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Reasons to Get Professional Packaging

Reasons to Get Professional Packaging 1

The use of artistic packaging drives attractive and eye-catching advertising. This is a way to encourage customers to buy products. It also convinces the customer that the product is also great. This is a competitive edge your product can have over products that are not well packaged.

The main reason for packaging helps to ensure the right audience is reached and boosts the level of your business. This proves to be a way of marketing that is cost effective and sales effective. Here are more reasons to go for product packaging.

Unique brand identity

It is worth noting that people’s opinion of the product depends on the way it is packaged. The brand also needs to match your product, for easier recognition. This is because the brand is the accurate representation of the product. This is, for instance, an image of the final product, in order for the client to identify with what they are buying.

Enhances business growth

When looking into business expansion, one factor to consider is changing the face of the product. This might be through new packaging design that will help to attain your aim. This, in turn, helps to facilitate the growth of your company. When the expansion is intended to move towards a certain direction, the branding adjustments have to go along. Once this is attained, the market margins start growing and thus, the market starts to grow.

Achieving target audience

When a product is meant for a specific audience, it is only proper to identify with them. This is based on the looks. The packaging is the best way to offer the target audience information and what to expect. Ensure the packaging is interesting, and also keeps the audience engaged and loyal to the product. Among other products, a professionally packaged product ought to stand out and be identified.

Bettering your profits

A properly packaged product has higher chances of flying off the shelves. This is the main reason why most companies are opting for product packaging. Impressive packaging helps to attain dominance among other products from your competitors. This results in better sales, thus, increasing the profit margins. It makes the investment you make worth it.

Higher business value

A company that has embraced product packaging also takes advantage of better value. This is mainly because the amount invested in packaging comes back more than five times the invested amount. This is the best way to oversee the growth of the company.

The main reason to go for professional product packaging is a stronger dominance of the shelf. Customer attention is drawn to products that are well packaged. Ensure the product is well represented, with great packaging; packaging that also represents the product that is inside, especially for high-quality products.

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Asphalt Paving Contractors for Commercial, Industrial, and Retail ApplicationsCommercial, industrial, and retail asphalt paving contractors are hired by property owners or managers to install new pavement and maintain the overall safety of the pavement in public parking lots and driveways. Regardless of the season, commercial properties need to always be in excellent condition so that they can be used safely and prevent accidents or vehicular damages from occurring. With that in mind, here are some important criteria that should be considered when hiring asphalt repair contractors in Toronto.

Best Practices for Commercial Paving Projects

Starting with the manufacturing process all the way to the repairs and installation, there’s a lot that goes into hiring a qualified asphalt pavement maintenance contractor in Vaughan and other parts of the GTA. Each property owner is responsible for ensuring that all asphalt repair projects are completed within a reasonable timeframe to avoid delays and loss of business in commercial, industrial, and retail spaces.

The longer commercial spaces are under construction or being repaired, the more frustrated people get due to the difficulty circumventing the construction sites. No one wants to lose valuable business because customers can’t access your property!

Asphalt Manufacturing Process

Every asphalt company has a different process for manufacturing their end product. Some companies use painstakingly thorough processes to get their mixtures to be just right, while others use cheap ingredients to speed up the process and save some money along the way. Choosing the right company means a guarantee that only the highest quality aggregates are being produced.

Proper Water Drainage

Another important aspect is to make sure that the property’s water drainage system is in good working order. If it’s damaged, then excess water will start to pool and eventually start to erode the asphalt, which can cause internal damages.

Good Compaction Techniques

Compaction involves pressing down on the soil where the asphalt mixture is going to be installed to make sure that it’s as perfectly even as possible so that there are no dips, depressions, or bumps in the final product once the asphalt dries. If the contractor fails to compact the soil or doesn’t do it properly, then the end result will be lumpy asphalt that buckles easily under pressure from precipitation and has tons of cracks. Cracks can eventually lead to potholes and other more expensive repairs that will eventually cost property owners more money.

Preventative Maintenance Process

Routine maintenance for parking lots and roadways that are used frequently is essential. In most cases, damages don’t necessarily have to do with the quality of the asphalt itself. Even the highest quality of asphalt aggregates can be susceptible to damages eventually if they’re placed in areas where there’s constantly heavy traffic or inclement weather. For that reason, it’s important to have contractors on standby to repair any minor damages that may occur such as hairline cracks before they get worse. On top of that, asphalt pavement should be sealcoated at least once every two to three years to help keep it fresh and functional for a long time.

Tips for Selecting an Asphalt Contractor for Commercial and Industrial Projects

Here are some important tips that can help you make the right decision when hiring an asphalt contractor.

Review Qualifications of Each Asphalt Paving Company

When looking for a commercial asphalt paving company, there are a lot of options to choose from. Rather than feeling overwhelmed in terms of making a decision, take the time to review all of their qualifications. Find out how long they’ve been in business, how much experience their crew members have, and what types of projects they’ve worked on in the past. All of these details should give you a good indication whether or not they’re suitable for the job.

Examine Materials and Equipment Being Used

The grade of the materials and equipment the contractors use is almost as important as the set of skills they bring to the table. Make sure everything they’re using is completely up to code and that none of it is outdated or in a state of disrepair before allowing them to commence their work. This will save you a great deal of money in the future because the quality of their work won’t constantly be called into question.

Always Hire a Licensed Local Contractor for the Job

Licensed asphalt contractors are specially trained to handle even the toughest repairs and installation projects. They typically have many years of experience working in a specific area and are very well familiar with the usual weather patterns there.

Make Sure Contractors Have Full Insurance

Asphalt contractors should offer full worker’s compensation to all of their employees in case any injuries or accidents occur while on the job. Before you even consider hiring a specific company, be sure to ask them about what type of insurance they have and exactly what it covers. That way you can guarantee that you’ll be absolved of any and all liability in case an injury does happen during the course of the project.

Educate Yourself about Important Industry Certifications

The more knowledge you have about mandatory contractor certifications versus the ones that are simply considered a bonus on someone’s resume, the greater the chances are that you can make a more informed hiring decision. Don’t hesitate to ask the companies on your shortlist about what types of special skills or certifications their employees have because this is also a testament to their level of experience and expertise on the job.

Commercial Asphalt Paving Contractors in Toronto

Your search for “asphalt paving contractors” or “crack sealing contractor in Toronto” ends here. Sure-Seal Pavement Maintenance Inc. is your one-stop shop for all of your asphalt installation and repair needs including pavement line striping contractors in Toronto. We’ve been serving commercial properties throughout Toronto and the GTA for well over 20 years and have always produced high-quality and long-lasting results for all of our clients. To learn more about our products or services, please contact us!

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26 August 2018 Susannah

Companion Guide – Nancy Mitford & ‘Love in a Cold Climate’

Nancy Mitford Love in a Cold Climate

Come with me to Mitford-land and the amusing, entertaining and madly eccentric world of Love in a Cold Climate. There you will find many weird and wonderful ways of loving, you will experience country house parties, learn about snobbery, and revel in gossipy and often bitchy conversations about a memorable, but rarely likeable, collection of people.

I am a huge Mitford fan – their lives, their novels, their letters, all fascinate me. Nancy’s wry, acerbic novels, Jessica’s memoir of their childhood, Debo’s books about Chatsworth and Diana’s autobiography – they are all so wonderfully eccentric and funny. A friend once told me that you could tell everything you needed to know about someone by asking them which Mitford sister was their favourite. I will confess here that mine is Nancy because she was the finest writer of the six sisters – make of that piece of information what you choose.

I find that whenever I give talks about the Mitford Girls, I am not alone in my fascination. The Mitfords continue to draw audiences and readers and to give delight through their extraordinary lives and their hilarious books. I hope you will be one of those who shares my delight in the Mitfords.

There are so many fabulous books about the Mitfords to read – Mary S. Lovell’s excellent The Mitford Girls, Laura Thompson’s Take Six Girls and the various published volumes of letters written by and to Nancy and Debo, such as In Tearing Haste: Letters Between Deborah Devonshire and Patrick Leigh Fermor. Learn more about Nancy’s life and loves, the film versions made of The Pursuit of Love and Love in a Cold Climate, and enjoy questions for discussion and food for thought about debutantes, dances and dalliances.

Nancy Mitford Love in a Cold Climate

Click here to purchase Susannah Fullerton’s Readers Guide to Nancy Mitford & Love in a Cold Climate.

Here are some convenient links for Nancy Mitford & Love in a Cold Climate.

   Love in a Cold Climate by Nancy Mitford
    Love in a Cold Climate by Nancy Mitford, 1980 Thames TV Series adaptation

   Nancy Mitford: A Memoir by Harold Acton
   Nancy Mitford: A Biography by Selina Hastings
   The Mitford Girls by Mary S. Lovell
   Take Six Girls: The Lives of the Mitford Sisters by Laura Thompson
   The Horror of Love: Nancy Mitford and Gaston Palewski in Paris and London by Lisa Hilton
   A Bookseller’s Way by George Haywood Hill, Anne Hill and Jonathan Gathorne-Hardy
   1939: The Last Season by Anne de Courcy
   The Bolter by Frances Osborne

  Susannah Fullerton: The Mitford Sisters
  Susannah Fullerton: HAPPY BIRTHDAY – Nancy Mitford, born 28 November 1904

  Nancy Mitford

I only recommend books I have read or know. Some of these links are my affiliate links. If you buy a book by clicking on one of these links I receive a small commission. It doesn’t cost you anything extra, but does help cover the cost of producing my free newsletter.

I always love to hear what you think.

Leave a comment.

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Comments (2)

  1. Alexandra (Lexie)Young

    Hi Susannah,
    I loved those books, “Love in a cold Climate”. and “the Pursuit of Love” joyous. I must read again as you encourage, re read these wonderful novels.
    I noticed you had a Pamela Hansford Johnson in your list.
    I read most of her books years ago when I lived in London. I would go to the local library where they had a collection of her books. I just loved them and I also loved her husbands books C.P Snow.
    I was a nurse and when I worked at the Brompton hospital in London she was a patient there.
    I was only young and wanted to ask her about one of her books and how it ended but I was a bit shy of “Lady Snow”.

    • Susannah Fullerton

      Wow, you met Pamela Hansford Johnson! How amazing. That also makes you only one remove from Dylan Thomas. I have read no CP Snow, so you have reminded me that I must remedy that lack.Do re-read Nancy Mitford’s books – they are well worth it.

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Sweat + The Ground Present JENS LEKMAN Saturday 1/27!

Happy 2018! We’re beyond stoked to lead off the new year with the announcement that we’re helping bring Swedish indie/pop/disco crooner JENS LEKMAN to Miami for the first time ever!

For those not familiar, Jens is a college radio favorite and Sweat best-seller who has released four very well-received albums (two of which were named “Best New Music” on Pitchfork) and several EPs, mostly on the Secretly Canadian label. His latest album Life Will See You Now came out last year to universal acclaim.

Join us at The Ground (aka the ground floor of Club Space on 11th St) for an intimate solo show with Jens on Saturday, January 27th. It’s an early show and will be over by 11pm. Opening up are Peter Oren and local faves SMVT so get your tickets now!


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Laser teeth Whitening Techniques – what You Ought To Know

You furthermore find how the cleaning of the aforementioned products could be expedited by following a simple rule. Avoid drinking liquids that stain such as coffee or tea. In case you must consume them then use a straw. It directs the flow of liquid at night front of your tooth.

These are commercially available pastes which contain mild abrasives to rub off the surface stains try not to have any active contents that chemically act to lighten pearly whites.

When leaping whiter teeth, do not be fooled by the different kinds of toothpastes offered. Toothpaste that claims to whiten your teeth will likely be more expensive than normal toothpaste. When attempting to remove stains from your very own teeth, regular toothpaste works just too and is frequently cheaper than whitening mouthwash.

I whiten my teeth often to bear in mind the lasting effect on the process. Haven’t got the time do not understand Confident Bright Smile Review Bright Smile that whitening your teeth isn’t a once only deal. Even though you check out the dentist to get it done, it won’t last. After time, sometimes as soon as 30 days, all teeth whiten processes, personal home and in office, will fade. Stains can reoccur and teeth can begin to get dingy again.

That is not to state that the different kind of removable partial dentures is not stable. The fixed bridge offers more stability this is because is fixed and inflexible. The crown and bridge the particular crown location the dentures into lay down. Only a dentist will capability to to tell you what essential. That is the reason why it is better to request a cosmetic dental work dental practice when the in necessity of a significantly more healthy mouth for ladies brighter Confident Bright Smile. Learn will be outstanding!

Do-it-yourself bleaching teeth techniques aren’t 100% clear of risk. One of the most common mistakes people make uses too a great deal of a whitening product or repeating the procedures instead of. In fact, if the whitening product you’re using is extremely strong, just end up permanently harming your dental health. You may cause damage that is irreversible. Always follow producers guidelines for applying the tool.

Let us not ignore the promotional videos for the teeth whitening system with the cliched voice over, nice-looking woman who knows a thing or few. She apparently knows what she is talking about in the 30-minute marketing spot that should have been only 20 seconds.

Coconut oil is creating a miraculous comeback after generations of undergoing treatment as a ”bad oil”. Today there is no limit to what amount of health benefits that can be touted to. It can be used as being a hair conditioner, skin lotion, sun block, sun tan enhancer, healthier oil for cooking, enhance weight loss, heal infections. It is reported to help with diabetes by controlling glucose. It is being studied as the benefit to Alzheimer patrons. It is good for the liver, the kidney, the heart, the pancreas and bone tissue!


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Chain Hoists And Their Various Types

A chair: A solid chair option to many device for stepping along on, or as basics to boost your employees resistance of callisthenic practices. If carried out long enough, stepping is often a great aerobic and general metabolic rate will activity. Other exercises, like push-ups and dips can be accomplished effective utilizing a chair.

The ceiling can additionally be used with ceiling shelves and Hoists. These support with storage of large items. lifting examination software may fit nicely for like wheel barrows.

Edmund Hill Shea, Jr .. was born August 15, 1929 in Portland, Oregon. Within his early years, he lived in the San Francisco Bay Area where his father managed the consruction for the piers of your Golden Gate Bridge. In 1935, his family moved back to Los Angeles. He attended Loyola High School, where he graduated in the late 1940s.

A back pack: Adding weight while walking boosts the effort required and consequently uses up more energy and fat to improve the value for the exercise. Compared to using hand or ankle weights, which lead to injuries your past elbow and ankle joints, put these in the rucksack and carry them around lying on your back.

A workout diary certainly a vital piece of lifting examination software equipment. Is actually usually always critical that you actually keep track of your improvement every week so you just can observe you’re appearing. It’s important a person know in which way much you lifted a few days before, as the your primary goal to continually lift more invest in. In order to be certain, you have to have to write all this down any exercise journal.

If your work requires sitting at a desk a lot, really important to change position to prevent back stretch. Take small trips to do things as up to possible, or even get up and expand. Try to move around every 30 minutes or in order to keep your back fulfilled.


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Filing Taxes – Lost Your Taxes? Here’s How To Replace It

Going Galt, for those not your past know, defines the main character in Ayn Rand’s Atlas Shrugged who leads the nation’s best and brightest business brains right work stoppage against the political sorts. They simply walk far removed from their firms, allowing in order to fail, leaving nothing for your tax-gobbling government-huggers to gorge themselves when.

The first part with the audit management software app for you to carry out a document review to find out if the quality/environmental manual exists and covers the requirements of the standards. Once this is productive you will then concentrate onto the company functions.

Don’t overlook YouTube. May to focus your marketing strategies marketing campaign here. YouTube presents free video enterprise. You can too really benefit from being upon the third most highly-trafficked site anywhere. This is the commonly visited video website near the net, and then it has the fame becoming the second most regularly used search engine for movie clips.

So, what else could you include? FIRS t, include you’ll likely notice the internal revenue service sent the individual IRS . Second, your legal name, address, social security number and year among the tax return or form in demande. Third, a short line or two regarding why the government is wholly wrong. Fourth, what you want the internal revenue service to do about the house. Finally, include copies of any documents aid prove your point.

I need to regarding my keep away from by investing a new maybe interesting spin to write-up. How one of my businesses could help you put money programs the pockets of companies by auditing utility statements. The other one helps folks caught in industry mess by auditing homeowner’s loans. It’s funny how my fingers on this keyboard did actually take on the life of their very own. I simply wanted to read more business. This particular seemingly sprang up from the no even. But I guess of which may be not possible, these thoughts were inside me somewhere, audit management software app asking to be removed. Kinda a good author’s exorcism. My business can wait as I explore innovative found creative ideas.

2) Running the point out. Often consultants find themselves unwilling or unable collect the necessary support they should to complete big programs and run their businesses at one time. There are mailings to do, events to plan and oversee and speeches to use. Without employee administrative and vendor support, running a profitable consulting business could absorb these runners hours and days you’ve allocated for leisurely attempts.


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How To Economize And Energy With Led Bulbs

There location you do today concerning your goals or dream. Writing it down is a first rate place begin. The next step is to attract up an approach for the next growth phase. Starting small gives you room realize on the job, making mistakes and correcting them as going. Starting small also allows the time to grow into who gemstones in order to handle where in order to heading.

We measured if our customers were happy our own services keeping score about our sexual performance. We agreed with our key customers a associated with measures or metrics, assigned a value for 1 and tracked them standard. We used little parking area traffic signalling systems on a specific sheet of paper use a quick visual representation of methods we did.

EXPRESS LANE DRIVERS – Another reputation for the express lane is ”passing lane” which perhaps more accurately describes the intent on the left lane on our highways. It disturbs me when it isn’t used for this reason. For example, some people get in the express lane and just the speed limit, in the event that. They act like a pace car once the danger flags are and also. I don’t know why they use this treatment other compared to what they want to deliberately irritate the people today driving around them. The kind of like them saying prissily, ”Well the cost limit is 55 and I’ll be damn if I’ll let anybody go faster than that.” I simply wish We a Jason bourne type of car the place that the rear axle would extend with knives on the conclusion to rip out their tires.

You learn things for you to do when attending a funeral, since never wearing brand new shoes, and in case you do, make damn sure you scuff bottoms of your shoes up well in the parking excellent. When new, sports shoes have the traction from a deer on ice, each and every you don’t need to can become flailing around like a drunken girl in a wet parking garage wearing new Jimmy Choo’s, keep reading to the foregoing. The time to achieve that you need to no traction is not while carrying five hundred pounds of dead excess! (Sorry, had to) One morning, we actually had to sling a coffin about 150 yards on dew dampened grass, along two moderate hills. You should’ve heard us talking later in the post-funeral lunch.

If you then have a taste for live alternative music may perhaps parking lot wander down Wilshire Blvd and browse the El Rey Theater had been once a craft deco movie that is turned perfect into a great live music spot.

That jogged his wandering mind and the man asked us if there were heard system bird call CD. Skippy rolled his eyes once the ex stupidly said no, and before we knew it, we had been blasted by blaring bird screeches, gobbles, and twitter posts. Skippy was most important to watch a battalion of rather large birds crossing the road heading toward our marketplace. Astonished that the CD obviously worked, Chuckie, Skip as well as the ex sprang into pastime.


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What do you do when lifestyle hands you lemons?

I don’t know on the subject of you, but We make pie. I QUICKLY eat 1 / 2 of it, decide it’s too good to talk about, and state I forgot to take it over for Easter. Oops. Positive thing my Mom made a lemon meringue pie for the family or there could have been tears. Seriously, they’ve got mad like for butter and sugars.

Cooking lemon pie brings memories of my Grandmother back again to me. She produced an incredible lemon pie with sky-high meringue. It was sensational, just like her. She was also very snippy and demanding at times. I believe she mostly lived off of espresso and buttered toast, and the casual chili from Wendy’s. I recall once she place a pizza within the range and asked me why it appeared so funny… it was upside down. Accurate story. I miss her laughter, attitude, which lemon pie.

I like pie. Like reallllllllyyyyy like it. I made this pie out of memory space of my grandmother but also because I had fashioned this crazy craving for lemon, sugar and butter. I’m convinced this pie is a complete breakfast, I imply… there are eggs inside it. Matters for something.

The pie was incredible but the secret may be the crust. This crust will make a pie baking goddess, and it’s so simple!It’s created from pecans and gingersnap cookies.

Ginger and lemon? Yep. Ugh, I desire you could reside in my mind. The flavor combination is perfect. Virtually no time for questions, obtain baking!

Place your gingersnaps and pecan within a food processor or blender. Process until they resemble great crumbs.

Insert melted butter and glucose. Process again.

Press your crust into a pie pan. Devote the oven and bake. Look extra adorable while carrying out it… easy!

The filling is pretty simple: fresh lemon juice, eggs, sour cream, sugar, and corn starch. Blend together and put into the baked pie crust. Place back into the range and bake again.

Spread refreshing whip cream on top. Now you’ve got a lemon pie all to yourself. Good luck.

I actually certainly can’t blame you for not posting.

Prep period:

10 mins

Cook time:

45 mins

Total period:

55 mins

1 cup cut pecan

1/2 cup dark brown sugar

1/4 cup butter, melted

1 tablespoon cornstarch

1/4 teaspoon salt

For the topping

1 1/2 cups heavy cream

1/4 cup sugar

Preheat oven to 350 levels F. Place gingersnap cookies and pecans in meals processor chip and pulse until they become fine crumbs. Add melted butter and dark brown sugar to meals processor with crumbs. Pulse again until the cookie crumbs resemble moist sand. Add flour and pulse once or twice even more until well combined. Scrape into 9 in . pie pan and work with a spoon or fingers to press into the pan; making sure the crust can be also. Bake in range for 10-12 mins. Place on wire rack to awesome.

In a big bowl, whisk jointly the eggs, sugar, sour cream, cornstarch and salt; gradually whisk in lemon juice and blend until well combined. Pour mixture in to the crust. Bake until arranged, about 30-35 moments. Cool pie totally.

To make the whip cream place 2 tablespoons cool water in a small saucepan, and sprinkle with gelatin; let soften about 5 minutes. Temperature mixture over very low warmth, stirring, until gelatin dissolves.

In a big bowl, using an electric mixer, beat cream and sugar until extremely soft peaks form. While defeating, gradually add gelatin mix, and continue beating until smooth peaks form. Utilizing a plastic spatula, pass on over cooled pie. Refrigerate until ready to serve.

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Tag Archives: Radio Chips

Radio Waves: Shedding Light on the Hindenburg, Chip Shortages, NPR at 50, and July 4th SAQ Grimeton Transmission

Icom IC-756 Pro Transceiver Dial

Radio Waves:  Stories Making Waves in the World of Radio

Because I keep my ear to the waves, as well as receive many tips from others who do the same, I find myself privy to radio-related stories that might interest SWLing Post readers.  To that end: Welcome to the SWLing Post’s Radio Waves, a collection of links to interesting stories making waves in the world of radio. Enjoy!

Many thanks to SWLing Post contributors Ron, Rich Cuff, and the Southgate ARC  for the following tips:

Radio Amateur’s Vintage Home Movie Film Sheds Light on Hindenburg Disaster (ARRL News)

Vintage home movie film provided by New Jersey radio amateur Bob Schenck, N2OO, was the highlight of a PBS documentary about the Hindenburg disaster. The film, shot by his uncle Harold Schenck, may provide clues as to what initiated the disastrous 1937 fire that destroyed the airship Hindenburg and claimed 35 lives as the German zeppelin was landing at Lakehurst, New Jersey. Harold Schenck tried to interest government investigators in his film, shot from a different angle than newsreel footage that begins only after the fire was well under way, but it was largely overlooked. “Nobody ever asked for it,” Bob Schenck explains in the documentary.

The Schenck film is the highlight of a PBS “NOVA” documentary, Hindenburg: The New Evidence, that investigates the issue in considerable depth in an effort to unlock the secrets of the cold case. The program aired on May 19 and remains available for streaming.

“My dad had bought this nifty Kodak camera — a wind-up movie camera, 8 millimeters — and he couldn’t come [to the Hindenburg landing] because he worked,” Bob Schenck recounted during the documentary. “So, he asked my uncle and my mom if they would take some shots and see the Hindenburg land.”

Bob Schenck approached Dan Grossman, an expert on airships, including Hindenburg, in 2012 during a commemoration of the disaster that forever memorialized radio reporter Herbert Morrison’s plaintive on-air reaction, “Oh, the humanity!” The NOVA documentary not only shares Schenck’s footage, which provided new clues to re-examine the cause of the explosion. The documentary also reviews scientific experiments that helped investigators come to a fresh understanding of what set off the fire. [Continue reading…]

Will chip shortage hit ham radios ? (Southgate ARC)

Glenn O’Donnell K3PP of Forrester Research notes the chip shortage may have a more serious impact than first thought and gives Amateur Radio rigs as an example of what might be affected

Self-described as a “ham radio nut,” O’Donnell discussed one of his hobbies to explain how the sway of tech titans could impact smaller companies as industries compete for limited resources.

“In this hobby, the newer radio “toys” are advanced technology, but the hottest radio might sell 5,000 units per year. If Apple wants 100 million chips, but the little ham radio company wants 5,000, Apple wins!” O’Donnell said.

Read the article at

NPR at 50: A Highly Selective History (Washington Post)

The network’s half-century evolution from an audio experiment to a media powerhouse

Today NPR is one of Washington’s most familiar and influential media companies, operating out of a gleaming, ultramodern broadcast facility on North Capitol Street. Its radio programs, online content, and podcasts reach millions of people around the world. But when it launched 50 years ago, in April 1971, National Public Radio was a decidedly scrappy enterprise.

How did a modest radio project from a bunch of audio idealists evolve into the multimedia behemoth that we now spend countless hours listening to? To celebrate NPR’s anniversary, we’ve put together a look at its history and transformation. Please note: If you would like to imagine the whole thing being read to you in the voices of Nina Totenberg and Robert Siegel, we won’t object. Click here to read the full article…

SAQ Grimeton Transmission on July 4th (Southgate ARC)

The annual transmission event on the Alexanderson Day with the Alexanderson Alternator from 1924, on VLF 17.2 kHz CW with the call sign SAQ, is scheduled for Sunday, July 4th, 2021.

The Alexander Grimeton Association are planning to carry out two broadcasts to the world from the old Alexanderson alternator SAQ. Only required staff will be in place, due to the ongoing pandemic.

Transmission schedule:

  • Startup and tuning at 10:30 CET (08:30 UTC) with a transmission of a message at 11:00 CET (09:00 UTC)
  • Startup and tuning at 13:30 CET (11:30 UTC) with a transmission of a message at 14:00 CET (12:00 UTC)

Live Video from World Heritage Grimeton Radio Station
Both transmission events can be seen live on our YouTube Channel.
The live video starts 5 minutes before the startup and tuning.

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Cornell engineers invent two-way radio on a single chip

Many thanks to SWLing Post contributor, Tom Ally, who shares the following news via the Cornell Chronicle:

Engineers devise two-way radio on a single chip

Two-way communication requires, of course, both send and receive capabilities. But putting them in the same device requires a filter between the send and receive circuits to provide signal isolation.

Without a significant filter, communication would be impossible.

“Your transmit signal is [10 to the power 14] times stronger than your receive signal,” said Alyosha Molnar, associate professor of electrical and computer engineering (ECE). “That’s 100 trillion times stronger – that’s a really hard problem.”

But researchers in Molnar’s lab have offered up a solution.

Molnar and collaborator Alyssa Apsel, professor of ECE, have devised a method for both transmitting and receiving a radio signal on a single chip, which ultimately could help change the way wireless communication is done.

Their work is described in “A wideband fully integrated software-defined transceiver for FDD and TDD operation,” published online Jan. 27 in the Institute of Electrical and Electronics Engineers’ Journal of Solid-State Circuits. Doctoral student Hazal Yüksel and Dong Yang, Ph.D. ’15, are co-lead authors.

Separating the send and receive bands is difficult enough, but the problem is compounded by the ever-increasing number of bands in the latest devices. From GPS to Bluetooth to Wi-Fi, each band requires a filter to stop the strong transmit signals from drowning out reception.

Molnar and Apsel have come up with an ingenious way to separate the signals. Their idea lies in the transmitter – actually a series of six subtransmitters all hooked into an artificial transmission line. Each sends its signal at regular intervals, and their individually weighted outputs are programmed so that they combine to produce a radio frequency signal in the forward direction, at the antenna port, while canceling out at the receive port.

The programmability of the individual outputs allows this simultaneous summation and cancellation to be tuned across a wide range of frequencies, and to adjust to signal strength at the antenna.

“In one direction, it’s a filter and you basically get this cancellation,” Apsel said. “And in the other direction, it’s an amplifier.”

“You put the antenna at one end and the amplified signal goes out the antenna, and you put the receiver at the other end and that’s where the nulling happens,” Molnar said. “Your receiver sees the antenna through this wire, the transmission line, but it doesn’t see the transmit signal because it’s canceling itself out at that end.” […]

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The user analysis of the fine operation of e commerce

fine operation, improve operational efficiency, enhance the core competitiveness of enterprises is increasingly and widely in the electricity business enterprise gradually action, just last month, we have done a "fine operation" proposal, today my spare time and friends to discuss together, is more valuable I hope, and the vast majority of peers to explore together. The main framework of the program as shown below, as a result of the proposal is very long, so today to share with you the user analysis, positioning, value mining, fine operation. I will share the other content to discuss.

electricity supplier joke:

a rich man wants to marry his wife, there are three candidates, the rich gave the three girls each $one thousand, please fill the room. The first girl bought a lot of cotton, filled the room with 1/2. Second girls bought a lot of balloons, filled the room 3/4. Third girls bought candles to fill the room with light. In the end, the rich chose the biggest one. The story tells us that it is important to understand the real needs of the customer.

a, user positioning

The essence of

in any business is to "meet customer expectations" and "beyond expectation", the first MIW to know what users love? How to meet their needs? Their behavior? Their purpose? Everyone’s interest, hobbies, personality, culture, economic situation is not the same, in the purchase of psychological It differs from man to man. also. So the formation of a variety of buying motives.

"only to help users achieve their goal of shopping, MIW can truly realize their goals; we think we should pay attention to the user experience in the mall before the project comprehensive promotion, for user’s convenience and derivative loyalty. The following figure shows the methods and contents of MIW user analysis:

two, data analysis

when MIW users in the E-commerce mall with the purchase behavior, the potential customers from the mall into the value of customers. The transaction information of the user MIW e-commerce platform, including the purchase time, the purchase of goods, purchase quantity, payment amount of information stored in its database, we can store the operation data to analyze transactions based on their users to estimate the value of each, and for each user to expand marketing (Lead Generation the possibility of).

3 principles to evaluate user value:

(1) indicators can be quantified;

(2) as comprehensive as possible: according to the underlying data to select as many indicators can be obtained, so that can be analyzed from many angles and evaluation;

(3) linear independence: that is, as far as possible between the indicators remain irrelevant. For example, if the number of users to choose the number of purchases and the total amount of consumption, it must be the number of users to buy more the higher the total amount of consumption, which is the result of the evaluation dimension >

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text:
H&H - Cashew & Mango Nut and fruit bar

Serving Size :
1 bar

Daily Goals

How does this food fit into your daily goals?

Calorie Goal1,840 cal
160 / 2,000 calleft
Fitness Goals :
Heart Healthy
6 / 67gleft
45 / 2,300gleft
-- / 300gleft

Nutritional Info

Carbs23 g
Dietary Fiber5 g
Sugar10 g
Fat6 g
Saturated1 g
Polyunsaturated0 g
Monounsaturated0 g
Trans0 g
Protein4 g
Sodium45 mg
Potassium0 mg
Cholesterol0 mg
Vitamin A2 %
Vitamin C4 %
Calcium4 %
Iron8 %

Percentages are based on a diet of 2000 calories a day.

Activity Needed to Burn:

160 calories

24Minutes of Cycling
16Minutes of Running
58Minutes of Cleaning

Recipes & Inspiration

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So just what is the estimation of uncertainty of measurement? I am so happy you asked. While the term might take you back to the old acronym ‘WAG’ (Wild Ass Guess) the International Organization for Standardization ISO 17025, “General Requirements for the Competence of Testing and Calibration Laboratories” has emphasized the mandatory requirement for all testing & calibration laboratories to apply procedures for close ‘estimation of uncertainty measurement’. It is compulsory for testing & calibration laboratories when submitting final test /calibration results; labs should also attach an enclosed statement about the best closest estimated uncertainty of measurement, keeping it most relevant to the validity of results. All the general requirements for the estimation of uncertainty of measurement and reporting has been mentioned in ISO 17025.

Definition of Uncertainty in ‘Estimation of Uncertainty of Measurement’ as Defined by ISO

Uncertainty – is defined by ISO (International Vocabulary of Basic and General Terms in Metrology) as the parameter, associated with the result of a measurement that characterizes the dispersion of the values that could reasonably be attributed to the measurement. Got that? (Redundant question!)

Responsible Functions of Testing Bodies

Let’s start with the responsibility of functions:

Quality Assurance: QA is responsible for maintaining and performing the activities defined in the estimation of uncertainty in the measurement procedure.

Lab Operations: All Lab operators are responsible for ensuring that all the specified requirements should be followed by all means.

Other Supporting Functions: All other relevant functions are responsible for ensuring that all the requirements specified in the procedure has been fulfilled or not.

Why Calculate Uncertainty of Measurement?

The ultimate use of estimating uncertainties in measurement is to:

  • Be able to exhibit about the accuracy of test results that have been performed in the lab by lab operators.
  • Allow the consumer of data to be able to interpret data in the report properly.

While estimating the uncertainties, it is important to take into account all possible contributors that can affect your lab testing activity and on the uncertainty of measurement.

  • After measuring uncertainty in measurement, one can get a realistic comparison of outcomes from different testing/calibration bodies.
  • Measuring uncertainty in measurement often helps to avoid repetitive testing/calibration procedures, thus saving time and cost.
  • While evaluating uncertainties in measurement, one always count all the factors that can impact or contribute towards uncertainties in measurement, leading to improvement of the procedures, making testing/calibration bodies more precise.

General Principles of Measuring Uncertainties of Measurement

  1. Know More About Measurand: The main objective of doing measurement is to know the value of measurand (anything which is being measured). It is important for measurement to begin with an appropriate set of specification of the measurand, a proper method of measurement, and the specific detail of the measurement procedure.
  2. Perfectly Perfect: No measurement process or testing /calibration can be called perfectly perfect! Also, those imperfections help uncertainties to rise ultimately leading to an error of measurement in the final result. The result of any measurement being carried out has some certain quantity of uncertainties, which are important to get identified.
  3. Components of Uncertainties: Random and Systematic components always exist in case of errors. However, uncertainties arise from more random factors and imperfect calibration for systematic errors.
  4. Random errors: Random errors usually arises from variations being made randomly. Every time a measurement is being done, random factors impact uncertainties in different ways, varying in every measurement cycle coming from various sources each time the measurement is being taken.
  5. Systematic errors: Systematic errors arise from other systematic effects. However, these can be reduced to a much extent firstly by identifying them and then by eliminating (preferably) or reducing them.
  6. Components of Uncertainty of Measurement: All the components of the uncertainty of measurement should be evaluated using proper methods, and each is expressed as Standard Deviation and also referred to as Standard Uncertainty.
  7. Standard Components of Uncertainties: All the standard components of uncertainties combined to produce an overall uncertainty value, which is often referred to as Combined Standard Uncertainty.

Where Does Uncertainty in Measurement Come From?

There can be many possible sources of uncertainties in testing/calibration and measurements existing in testing/calibration labs, which can be stated as:

  • If the requirement of the test/calibration is not being defined clearly, then ambiguities may lead to uncertainties in measurement
  • Even if the requirements of the test/calibration is known even then, it is not possible to fulfill the required conditions for the testing/calibration bodies
  • Improper sampling methods may lead to uncertainties
  • Especially in analog instruments, personal bias can end up having uncertainty in measurement
  • The mistake in the values of constants and other parameters used in data evaluation
  • Errors in instrument scale
  • Variations in repeated observations can still be observed even if the test /calibration is being performed in the same conditions

Calibration is characterized as follows:

  • Repeated measurements can be made
  • Uncertainty of reference instruments is provided at each stage down the calibration chain, starting with the national standard, and
  • Customers are aware of the need for a statement of uncertainty to ensure that the instrument meets their requirements.

Basic Calculations

The measurement uncertainty calculation is:

Expanded Uncertainty = (combined Standard Uncertainty) * K

 Where K is the coverage factor.

estimation of uncertainty of measurement

Estimation of Uncertainty of Measurement

The combined Standard Uncertainty is an RSS (Root Sum Square) calculation:

U1 is the calibrator’s accuracy-related uncertainty.

U2 is the combination of two DUT related uncertainty components:

  1. S1: The standard deviation of the mean within a sequence of actual measurements (a Type A uncertainty)
  2. S2: The resolution (or sensitivity) of the DUT (a Type B uncertainty)

U3, U4, U5, U6, U7, U8, U9, and U10 are optional uncertainty components.

Uncertainty is an unpreventable part of any measurement, and it begins to matter when results are close to a specified limit.  A proper evaluation of uncertainty is good professional testing/calibration body practice and can provide laboratories and customers with valuable information about the quality and reliability of the result.

Although common practice in calibration, there is some way to go with an expression of uncertainty in testing/calibration; however, there is growing activity in the area and, in time, estimation of uncertainty measurement will be common. No more WAGs!

Looking to get ISO/IEC 17025 accreditation for your business?

What questions do you have and how can we help?


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You asked: What hormone does follicle produce?

It is released in a rhythmic fashion every 60 to 120 minutes. GnRH stimulates the pituitary gland to produce follicle stimulating hormone (FSH), the hormone responsible for starting follicle (egg) development and causing the level of estrogen, the primary female hormone, to rise.

What hormones do follicles secrete?

Residual cells within ovulated follicles proliferate to form corpora lutea, which secrete the steroid hormones progesterone and estradiol.

Do follicles produce estrogen?

Each follicle contains an egg. Later in this phase, as the follicle-stimulating hormone level decreases, only one follicle continues to develop. This follicle produces estrogen. The ovulatory phase begins with a surge in luteinizing hormone and follicle-stimulating hormone levels.

What does the follicle hormone do?

Follicle stimulating hormone is one of the hormones essential to pubertal development and the function of women’s ovaries and men’s testes. In women, this hormone stimulates the growth of ovarian follicles in the ovary before the release of an egg from one follicle at ovulation. It also increases oestradiol production.

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What hormone does the dominating follicle release?

Ovulation. When the level of estrogen is sufficiently high, it produces a sudden release of LH, usually around day thirteen of the cycle. This LH surge (peak) triggers a complex set of events within the follicles that result in the final maturation of the egg and follicular collapse with egg extrusion.

Which hormone maintains the uterus lining?

Progesterone maintains the uterus lining (the thickness of the uterus wall). If the egg has not been fertilised, the corpus luteum dies and progesterone levels drop. This causes menstruation, where the uterus lining breaks down – this is known as having a period.

How can I increase my follicle stimulating hormone?

A B6 supplement, along with B-vitamin rich foods, can also help to increase progesterone. Abnormal levels of FSH or LH can be balanced with daily vitex or white peony supplements, and they work best when prolactin hormone is also elevated.

Why do follicles not produce eggs?

The follicle may have ruptured (ovulated) prior to the egg collection. … This can result in the eggs being lost in the follicular fluid that is released from the ovary at the time of ovulation. 2. The doctor may experience technical problems during the egg collection procedure .

What happens when estrogen drops?

Low estrogen levels can interfere with sexual development and sexual functions. They can also increase your risk of obesity, osteoporosis, and cardiovascular disease.

How long does it take a follicle to mature?

Follicles in one of your ovaries start to mature between days 6 and 14 of your menstrual cycle. Around days 10 and 14, an egg develops from one of these follicles. This egg is then released from the ovary on day 14.

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What is the normal level of follicle stimulating hormone?

During puberty – 0.3 to 10.0 mIU/mL (0.3 to 10.0 IU/L) Women who are still menstruating – 4.7 to 21.5 mIU/mL (4.5 to 21.5 IU/L) After menopause – 25.8 to 134.8 mIU/mL (25.8 to 134.8 IU/L)

What are the symptoms of low FSH?

FSH and LH deficiency: In women, symptoms include irregular or stopped menstrual periods and infertility. In men, symptoms include loss of body and facial hair, weakness, lack of interest in sexual activity, erectile dysfunction, and infertility.

What happens if follicles don’t grow?

The follicles stop growing or arrest and become atretic remaining as small cystic areas seen on ultrasound. Since no egg matures or is released, ovulation does not occur and the hormone progesterone is not made. … The small arrested or atretic follicles produce male hormones with contribute to the symptoms of PCOS.

How many follicles are normal?

On average ten to twelve follicles are produced per cycle, but this can vary from person to person and is affected by multiple factors such as age, medical history and your individual response to ovarian stimulation.

Is a mature follicle that after ovulation becomes a?

During ovulation, a mature egg is released from a follicle. … After ovulation, the follicle turns into a corpus luteum.

Does dominant follicle mean pregnancy?

The present data were summarized as follows. A dominant follicle started to grow when LH. HCG levels declined to approximately 120 mIU l and FSH increased slightly. The largest nonatretic follicle seen in pregnancy or PCO was comparable with that seen in the late luteal phase of a normal cycle.

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Lots of iodine


text:
15 June 2021
A massive fire devastated hundreds of dunums of land between the villages of Laban, Al Jaryn, and Arika in the countryside of As-Suwayda
Easter Deir Ezzor countryside: an unidentified body was found in Hawi al-Hawaij, killed by a gunshot.
Prisoners swap being conducted between HTS and SAA in Darat Azza, west of Aleppo
Opposition forces targeted a weapons depot in the village of Kafr Dael in western Aleppo countryside, probably belonging to Iranian forces present in the area, causing physical damage to the warehouse building.
Raqqa: Russia is sending a convoy of weapons and logistical equipment to its forces in the town of Ain Issa in the northern countryside
1 year ago
Syria: a major blast was heard last night in whole Aleppo-city. Reason & precise spot remain unknown (South-West countryside), local sources deny any airstrike was carried out
After explosion heard in Aleppo city, rumors that warplanes believed to be Israeli hit Iranian sites In Faculty of Artillery, from the southwestern side
1 year ago
Turkish Minister of Defense: Turkey has taken measures in cooperation with Russia against violations in Idlib and our aim is to permanently establish a ceasefire
Military police seize a car bomb intended for remote detonation in Akhtarine, in the northern countryside of Aleppo
Deir ez-Zur: Unidentified people target with automatic weapons and RPG shells, the government's militia at Ma`bar Qurs crossing on the opposite bank of Euphrates
Pro-Assad forces target with tank shells Majdalia village, south of Idlib
Lots of crops burnt today west of Girespi1 year ago
Lots of crops burnt today west of Girespi
Six civilians including a child were injured this afternoon as a result of an IED explosion in Al Bab City in Aleppo.
South Syria: two reconcilied Rebels were targeted tonight by gunmen in outskirts of Daraa. One was killed (photo) & the second severely wounded
Fires are on the international road m5 between the cities of Maarat al-Numan and Saraqib, south of Idlib
IRGC-backed Zaynabiyoun Brigade, which recruits Pakistani fights, holds religious ceremony in the eastern Syrian border town of Albu Kamal with the participation of Liwa Fatemiyoun
Video documenting the shelling from Asasd Barracks with heavy artillery on the town of Al-Barah, south of Idlib
YPG: on 13 and 14 May, HRE forces carried out a number of operations targeting Turkish army and its affiliated terror groups in Azaz, Afrin and Şêrawa. 14 Turkish soldiers and turkish-backed militants were killed and wounded, 3 vehicles destroyed
Groups of the National Defense Militia and Iranian militias Thursday combed the Albukamal Badia to search for ISIS cells deployed in the Badia area, and after they conducted the combing tour in the Badia they found a truck pickup belonging to the organization, allegedly by the militia
The burning of four tents for displaced people in Al-Salam camp near the village of Sheikh Yusef, west of Idlib, this afternoon, as a result of a gas leak during cooking in one of the tents, without any injuries to civilians
1 year ago
Russian Foreign Ministry: Russian Deputy Foreign Minister Verchinin discussed the situation in Syria with US Special Envoy James Jeffrey
Rebels are responding to the sources of attack on Al Bara and targeting with heavy artillery the locations of the pro-Assad forces in the city of Kafr Nabl and the village of Hazaren in Idlib countryside
A motorbike bomb exploded in the main street in Ashaddadi city in Hasaka caused material damage to some shops and stopped cars
Artillery shelling by the pro-Assad forces on the outskirts of Al-Bara town, south of Idlib, resulted in two dead and several wounded civilians
Pro-Assad forces launched a campaign of arrests in the city of Maadan under its control in the eastern countryside of Raqqa and arrested 7 suspects on charges of insulting foreign currency exchange
Pro-Assad forces target with artillery shells Qaledin village, West Hama
Pro-Assad forces stationed in Maarat Al-Numan city bombard with heavy artillery Al-Barah town and Sfuhun village, south of Idlib
Pro-Assad forces target with heavy artillery shells the towns of Kansafra, Al-Barah, Sufouhn and Al-Fateera in the southern countryside of Idlib.
1 year ago
Turkish forces burn fields in Sirzuri and Faris villages in the Tel Abyad countryside
Deir Al-Zour: daughter of Saleh Ahmed Al-Jazaa Al-Dawoud, from Al-Baghouz village, was killed in the refugee gathering points in Shuhail, as a result of the indiscriminate shooting in the city of Shuhail. The shooting turned out to be a problem between two families in the city, with the absence of SDF members
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text:
How do I choose an RNA-seq package?

What is the difference between RNA-seq options #121-124 and #133-134, and which should I choose?

#121-124 use poly-A pull-down for mRNA enrichment, while #133-134 use rRNA depletion to remove ribosomal RNA.

Both workflows retain strand information of the transcripts.

If your experiment's goal is coding gene expression profiling and you have good quality total RNA samples (RIN>8 by Agilent Bioanalyzer), you most likely only need packages #121-124.

If your RNA samples are degraded, it means that they are losing their poly-A tails. Therefore, no matter the goal of your experiment, you should choose #133-134.

If your experimental goal is not only coding gene expression but also lncRNAs, you should choose #133-134, regardless of your RNA quality.

Should I choose paired end (2 x 100 bp) or single read (100 bp) sequencing?

If your experimental goal is gene expression profiling, you most likely only need single read packages.

If you are also interested in detecting structural variations, alternative splicing patterns, or even point mutations, you should choose paired end packages.

What depth do I need?

For coding gene expression profiling of human/mouse/rat, the 30M single read option (#121) provides sufficient coverage.

Deeper sequencing is recommended if your genes of interest are expressed in lower quantities.

Because many non-coding RNA species are found along with mRNA, the rRNA depletion protocol requires deeper sequencing.

Why do some packages require pre-approval?

Some packages are designed for users with significant informatics experience. We can only offer them to users known to us who are able to handle the increased informatics workload without our help.

The packages that REQUIRE pre-approval are #120, #131-132, #401-402, #421, and #431. Please do not choose these without speaking with a member of the Genome Center first.

I don’t have 10 ng input RNA. Do you offer RNA amplification services?

If you have less than 50 ng of input RNA, we offer an option for RNA amplification using Clontech’s SMART-seq v4 Ultra Low Input RNA Kit to create amplified cDNA, followed by library preparation using Illumina’s Nextera XT kit. The minimum input required for this kit is 10 pg, but >200 pg is preferred. Please choose package #141.

If you have more than 50 ng input RNA but less than 100 ng (and RIN >8), we have had success with option #121 but cannot guarantee it will yield a sequenceable library with sufficient complexity. We can try this option and save 10% of your starting material to do option #141 in the event #121 doesn’t work.

We recommend choosing all option #141 if all of your samples are very low input; if you have only one or two low-input samples in a larger batch with enough material, choosing to try #121 for all samples (saving input material to do #141) is preferable.

Do you accept FFPE RNA for RNA-seq?

For degraded and FFPE RNA, we offer the TruSeq Stranded Total RNA with Ribo-Zero Gold prep (packages #133-134). This uses Ribo-Zero technology to deplete the rRNA (both cytoplasmic and mitochondrial), as the normal protocol will not work due to loss of the poly-A tails. We still require a Bioanalyzer report. We accept RINs of <8 for this protocol, but cannot guarantee quality as the quality of FFPE is so variable. Sequencing is required at a higher depth due to remaining non-coding RNA. (For this reason, we recommend starting at 60M paired-end reads).

How should I design my RNA-seq experiment?

Is it OK to collect samples in batches?

It is best if you can collect samples at once, but it is common practice that researchers collect/extract RNAs at different times using the same protocol.

Can I compare data across batches?

Please choose the same package repeatedly in order to compare different submissions. In addition, please let us know at the time of submission if you’d like to compare the current project with a previous project to ensure that we use the same tools for data analysis.

We also include an RNA spike-in control in each sample, which can be used for normalization.

Do I need biological or technical replicates? If so, how many?

Yes, biological replicates are strongly recommended. We suggest at least 3 biological replicates for each treatment/group. We do not make technical replicate libraries from the same sample, as next-generation sequencing technology itself is highly reproducible.

Submitting your samples

What are the submission requirements for my samples?

Please see Submitting Your Samples for information.

Do I need to do a Bioanalyzer? Will a TapeStation report be sufficient?

Yes (for RNA samples), a Bioanalyzer report is preferred. For RNA samples we require that the Bioanalyzer report not be older than a week at the time of submission. If samples have undergone a freeze/thaw cycle or have been shipped since then we strongly recommend rerunning the sample. We cannot provide a guarantee for data quality if the above conditions are not met.

A TapeStation report will suffice. However, we cannot provide a guarantee for data quality.

A Bioanalyzer report is not required for gDNA samples, but is required for cDNA samples.

How do I Bioanalyze my samples?

There are two options for Columbia users.

Researchers can send samples to the Molecular Pathology Core. If you dilute your samples, please indicate this in the sample name.

If you would like to purchase your own Bioanalyzer reagents, you may use our Bioanalyzer as a self-service instrument. Please contact to set up an appointment. This is recommended for advanced users only.

External users can submit a request for the Genome Center to perform the Bioanalyzer for them. This service is $30 per sample.

Custom projects/self-prepared libraries

What should I do if my request is not RNA-seq/exome sequencing/whole genome sequencing?

If you are interested in ChIP-seq, panel sequencing, or other applications for which we don’t have a full service, we recommend preparing your own libraries with Illumina platform-compatible protocols, and we will be happy to sequence them for you. Please see below for self-prepared library requirements.

What should I do with self-prepared libraries?

You will need to quantify self-prepared libraries correctly, as this is critical for the success of a sequencing run. We recommend using qPCR, or using the average fragment length from the Bioanalyzer with a fluorescent reading to calculate the molarity (Qubit or PicoGreen).

We require at least a 10 nM pooled library in 10 μl water. We will load the sampled based on your quantification. We require all index information at the time of submission (name of index and sequence) and a Bioanalyzer report of your pooled library. Please complete the Self-Prepared Library Sample Form and send it to

NextSeq self-service

How can I make use of your NextSeq service?

You have two options for accessing the Columbia Genome Center's NextSeq capabilities.

1. Have Columbia Genome Center staff run your sequencing.

We will charge you for what we pay for the kit plus labor (see Pricing).

We require at least a 10 nM pooled library in 10 μl water. We will load the samples based on your quantification. We require all index information at the time of submission (name of index and sequence) and a Bioanalyzer report of your pooled library.

 You can create a run on BaseSpace and share the login and password information with us, which allows you to download your data directly from BaseSpace. We can also run the data through our pipeline and provide basic data analysis.

2. Become a self-user of NextSeq instruments.

To become a Next-seq user please:

  • Review Illumina’s online tutorial videos and user guides.
  • Create or become familiar with your lab’s BaseSpace account.
  • Observe another self-user setting up a run. (If you do not have a lab member to observe, please contact us.)
  • Let us know when your first run is. If you are unassisted, we will help you set up the instrument. Please contact Illumina for BaseSpace set-up and support ( or 1-800-809-4566).

Once you have successfully completed your first run, we will add you to our user list and establish key-card access to the sequencing room.

Post-sequencing questions

What is your turnaround time?

Over the past year, our average turnaround time for standard RNA-seq has been 2-3 weeks from the time we receive your samples to the time we deliver the data.

If you need a project delivered by a certain date, please contact us and we can try to accommodate you to the best of our abilities. Please let us know this at the time of submission so we can plan properly.

What are your deliverables?

RNA-seq (human/mouse/rat/celegans/rabbit/zebrafish/yeast/dropophila)

FASTQ, BAM, counts table, and list of differentially expressed genes if sample group information is given

Whole exome sequencing (human)

FASTQ, BAM, and VCF (germline variants)

Whole genome sequencing (human)

How do you release the data? How long will you store our data? Can we get our samples back?

We will give you a web link to download the data. You are also welcome to bring over a hard drive and we can transfer the data to the drive.

Data will be stored on our server for 1 month before it is deleted permanently.

Remaining samples will be stored for 1 week before being discarded. Your data release email will include a note indicating that you can pick up any remaining sample material. We will store libraries for 6 months, after which time they will be discarded without warning.


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How to make TacoRito

Tacorito is a mild and meaty South-western dish that combines the delicious flavour of tacos with the twist of baked burritos. I you want to order Tacoritos from Tacovilla, then you can visit their website or reach the Tacovilla restaurant near you.

If you want to prepare this dish at home, then you can follow the below recipe that we have modified for your convenience. Before preparing this dish, you should arrange the required ingredients for preparing this dish.

A Tacorito from Taco Villa features ground beef along with some spices and flour tortillas. Let us know all the ingredients that you need to arrange while preparing this dish at home.

  • 1/4 cup butter, cubed
  • 1/4 cup all-purpose flour
  • 4 cups water
  • 3 tablespoons chili powder
  • 1 teaspoon garlic salt
  • 1 pound ground beef
  • 1 pound bulk pork sausage
  • 1/4 cup chopped onion
  • 1 cup refried beans
  • 8 flour tortillas (8 inches), warmed
  • 3 cups shredded Monterey Jack cheese

For Toppings:

  • Shredded lettuce
  • Chopped tomatoes
  • Sliced ripe olives
  • Sour cream

Method of Preparation

  1. To begin with this recipe, melt butter in a large saucepan. Add in flour until it gets smooth, while gradually adding water to the flour. Bring flour and water mixture to a boil; cook and stir for 1 minute or until the flour becomes thickened.
  2. Add in chilli powder and garlic salt. Bring all these things to a boil. Reduce heat; simmer for 10 minutes keeping the pan uncovered.
  3. Switch on the gas stove. Place a large skillet on it. Add oil and heat over medium heat. Add beef, sausage and onion to the oil.
  4. Cook until the meat is cooked properly. Drain the excess oil. Add in refried beans and cook for another 1-2 minutes.
  5. Add ¼ cup sauce in a greased baking dish. Place tortillas on it. Spread 1 tablespoon sauce over each tortilla; add 2/3 cup meat mixture down the centre of each tortilla. Top each tortilla with ¼ cup cheese.
  6. Roll up tortillas and place the seam side down in a baking dish. Add remaining sauce over the top; top with remaining cheese.
  7. Bake at 350° for 18-22 minutes while keeping it uncovered or until the cheese is melted.
  8. Serve hot with suggested toppings as desired.

Nutrition Facts

There are 390 calories in a Tacorito from Taco Villa; most of the calories come from carbohydrate and fat. Let us discuss all the nutrients that we get from 1 Tacorito from Taco villa.

  • Serving: 1 Tacorito
  • Calories: 390
  • Calories from Fat: 160
  • Total Fat (g): 18
  • Saturated Fat (g): 0
  • Trans Fat (g): 0
  • Cholesterol (mg): 40
  • Sodium (mg): 530
  • Total Carbohydrates (g): 37
  • Dietary Fiber (g): 5
  • Sugars (g): 0
  • Protein (g): 19

The % Daily Value (DV) tells you how much a nutrient in a serving of food contributes to a daily diet. 2,000 calories a day is used for general nutrition advice.

Nutrition Facts Source: www.tacovilla.com

Mrs H.

Gail, TX

I have always loved going to this Taco Villa whenever I could since I live out of town. I went there tonight and the tortilla on my burrito was crispy and not at all soft. The beans were lukewarm all of which made for a very undesirable meal. I threw the burrito away.

I had also gotten a Guadalajara which I thought would be fine. It was awful. The tostada was stale and the guacamole very undesirable. I also threw it away.

What a disappointment!  It has always been a favorite place. I don't know if I'll ever try it again.

I'm so sad.

Phillip H.

Tahoka, TX

We eat at taco vlla often but haven't been to this one in quite a few years. The taco salad was supposed to be shredded chicken but it was large chunks. The salad was also missing a few ingredients. The taco salad shell was stale and soft. We also got tacos and the shells were very stale and in appetizing. The ground beef didn't taste like taco villas ground beef either. They didn't make it the way all the others do. Very disappointing.

Michael J.

Irving, TX

I'm a huge Taco Villa fan, but this location needs some attention. First I stopped at the speaker to place my order. There was one vehicle in front of me at the window. They never took my order after 4-5 minutes. I keep saying hello, with no response. When the car at the window pulled away, I pulled up to the window. I looked into the window with three people working and two of them saw me and keep moving about without responding to me after several minutes. Finally a girl with the headset came to the window, opened it and just stood there staring at me. She said nothing, then I said "do I need to come in to order, did you hear me at the speaker?" She said" like it doesn't hear big trucks". Big trucks? I'm driving a Chevy Silverado, I told her I'll just go somewhere else. I will never go there again, nor another Taco Villa.

The Most Popular Queries

How can I save calories while preparing Tacorito at home?

Ans: You can save calories by reducing the usage of cheese in it. If you are really checking on your calories, you can skip using cheese or reduce the quantity of cheese in your favourite Tacorito.

Latest News

Taco Villa is closing its chain at Waite Park: The chain will have the last day on Christmas Eve.

WAITE PARK : A local favorite in the world of Mexican food is shuttering its doors, reported by WJON.

Taco Villa, located along Division Street in Waite Park, will have its last business day on Christmas Eve. Cole Friel has worked at Taco Villa for 8 years alongside his mother, who has worked there for over 20 years. He says the decision to close the restaurant was based on necessity.


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Regular price $60.99

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Nature's Logic - Chicken 15.4lbs

Nature’s Logic Canine Chicken Meal Feast is a savory blend of chicken meal (the first ingredient) from USA-raised chickens combined with our exclusive concentrate of dried vegetables and fruits. It’s ideal for dogs of all ages, sizes, and breeds.

Animal feeding tests using AAFCO procedures substantiate that Nature’s Logic Canine Chicken Meal Feast provides complete and balanced nutrition for All Life Stages.

Chicken Meal, Millet, Chicken Fat (preserved with Mixed Tocopherols), Pumpkin Seed, Yeast Culture, Spray Dried Chicken Liver, Dried Egg Product, Alfalfa Nutrient Concentrate, Montmorillonite Clay, Dried Kelp, Spray Dried Porcine Plasma, Dried Tomato, Almonds, Dried Chicory Root, Dried Carrot, Dried Apple, Menhaden Fish Meal, Dried Pumpkin, Dried Apricot, Dried Blueberry, Dried Spinach, Dried Broccoli, Dried Cranberry, Parsley, Dried Artichoke, Rosemary, Dried Mushroom, Dried Lactobacillus acidophilus Fermentation Product, Dried Lactobacillus casei Fermentation Product, Dried Bifidobacterium bifidium Fermentation Product, Dried Enterococcus faecium Fermentation Product, Dried Bacillus coagulans Fermentation Product, Dried Pineapple Extract, Dried Aspergillus niger Fermentation Extract, Dried Aspergillus oryzae Fermentation Extract, Dried Trichoderma longibrachiatum Fermentation Extract


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Banking Remittance Settlement Loans Clerk

Cheque clearance, inward/outward remittances, telegraphic transfer & demand drafts. Accept & confirm forex deals. Process & document housing loan applications.

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Tuesday, July 24, 2007

We have been experiencing the most AMAZING weather! Its hard to believe its mid-late July. On Fri it was in the upper 70s, sunny, and very low humidity. We turned the a/c off and opened the windows.

The floral arrangement I'd designed and ordered was delivered to Spouse's office and he really like it. Fri evening we met Rick & Nick (and a few other friends) for dinner. Instead of my original plan for birthday candles in individual desserts for Spouse's birthday, Rick & Nick offered to pick up a cake and secretly brought it into the restaurant when he wasn't looking. I brought glitter-y candles, which I secretly slipped to Rick under the table. When we'd finished our meal, all of the wait staff came out singing 'Happy Birthday' and placed the cake in front of Spouse. It wasn't too much of being the center of attention, which he doesn't like.

Sat morning we made our weekly pilgrimage to the Lewes farmers market, then had bagels and coffee for breakfast on the screened porch. Jordan and Pouncer enjoyed being on the screened porch with me while I read a magazine. In the afternoon Spouse & I went to the beach. It was magnificent! Then we showered, had a quick dinner at home, and headed into Rehoboth for the Follies.

Our original plan was to have dinner out and then go see 'Hairspray', but Rick & Nick gave us their complimentary tickets to the Follies since they were unable to go. The Follies is a fundraiser where local groups of friends or business associates put on skits and are judged. We attended 2 years ago and it was pretty amusing, but this year's was really bad. You'd think with a bunch of gay men involved, the singing would be great, the costumes would be fabulous and the production would be tight - but no. We decided to leave during the intermission.

It was such a gorgeous night that we walked down to the boardwalk, ran into some neighbors, and then walked back to the car. What a dreamy night for a ride with the top down. We watched a Netflix DVD when we got home, and went to bed at 1:00 am. It was great to feel the breeze from the open windows while we slept.

Sun morning we slept until 11:00 am! After a quick breakfast we began cleaning the house, then we met Rick & Nick at the beach for another magnificent day. Sun night we met up with Doug & Jim, our friends from VA who were vacationing in Rehoboth this week, for drinks at Aqua and dinner at Fusion. As usual, the downtown Rehoboth restaurant was much like the others we've tried: very expensive and mediocre food. But we had a nice time with our friends. On Tues we're going to pick them up in the convertible and ride out to Oak Orchard to eat at Serendipity, which backs to the Indian River. What a gorgeous view! And unlike all those downtown Rehoboth restaurants, Serendipity has delicious food at reasonable prices.

Drag Queen name of the day: Callie Fornia

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text:
Wednesday, June 13, 2012

The other day I was in the Dollar Tree store to pick up some chip clips.

As I made my way to the register I saw a woman pushing a shopping cart full of loaves of bread.  As she unloaded the contents of the cart onto the conveyor belt, it appeared she had 35-40 loaves of bread!

I tried to imagine why she would need/buy so many loaves of bread at one time.  Was she making sandwiches for an entire Little League team?  Was she making French Toast for a breakfast fundraiser?  I kept coming up with more and more options.

Apparently this abundance of bread was too much for the cashier to fathom.  She just had to know what was going on, so she asked the customer "Are you going to freeze most of this?"

The shopper smiled and said "No, this will all be eaten in a couple of days."  The cashier replied "Really?  You must have a houseful!", to which the customer replied "No, its for a hotel."

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User meticoore

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About: One issue that often impedes a weight loss plan is the urge to snack. It is important to plan out your snacks - just like you would plan out your meals - so that you can avoid unhealthy food when you are hungry. If you have a snack planned, then it will meet your caloric intake goal much easier for the day.

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Glow Wire Cable LED Neon party Decoration Rave 1m/3m/5m

  • $11.50
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Number of Pcs: 1pc
Brand Name: LISM
Glow Supplies Type: Other Glow Props
Model Number: LED Wire Cable
Glowing Time: Long time
Type: EL Wire Cable
Product Type: luminous ball
Characters: led party
Special Use: christmas ball led
Feature1: luminous costumes

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Feature5: glowing wires

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