the ancestor person page, "No DNA tester yet or not listed branch". Means we are waiting for someone to come along whose line this is to claim it and hopefully do a DNA test. If you see Smith Branch(YDNA, FF, or mtDNA) means there is a DNA kit for that person of the type specified (ie, YDNA, Family Finder/Autosomal or mtDNA). Click on the link to contact that person, see the pedigree line, go to a group link(if there is a matched group for that kit). If this is someone's line that has not done a DNA test but has supplied a tree, you will see a branch listing and Tree-No DNA.


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Archived Opinion

Newspapers with real reporters and editors matter

Newspapers with real reporters and editors matter

By Frank Queen • Guest Columnist

I was surrounded by newspapers growing up. Dad worked for the government in the 1960s and we lived in Alexandria, a suburb of D.C. Every day we had five newspapers delivered to the house.

Dad started reading when he got home and only stopped to eat supper. You could try to talk to him when he was reading, but he didn’t hear you unless you could get him to lower the paper. If you wanted to hang around with him, you might as well sit down and pick up a paper yourself.

By reading all these papers, all of us in the family learned to detect the slants of the different papers. The Washington Post and Washington Star were pretty consistent; the Wall Street Journal ignored Washington unless there was a dollar sign in the story; the Raleigh, Alexandria and Waynesville papers ignored Washington news completely.

“News” was defined by what the papers covered: if they covered the story, it was news; if they didn’t, it wasn’t.  There were reporters and editors in every paper making the decisions. The papers competed with each other, both for new stories and new details on old stories.

Washington was filled with people in government who wanted to get things in the papers, to promote their issues and themselves. “Gossip” columnists at the papers (Drew Pearson at the Washington Post was the most famous) made it their business to publish embarrassing stories every day.

Related Items

All of this history reads now like — well — history.

News comes to us now as a firehose of information, we are told. Some folks put “news” in quotations. Newspapers, and editors, we are told, are obsolete, since facts are what the speaker says they are. Lies, referred to as “alternate facts” by the liars, are not apologized for.

Well, not so fast.

My need for context, and editing, and questioning is more important now than ever. So long as the editors and the reporters take their jobs seriously then they have me — and an audience — reading them. I don’t need someone to think for me: I need someone to ask questions for me and to remember what this guy said back when he was running for office, as well as what he is saying now that he’s in. It is useful for me to read the words of a reporter who knows her beat and who reports not just the words, but the look on the speaker’s face and what the listeners said too.

The value of a newspaper (or magazine or scripted media) is not in getting the simple facts — the value comes when the writer and editor are serious about doing their jobs to question sources and recite context.  A newspaper doesn’t need to check its website for “hits” and then report the stories that get more hits: if it does that, then the stories will be exactly what you hear at a barbershop and a beauty salon and will have exactly that much importance and value.

My Dad talked back to his newspapers, but his only real dispute with a paper involved Curtis Russ, the long-time editor of The Mountaineer, sometime back in the 1960s. No one remembers what the row was about, but after Pop sent a letter to the editor to Mr. Russ, Pop quit the exchange, repeating to me an old Washington adage: “Never argue with a man who buys his ink by the barrel.”

(Frank Queen is an attorney who resides in Waynesville. He can be reached at This email address is being protected from spambots. You need JavaScript enabled to view it..)

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1 minute reading time (185 words)

New Owner of Wyoming's Sleeping Giant Ski Area Planning Improvements

SleepingGiant News owners plan to invest $2.5 million in the ski area over the next five years with a potential $1 million to be invested for this upcoming winter season.

After one ski season under his belt, Sleeping Giant Ski Area owner Nick Piazza is looking to get ahead of the curve by further investing in his mountain this summer.

“In my businesses, I know things are running well if we’re not talking about things for tomorrow or next week, but we’re talking about things for two months from now,” he said.

Piazza is forgoing the summer zip line season in order to focus on construction efforts for the mountain. Although the zip line has traditionally been the mountain’s biggest money-maker, he said it only makes sense to take a season off and come back in 2022 in a more prepared state.

“It never really got big enough to really solve the issues of the ski season,” he said of the zip line. “So we had to make kind of a bet, and our bet was we really care about the ski season, so why don’t we try to make that the best it can be rather than operate a whole other business and try to learn that again.”

 Read the full story at USNews.com
Vermont's Bolton Valley Bike Park To Resume Mounta...
Mt. Bachelor Plans New Summit Hiking, Biking Trail
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Home » Inspirational Catholic Social Teaching Quotes » Pope Francis » Page 5

Pope Francis

Catholic Social Teaching Quotes from Pope Francis

Learn about the social justice teachings of Pope Francis by reflecting on these quotes. Follow the links for the full text of the documents.

Love & Service

There is a world of difference between being drawn by love to serve others and promote the common good, and being driven by other forces, such as anger. What are the interior movements associated with my social action? It is possible to ‘keep all the rules’ and look like we… Read More »Love & Service

How might we build peace through our words, our hopes and our prayers? What are your hopes for peace?

Build Bridges

In a homily on dialogue, Pope Francis encourages us to make bridges and not to allow walls to arise. Who do you see in your community making bridges between groups rather than building walls? Pope Francis stresses the need for humility if we are to enter into dialogue. Have you… Read More »Build Bridges

Touching Poverty

Are we afraid to touch and be touched by the poverty of others? How might we do more than giving from a distance?

Costly Love

What might real love cost us? How might our Lenten penances help us to feel the pain of the poor and marginalized? Does the transformation of unjust structures require me to let go of a place of privilege?

Be Silent & Listen

The world of communications can be loud, frantic and busy. How do you make choices about who and what you take the time to listen to? What practices help you to be silent and listen to others?


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Evening Mom Wine Ankle Socks

Women's Evening Mom Wine Ankle Socks

Regular price $ 5.00 Sale

Sometimes you just need a drink!

Who doesn't like unwinding after a long day and having a drink? You can have another one, we won't tell the boss why you've got your lights turned down and the shades drawn tomorrow.

When you're wearing these amazing socks and that really boring meeting is droning on, you can just take a glance down and be spirited (pun intended) away to your happy place.

See our sizing guide for typical sizing information.

As always, for each pair of socks we ship, we'll be donating that same number of pairs to help individuals in need.

Size: Adult Female


text:
What are examples of landforms created by wave erosion?

1 Answer
Oct 27, 2015

Sea cliffs and terraces, sea stacks and caves.

Sea cliffs are steep faces of rock.
Terraces are flat form at the base of the cliff.
Sea stacks are columns of resistant rock that are left standing. If it a part of a sea cliff is made of less-resistant rock, when wave action erodes the rock sea caves, a hollowed portion forms.


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Lock Down Security: My Best Practices (and restoring usability)

I’m sure many of you are need to establish and implement a security matrix on your SharePoint sites. What do you need to do to ensure that your users can actually do the stuff they need to do? After all they are the business they are bringing the actual money home.

This is my first of probably many blog articles about SharePoint security. It is a rather complex and annoying issue that most of us don’t really want to think about too much. Generally most people solve it in one of three ways:

  1. Grant everybody some standard permissions and forget about it
  2. Grant (too) many administrative rights and trust them not to mess it up
  3. Lock down security and accept the loss of usability as many features stop working

I tend to go with either 1 or 3. For my more advanced setup the standard groups (option 1) doesn’t quite cut it and I’m stuck with option 3. That being said I do take pains to ensure that as much functionality as possible continue to work – the very purpose of this article.

I usually think of (and use) SharePoint Groups as roles and permission levels as role definitions. Note that the security setup has changed substantially since WSS 2.0, so you might need to read up on it (see bottom).

Designing the Security Matrix

When you need to implement security you quickly discover that there really is no way to import/export the security definitions and you are stuck with manual configuration. Sorry. It’s on my to-do list of tools that I would like (someone) to build. I know of only one way to “copy” the security setup from one environment to the next and that is a stsadm backup/restore of the site collection. It can be useable once or twice if you plan it right.

So what you need to do is to document and plan it thoroughly before you start. Nobody likes to do the same work 5 times over which you will otherwise end up doing. And sadly you’ll need to involve your client/customer/user and get them to buy in on whatever security you end up implementing. If you are like me you’ll want them to be able to maintain it after the project is delivered and then you need them to understand what you are doing, why and how they should use it in the future.

I prefer to use excel with a simple sheet with the site hierarchy as the first column and subsequent user groups/roles on the next columns. In the cells you simply write something the places where you’ll break the security inheritance and invent some labels that describe what that particular group should be able to do at that particular site. Don’t be too creative and don’t use a 20 letter encoding of the SharePoint permission levels. Use a label and map that to a custom permission level later (client probably don’t care about that).

It almost goes without saying that you should plan for as much inheritance as possible so the spreadsheet should be fairly sparse. If it contains lists or individual list items you should consider other options as that will certainly go awry in a very short time.

Deciding on AD or SharePoint Groups

So should you use SharePoint groups or should you use AD security groups (I’ll skip the discussion of AD group scope here – domain local, universal etc.)? You’ll probably end up with both as there are benefits and tradeoffs with both:

AD Security Group SharePoint Group
Contains both users and other AD security groups Contains both users and other AD security groups, but not other
SharePoint groups
Must be maintained in the AD, usually by IT department and/or IT administrators Permission to maintain groups can be assigned within SharePoint
Can be used (directly) within SharePoint for permissions Can be used for permission assignment (obviously)

And there are many more that I didn’t consider important here. Sharp readers will notice that I omitted server local groups here. Don’t ever use them. They are simply confusing, impossible to maintain and they don’t go well with a multi-server farm.

I usually prefer to have the AD deliver as much as possible, e.g. at least domain membership, probably department membership. I then assign those groups to a number of SharePoint groups that I use as roles and don’t worry too much about those. E.g. if you want to assign every user in the domain reader rights and a few (10%) some additional rights, then 90% of the users will be correctly assigned after adding “Domain Users” to your reader SharePoint group.

One word of warning: If your organization has been very creative in their domain setup and use excessively deep nesting in AD it might not work within SharePoint. It has been seen to happen. If you can assign that group properly to file share on the SharePoint server you should be quite certain that it also works in SharePoint.

The most important missing feature of the SharePoint groups is that they cannot be nested. It is a shame and the consequence is that you might need to add a given user to, say, 4 different SharePoint groups to make everything work. If it requires somebody to grant permissions on individual basis somewhere on the portal chances are that you should rethink your design.

Be sure to write down what is needed to grant a new user access to your portal that can be used as a reference for the SharePoint business owners at a later stage.

Managing Membership

One very helpful tip in managing the memberships of the SharePoint groups is that each group is assigned a owner that can adjust the memberships (by default), but it will also work if you assign a SharePoint group that role.

Go to the edit group page to modify it and set your custom SharePoint administrator group as owner of the group. From that point on your membership handling can be delegated to somebody without excessive rights e.g. farm administrators.

Implementing the Security Matrix

One thing that I’ll always recommend: Don’t use the standard SharePoint permission levels. They are well designed and therefore you should take a copy of them and assign the copy to your groups where you need it. That will enable you to later modify the permission levels in one place (as opposed to going through everywhere you assigned permission to that group) without compromising the integrity of the standard permission levels. Nothing is worse than a Contributor permission that actually grants administrative rights…

Ensure that the right people can administrate various group memberships by making sure who the owner of the SharePoint groups is.

When you implement (click through it) your matrix make extensive use of the “View Group Permission” feature (from settings menu when viewing group memberships, people.aspx). Another thing for my to-do list is to extend this feature to handle users as well – it’s not as hard as it sounds.

In the following I’ll cover some specific quirks that you might need to remember to ensure that your users can still use the site after you did your work (oh, they will resent you for it…)

Making the Galleries Work (Again)

The web part, list and site template galleries might need special permissions (if you try to limit access to the site collection root), to continue to work. To go to the permission setup, go to the actual galleries through Site settings, then gallery settings and finally the permissions.

  • Web Part Template Gallery: You should assign read permissions to this gallery to everybody (their group!) that should be able to add webparts somewhere. Otherwise the Add Web Part popup will be rather empty
  • List Template Gallery: Users that need to create lists from saved list templates obviously needs read access and the people creating the list templates should have contribute/write permissions.
  • Site Template Gallery: Users that need to create sites from the templates require read access. Users creating the site templates need contribute permissions on the gallery as well as the Site permission “Manage Permissions” (available on the permission level settings) on the target site that they want to save

Reuseable content

On a publishing web site, i.e. publishing or collaboration site, there is a list in the site collection root named “Reuseable content”.

If you want the functionality to work on any subsites you need to assign all editors contributor right to the list and all other users read permissions. If some users lack the permissions they simply won’t see that particular item of content on your publishing page. There will be no sign that they do not see the complete page, which tend to be pretty confusing to end users.

Content Types

Sadly there is no list or permission level that governs the customization of content types. You can’t prevent this through permissions if you also require some users to be able to manage your site permissions, i.e. add users.

You will want to prevent “people” (administrators) from changing all the content types that you defined in xml through feature deployment as that will disconnect your content type (see my two posts on that, 1 and 2) from the underlying xml source.

You can mark your content type as either read only or sealed with following tradeoffs

  • Read only: Will prevent accidental changes and allow inherited content types to delete/hide inherited columns. Administrators can actually override this setting if they really want to (but then they are not “in good faith”)
  • Sealed: Will prevent all changes through the GUI, but will also lock child content types so they can only hide inherited columns, not delete them

Both cases will not affect your ability to update the underlying xml files, i.e. it is actually not supported to change them after initial deployment. You can do it, but then you’ll need to update all inherited content types to preserve or restore their “inheritance” (see post). (A proper fix for this is another item on my to-do list)

Master Pages, Page Layouts and Styles

Just maintain the default “Style Readers” group and you should have no problems with these lists. If you do change the style readers group too much you need to ensure that

  • all users can read from the master pages catalog (“/_catalogs/”), otherwise they are completely blocked from your pages
  • all users can read from “/Style Library”

Final Words

I hope that these guidelines will help you a little bit. They helped me, but don’t for a minute consider them to be complete.

There are a number of features that require some special permission here and there if you tighten security on something else. It’s bound to happen and it is tedious and hard to test thoroughly.

Additional Resources

You can find some general information from Microsoft that defines all the basics and provides some guidelines here.

As usual Joel Oleson has something worthwhile to read on the subject.

About Søren Nielsen
Long time SharePoint Consultant.

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