text:
How Apple is changing DRM

As more stores and record labels abandon digital rights management, Apple may have an alternative plan for subscription services, writes Tim Anderson
Apple CEO Steve Jobs in front of a projection of iTunes
Apple CEO Steve Jobs launches the company's iTunes store in London in June 2004. Photograph: Ian Waldie/Getty

How Apple is changing DRM

As more stores and record labels abandon digital rights management, Apple may have an alternative plan for subscription services, writes Tim Anderson

When Apple approached record companies about selling their music digitally five years ago, they "were extremely cautious and required Apple to protect their music from being illegally copied", according to Steve Jobs's recollection of the process. That meant using digital rights management (DRM) - a software wrapper - to protect songs from unlimited copying. Jobs says it is crucial to the contract: "If our DRM system is compromised and their music becomes playable on unauthorized devices, we have only a small number of weeks to fix the problem or they can withdraw their entire music catalog from our iTunes store."

But what's the real effect of DRM? Last year, EMI began offering songs without it on iTunes. "The industry has finally been able to get some hard data about how removing DRM restrictions from legitimately purchased tracks affects piracy," says Bill Rosenplatt, DRM specialist and president of GiantSteps Media Technology Strategies. "The statistics show that there's no effect on piracy."

No effect. The assertion is remarkable. If DRM does not in fact discourage piracy, then it is merely a nuisance for the user. Now the Guardian understands that most download stores will remove DRM on permanent music downloads. "We are going to be selling non-DRM music from the summer", says Dave Elston, HMV's digital content manager, adding that it would solve "obvious interoperability issues" - primarily compatibility with Apple's iPod. Amazon has announced that its DRM-free MP3 download store, already online in the US, will be rolled out internationally later this year. Napster in the US is moving to MP3 for non-subscription downloads, and sources close to the company implied that the UK service will follow suit. And Apple offers DRM-free downloads for an increasing number of tracks.

Ironically, the music companies are now abandoning DRM because it worked too well. Apple wouldn't license its version to rivals - so the best-selling iPod drove the iTunes store to its present position, where it is the third-largest music retailer in any form in the US. Rosenblatt says that record labels "have been desperate to find a viable competitor to Apple and iTunes". Industry sources suggest that Apple's iTunes store has more than 70% of the UK download market, and growing. "The record companies don't like dealing with Apple, because Apple is in a position where it can dictate the economic terms and dictate the business models," says Rosenblatt. "What's going to draw people away from iTunes? One answer is to get rid of DRM."

Licences revoked

In the meantime, some early adopters are suffering the consequences of DRM's failure. Last month, former customers of Microsoft's defunct MSN Music store in the US received an unwelcome email. "As of August 31, 2008, we will no longer be able to support the retrieval of licence keys for the songs you purchased from MSN Music or the authorization of additional computers," it said.

So what does that mean? Protected music files are encrypted and locked with a key. To play the file, the media player must acquire a digital licence that is specific to the PC or portable device on which it is played. The licence may permit music to be copied to other computers or devices, but each device must be individually authorised by an online licence server. Without such a server, former customers of MSN Music will not be able to play their DRM-protected music on any new PCs or portable players that they buy.

The problem is worse than it first appears, since a "new" device may actually be your existing PC. Some users habitually reinstall Windows to keep it running sweetly, but doing so removes its authorisation; even adding or changing a hardware component can also break the DRM, as Microsoft notes matter-of-factly in a support article. Worse still, the DRM component in Windows can get corrupted for no apparent reason.

This is a common problem for users installing the BBC's iPlayer software, for example, which also uses Microsoft DRM. The fix, described in detail on the iPlayer support pages, involves deleting all the files in the hidden DRM folder within Windows. A side effect is that existing licences are destroyed - so existing DRM-protected files could well no longer play. In other words, there are multiple scenarios in which customers who have bought music, supposedly for a lifetime, may need to re-authorise their purchases. If the licence server has been turned off, the music will never, ever play again. What if you back up your licences? This used to be possible through Windows Media Player. But Microsoft removed the option from version 11, introduced for Windows Vista. Microsoft's Adam Anderson told us that licence backup did not work properly anyway.

Getting your backup

"The ability to back up content was not granted by every service, leading to user confusion and frustration," he says. "Third-party digital content service providers are best positioned to meet the backup and restoration needs of their customers." So the trail leads back to the licence server - which Microsoft is turning off for its customers. Why is it doing that? According to Rob Bennett, who wrote the shock email, it was too complicated to support. "Every time there is an OS upgrade, you saw support issues. People would call in because they couldn't download licences. We had to write new code, new configurations each time," he told CNet.

There are a few mitigating factors. One is that purchased downloads usually include the right to burn CDs - thus removing the DRM, and allowing proper backup. Though it's not quite perfect: most download formats are compressed. The CD will sound the same as the download - but if it is ripped back to a PC in a lossy format (such as MP3), the recompressed file will not sound as good.

Customers outside the US are not directly affected by Microsoft's move. Although several download services in the UK use Microsoft DRM - including MSN Music (which is run by Nokia), Napster and HMV - these stores have their own licence servers. Still, if Microsoft itself has done this, and if DRM for this type of purchase is on the way out, then customers with an investment in downloaded music should be cautious. Best burn them to CD, just in case. Some, however, won't let you. Subscription services such as Napster To Go, which gives temporary access to around 5m songs, will keep using DRM. But so far the subscription concept has not taken off.

Yet Rosenblatt thinks that subscriptions may turn out to be Apple's answer to the DRM-free competition - because it has already laid the groundwork with films which expire a certain length of time after being downloaded or watched. "You can now rent a movie on your iPod. The functionality on the iPod that enables that to happen is part of the functionality that you would need to support music subscription services," Rosenblatt says.

Same old problems

And that could mean the record labels will face the same problem all over again. Just as they remove DRM from their products, Apple would re-impose it. Apple customers with a subscription would probably never buy from third-party services, even while the likes of Amazon could undercut iTunes and be iPod-compatible.

Mark Mulligan, digital music analyst at Jupiter Research, also expects Apple to change its approach. "It's highly likely Apple will get into the next-generation service game. That could be Apple selling iPods preinstalled with unlimited access to music, or with a bundle to a subscription offering," he says. Mulligan sees the market evolving into multiple tiers. At the top end, a minority will be willing to pay a premium for the best quality, DRM-free downloads. The middle tier will be "subsidised offerings like Nokia's Comes With Music, where you buy a device and the cost of the music is included subsidised"; while at the bottom will be advertising-supported services such as Qtrax, SpiralFrog and We7, where free music is paid for by embedded advertising.

That would leave Apple with the top-end iTunes downloads and a subscription business. But would Jobs back it? In January 2007, Reuters asked him if Apple would do so. "Never say never, but customers don't seem to be interested in it," Jobs said. "The subscription model has failed so far." Which hardly rules it out forever. DRM might not stop pirates, but it does rivals. And in business, the latter can be a greater threat than the former.


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Crown Prince Sultan bin Abdul-Aziz obituary

Heir to the throne of Saudi Arabia, he served as defence minister
Prince Sultan bin Abdel Aziz
Crown Prince Sultan bin Abdul-Aziz created a massive military establishment, buying arms from the US, the UK and France. Photograph: Hassan Ammar/AP

Crown Prince Sultan bin Abdul-Aziz obituary

Heir to the throne of Saudi Arabia, he served as defence minister

The death of Crown Prince Sultan bin Abdul-Aziz, at the age of 80 (some sources say he was six years older), highlights the dangers of a power vacuum in Saudi Arabia, which is responsible for 25% of the world's oil. The kingdom is ruled by a frail gerontocracy, despite its oil wealth and political vulnerability. Sultan was the half-brother of the 87-year-old King Abdullah. The conservative Wahhabi kingdom has survived the Arab spring largely because Abdullah has provided $130bn (£81.5bn) in welfare aid.

As the Arab spring unwound, Sultan's role as Saudi defence and aviation minister was crucial, particularly in Bahrain, whose minority Sunni regime was supported by Saudi tanks in March. That adventure was run by Prince Nayef, the 78-year-old interior minister, who is likely to replace Sultan as crown prince.

Born in the Saudi capital, Riyadh, Sultan was the second of the elite "Sudairi seven", the sons of the kingdom's founder, King Abdul-Aziz Al Saud (commonly known as Ibn Saud) by his wife Hussa bint Ahmad al-Sudairi. Ibn Saud had some 45 recorded sons by as many as 32 wives of whom she was pre- eminent. A dominant mother, she insisted that all of her high-profile sons dine with her once a week. Fahd, the eldest, eventually became king in 1982.

Sultan received an early education in theology, modern culture and diplomacy at the royal court, where he also acquired good English. He became governor of Riyadh in 1947. In that year he oversaw the Arabian American Oil Company's (Aramco's) construction of a rail link between Dammam in the eastern province and Riyadh. He joined the cabinet in 1953, the year that Ibn Saud died and was succeeded as king by his son Saud. Sultan became agriculture minister, helping to settle Saudi Arabia's bedouin on modern farms and was considered hardworking and pugnacious. In 1955 he was appointed communications minister, a post he held until 1962.

That year Sultan became the kingdom's defence and aviation minister. When it became clear to the princes that King Saud was a failure, Sultan pressed for his abdication in favour of the charismatic Faisal, demanding that it should be brought about by force, if necessary. He stepped down in March 1964, and Faisal became king that November. Sultan was considered intelligent with innate worldly wisdom, a man on whom the king might rely.

Sultan oversaw Saudi Arabia's participation in North Yemen's bloody civil war in the 1960s which pitted monarchists against republicans supported by Nasser's Egypt. After the 1963 ceasefire the UN Swedish commander Carl von Horn believed that Sultan, "a volatile and emotional young man", had breached the ceasefire, allowing arms to cross to monarchist rebels against the wishes of King Faisal. Sultan's attempts to support the South Yemen sheikhs after Britain's withdrawal from Aden ended in fiasco when his liberation army based in Najran was cut to pieces by troops in the south inherited from the British colonial authorities.

Faisal was assassinated in 1975, and Khaled became king, with Fahd named crown prince. When Egypt signed the Camp David peace accords with Israel in 1978, Fahd and Sultan proposed continued close relations with the US, while Khaled and Abdullah, who was then a National Guard commander, advocated distancing Saudi Arabia from the US. Sultan was deeply involved in the US's covert campaign to provide money and weapons to the mujahideen fighting the Soviets in Afghanistan.

People who knew Sultan praised his "strategic vision, the capacity to think big", in particular after the 1973/74 oil price rises. Nevertheless, he had his critics. One analyst said that he presided over "the most colossal amount of money, in proportion to the size of a country's economy, ever poured down the barrel of a gun".

Sultan created a massive military establishment in Saudi Arabia through arms purchases from the US, the UK and France. He built military cities, largely with US support. However, the massive British-supported defence programme was also crucial. The 1988 al-Yamamah contract was described as Britain's largest ever export deal, worth at least £43bn, though its full extent has not been fully documented. It was later the focus of corruption charges in the UK and the US. Although Sultan was not named in these probes, members of his family were. According to David Holden in The House of Saud (1981), in the early 1970s "the majority share of transactions is said to have gone to Sultan in a ratio of about 60:40".

In 1990 US forces were deployed in Saudi Arabia to defend it against the Iraqi forces that had overrun Kuwait. His son Prince Khaled served as the top Arab commander in Operation Desert Storm, in which US, Saudi and other Arab forces drove the Iraqis out of Kuwait. In 2005, when Abdullah succeeded Fahd to the throne, Sultan became crown prince, despite rumours that he was not getting on with Abdullah.

Sultan had a reputation for a fierce temper but his habit of working deep into the night won him the nickname of "bulbul" – nightingale. He was both a conservative and political moderate. "Sultan," wrote Holden, "whose vigour on the couch was a cause for even more concern and respect, had proved a stern, tough and headstrong character."

For some time, he was said to have suffered from ill-health, having been diagnosed with colon cancer in 2004 and latterly affected by Alzheimer's disease. "Crown Prince Sultan has been for all intents and purposes incapacitated by illness for at least [the] past year," the US embassy in Riyadh wrote in a cable in May 2009, according to Wikileaks. He spent about a year abroad, recuperating in the US and at a palace in Agadir, Morocco, before returning to Saudi Arabia in November 2010, a day before King Abdullah himself went to New York for medical treatment after a blood clot complicated a slipped spinal disk.

Sultan had some 32 children by 10 wives. His son Bandar was Saudi ambassador to the US from 1983 to 2005, during which time he formed close relations with both presidents Bush.

Sultan bin Abdul-Aziz Al Saud, politician, born 30 December 1930; died 20 October 2011

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» » next post - Michael Ballé: No real lean without a sensei
« « previous post - Jon Miller: From whom do you wish to learn?
Samuel Obara

Sammy Obara: Sensei means professor

By Samuel Obara, - Last updated: Saturday, May 3, 2014 - Save & Share - Leave a comment

I am not sure what is just semantics when we differentiate a consultant from
a sensei. Is consultant a title and sensei a role? Is that a matter of
posture? In Japanese, sensei means simply professor.
I strongly believe that a sensei can be a consultant and perhaps vice versa.
In fact, some of my Toyota senseis became consultants after they retired.
Now, how good a consultant were they?

Some Toyota senseis who were very respected in Toyota and even had direct
learning from Mr. Ohno, became very poor consultants according to their
clients (“according to their clients” is the key piece of information here).
Some complaints I heard from clients were that the sensei didn’t have the
“posture” of a consultant, another one was that the sensei wasn’t sensitive
to their corporate culture, etc.

I think there must be some alignment on what to expect when hiring a sensei:

1) When senseis ask for something, we just do it, we don’t typically
challenge them. Senseis appreciate the efforts, if we do it wrongly, they
prefer to correct us later rather than to explain more before we give it a
try first.
2) When they ask a question, we have to understand it as a command. Very
often they are not explicit and they do come disguised as a question.
3) They expect us to do all (100%) of the work and keep them updated
throughout the process. For example, they could draw a few lines showing
what kind of data they are looking for, but all the collection and analysis
are done by us. Senseis will then challenge our reasoning along the way.
4) Senseis will ask questions, all the time. This makes us highly prepared
before we go sit with them.
5) One common trait I saw in many of the Toyota senseis was that they were
very patient and at the same time very intolerant. Many times they could
ask something at the end of the day and expect us to show them some results
first thing the next day. To avoid the severe consequences, we would at
least prepare something to show we gave it a try. If they saw us putting
some effort, even if not successfully, they would patiently coach us on what
to try next. If they saw we didn’t bother trying not even a little, they
could be furious to the point of sending people out of the room (and I saw
that happening more than just a couple of times).
6) Another features I thought were very common in Toyota senseis were that
many of them had a military rigor and discipline. Although they were very
rigid during the day, they were very friendly after work, and wouldn’t talk
business during happy hour. Finally the senseis I worked with, none of them
used Powerpoint or kept groups for too long in a meeting room, they were all
gemba deep. I suspect many of them don’t own a laptop.

I hope this helps organizations set their expectations so they don’t get
disappointed when they come across real senseis.r

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The Writer's Toolbox

From getting past that difficult opening line to rounding out characters, this kit will guide you round the creative obstacles on your literary path. These activities focus on a 'right-brain' creative approach to keeping productive.

  • Includes: 64 page booklet, 4 plot twist spinner pallettes, 60 creative cards, 60 wooden exercise sticks and a hinged box.
  • Box measures 15 x 22 cm.

Related Items


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Hike Gros Piton in St. Lucia

Rate this Adventure Saint Lucia Fond Gens Libre

Added by Michael Gabbert

Hike the most iconic sight of St. Lucia.

The pitons are the iconic symbols of St. Lucia. Located at the southwest part of the island, Gros Piton and Petit Piton rise dramatically out of the Caribbean Sea. At 2619 Feet, Gros Piot is both taller and far less technically challenging than its sister piton. It’s a can't-miss hike if you ever find yourself on St. Lucia.

To get to the hike, head to village of Fond Gens Libre (it's toward the southeast side of the Piton and between the towns of Soufriere and Choiseul). Once here you’ll be seeking the interpretive center. You’ll have to pay a $30 entrance fee and will be set-up with a guide for the hike as well. Note: guides are ready to go as early as 6:30/7 am and don’t require a reservation.

In total the hike is 3.2 miles with 2200 vertical feet. It starts off innocent enough with minimal elevation through mango trees. Its also quite sunny during the first quarter of the hike, but after that the hike will be under the shade of the canopy until you reach the peak.

At about the halfway point of the hike up (from a timing perspective) you run across a stunning lookout of Petite Piton. This marks the end of the “easy” portion of the hike. In fact, our guide told us that many people decide to turn around here. Fear not, while the remaining hike is quite strenuous, its doable if you’re in shape and have ever hiked before. This section is significantly more vertical with rocks, boulders and steep stairs in which you’ll be navigating all the way to the peak.

Once at the top there are two viewpoints. The first looks over the southern part of the island. The other, which is another 10 minutes across the top of the mountain gives you eye-level views of Petit Piton with the Caribbean Sea as its background. Both are well worth the short but strenuous hike you've just completed. In total the hike will take anywhere from 2.5 to 4 hrs depending on how fit you are and how quickly you're able to tackle the hike.

Expert tip: Bring a bottle or two of Piton Beer with you on the hike. There’s nothing better than drinking a Piton on the top of a Piton. Trust me.

Pack List

  • Hiking Shoes
  • Appropriate Clothing
  • Water (at least 1.5L)
  • Camera
  • Piton Beer
Read More

Please respect the places you find on The Outbound.

Always practice Leave No Trace ethics on your adventures. Be aware of local regulations and don't damage these amazing places for the sake of a photograph. Learn More

Hiking, Photography

Skill Level:

Year Round

Trail Type:

3.2 Miles

Elev. Gain:

2200 Feet

Are we missing something?

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How to Get There

Have you done this adventure? Have something to add? You could be the first to leave a review!

Added by Michael Gabbert

Minnesotan living in Seattle. Email: mjgabbert@gmail.com

More Adventures Nearby

Hike through Pigeon Island National Park

Saint Lucia / Pigeon Island NP

Pigeon Island is no longer an island as it was connected via a man-made causeway in 1972. Leave your car in the parking lot outside of the Park's gate.

Paul Kestel
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Child psychologists or child counselors in 17569. Find a therapist who specializes in children.

Search Results For Child Psychologist Near Reinholds, Pennsylvania, 17569.
Initial Search Radius: 10 Miles

Find A Therapist in 17569

Retreat at Palm Beach

Outpatient Substance Abuse & Co-Occurring Counseling
Ready for change?  Let's Get Started

1170 South State Street, Ephrata, Pennsylvania 17522

Youth Connection is an intensive program for those individuals who suffer from addiction, but are also faced with the challenges of young adulthood. Teenagers and young adults, 18 – 25 years of age, who suffer from drug or alcohol addiction often find themselves dealing with additional pressures. They may have trouble with their workload at school, associate with delinquent peers, suffer from a lack parental supervision, or just generally exhibit feelings of inadequacy, poor self-image, or even depression. They have not learned the necessary skills that would otherwise enable them to cope with everyday life situations.

Find A Therapist

Find therapy for child psychologist in Pennsylvania.


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Psychotherapist 01229. Psychotherapy that emphasizes the strength of healing and discovery.

Search Results For Psychotherapist Near Glendale, Massachusetts, 01229.
Initial Search Radius: 10 Miles

Find A Therapist in 01229

Michael MacMunn, LICSW, LADC

Licensed Social Worker, Licensed Alcohol & Drug Counselor
Ready for change?  Let's Get Started

30 Court Street, Westfield, Massachusetts 01085

I am a little different from your average therapist. I am a straight talker. I like to get right down to the issues that you bring to treatment. While we are tackling the issues, I am supportive and empathic. Another distinguishing aspect is that I am a male therapist amongst many women in the field. The other plus in seeing me is that I have over 20 years experience helping people just like you.

Siri Sokol, D.S.M. Ordained Minister

Wellness Coaching
Ready for change?  Let's Get Started

116A Dove St, Albany, New York 12210

Now, what I do as a Therapist is to myself take the role of 'dysthymic' a antagonist. In other words, I lay low. I remain objective and fairly clinical, and "outside the box" seemingly a little depressed myself so as to be on even footing with the patient or what have you. Then I track them with my associates for a long while until I am able to recognize the outcome.

Philip Kolba, MA

Ready for change?  Let's Get Started

Troy, New York 12180

I provide brief solutions-focused psychotherapy to help clients assess personal and professional functioning, clarify goals, and strengthen social and emotional skills. I specialize in brief online relationship counseling. Online counseling allows clients to seek help as needed, engage in therapy at a schedule that's convenient to them, and keep their counselor as they progress through life. I work with LGBTQ and hetero clients on individual, couple, and alternative relationship concerns. Clients explore issues that impact their professional and personal relationships, including communication, power dynamics, and intimacy. I accept US$ and cryptocurrencies.

Find A Therapist

Find therapy for psychotherapist in Massachusetts.


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Find valuable information.

Optimize your experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs.

View the relevant questions below:

Having problems with your experiment?  Visit our

Troubleshooting page

We offer three main technologies for plasmid purification: anion exchange, silica, and ChargeSwitch® chemistry.

The ratio of absorbance at 260 to the absorbance at 280 nm (A260/A280) is typically used to measure purity of the sample. For DNA, the ideal ratio is 1.8, but could be in the range 1.7–1.9. The A260/A230 ratio is also used to determine if contamination is present. The ideal ratio for DNA is 1.8–2.0. Purity of DNA can also be examined by gel analysis. For plasmid DNA, look for a strong, single band (perhaps with a few extra bands representing multimers of the desired molecule).

Anion exchange purification is recommended for higher purity and lower endotoxin levels. Silica-based purification is not optimal for transfection, as there is a higher level of endotoxins and impurities. Larger plasmids also work better with anion exchange columns.

Endotoxins are typically any cell-associated bacterial toxins that are part of the outer membrane of the cell wall of gram-negative bacteria. Endotoxins can influence cell growth, cell differentiation, contractility, and protein expression in mammalian cells. During bacterial lysis, endotoxins are released, and can reduce transfection efficiency and subsequently protein expression levels. Plasmid DNA purified with our PureLink® HiPure plasmid kits has endotoxin levels that are equivalent to endotoxin-free kits (0.1 EU/µg) for superior downstream performance. Please review the following article for more information about endotoxins: Butash KA et al. (2000) Reexamination of the effect of endotoxin on cell proliferation and transfection efficiency. Biotechniques 29(3): 610-614, 616, 618-619.

Yes, we offer our EveryPrep™ Universal Vacuum Manifold (Cat. No. K2111-01), which allows for direct elution from the manifold using our ChargeSwitch® Pro Filter Plasmid Mini, Midi, and Maxi Kits.

Here is a list of DNA/RNA Purification replacement buffers we offer as standalone.

Column-based Plasmid Purification

All kits termed “HiPure” use anion exchange resin columns to isolate the highest quality plasmid DNA, suitable for transfection. Filter kits include a filter to clear bacterial lysate without centrifugation, while the HiPure FP kits include a filter and precipitator to eliminate the need for centrifugation at either the bacterial lysate clearing or DNA precipitation step.

Yes, the PureLink® HiPure Plasmid kits can isolate BAC DNA, bacmid DNA, cosmid DNA, or M13 ssDNA. See the manual for a detailed protocol.

We would recommend using our PureLink® Quick Plasmid Kits. These preps are silica based, with fast protocols (approximately a 45 minute centrifugation). While the isolated plasmid DNA is lower in purity than DNA isolated using our PureLink® HiPure kits, it is suitable for sequencing and cloning.

HQ stands for “higher quality” silica, which provides slightly higher purity plasmid DNA than the PureLink® Quick Plasmid Kits. Endotoxin units are <40 EU/µg using the HQ silica. The plasmid DNA isolated using the PureLink® HQ kits is suitable for cloning, sequencing, and some transfection protocols.

For any silica columns, elution with water is generally possible. A buffer is preferred for stability and accuracy of absorbance readings, as pure water can have very low pH (4–5).

We do not recommend decreasing the volume of elution buffer, as this will cause yield to drop. You can try to perform an additional elution to increase yield.

Please see the chart below for our typical yields:

Prep size

Overnight bacterial culture volume

Approximate yield for high copy plasmids

1–3 mL

Up to 30 µg

25–100 mL

Up to 150 µg

100–500 mL

Up to 750 µg

0.5–2.5 L

Up to 2.5 µg

2.5–5 L

Up to 10 mg

The PureLink® HiPure Mini, Midi, and Maxi Kits can purify plasmids up to 200 kb, while the PureLink® HiPure Mega and Giga Kits can purify plasmids up to 150 kB.

Yes, please see the detailed protocol below:

  1. Equilibrate a column with 2 mL (mini)/10 mL (midi)/30 mL (maxi) of buffer EQ1.
  2. Collect the phage lysate (liquid or plate lysis) and determine the exact volume.
  3. According to the scale, add 30 μL/100 μL/400 μL of buffer X1 to 10 mL/50 mL/250 mL phage lysate and incubate at 37°C for 30 min.
    X1 = 100 mM Tris-HCl (pH 7.5), 300 mM NaCl, 10 mM EDTA, 20 mg/mL RNase A, 6 mg/mL DNase I
  4. Mix the nuclease digest from step 3 with 2 mL/ 10 mL/50 mL ice-cold buffer X2 and incubate on ice for 60 min.
    X2 = 3 M NaCl, 30% (w/v) polyethyleneglycol (PEG) 6000
  5. To collect the phage particles, centrifuge for 10 minutes at >10,000 x g. Discard the supernatant.
  6. Resuspend the pelleted phage particles in 1 mL (mini)/3 mL (midi)/9 mL (maxi) buffer X3 with a pipet.
    X3 = 100 mM Tris-HCl (pH 8.0), 25 mM EDTA
  7. Add 1 mL (mini)/3 mL (midi)/9 mL (maxi) of buffer X4 to the phage suspension. Mix thoroughly by inverting the tube several times and incubate for 10 minutes (mini) or 20 minutes (midi and maxi) at 70°C to lyse the phage particles.
    X4 = 4% (w/v) SDS
  8. Add 1 mL (mini)/3 mL (midi)/9 mL (maxi) of buffer X5 to the lysate, mix thoroughly by inverting, and centrifuge for 10 minutes at room temperature and ≥13,000 x g. Collect the supernatant without taking too many particles and apply it directly onto the equilibrated column (see step 1). Allow the lysate to enter the resin by gravity flow.
    X5 = 3.0 M potassium acetate (pH 5.5 with acetic acid)
  9. Wash the column with 2 x 2.5 mL (mini), 2 x 10 mL (midi), or 1 x 60 mL (maxi) of buffer W8.
  10. Elute the lambda DNA from the column with 0.9 mL (mini)/5 mL (midi)/15 mL (maxi) of buffer X6 and precipitate the DNA by adding 0.7 volumes of isopropanol, previously equilibrated to room temperature.
    X6 = 100 mM sodium acetate (pH 5.0 with acetic acid), 1,500 mM NaCl
  11. Centrifuge the DNA for 30 minutes at ≥13,000 x g at 4°C. Because lambda DNA is very sticky, it will spread over the whole wall of the centrifuge tube if a fixed angle rotor is used. Therefore, we recommending the use of a swinging bucket rotor (i.e. HB-4 or HB-6 for Sorvall centrifuges), or, if such a rotor is not available, using centrifuge tubes (i.e., Corex) siliconized with dimethyldichlorosilane.
    After centrifugation, was the lambda DNA with 80% ethanol and dry it briefly. Dissolve the lambda DNA in a suitable amount of TE or 10 mM Tris buffer (pH 8.0).
  1. Lysis conditions vary greatly for gram-positive bacteria, so you would need to start with a specific lysis protocol that is known to work for the particular bacteria you are working with. The lysis materials and conditions in the kit may not work well.
  2. After lysis using the specific protocol, precipitate the crude plasmid DNA with either 70% ethanol or isopropanol. Be sure that the genomic DNA is removed (follow precautions in the package insert; do not vortex at any time).
  3. Resuspend the resulting nucleic acid pellet in a small volume (1 mL for mini, 10 mL for midi, 24 mL for maxi) of 600 mM NaCl, 100 mM sodium acetate, pH 5.0.
    Apply this solution to an equilibrated column and continue with the standard protocol.

Yes, please follow our suggested protocol:

  1. 1) Lyse mammalian cells with a common mammalian lysis procedure, for instance, alkaline lysis. Please note, be careful to be gentle in this step, as sheared genomic DNA will copurify with plasmid.
  2. 2) Precipitate DNA with ethanol or 70–80% isopropanol. This is a crude precipitate.
  3. 3) Resuspend in 600 mM NaCl, 100 mM NaOAC, pH adjusted to 5.0.
  4. 4) Load onto equilibrated HiPure column.

This should work for plasmids up to 100 kb. Note that mitochondrial DNA will also copurify.

We typically recommend growing E. coli up to 1–1.5 A600 units/mL (~1 x 109 cells/mL) in LB broth.

Magnetic Bead-based Plasmid Purification

ChargeSwitch® Technology features a charged nucleic acid binding surface that is “switchable” by changing the pH of the surrounding buffer. At low pH, the surface is positively charged and binds the negatively charged nucleic acid backbone, allowing easy removal of proteins and other contaminants using a simple wash step.

Please use this link to view a table demonstrating limitations the ChargeSwitch® kits overcome over traditional plasmid purification.

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch® Pro mini kit, 100–200 µg from a 25 mL culture for the midi kit, and 500–1,000 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3–9 kb.

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (<10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

Automated Plasmid Purification

Yes, we offer the BenchPro® 2100 Plasmid Purification System, which is a fully automated maxiprep purification system.

While yield and purity will differ depending on the size of your vector and copy number as well as cell strain used, a high copy vector (pcDNA3.1) has been tested in several popular strains including C600, DH10B/T1R, DH5alpha, JM109, TOP10, and XL1-Blu, demonstrating an average yield above 500 µg.

MC5001 is lightweight and has a small footprint. This compressor includes a drying unit, which is a dual moisture trap and membrane dryer. The noise level is 60 dB. This works well in a common lab area where –80°C freezers and fume hoods are kept. The MC6001 is our ultra-quiet, oil-filled compressor. It is heavier and taller than MC5001. It also comes with the same dryer unit to reduce moisture.

For LB medium, you can fill the reservoir to the maximum volume of 150 mL. For TB medium, though we have not tested this, we have heard from customers that a 50 mL culture diluted in 50 mL medium works well.

You can pipette out 700 µL of TE, so that the final DNA concentration could be higher. We would not recommend using less than 750 µL TE buffer.

A self test is performed each time a run is started and takes approximately 6 minutes to complete.

You can use a Hedland air gauge to measure the air flow rate. The minimal specification is 3 cubic feet per minutes. There is no upper limit for flow rate.

You will need an in-house air supply outlet (80–100 psi, 3 cfm, constant flow rate), or a compressor unit with the same specifications, that can be connected to the BenchPro® 2100 Plasmid Processing Station.

No, the instrument works using positive air pressure, and cannot work on vacuum. It works using house air, which is the orange line that forces air out. The pressure has to be 80–100 psi.

You can run one or two samples simultaneously on the BenchPro® 2100 Plasmid Processing Station.

No, the BenchPro® 2100 Plasmid Purification Card is not reusable. During the purification, E. coli cells are captured and lysed within the card, making subsequent reuse impossible.

The waste tray is reusable and is made of material that can be autoclaved. You can also order new waste trays.

No. The cell liner tray and lid are not reusable. They should be disposed of after each procedure. Each BenchPro® 2100 reagent kit comes with a supply of cell liner trays and lids. Cell liner trays are not currently available for standalone purchase.

Always store the BenchPro® 2100 Plasmid Card and BenchPro® 2100 Reagent Tray in the supplied box to prevent any damage to the card sippers or to the tray foil. Store both at room temperature.

Yes, you can use an air compressor. Please contact Technical Support for recommended models. The air compressor will need to:

  • Regulate pressure output between 80 and 100 psi
  • Regulate pressure output flow at >3 cfm, when measured at 80 psi
  • Regulate output flow to <5°C dewpoint

The BenchPro® 2100 instrument needs a manual setup time of less than 5 minutes. After setup, the instrument takes about 90 minutes for the entire purification process. You do not need to be present during the purification process.

Yes. If you wish to obtain a higher concentration of plasmid DNA, you can do so by reducing the volume of elution buffer in the corresponding compartment of the reagent tray. To reduce the volume of elution buffer, use the pipette tip to pierce the foil, then draw out a corresponding volume of buffer. For specific instructions on how to do this, please contact Technical Service.

The instrument is capable of purifying plasmids from 2.7 to 20 kb.

Yes. Resuspend the cell pellets in 100 mL of LB medium before you load the mixture onto the cell liner tray.

On average, you can process 0.7 to 0.9 g of wet pellet biomass on this instrument. However, this is subject to variability when working with cells of different size and different strains.

The purified plasmid is ultrapure and suitable for all downstream applications, including those requiring the highest purity, such as transfection of mammalian cells, automated and manual DNA sequencing, PCR amplification, in vitro transcription, bacterial cell transformation, cloning, and labeling.

You can clean the surface of the BenchPro® 2100 instrument with a damp cloth. Do not use harsh reagents or solvents to clean the unit. Clean the bottom of the instrument drawer with a light spray of 70% ethanol and then wipe off the ethanol with a paper towel.

For best results, follow these guidelines:

  • Use a bacterial culture at a cell density of approximately 109 cells/mL, or an optical density of 2.0 to 2.4 at 600 nm (OD600). Use bacterial culture in transition between exponential and stationary phase.
  • Use a high copy number plasmid to obtain a good yield of plasmid DNA. High copy number plasmids typically yield 2–6 µg DNA/mL LB culture grown overnight.

We do not recommend the use of TB or rich media. A high cell density will clog the membrane and decrease DNA yield from the purification. For best results, set up the purification as indicated in the manual.

Yes. Water can be used to replace the 1.5 mL TE buffer for elution. Carefully pierce only the TE-containing chamber and remove all TE. Rinse the chamber twice with purified water and then fill with 1.5 mL purified water.

We have tested DH10B-T1R, DH5α, JM109, TOP10, and XL1-Blue in-house, and expect that any strains derived from these will also work. Note: endA+ strains have been shown to work, but there may be nuclease activity in the final purified plasmid.

No. Purification of BAC clones is not optimal due to their large size. The BenchPro®2100 instrument is capable of purifying plasmids from 2.7 to 20 kb.

Yes. You can resuspend the pellet in LB media or sterile water such that the OD at 600 nm is 2.0.

By simply using a USB device, you can download any available software updates.

After downloading the software updates, insert your USB device in the slot on the back of the instrument, and then follow instructions on the screen.

No, the current version of the BenchPro® 2100 cannot be connected to any laboratory information management systems.

No, the current version of your software will not allow you to interrupt and subsequently continue your run.


text:
Memorial Cup: Portland Winterhawks down Saskatoon Blades to clinch semifinal berth

Portland Winterhawks score three times in the third period Wednesday to defeat the Saskatoon Blades 4-2 in final round-robin game at Memorial Cup.

Saskatoon Blades' Josh Nicholls collides with Portland Winterhawks' goalie Mac Carruth during second-period action Wednesday night at the Memorial Cup in Saskatoon, Sask.
Saskatoon Blades' Josh Nicholls collides with Portland Winterhawks' goalie Mac Carruth during second-period action Wednesday night at the Memorial Cup in Saskatoon, Sask.  (TODD KOROL / REUTERS)

SASKATOON—After watching his bloodied linemate helped from the ice, Chase De Leo did all he could to make one of his best friends feel a little better — help the Portland Winterhawks make the semifinal at the MasterCard Memorial Cup.

De Leo gave Portland the lead for good in the third and the Winterhawks scored three times in the period to defeat the Saskatoon Blades 4-2 in the tournament’s final round-robin game.

With the score tied 1-1 after two, De Leo jammed a loose puck past Blades goalie Andrey Makarov at 4:08 as Portland (2-1) now moves on to Friday’s semifinal.

COX: Top prospects abound at exceptional tournament

Article Continued Below

After the game, thoughts on the Portland side quickly turned to Winterhawks right-winger Taylor Leier, who suffered what appeared to be a blow to the head on a hit from Blades defenceman Dalton Thrower just inside the Saskatoon blue-line in the first period.

A bloodied Leier, who is from Saskatoon, stayed facedown for a few minutes before being helped off the ice and did not return. The was no penalty on the play, but the Canadian Hockey League said in a release after the game that it was reviewing the incident.

“You don’t want to see a teammate go down, especially one of my best friends and linemates. It’s a scary thing,” said De Leo, who now has two goals at the tournament. “It’s not something you want to see and obviously the boys are going to get some energy and motivation to battle back for him.”

Not surprisingly, Winterhawks coach Travis Green and Blades counterpart Lorne Molleken had different interpretations of the hit.

“It’s pretty obvious where the shot comes. It hits him in the head and there is a jump to it as well. The video is pretty obvious,” Green said. “We lost one of our better players to a hit that I thought should have been a major.”

Molleken, meanwhile, called the hit “unfortunate” but added that he thought Thrower caught Leier in the chest with his shoulder.

Winterhawks players disagreed.

“When you see a teammate go down like that you’re not going to stand by and watch but at this point in the tournament and how big of a game it was, we can’t take any stupid penalties,” said Portland forward Ty Rattie, who scored his fourth goal. “(Leier) got his head hit. It was a hit to the head.”

The host Blades (1-2), who were swept out of the Western Hockey League playoffs in the first round and have never won a Memorial Cup, now have a tough task with three games in four nights.

A win on Wednesday would have meant a bye to the final, but Saskatoon now faces a Thursday tiebreaker game against the Ontario Hockey League champion London Knights (1-2).

“Going through adversity is nothing new for this team. We’ve had our ups and downs this year and we’re looking forward to proving ourselves again and taking the long way around,” said Saskatoon overage forward Josh Nicholls, who had a goal and an assist. “This a typical Blades way of doing things — taking that long way. Hopefully we get the job done and take three straight.”

The winner of the Saskatoon-London game will meet Portland in Friday’s semi. With the Winterhawks’ victory, the Halifax Mooseheads (2-1) clinched a berth directly into Sunday’s final to decide the CHL champion.

Derrick Pouliot and Brenden Leipsic had the other goals for Portland (2-1), which got 29 saves from Mac Carruth. Nicolas Petan added two assists for the WHL champions.

Shane McColgan also scored for Saskatoon, while Makarov stopped 30 shots in taking the loss.

“It was a big win,” Green said. “I thought their team came out hard like we expected early, tried to be physical on us and we withheld and I thought we slowly took over the game.”

After De Leo scored to give Portland the lead in the third, Leipsic added an insurance goal for Portland at 6:27, ripping a shot from the slot into the top corner past Makarov.

Rattie made it 4-1 with his fourth of the tournament at 10:16, beating Makarov to the glove side off the rush.

“We knew it was going to be hard and to get three quick bingos like that was big for the boys,” Rattie said. “(It) gave us a little bit more confidence and a little bit more leeway on defence.”

Nicholls scored at 16:27 to cut the deficit to two, but the Blades would get no closer.

“Portland did a good job against us in a lot of areas but these teams are all skilled teams and when we get into that game it’s going to be difficult on us so we have to play a simplified game,” Molleken said. “We have to play a north game and make sure our forecheck is good but tonight we let Carruth handle far too many pucks.”

Following a scoreless first period, the teams traded goals in the second. Pouliot opened the scoring at 6:44 after jumping on a Blades turnover and firing a shot from the sideboards that fooled Makarov through the five-hole.

Saskatoon got a 5-on-3 power play later in the period and didn’t generate much of anything until McColgan, who hit the post moments earlier, fired a shot past Carruth that deflected in off a Winterhawks player in front with a second left on the second penalty to tie the score at 11:20.

Portland had a golden opportunity to retake the lead when Blades left-winger Michael Ferland took a double minor for high sticking with just over four minutes remaining in the period. Winterhawks defenceman Seth Jones, the No. 1 ranked North American skater according to NHL Central Scouting ahead of June’s draft, had a good chance off the rush but fired wide as the Blades weathered the storm and were saluted off the ice by a standing ovation from the crowd of 9,239 at the Credit Union Centre.

Coming off Sunday’s emotional 5-2 victory over Halifax, the Blades said prior to the game that their focus would be to get on the body early and they did just that, hitting the Winterhawks at every opportunity in the first period — including Thrower’s crushing blow on Leier.

The Blades, who have had their share of doubters this season, don’t have much time to refocus ahead of Thursday’s tiebreaker.

“Things are just going to increase in tempo here. It all counts now because lose and you’re done. There is no tomorrow anymore so we’ve really got to strap up and put our work boots on,” defenceman Duncan Siemens. “We’re a hard-nosed team. We have some skill up front but the majority of team is meat and potatoes. We’ve got to go to those hard areas and score those greasy goals.”

Notes: The Winterhawks have won two Memorial Cups (1983, 1998). The Blades have never won a CHL title. . . . Former NHL tough guy and Blades alumni Joe Kocur was in attendance.


text:
Honeymoon in Brazil

Honeymoon in Brazil Honeymoon in Brazil

Brazil. Where life can be a carnival, or a party on a world famous beach, or the best start ever.

Brazil will present you with a store of shared memories that will last for years to come. You could, for example, take a boat to the foot of the dazzling Iguazu Falls and gaze in awe at the soaring cascade of crashing water. But you’re on honeymoon, so you might hire yourself a helicopter and fly over the Falls for a real once-in-a-lifetime experience.

You’ll also want to take full advantage of private excursions to Corcovado, and to the iconic Sugar Loaf Mountain, its monumental presence towering over Rio de Janeiro - one of the most vivacious cities in the whole of South America.

And then there is strolling along the world famous Copacabana Beach at sunset. An absolute must-do on your perfect honeymoon to Brazil.

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Schedule 1 — The Child Poverty Commission

Part of Bill Presented — Fiscal Responsibility – in the House of Commons at 6:41 pm on 9th December 2009.

Alert me about debates like this

Photo of Graham Stuart Graham Stuart Conservative, Beverley and Holderness 6:41 pm, 9th December 2009

Thank you, Madam Deputy Speaker. Of course, it will be under the auspices of this Bill that any future Conservative Government would have to address child poverty-it will be the lens through which they look at it-so talking about what they would do is what the debate on this Bill is about. Unless it is repealed, it will determine and set a framework in which future Governments will have to deal with this important issue.

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Propaganda Vs Persuasion

Misuse of Language and Meaning

Hoarding Treasure Troves
Vasiliki Varvaki/Moment Mobile/Getty Images

When most people think of propaganda, they tend to think of the posters and songs created by or with the aid of a government during wartime, yet the truth of the matter is that propaganda has a much broader application. It refers not only to efforts by a government to get people to adopt certain beliefs or attitudes, but it can also be applied to the ways in which corporations try to get you to buy things.

What is it?

What is propaganda? Broadly speaking, we can label as “propaganda” any organized effort to persuade large numbers of people about the truth of an idea, the value of a product, or the appropriateness of an attitude. Propaganda isn’t a form of communication which simply seeks to inform; instead, it is both directional (because it often seeks to get people to act in some fashion) and emotional (because it seeks to condition certain emotional reactions to specific situations).

When the government uses the media in an organized and deliberate way to get people to believe that a war is necessary for their safety, that’s propaganda. When a corporation uses the media in an organized and deliberate way to get people to think that a new type of razor is better than the old, that’s propaganda. Finally, if a private group uses the media in an organized and deliberate way to get people to adopt a negative attitude towards immigrants, that’s also propaganda.

One might ask what the difference is between propaganda and arguments in general — after all, isn’t an argument designed to establish the truth of a proposition and thus, at least implicitly, get people to accept the truth of that proposition? The key difference here is that while an argument is designed to establish the truth of a proposition, propaganda is designed to spread the adoption of an idea, regardless of its truth and always in a one-sided manner.

Please keep in mind, though, that simply labeling something as “propaganda” does not automatically say anything at all about the truth, value, or appropriateness of what is being “sold.” Using the above examples, perhaps it is true that the war is necessary, the new razor is better, and people shouldn’t have a positive attitude towards immigrants. There is nothing about “propaganda” which requires that it be used for false or misleading purposes. Examples of propaganda tools being used for good might be the large-scale programs to discourage drunk-driving or to convince people to register to vote.

So why is there a general perception that propaganda is bad? Because propaganda is concerned with spreading the adoption of an idea regardless of its truth, people are much more likely to look upon it skeptically. Despite the fact that most people don’t do a great job at critical thinking, they do still care about the truth and think that others should as well. If they believe that some organization is pushing an agenda without regard for the truth, they’re going to have a negative reaction.

In addition, we must keep in mind that propaganda is used for misleading purposes quite a lot.

It is so common for propaganda to commit fallacies, engage in distortion, and be filled with lots of other errors that it is very difficult to imagine propaganda ever not being that way. As a matter of fact, propaganda often works best when we fail to reason about the message very carefully. In today’s world we are all bombarded with so many messages and so much information that it is tempting to take mental shortcuts in order to process it all in any way. Yet mental shortcuts that bypass critical reasoning are exactly those which allow propagandistic messages to influence our beliefs and attitudes without our realizing it.

Still, because the connection is automatic, we can’t imagine that labeling something as propaganda therefore says anything about conclusions it offers. Moreover, because the term “propaganda” is an emotionally loaded label, no critique of propaganda should start with that label.

Instead, it is better to first provide a critique and then, after the arguments are refuted or dismantled, point out that it qualified as a form of propaganda.


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Sinchon Santa Village

Things to do, Conventions
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Sinchon Santa Village

What is it?

A mass blind date organized by a company called Saemip where singles will go to be matched up with other singles to dine together at several designated foodie spots.

Who’s going?

Saemip will be taking applications for 600 single women and 600 single men. (Ages 20-35 only). Non-Koreans are welcome to join but applications in Korean only

Early registration: 32,000 won for men and 29,000 won for women

A word from the organizer

“In Korea, they sometimes say we’re the ‘sam-po-si-dae’ meaning we’ve given up on courtship, marriage and having kids. I think that our program gives hope to people that just think there isn’t an opportunity for them anymore. I’ve heard a lot of happy stories from our event. Survey shows over 80% satisfaction. People on our staff get to participate at one of the events for free, so it’s nice to hear when they get matched up, too. Two of the participants from last year are even getting married!”

Where to get more info (Korean only).

By: Hahna Yoon

Event website:
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Share to learn

August 25, 2016

Interdisciplinary cooperation is one of the values that Bath Spa University prides itself on, so I was delighted to read Nick Jennings of Imperial College London advocating this approach (“Overly specialised graduates risk replacement by machines”, News, 18 August).

Collaborations between subject areas mean that students are exposed to creative practitioners and researchers. One of the most iconic examples of creative collaboration is Apple. When introducing the iPad 2 in 2012, Steve Jobs said that “technology alone is not enough – it’s technology married with liberal arts, married with humanities, that yields results that make our hearts sing”.

Curricula that encourage entrepreneurial thought allow students to enter successful careers within pioneering companies or start their own businesses, adding further diversity to the UK economy.

It is encouraging that more universities and academics from a range of subject backgrounds are starting to see the benefits of interdisciplinarity as a way to, in Jennings’ words, “tackle grander global challenges”. If the STEM sector is looking for inspiration on the best way to do this, they could look to the creative arts, which have long thrived on a diet of shared knowledge and expertise. With the UK creative industries worth £84.1 billion a year to the UK economy, it is clear this approach is working.

Christina Slade
Bath Spa University

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Pioneering the study of inhumanity

The story of a remarkable research centre devoted to understanding “the roots of extremism” has been reconstructed in a new radio documentary

March 16, 2014

Source: Shutterstock

Warning sign at Auschwitz concentration camp, Poland

The centre’s origins stem from a speech by the then Observer editor David Astor in April 1962, in which he argued that since most Nazi leaders and supporters were “not mad in a medical sense”, we had to confront something “deeply alarming and disturbing” about human nature - namely “the pathological possibilities of the normal mind”.

This led him to call for a centre to study what he called “political psycho-pathology”.

Mr Astor was approached by the historian Norman Cohn, who had worked as an army intelligence officer in occupied Austria and was preoccupied by similar themes.

After discussions, he later recalled, “David Astor made me an offer such as can seldom have been made to any scholar: he gave me an undertaking that if I would resign the professorship I then held in the University of Durham and devote myself to implementing the suggestion contained in his address, he would see to it that I would not suffer financially.”

After research convinced him that “the official killings of alleged witches in the 16th and 17th centuries” offered the best parallel to the Holocaust, Professor Cohn began work on what became two major books: Warrant for Genocide, The Myth of the Jewish World Conspiracy and the Protocols of the Elders of Zion and Europe’s Inner Demons: An Enquiry Inspired by the Great Witch-Hunt.

From 1966 to 1980, he also served as the one and only director of what became the Columbus Centre at the University of Sussex.

Others scholars went on to examine “the authoritarian personality”, leadership, ideology and the way groups stereotype outsiders, and applied such insights to the persecution of the gypsies and the “psycho-dynamics” of racial prejudice.

Professor Cohn’s PhD student Albie Sachs produced a major study on Justice in South Africa for the centre.

“Fathoming the enigma of that all too human thing, inhumanity, became a great quest of the post-war years,” explains Daniel Pick, professor of history at Birkbeck, University of London, in the radio documentary The Roots of Extremism.

Through interviews with the main participants, their children and some of today’s leading experts in the darker aspects of ourselves, he reveals the important role the Columbus Centre played in that “quest”.

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Cramming study into the shortest possible time will impoverish the student experience and drive an even greater wedge between research-enabled permanent staff and the growing underclass of flexible teaching staff, says Tom Cutterham


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by marcos arthur ( BR ) 2011-08-31 19:33:42 GMT

muita boa qualidade, perfeito, ótimo desenho,arredondado bom acabamento. ponto negativo : estão vindo com bateria descarregada, este é o terceiro que compro e vem com bateria pifada.

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by marcosj10 ( BR ) 2013-09-19 17:53:29 GMT
Involvement: Geral (Sabe como usar o produto) Ownership: Menos de uma semana

Pros: Relógio show de bola! Esse é o terceiro relógio do mesmo modelo da Curren que eu tenho (tenho caixa dourada e preta e um todo prata)e esse é o mais bonito dos três! Numeros bem detalhados, desenho interno impecável! Tenho varios modelos de relógios da Curren e esse é um dos três mais bonitos entre todos os modelos.

Sobre o envio...Muito bom tb protegeram bem o relógio com plastico bolha e ele chegou aqui intacto!

Cons: Nem é um problemão...Mas as baterias já chegam quase que no fim da vida util. (deve ser pq o relógio é um modelo mais antigo, 2010)

Bottomline: Recomendo a quem esteja com interesse de comprar o relógio a concluir a compra! Vale muito a pena!

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Wishing all our customers, partners and suppliers Seasons Greetings! It has been a pleasure working with you throughout the year. We wish you a very healthy and prosperous 2017 and look forward to working with you again in the New Year.

Order & Delivery during Christmas Closure

Our office will be closed from the 24th of December to the 2nd of January. Please note that we will be unable to despatch any goods before Tuesday 6th. Contact our service to arrange any new order as soon as possible. Apologies for any inconvenience and have a wonderful Christmas Time!

Merry Christmas Everybody!And Happy New Year


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Contact Us

Tiny Green PC is headquartered in London. We provide sales and support across Europe via our technical team based in the UK (London, Camden Town or Mornington Crescent) and our office in Italy (Bari). Whether you're looking to place or track an order, make a return, change delivery details, resolve an issue, or any general enquiries; you can do all this by completing our contact form below or talking to us on our live chat facility at the bottom of the screen.

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Tiny Green PC
(trading as Anders Electronics PLC)
Kings Studios
43-45 Kings Terrace
London, NW1 0JR

Tel:+44(0)800 0098006

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Via Marconi 93
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It is second time when I ordered hardware from TinyGreenPc and as at first time - it is excellent. Server is very fast - I migrated my NAS, LAN router, development server and wifi AP to it. All on one machine."

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4509 Tolt Ave, Carnation, WA
(425) 333-4066
  • Camp Tolt Knit Night

    This past Friday we had our first Camp Tolt fireside knit night.  We had a great time in the Pacific Northwest twilight. Lots of wonderful knitters came from near and far to spend the evening with us at Tolt McDonald Park, a 500 acre campground right here in Carnation, WA.

    Rachel knitting her Bandera Kerchief in Cestari Ash Lawn (75% Virginia Cotton, 25% US Wool)

     We have two other Camp Tolt Knit Nights scheduled for July 22nd and August 19th, at which Linda Rostad will do a special talk about her knitting and mountaineering adventures. Linda is an avid knitter and has been backpacking in the Pacific NW for over 50 years, including completing the entire Pacific Crest Trail over time.

    We hope to see you around the campfire with us and out adventuring with your knits on your own!

    Knit for adventure!

Added to cart


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Rare Opportunity with Garden Near Bacvice Beach in Split

By , 26 Jun 2015, 08:31 AM Real Estate

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  • Location: Split
  • Price: 400,000 €
  • Bedrooms: 3

Looking to be close to Split's iconic Bacvice beach?

Looking for an older-style property with a garden in the Bacvice area? They are few and far between on the market, but our friends from Trgostan have one. In their own words:

A beautiful three bedroom apartment of app 120 sqm, on the ground floor of an older villa in Bacvice area, in one of the most sought-after locations, fantastic maintained garden of app 100 sqm, possible to make a parking spot, rarely on the market!

Indoor space 120m2. Outdoor space 100m2. Price 400,000 euro. For more information and to view, click here.

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Croatia Traffic Info

  • Due to a traffic accident traffic is flowing with difficulties on Zagreb detour road (A3) from Jankomir junction towards Lučko junction. Due to strong wind at Dubrovnik bridge traffic is suspended for: doubledeckers, trailers and motorcycles. Wind is blowing also on the A6 Rijeka-Zagreb motorway from Kikovica to Tuhobić tunnel and on the A1 motorway from Božići viaduct to Sveti Rok tunnel (driving speed limit of 40 km/h). During the day difficulties can be expected in roadwork areas. Until 31 March at 5pm traffic is suspended on Rijeka detour road (A7) at Rujevica junction. Drivers are invited to keep distance and to adjust the speed to road conditions. With the bad weather the headlights have to be on during the day as well. One lane is free only/traffic is flowing in reduced lanes on the following motorways:-A2 Zagreb-Macelj between Zaprešić and Zabok junctions 20th-18th km in both directions; -A3 Bregana-Lipovac between Kutina and Novska junctions 106th-108th km, 118th-121st km in both directions; -A4 Zagreb-Goričan in Hrastovec tunnel in both directions; -A6 Rijeka-Zagreb at Svilno viaduct 78th-80th km between Orehovica and Čavle junctions in each direction till 1 June; -A8 in Istria on the section Lupoglav-Učka tunnel. Roadworks on the A4 Zagreb-Goričan motorway (Zagreb detour road): - till 9 June traffic is suspended in the entrance lane of Kraljevečki Novaki from direction Sesvete and Dugo Selo towards Ivanja Reka junction, there is a local detour; in the roadwork area traffic is flowing in reduced lanes; - till 26 April traffic is suspended in the exit lane of Popovec junction from direction Goričan towards Popovec, detour for direction Vrbovec-Popovec: Sveta Helena junction (A4)-Sveta Helena-DC3 Goričica-Belovar-Žerjavinec-Soblinec-Popovec junction, for direction Goričan (A4)-Sveta Helena: Komin junction (A4)-DC3-Sveti Ivan Zelina-Donja Zelina-Žerjavinec-Soblinec-Popovec. Lepenica rest area (at 57th km) on the A6 Rijeka-Zagreb motorway is closed till 15 June, the previous rest area and petrol station is the one Ravna Gora (at 30th km), the next one Cernik-Čavle (17,8 km away from Lepenica). Due to roadworks traffic is suspended: -on the DC1 state road in Lučko and Stupnik (Zagreb); -on the DC8 Adriatic road at Posedarje till 10 April; -on the DC6 state road, section Glina-Dvor border crossing, detour: Glina (DC37)-Petrinja-Hrvatska Kostajnica-Dvor; -on the DC25 Lički Osik-Karlobag state road (at 40th km) 8am-3pm till 24 March. Traffic is regulated by traffic signals/one road lane is free only:-on the DC1 state road at Tušilović and Mostanje; -on the DC1 Sinj-Klis Grlo state road at Dicmo; -on the DC8 Adriatic road at Poličnik, on the section Kaštel Stari-Kaštel Gomilica, at Podstrana, on the section Zaton Doli-Bistrina, at Srebreno; -on the DC66 Pula-Most Raša state road; -on the DC75 Poreč detour road, section Vrvari-Bijela; -on the DC62 Veliki Prolog-Metković state road at Most Metković. 15 November 2016-15 April 2017 winter equipment is mandatory on the winter sections of the state roads in Croatia (it includes four winter tyres or four summer tyres with chains ready for use).
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  • In the outbound freight traffic there are delays at Bregana (the queue is 800 m), Rupa (2 km) and Pasjak. Due to roadworks at Županja and Slavonski Brod border crossings delays can be expected. At Harmica border crossing traffic is allowed for vehicles up to 7,5 tonnes only.
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  • Ubli-Vela Luka-Hvar-Split catamaran does not operate. Jelsa-Bol-Split catamaran does not stop at Bol and Jelsa ports. Ferries operate according to timetable.
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US Diagnostics

US Diagnostics provides excellent diabetic management care at an affordable cost. US Diagnostics develops, manufactures and distributes blood glucose monitoring systems. The primary focus of the company is to develop technologies in the areas of biosensor and dry reagent chemistry that enable rapid point of care testing of the blood.

8 Items
$ 4.99 $ 6.86

2 in stock!

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Added to Shopping Bag

Robert Clergerie Black Flats

Robert Clergerie Black Flats

Listed by wendy k.

Size: 10 Regular (M, B)

Price: $180.00

Returnable for Site Credit You can request a return for Tradesy Site Credit within 4 days of delivery. FREE return shipping.

If your item was misrepresented in any way, you can file a misrepresentation claim. Tradesy will investigate and you’ll be eligible for a Full Refund.

Shopping Bag :

Black Flats

Size: 10  Regular (M, B) Item #: 3500944
Price: $180.00

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7 people saved this item

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This item may have been worn but has no visible signs of wear.

Robert Clergerie black leather lace up loafers. These go amazing well with pants suits. Believe it or not, these shoes are light as a feather and extremely comfortable.

Regular (M, B)

Estimated U.S. Delivery

Sat 4/1/17 - Tue 4/4/17 (5-8 days)

Fair & Simple Returns

Feel free to return this item for any reason and get Tradesy Credit. Use your credit to buy something that makes you happy. Just a heads-up, purchases outside the U.S. are Final Sale.

  1. 1.

    Submit a return request within 4 days of delivery

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    Pack and ship the item using our free return shipping label

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    Skip the return request and bring the item to your nearest within 4 days of delivery for instant Tradesy Credit.

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Tradesy only allows the sale of authentic designer goods. If you believe your purchase is a replica, send it to our team for a free evaluation. You’ll get a full refund if anything’s amiss.*

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Get what you ordered, or your money back. We’ll give you a full refund if the condition, style, or authenticity of an item are misrepresented in any way.*

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22 Items
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Employment Opportunities

Transfer Flow is looking for a Marketing Coordinator, a new key member to its growing marketing team. The idea candidate will be driven, reliable and versatile. This role includes a wide variety of responsibilities covering the conception, execution, measurement and refinement of myriad marketing campaign covering all media and several customer segments. You’ll help shape email and online campaigns, print and online content, and a variety of efforts aimed at increasing sales through our various distribution channels. You should be very adept at crafting and conveying a compelling value proposition for each distinct customer type and be driven to optimize through well-conceived experiments and data analysis. Market research, customer segmentation, competitive analysis and sales promotion will be other areas of focus. You should have a degree and/or 3-5 years of experience reaching customers to drive sales through various media, ideally for a company that sold hard goods. Business writing experience and talent is a must, and proficiency with basic math, data analysis and spreadsheet use is important. Automotive industry, digital marketing, and social marketing experience is a plus but not necessarily required, depending on other training or relevant experience.

Transfer Flow is the leading manufacturer of fuel tank systems for both aftermarket use and OEM customers. Proudly patriotic and committed to growing our business on the founding principles of high-quality U.S. manufacturing, fair business practices and exceptional customer service, Transfer Flow believes it’s critical to grow our team based not only on skill set but also the character of individuals that will ideally match that of our existing team. Family owned and values driven for more than 33 years, Transfer Flow is among the area’s best employers offering terrific benefits and a great working environment, as evidenced by our employees’ incredible average years of service, which exceeds 10 years. Growing steadily, Transfer Flow just launched a second manufacturing shift and has in excess of 100 valued employees.

Please email a resume, writing samples and a cover letter explaining relevant marketing experience to markf@transferflow.com. We are unable to respond to applicants arriving at our facility without a scheduled appointment.

Marketing Coordinator is a permanent, full-time non-exempt position.

DOE, Medical, Dental, Vision, Holidays, Vacation, 401k


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Photo: Because India.

India forum

Indian visa in Bangkok?

Posted by miriam123 on 19/12/2013 at 00:48

Hi I am going to apply for a Indian visa in Bangkok next week to leave near the end of January / early February. I have not booked a flight yet has anyone got a visa without a flight or do I need one booked to get the visa. Also I wasn't going to book a return as I don't know the date or airport I will fly back toBangkok with. I have a fight from Bangkok to Dubai in early March, will this flight be enough to show that I am leaving India?

#1 miriam123 has been a member since 19/7/2013. Posts: 28
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Bar Revenge

5-7 Marine Parade, Brighton, United Kingdom

| website | facebook | +44 1273 608 133 |

audience rating Bar Revenge, 3.0 out of 5 based on 1 rating
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GD Star Rating

Pre-party music bar playing chart, house and R&B (with the occasional throwback night where its all 90’s). Bar Revenge offers a good selection of draught and bottled beer, cocktails and wine.

Discounted entry passes to Club Revenge, but the bar does stay open all night on Fridays and Saturdays.

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venue bar

features bar, music, cabaret show

exclusively gay

weekday Mon-Wed 12:00 - 01:00; Thu 12:00 - 02:00

weekend 12:00 - 06:00 Sun 12:00 - 01:00

last updated: 02-03-2017

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Torture Gardens

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audience rating
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The Torture Garden, the experts in fetish club nights, hold regular kinky parties in Edinburgh. Located at The Caves are regular ‘anything goes’ hardcore parties for people to explore their sexuality and hook up with like-minded fetish folks. Not all the parties hosted by Torture Gardens are directed towards the gay crowds, but obviously there are man-on-man events for you to explore your fetish fantasies. Dress appropriately and abide by the rules. See the website for details of particular events to coincide with your visit.

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venue cruise / fetish

features bar, music, dancing, dark room, adults-only, cruise / fetish

customers Mixed

attracts All types

last updated: 11-10-2015

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The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this.


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Suite (Score & Parts)-BSN/BASS

Composer: Montano, Damian

Publisher: TrevCo

Edition: 5404

Suite for bassoon and double bass

I. Prelude: Moderato

II. Marziale

III. Menuet, Grazioso

IV. Barcarolle, Largo

V. Gigue, Vivace

There are two versions of this piece. The original version is for Bassoon and Double Bass.  The composer then made a transcription for Bassoon and Contrabassoon. Although the bassoon part is basically the same, there are differences in the Bass and Contra parts due to the unique abilities, design, and qualities of those two instruments.


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The latest reviews. The lowest prices. The perfect place to shop for hotels.

Maria Angola Hotel

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Victoria, Canada
Reviewed 3 weeks ago

It starts when you enter the elevator, what a mess. and when you get to your room, leave your socks on.. the carpet and bathroom are super dirty. The breakfast starts at 7, however, all the food is not put out until 7:30ish.

Thank KL-Traveller-2012
Bexhill-on-Sea, United Kingdom
Reviewed 19 September 2016 via mobile

We stayed here for one night and would never consider staying here again. The negative points were that the carpet was disgustingly dirty so much so that it made our feet brown. Also, the sheets had a lot of hair on them and stains, extremely disappointing as you would expect them to put new sheets on after the last people...

1Thank Peter F
The Hague, The Netherlands
Reviewed 28 June 2016 via mobile

noisey: windows aren't soundproof. cleaning staff listening to music on their phone's loudspeakers. could hear other guests talk. mold in bathroom. dirty carpet. dirty walls. water stains. no proper power suplies. lights flicker when using power general bad interior. bed was okay didn't eat in restaurant. front desk staff weren't helpfull when i asked for a quiet room, eventually got...

1Thank vonschinken
Reviewed 15 May 2016 via mobile

Stayed 5 days. If you stay facing the street will be difficult to sleep. The rooms smell bad, the bathroom worst. Looks unattended, half of the lights works. Keep forgetting to replace towels. For the price go for something better. The location is ok

1Thank Jose B
Northeastern Ontario, Canada
Reviewed 24 March 2016 via mobile

This hotel has clearly been improving things all over . My experience this year was much better than the last. The hotel is cleaner , the breakfast has slightly improved( there is a toaster at the buffet, so you çan toast the fresh buns and croissants from the table), the wireless Internet service has greatly improved. And as always the...

Thank Ben B
Oahu, Hawaii
Reviewed 10 March 2016 via mobile

Since I was attending a conference at the convention center I booked a stay at this hotel. I should've here's the advice of other reviewers. The rooms are inexpensive and there's several reasons why. It is very run down and it doesn't appear to be going through any type of renovations. There were cigarette burns in my carpet, bobby pins...

1Thank HawaiiBorn69
SW Florida
Reviewed 28 February 2016

Stayed for 2 nights, traveled to Cusco and environs for 4 nights, and now have returned for another week for a meeting at the convention center across the street. Here with a colleague. This is indeed a run down hotel. Would love to have hooks in the bathroom, but my biggest complaint is that the hot water is unpredictable. On...

Thank No_nameSWFlorida
Reviewed 20 November 2015 via mobile

Super Noisy, you get here doors closing in the hallways very easily. The wifi is spotty and constantly requires you to log in and add your password again and again. The hotel lacks maintenance. The staff was ok??. Location is decent however.

Thank Peter A
Reviewed 13 November 2015 via mobile

Truly the best experience. The room and our accommodations were superb. The staff was helpful and our meal was delicious. The location was excellent and unlike other countries our communication with staff was excellent. Our beds were comfortable and transportation from airport was easy. Truly an experience to remember.

Thank Y3946AYdavidm
Reviewed 10 October 2015 via mobile

I stayed there for 3 nights. Well located but it clearly lacked maintainance. Staff seemed distracted and incapable to provise muxh assistance. Wifi connection was extremely poor. In an executive room there was no desk (and no wifi). Breakfast was simple. All in all: unless they do not decide to upgrade and invest a bit, go for a better option

1Thank Davbcn

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  • Free High Speed Internet ( WiFi )
  • Restaurant
  • Bar/Lounge
  • Breakfast included
About the property
  • Wheelchair Access
Things to do
  • Restaurant
  • Bar/Lounge
  • Free Internet
  • Free High Speed Internet ( WiFi )
  • Breakfast included
  • Meeting Rooms
  • Business Centre with Internet Access
  • Laundry Service
  • Banquet Room
  • Multilingual Staff
  • Conference Facilities
Official Description (provided by the hotel)
Contemporary Commercial Hotel situated in Miraflores, the financial & commercial district. ... more   less

Additional Information about Maria Angola Hotel

Address: Av. La Paz 610 | Miraflores, Lima, Peru
Region: Peru > Lima Region > Lima > Miraflores
Price Range: $82 - $114 (Based on Average Rates for a Standard Room)
Hotel Class:3 star — Maria Angola Hotel 3*
Number of rooms: 84
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TripAdvisor is proud to partner with Booking.com, Travelocity, Agoda and Hotels.com so you can book your Maria Angola Hotel reservations with confidence. We help millions of travellers each month to find the perfect hotel for both holiday and business trips, always with the best discounts and special offers.
Also Known As:
Maria Angola Lima
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Typical questions asked:
  • Can tickets for local attractions be purchased at the front desk?
  • Do you have to pay extra for a mini-fridge?
  • At what time does the pool open each day?

See which rooms travellers prefer

“Do not get a room facing the street.”
 of 5 bubbles Barbados_Lover101, 10 May 2015 | Read review
“if you get a bad room, be very nice to them, tell them whats wrong with your room. if they like you, you get a really nice room, if they dont, there are no rooms available.”
  • one of their nicer rooms
  • a view of the street from the nifty balconies
  • the big black spot on the carpet was the offensive odor in the room, described as a combination
 of 5 bubbles W3933SPbenb, 20 Feb 2015 | Read review
“Ask to see the room before you accept it.”
 of 5 bubbles vicdavid, 2 Jan 2015 | Read review
“did not use fitness or swimming.. not sure if they have these facilities.”
 of 5 bubbles patricios2014, 12 Jun 2014 | Read review
“its noisy, dirty, smells, service is unreal.. awfull”
 of 5 bubbles RenataB928, 23 Dec 2012 | Read review
5 of 5


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From Review: They treated us well... of Fairfield Inn & Suites Beloit


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Seafood Restaurants in Waldo

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Randy's Rib Shack

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Matched on: American Seafood Barbecue