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Academic Appointments

Honors & Awards

  • K99/R00 Pathway to Independence Award, NIH/NCI (2015 - Pres)
  • Visionary Postdoctoral Fellowship, Dept. of Defense (2012 - 2015)
  • NIH T32 Cancer Biology Training Grant, Stanford University (2012)

Boards, Advisory Committees, Professional Organizations

  • Member, International Society for Computational Biology (ISCB) (2008 - Present)
  • Associate Member, American Association for Cancer Research (AACR) (2012 - Present)

Professional Education

  • PhD, University of California, Santa Barbara, Biomolecular Science and Engineering Program (2010)

Research & Scholarship

Current Research and Scholarly Interests

Our group combines computational and experimental techniques to study the cellular organization of complex tissues, with a focus on determining the phenotypic diversity and clinical significance of tumor cell subsets. Although our research cuts across disciplinary boundaries, we specialize in the development of robust computational strategies to address key questions in the cancer genomics field, with an emphasis on clinical translation of our findings into novel biomarkers and individualized therapies.

As a member of the Department of Biomedical Data Science and the Institute for Stem Cell Biology and Regenerative Medicine, and as an affiliate of graduate programs in Biomedical Informatics, Cancer Biology, and Immunology, we are also interested in the development of impactful biomedical data science tools in areas beyond our immediate research focus, including developmental biology, regenerative medicine, and systems immunology.

2017-18 Courses

Stanford Advisees

Graduate and Fellowship Programs

All Publications

  • Integrated digital error suppression for improved detection of circulating tumor DNA NATURE BIOTECHNOLOGY Newman, A. M., Lovejoy, A. F., Klass, D. M., Kurtz, D. M., Chabon, J. J., Scherer, F., Stehr, H., Liu, C., Bratman, S. V., Say, C., Zhou, L., Carter, J. N., West, R. B., Sledge Jr, G. W., Shrager, J. B., Loo Jr, B. W., Neal, J. W., Wakelee, H. A., Diehn, M., Alizadeh, A. A. 2016

    View details for DOI 10.1038/nbt.3520

  • Robust enumeration of cell subsets from tissue expression profiles NATURE METHODS Newman, A. M., Liu, C., Green, M. R., Gentles, A. J., Feng, W., Xu, Y., Hoang, C. D., Diehn, M., Alizadeh, A. A. 2015

    View details for DOI 10.1038/nmeth.3337

  • The prognostic landscape of genes and infiltrating immune cells across human cancers NATURE MEDICINE Gentles, A. J., Newman, A. M. (co-first author) , Liu, C., Bratman, S. V., Feng, W., Nair, V. S., Xu, Y., Khuong, A., Hoang, C. D., Diehn, M., West, R. B., Plevritis, S. K., Alizadeh, A. A. 2015

    View details for DOI 10.1038/nm.3909

  • An ultrasensitive method for quantitating circulating tumor DNA with broad patient coverage NATURE MEDICINE Newman, A. M., Bratman, S. V., To, J., Wynne, J. F., Eclov, N. C., Modlin, L. A., Liu, C. L., Neal, J. W., Wakelee, H. A., Merritt, R. E., Shrager, J. B., Loo, B. W., Alizadeh, A. A., Diehn, M. 2014; 20 (5): 552-558

    Circulating tumor DNA (ctDNA) is a promising biomarker for noninvasive assessment of cancer burden, but existing ctDNA detection methods have insufficient sensitivity or patient coverage for broad clinical applicability. Here we introduce cancer personalized profiling by deep sequencing (CAPP-Seq), an economical and ultrasensitive method for quantifying ctDNA. We implemented CAPP-Seq for non-small-cell lung cancer (NSCLC) with a design covering multiple classes of somatic alterations that identified mutations in >95% of tumors. We detected ctDNA in 100% of patients with stage II-IV NSCLC and in 50% of patients with stage I, with 96% specificity for mutant allele fractions down to ∼0.02%. Levels of ctDNA were highly correlated with tumor volume and distinguished between residual disease and treatment-related imaging changes, and measurement of ctDNA levels allowed for earlier response assessment than radiographic approaches. Finally, we evaluated biopsy-free tumor screening and genotyping with CAPP-Seq. We envision that CAPP-Seq could be routinely applied clinically to detect and monitor diverse malignancies, thus facilitating personalized cancer therapy.

    View details for DOI 10.1038/nm.3519

    View details for Web of Science ID 000335710700028

  • FACTERA: a practical method for the discovery of genomic rearrangements at breakpoint resolution BIOINFORMATICS Newman, A. M., Bratman, S. V., Stehr, H., Lee, L. J., Liu, C., Diehn, M., Alizadeh, A. A. 2014
  • Identification of a colonial chordate histocompatibility gene SCIENCE Voskoboynik, A., Newman, A. M. (co-first author) , Corey, D. M., Sahoo, D., Pushkarev, D., et al 2013; 341 (6144): 384-387

    View details for DOI 10.1126/science.1238036

  • Lab-Specific Gene Expression Signatures in Pluripotent Stem Cells CELL STEM CELL Newman, A. M., Cooper, J. B. 2010; 7 (2): 258-262

    Pluripotent stem cells derived from both embryonic and reprogrammed somatic cells have significant potential for human regenerative medicine. Despite similarities in developmental potential, however, several groups have found fundamental differences between embryonic stem cell (ESC) and induced-pluripotent stem cell (iPSC) lines that may have important implications for iPSC-based medical therapies. Using an unsupervised clustering algorithm, we further studied the genetic homogeneity of iPSC and ESC lines by reanalyzing microarray gene expression data from seven different laboratories. Unexpectedly, this analysis revealed a strong correlation between gene expression signatures and specific laboratories in both ESC and iPSC lines. Nearly one-third of the genes with lab-specific expression signatures are also differentially expressed between ESCs and iPSCs. These data are consistent with the hypothesis that in vitro microenvironmental context differentially impacts the gene expression signatures of both iPSCs and ESCs.

    View details for DOI 10.1016/j.stem.2010.06.016

    View details for Web of Science ID 000281107400017

    View details for PubMedID 20682451

  • Profiling Tumor Infiltrating Immune Cells with CIBERSORT. Methods in molecular biology (Clifton, N.J.) Chen, B., Khodadoust, M. S., Liu, C. L., Newman, A. M., Alizadeh, A. A. 2018; 1711: 243–59

    Tumor infiltrating leukocytes (TILs) are an integral component of the tumor microenvironment and have been found to correlate with prognosis and response to therapy. Methods to enumerate immune subsets such as immunohistochemistry or flow cytometry suffer from limitations in phenotypic markers and can be challenging to practically implement and standardize. An alternative approach is to acquire aggregative high dimensional data from cellular mixtures and to subsequently infer the cellular components computationally. We recently described CIBERSORT, a versatile computational method for quantifying cell fractions from bulk tissue gene expression profiles (GEPs). Combining support vector regression with prior knowledge of expression profiles from purified leukocyte subsets, CIBERSORT can accurately estimate the immune composition of a tumor biopsy. In this chapter, we provide a primer on the CIBERSORT method and illustrate its use for characterizing TILs in tumor samples profiled by microarray or RNA-Seq.

    View details for DOI 10.1007/978-1-4939-7493-1_12

    View details for PubMedID 29344893

  • Genomic feature selection by coverage design optimization Journal of Applied Statistics Reid, S., Newman, A. M., Diehn, M., Alizadeh, A. A., Tibshirani, R. 2018
  • Early B cell changes predict autoimmunity following combination immune checkpoint blockade. The Journal of clinical investigation Das, R., Bar, N., Ferreira, M., Newman, A. M., Zhang, L., Bailur, J. K., Bacchiocchi, A., Kluger, H., Wei, W., Halaban, R., Sznol, M., Dhodapkar, M. V., Dhodapkar, K. M. 2018

    Combination checkpoint blockade (CCB) targeting inhibitory CTLA4 and PD1 receptors holds promise for cancer therapy. Immune-related adverse events (IRAEs) remain a major obstacle for the optimal application of CCB in cancer. Here, we analyzed B cell changes in patients with melanoma following treatment with either anti-CTLA4 or anti-PD1, or in combination. CCB therapy led to changes in circulating B cells that were detectable after the first cycle of therapy and characterized by a decline in circulating B cells and an increase in CD21lo B cells and plasmablasts. PD1 expression was higher in the CD21lo B cells, and B cell receptor sequencing of these cells demonstrated greater clonality and a higher frequency of clones compared with CD21hi cells. CCB induced proliferation in the CD21lo compartment, and single-cell RNA sequencing identified B cell activation in cells with genomic profiles of CD21lo B cells in vivo. Increased clonality of circulating B cells following CCB occurred in some patients. Treatment-induced changes in B cells preceded and correlated with both the frequency and timing of IRAEs. Patients with early B cell changes experienced higher rates of grade 3 or higher IRAEs 6 months after CCB. Thus, early changes in B cells following CCB may identify patients who are at increased risk of IRAEs, and preemptive strategies targeting B cells may reduce toxicities in these patients.

    View details for DOI 10.1172/JCI96798

    View details for PubMedID 29309048

  • Combination approach for detecting different types of alterations in circulating tumor DNA in leiomyosarcoma. Clinical cancer research : an official journal of the American Association for Cancer Research Przybyl, J., Chabon, J. J., Spans, L., Ganjoo, K., Vennam, S., Newman, A. M., Forgó, E., Varma, S., Zhu, S., Debiec-Rychter, M., Alizadeh, A. A., Diehn, M., van de Rijn, M. 2018

    The clinical utility of circulating tumor DNA (ctDNA) monitoring has been shown in tumors that harbor highly recurrent mutations. Leiomyosarcoma (LMS) represents a type of tumor with a wide spectrum of heterogeneous genomic abnormalities; thus, targeting hotspot mutations or a narrow genomic region for ctDNA detection may not be practical. Here we demonstrate a combinatorial approach that integrates different sequencing protocols for the orthogonal detection of single nucleotide variants (SNVs), small indels and copy number alterations (CNAs) in ctDNA.We employed Cancer Personalized Profiling by deep Sequencing (CAPP-Seq) for the analysis of SNVs and indels, together with a genome-wide interrogation of CNAs by Genome Representation Profiling (GRP). We profiled 28 longitudinal plasma samples and 25 tumor specimens from 7 patients with LMS.We detected ctDNA in 6 of 7 of these patients with >98% specificity for mutant allele fractions down to a level of 0.01%. We show that results from CAPP-Seq and GRP are highly concordant, and the combination of these methods allows for more comprehensive monitoring of ctDNA by profiling a wide spectrum of tumor-specific markers. By analyzing multiple tumor specimens in individual patients obtained from different sites and at different times during treatment, we observed clonal evolution of these tumors that was reflected by ctDNA profiles.Our strategy allows for a comprehensive monitoring of a broad spectrum of tumor-specific markers in plasma. Our approach may be clinically useful not only in LMS but also in other tumor types that lack recurrent genomic alterations.

    View details for DOI 10.1158/1078-0432.CCR-17-3704

    View details for PubMedID 29463554

  • Macrophage infiltration and genetic landscape of undifferentiated uterine sarcomas. JCI insight Przybyl, J., Kowalewska, M., Quattrone, A., Dewaele, B., Vanspauwen, V., Varma, S., Vennam, S., Newman, A. M., Swierniak, M., Bakula-Zalewska, E., Siedlecki, J. A., Bidzinski, M., Cools, J., van de Rijn, M., Debiec-Rychter, M. 2017; 2 (11)

    Endometrial stromal tumors include translocation-associated low- and high-grade endometrial stromal sarcomas (ESS) and highly malignant undifferentiated uterine sarcomas (UUS). UUS is considered a poorly defined group of aggressive tumors and is often seen as a diagnosis of exclusion after ESS and leiomyosarcoma (LMS) have been ruled out. We performed a comprehensive analysis of gene expression, copy number variation, point mutations, and immune cell infiltrates in the largest series to date of all major types of uterine sarcomas to shed light on the biology of UUS and to identify potential novel therapeutic targets. We show that UUS tumors have a distinct molecular profile from LMS and ESS. Gene expression and immunohistochemical analyses revealed the presence of high numbers of tumor-associated macrophages (TAMs) in UUS, which makes UUS patients suitable candidates for therapies targeting TAMs. Our results show a high genomic instability of UUS and downregulation of several TP53-mediated tumor suppressor genes, such as NDN, CDH11, and NDRG4. Moreover, we demonstrate that UUS carry somatic mutations in several oncogenes and tumor suppressor genes implicated in RAS/PI3K/AKT/mTOR, ERBB3, and Hedgehog signaling.

    View details for DOI 10.1172/jci.insight.94033

    View details for PubMedID 28570276

  • Antigen presentation profiling reveals recognition of lymphoma immunoglobulin neoantigens NATURE Khodadoust, M. S., Olsson, N., Wagar, L. E., Haabeth, O. A., Chen, B., Swaminathan, K., Rawson, K., Liu, C. L., Steiner, D., Lund, P., Rao, S., Zhang, L., Marceau, C., Stehr, H., Newman, A. M., Czerwinski, D. K., Carlton, V. E., Moorhead, M., Faham, M., Kohrt, H. E., Carette, J., Green, M. R., Davis, M. M., Levy, R., Elias, J. E., Alizadeh, A. A. 2017; 543 (7647): 723-?

    Cancer somatic mutations can generate neoantigens that distinguish malignant from normal cells. However, the personalized identification and validation of neoantigens remains a major challenge. Here we discover neoantigens in human mantle-cell lymphomas by using an integrated genomic and proteomic strategy that interrogates tumour antigen peptides presented by major histocompatibility complex (MHC) class I and class II molecules. We applied this approach to systematically characterize MHC ligands from 17 patients. Remarkably, all discovered neoantigenic peptides were exclusively derived from the lymphoma immunoglobulin heavy- or light-chain variable regions. Although we identified MHC presentation of private polymorphic germline alleles, no mutated peptides were recovered from non-immunoglobulin somatically mutated genes. Somatic mutations within the immunoglobulin variable region were almost exclusively presented by MHC class II. We isolated circulating CD4(+) T cells specific for immunoglobulin-derived neoantigens and found these cells could mediate killing of autologous lymphoma cells. These results demonstrate that an integrative approach combining MHC isolation, peptide identification, and exome sequencing is an effective platform to uncover tumour neoantigens. Application of this strategy to human lymphoma implicates immunoglobulin neoantigens as targets for lymphoma immunotherapy.

    View details for DOI 10.1038/nature21433

    View details for Web of Science ID 000397619700057

    View details for PubMedID 28329770

  • Early detection of molecular residual disease in localized lung cancer by circulating tumor DNA profiling. Cancer discovery Chaudhuri, A. A., Chabon, J. J., Lovejoy, A. F., Newman, A. M., Stehr, H., Azad, T. D., Khodadoust, M. S., Esfahani, M. S., Liu, C. L., Zhou, L., Scherer, F., Kurtz, D. M., Say, C., Carter, J. N., Merriott, D. J., Dudley, J. C., Binkley, M. S., Modlin, L., Padda, S. K., Gensheimer, M. F., West, R. B., Shrager, J. B., Neal, J. W., Wakelee, H. A., Loo, B. W., Alizadeh, A. A., Diehn, M. 2017

    Identifying molecular residual disease (MRD) after treatment of localized lung cancer could facilitate early intervention and personalization of adjuvant therapies. Here we apply Cancer Personalized Profiling by Deep Sequencing (CAPP-Seq) circulating tumor DNA (ctDNA) analysis to 255 samples from 40 patients treated with curative intent for stage I-III lung cancer and 54 healthy adults. In 94% of evaluable patients experiencing recurrence, ctDNA was detectable in the first post-treatment blood sample, indicating reliable identification of MRD. Post-treatment ctDNA detection preceded radiographic progression in 72% of patients by a median of 5.2 months and 53% of patients harbored ctDNA mutation profiles associated with favorable responses to tyrosine kinase inhibitors or immune checkpoint blockade. Collectively, these results indicate that ctDNA MRD in lung cancer patients can be accurately detected using CAPP-Seq and may allow personalized adjuvant treatment while disease burden is lowest.

    View details for DOI 10.1158/2159-8290.CD-17-0716

    View details for PubMedID 28899864

  • Targeted chromatin ligation, a robust epigenetic profiling technique for small cell numbers. Nucleic acids research Zarnegar, M. A., Reinitz, F., Newman, A. M., Clarke, M. F. 2017; 45 (17): e153

    The complexity and inefficiency of chromatin immunoprecipitation strategies restrict their sensitivity and application when examining rare cell populations. We developed a new technique that replaces immunoprecipitation with a simplified chromatin fragmentation and proximity ligation step that eliminates bead purification and washing steps. We present a simple single tube proximity ligation technique, targeted chromatin ligation, that captures histone modification patterns with only 200 cells. Our technique eliminates loss of material and sensitivity due to multiple inefficient steps, while simplifying the workflow to enhance sensitivity and create the potential for novel applications.

    View details for DOI 10.1093/nar/gkx648

    View details for PubMedID 28973448

    View details for PubMedCentralID PMC5622369

  • Data normalization considerations for digital tumor dissection. Genome biology Newman, A. M., Gentles, A. J., Liu, C. L., Diehn, M., Alizadeh, A. A. 2017; 18 (1): 128

    In a recently published article in Genome Biology, Li and colleagues introduced TIMER, a gene expression deconvolution approach for studying tumor-infiltrating leukocytes (TILs) in 23 cancer types profiled by The Cancer Genome Atlas. Methods to characterize TIL biology are increasingly important, and the authors offer several arguments in favor of their strategy. Several of these claims warrant further discussion and highlight the critical importance of data normalization in gene expression deconvolution applications.Please see related Li et al correspondence: and Zheng correspondence:

    View details for DOI 10.1186/s13059-017-1257-4

    View details for PubMedID 28679399

  • Distinct biological subtypes and patterns of genome evolution in lymphoma revealed by circulating tumor DNA SCIENCE TRANSLATIONAL MEDICINE Scherer, F., Kurtz, D. M., Newman, A. M., Stehr, H., Craig, A. F., Esfahani, M. S., Lovejoy, A. F., Chabon, J. J., Klass, D. M., Liu, C. L., Zhou, L., Glover, C., Visser, B. C., Poultsides, G. A., Advani, R. H., Maeda, L. S., Gupta, N. K., Levy, R., Ohgami, R. S., Kunder, C. A., Diehn, M., Alizadeh, A. A. 2016; 8 (364)

    Patients with diffuse large B cell lymphoma (DLBCL) exhibit marked diversity in tumor behavior and outcomes, yet the identification of poor-risk groups remains challenging. In addition, the biology underlying these differences is incompletely understood. We hypothesized that characterization of mutational heterogeneity and genomic evolution using circulating tumor DNA (ctDNA) profiling could reveal molecular determinants of adverse outcomes. To address this hypothesis, we applied cancer personalized profiling by deep sequencing (CAPP-Seq) analysis to tumor biopsies and cell-free DNA samples from 92 lymphoma patients and 24 healthy subjects. At diagnosis, the amount of ctDNA was found to strongly correlate with clinical indices and was independently predictive of patient outcomes. We demonstrate that ctDNA genotyping can classify transcriptionally defined tumor subtypes, including DLBCL cell of origin, directly from plasma. By simultaneously tracking multiple somatic mutations in ctDNA, our approach outperformed immunoglobulin sequencing and radiographic imaging for the detection of minimal residual disease and facilitated noninvasive identification of emergent resistance mutations to targeted therapies. In addition, we identified distinct patterns of clonal evolution distinguishing indolent follicular lymphomas from those that transformed into DLBCL, allowing for potential noninvasive prediction of histological transformation. Collectively, our results demonstrate that ctDNA analysis reveals biological factors that underlie lymphoma clinical outcomes and could facilitate individualized therapy.

    View details for DOI 10.1126/scitranslmed.aai8545

    View details for Web of Science ID 000389448100006

    View details for PubMedID 27831904

  • Role of KEAP1/NRF2 and TP53 Mutations in Lung Squamous Cell Carcinoma Development and Radiation Resistance. Cancer discovery Jeong, Y., Hoang, N. T., Lovejoy, A., Stehr, H., Newman, A. M., Gentles, A. J., Kong, W., Truong, D., Martin, S., Chaudhuri, A., Heiser, D., Zhou, L., Say, C., Carter, J. N., Hiniker, S. M., Loo, B. W., West, R. B., Beachy, P., Alizadeh, A. A., Diehn, M. 2016

    Lung squamous cell carcinoma (LSCC) pathogenesis remains incompletely understood, and biomarkers predicting treatment response remain lacking. Here, we describe novel murine LSCC models driven by loss of Trp53 and Keap1, both of which are frequently mutated in human LSCCs. Homozygous inactivation of Keap1 or Trp53 promoted airway basal stem cell (ABSC) self-renewal, suggesting that mutations in these genes lead to expansion of mutant stem cell clones. Deletion of Trp53 and Keap1 in ABSCs, but not more differentiated tracheal cells, produced tumors recapitulating histologic and molecular features of human LSCCs, indicating that they represent the likely cell of origin in this model. Deletion of Keap1 promoted tumor aggressiveness, metastasis, and resistance to oxidative stress and radiotherapy (RT). KEAP1/NRF2 mutation status predicted risk of local recurrence after RT in patients with non-small lung cancer (NSCLC) and could be noninvasively identified in circulating tumor DNA. Thus, KEAP1/NRF2 mutations could serve as predictive biomarkers for personalization of therapeutic strategies for NSCLCs.We developed an LSCC mouse model involving Trp53 and Keap1, which are frequently mutated in human LSCCs. In this model, ABSCs are the cell of origin of these tumors. KEAP1/NRF2 mutations increase radioresistance and predict local tumor recurrence in radiotherapy patients. Our findings are of potential clinical relevance and could lead to personalized treatment strategies for tumors with KEAP1/NRF2 mutations. Cancer Discov; 7(1); 86-101. ©2016 AACR.This article is highlighted in the In This Issue feature, p. 1.

    View details for PubMedID 27663899

    View details for PubMedCentralID PMC5222718

  • High-throughput genomic profiling of tumor-infiltrating leukocytes. Current opinion in immunology Newman, A. M., Alizadeh, A. A. 2016; 41: 77-84

    Tumors are complex ecosystems comprised of diverse cell types including malignant cells, mesenchymal cells, and tumor-infiltrating leukocytes (TILs). While TILs are well known to play important roles in many aspects of cancer biology, recent developments in immuno-oncology have spurred considerable interest in TILs, particularly in relation to their optimal engagement by emerging immunotherapies. Traditionally, the enumeration of TIL phenotypic diversity and composition in solid tumors has relied on resolving single cells by flow cytometry and immunohistochemical methods. However, advances in genome-wide technologies and computational methods are now allowing TILs to be profiled with increasingly high resolution and accuracy directly from RNA mixtures of bulk tumor samples. In this review, we highlight recent progress in the development of in silico tumor dissection methods, and illustrate examples of how these strategies can be applied to characterize TILs in human tumors to facilitate personalized cancer therapy.

    View details for DOI 10.1016/j.coi.2016.06.006

    View details for PubMedID 27372732

  • Circulating tumour DNA profiling reveals heterogeneity of EGFR inhibitor resistance mechanisms in lung cancer patients NATURE COMMUNICATIONS Chabon, J. J., Simmons, A. D., Lovejoy, A. F., Esfahani, M. S., Newman, A. M., Haringsma, H. J., Kurtz, D. M., Stehr, H., Scherer, F., Karlovich, C. A., Harding, T. C., Durkin, K. A., Otterson, G. A., Purcell, W. T., Camidge, D. R., Goldman, J. W., Sequist, L. V., Piotrowska, Z., Wakelee, H. A., Neal, J. W., Alizadeh, A. A., Diehn, M. 2016; 7

    Circulating tumour DNA (ctDNA) analysis facilitates studies of tumour heterogeneity. Here we employ CAPP-Seq ctDNA analysis to study resistance mechanisms in 43 non-small cell lung cancer (NSCLC) patients treated with the third-generation epidermal growth factor receptor (EGFR) inhibitor rociletinib. We observe multiple resistance mechanisms in 46% of patients after treatment with first-line inhibitors, indicating frequent intra-patient heterogeneity. Rociletinib resistance recurrently involves MET, EGFR, PIK3CA, ERRB2, KRAS and RB1. We describe a novel EGFR L798I mutation and find that EGFR C797S, which arises in ∼33% of patients after osimertinib treatment, occurs in <3% after rociletinib. Increased MET copy number is the most frequent rociletinib resistance mechanism in this cohort and patients with multiple pre-existing mechanisms (T790M and MET) experience inferior responses. Similarly, rociletinib-resistant xenografts develop MET amplification that can be overcome with the MET inhibitor crizotinib. These results underscore the importance of tumour heterogeneity in NSCLC and the utility of ctDNA-based resistance mechanism assessment.

    View details for DOI 10.1038/ncomms11815

    View details for Web of Science ID 000378007200001

    View details for PubMedID 27283993

    View details for PubMedCentralID PMC4906406

  • Identification of tumorigenic cells and therapeutic targets in pancreatic neuroendocrine tumors PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Krampitz, G. W., George, B. M., Willingham, S. B., Volkmer, J., Weiskopf, K., Jahchan, N., Newman, A. M., Sahoo, D., Zemek, A. J., Yanovsky, R. L., Nguyen, J. K., Schnorr, P. J., Mazur, P. K., Sage, J., Longacre, T. A., Visser, B. C., Poultsides, G. A., Norton, J. A., Weissman, I. L. 2016; 113 (16): 4464-4469

    Pancreatic neuroendocrine tumors (PanNETs) are a type of pancreatic cancer with limited therapeutic options. Consequently, most patients with advanced disease die from tumor progression. Current evidence indicates that a subset of cancer cells is responsible for tumor development, metastasis, and recurrence, and targeting these tumor-initiating cells is necessary to eradicate tumors. However, tumor-initiating cells and the biological processes that promote pathogenesis remain largely uncharacterized in PanNETs. Here we profile primary and metastatic tumors from an index patient and demonstrate that MET proto-oncogene activation is important for tumor growth in PanNET xenograft models. We identify a highly tumorigenic cell population within several independent surgically acquired PanNETs characterized by increased cell-surface protein CD90 expression and aldehyde dehydrogenase A1 (ALDHA1) activity, and provide in vitro and in vivo evidence for their stem-like properties. We performed proteomic profiling of 332 antigens in two cell lines and four primary tumors, and showed that CD47, a cell-surface protein that acts as a "don't eat me" signal co-opted by cancers to evade innate immune surveillance, is ubiquitously expressed. Moreover, CD47 coexpresses with MET and is enriched in CD90(hi)cells. Furthermore, blocking CD47 signaling promotes engulfment of tumor cells by macrophages in vitro and inhibits xenograft tumor growth, prevents metastases, and prolongs survival in vivo.

    View details for DOI 10.1073/pnas.1600007113

    View details for Web of Science ID 000374393800063

    View details for PubMedID 27035983

    View details for PubMedCentralID PMC4843455

  • Skin fibrosis. Identification and isolation of a dermal lineage with intrinsic fibrogenic potential. Science Rinkevich, Y., Walmsley, G. G., Hu, M. S., Maan, Z. N., Newman, A. M., Drukker, M., Januszyk, M., Krampitz, G. W., Gurtner, G. C., Lorenz, H. P., Weissman, I. L., Longaker, M. T. 2015; 348 (6232)

    Dermal fibroblasts represent a heterogeneous population of cells with diverse features that remain largely undefined. We reveal the presence of at least two fibroblast lineages in murine dorsal skin. Lineage tracing and transplantation assays demonstrate that a single fibroblast lineage is responsible for the bulk of connective tissue deposition during embryonic development, cutaneous wound healing, radiation fibrosis, and cancer stroma formation. Lineage-specific cell ablation leads to diminished connective tissue deposition in wounds and reduces melanoma growth. Using flow cytometry, we identify CD26/DPP4 as a surface marker that allows isolation of this lineage. Small molecule-based inhibition of CD26/DPP4 enzymatic activity during wound healing results in diminished cutaneous scarring. Identification and isolation of these lineages hold promise for translational medicine aimed at in vivo modulation of fibrogenic behavior.

    View details for DOI 10.1126/science.aaa2151

    View details for PubMedID 25883361

    View details for PubMedCentralID PMC5088503

  • Large-Scale and Comprehensive Immune Profiling and Functional Analysis of Normal Human Aging. PloS one Whiting, C. C., Siebert, J., Newman, A. M., Du, H., Alizadeh, A. A., Goronzy, J., Weyand, C. M., Krishnan, E., Fathman, C. G., Maecker, H. T. 2015; 10 (7)

    While many age-associated immune changes have been reported, a comprehensive set of metrics of immune aging is lacking. Here we report data from 243 healthy adults aged 40-97, for whom we measured clinical and functional parameters, serum cytokines, cytokines and gene expression in stimulated and unstimulated PBMC, PBMC phenotypes, and cytokine-stimulated pSTAT signaling in whole blood. Although highly heterogeneous across individuals, many of these assays revealed trends by age, sex, and CMV status, to greater or lesser degrees. Age, then sex and CMV status, showed the greatest impact on the immune system, as measured by the percentage of assay readouts with significant differences. An elastic net regression model could optimally predict age with 14 analytes from different assays. This reinforces the importance of multivariate analysis for defining a healthy immune system. These data provide a reference for others measuring immune parameters in older people.

    View details for DOI 10.1371/journal.pone.0133627

    View details for PubMedID 26197454

  • Potential clinical utility of ultrasensitive circulating tumor DNA detection with CAPP-Seq. Expert review of molecular diagnostics Bratman, S. V., Newman, A. M., Alizadeh, A. A., Diehn, M. 2015: 1–5

    Tumors continually shed DNA into the circulation, where it can be noninvasively accessed. The ability to accurately detect circulating tumor DNA (ctDNA) could significantly impact the management of patients with nearly every cancer type. Quantitation of ctDNA could allow objective response assessment, detection of minimal residual disease and noninvasive tumor genotyping. The latter application overcomes the barriers currently limiting repeated tumor tissue sampling during therapy. Recent technical advancements have improved upon the sensitivity, specificity and feasibility of ctDNA detection and promise to enable innovative clinical applications. Here, we focus on the potential clinical utility of ctDNA analysis using CAncer Personalized Profiling by deep Sequencing (CAPP-Seq), a novel next-generation sequencing-based approach for ultrasensitive ctDNA detection. Applications of CAPP-Seq for the personalization of cancer detection and therapy are discussed.

    View details for DOI 10.1586/14737159.2015.1019476

    View details for PubMedID 25773944

  • In Vivo clonal analysis reveals lineage-restricted progenitor characteristics in Mammalian kidney development, maintenance, and regeneration. Cell reports Rinkevich, Y., Montoro, D. T., Contreras-Trujillo, H., Harari-Steinberg, O., Newman, A. M., Tsai, J. M., Lim, X., Van-Amerongen, R., Bowman, A., Januszyk, M., Pleniceanu, O., Nusse, R., Longaker, M. T., Weissman, I. L., Dekel, B. 2014; 7 (4): 1270-1283

    The mechanism and magnitude by which the mammalian kidney generates and maintains its proximal tubules, distal tubules, and collecting ducts remain controversial. Here, we use long-term in vivo genetic lineage tracing and clonal analysis of individual cells from kidneys undergoing development, maintenance, and regeneration. We show that the adult mammalian kidney undergoes continuous tubulogenesis via expansions of fate-restricted clones. Kidneys recovering from damage undergo tubulogenesis through expansions of clones with segment-specific borders, and renal spheres developing in vitro from individual cells maintain distinct, segment-specific fates. Analysis of mice derived by transfer of color-marked embryonic stem cells (ESCs) into uncolored blastocysts demonstrates that nephrons are polyclonal, developing from expansions of singly fated clones. Finally, we show that adult renal clones are derived from Wnt-responsive precursors, and their tracing in vivo generates tubules that are segment specific. Collectively, these analyses demonstrate that fate-restricted precursors functioning as unipotent progenitors continuously maintain and self-preserve the mouse kidney throughout life.

    View details for DOI 10.1016/j.celrep.2014.04.018

    View details for PubMedID 24835991

    View details for PubMedCentralID PMC4425291

  • Efficient Selection of Biomineralizing DNA Aptamers Using Deep Sequencing and Population Clustering ACS Nano Bawazer, L. A., Newman, A. M. (co-first author) , Gu, Q., Ibish, A., Arcila, M., Cooper, J. B., Meldrum, F., Morse, D. E. 2014; 8 (1): 387-395

    View details for DOI 10.1021/nn404448s

  • Identifying Stem Cell Gene Expression Patterns and Phenotypic Networks with AutoSOME. Methods in molecular biology (Clifton, N.J.) Newman, A. M., Cooper, J. B. 2014; 1150: 115-130

    Stem cells have the unique property of differentiation and self-renewal and play critical roles in normal development, tissue repair, and disease. To promote systems-wide analysis of cells and tissues, we developed AutoSOME, a machine-learning method for identifying coordinated gene expression patterns and correlated cellular phenotypes in whole-transcriptome data, without prior knowledge of cluster number or structure. Here, we present a facile primer demonstrating the use of AutoSOME for identification and characterization of stem cell gene expression signatures and for visualization of transcriptome networks using Cytoscape. This protocol should serve as a general foundation for gene expression cluster analysis of stem cells, with applications for studying pluripotency, multi-lineage potential, and neoplastic disease.

    View details for DOI 10.1007/978-1-4939-0512-6_6

    View details for PubMedID 24743993

  • The genome sequence of the colonial chordate, Botryllus schlosseri. eLife Voskoboynik, A., Neff, N. F., Sahoo, D., Newman, A. M., Pushkarev, D., Koh, W., Passarelli, B., Fan, H. C., Mantalas, G. L., Palmeri, K. J., Ishizuka, K. J., Gissi, C., Griggio, F., Ben-Shlomo, R., Corey, D. M., Penland, L., White, R. A., Weissman, I. L., Quake, S. R. 2013; 2

    Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confidently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration. DOI:

    View details for DOI 10.7554/eLife.00569

    View details for PubMedID 23840927

  • Systems-level analysis of age-related macular degeneration reveals global biomarkers and phenotype-specific functional networks GENOME MEDICINE Newman, A. M., Gallo, N. B., Hancox, L. S., Miller, N. J., Radeke, C. M., Maloney, M. A., Cooper, J. B., Hageman, G. S., Anderson, D. H., Johnson, L. V., Radeke, M. J. 2012; 4

    Please see related commentary: macular degeneration (AMD) is a leading cause of blindness that affects the central region of the retinal pigmented epithelium (RPE), choroid, and neural retina. Initially characterized by an accumulation of sub-RPE deposits, AMD leads to progressive retinal degeneration, and in advanced cases, irreversible vision loss. Although genetic analysis, animal models, and cell culture systems have yielded important insights into AMD, the molecular pathways underlying AMD's onset and progression remain poorly delineated. We sought to better understand the molecular underpinnings of this devastating disease by performing the first comparative transcriptome analysis of AMD and normal human donor eyes.RPE-choroid and retina tissue samples were obtained from a common cohort of 31 normal, 26 AMD, and 11 potential pre-AMD human donor eyes. Transcriptome profiles were generated for macular and extramacular regions, and statistical and bioinformatic methods were employed to identify disease-associated gene signatures and functionally enriched protein association networks. Selected genes of high significance were validated using an independent donor cohort.We identified over 50 annotated genes enriched in cell-mediated immune responses that are globally over-expressed in RPE-choroid AMD phenotypes. Using a machine learning model and a second donor cohort, we show that the top 20 global genes are predictive of AMD clinical diagnosis. We also discovered functionally enriched gene sets in the RPE-choroid that delineate the advanced AMD phenotypes, neovascular AMD and geographic atrophy. Moreover, we identified a graded increase of transcript levels in the retina related to wound response, complement cascade, and neurogenesis that strongly correlates with decreased levels of phototransduction transcripts and increased AMD severity. Based on our findings, we assembled protein-protein interactomes that highlight functional networks likely to be involved in AMD pathogenesis.We discovered new global biomarkers and gene expression signatures of AMD. These results are consistent with a model whereby cell-based inflammatory responses represent a central feature of AMD etiology, and depending on genetics, environment, or stochastic factors, may give rise to the advanced AMD phenotypes characterized by angiogenesis and/or cell death. Genes regulating these immunological activities, along with numerous other genes identified here, represent promising new targets for AMD-directed therapeutics and diagnostics.

    View details for DOI 10.1186/PREACCEPT-1418491035586234

    View details for Web of Science ID 000314566500002

    View details for PubMedID 22364233

  • A proteomic approach for the identification of novel lysine methyltransferase substrates EPIGENETICS & CHROMATIN Levy, D., Liu, C. L., Yang, Z., Newman, A. M., Alizadeh, A. A., Utz, P. J., Gozani, O. 2011; 4

    Signaling via protein lysine methylation has been proposed to play a central role in the regulation of many physiologic and pathologic programs. In contrast to other post-translational modifications such as phosphorylation, proteome-wide approaches to investigate lysine methylation networks do not exist.In the current study, we used the ProtoArray® platform, containing over 9,500 human proteins, and developed and optimized a system for proteome-wide identification of novel methylation events catalyzed by the protein lysine methyltransferase (PKMT) SETD6. This enzyme had previously been shown to methylate the transcription factor RelA, but it was not known whether SETD6 had other substrates. By using two independent detection approaches, we identified novel candidate substrates for SETD6, and verified that all targets tested in vitro and in cells were genuine substrates.We describe a novel proteome-wide methodology for the identification of new PKMT substrates. This technological advance may lead to a better understanding of the enzymatic activity and substrate specificity of the large number (more than 50) PKMTs present in the human proteome, most of which are uncharacterized.

    View details for DOI 10.1186/1756-8935-4-19

    View details for Web of Science ID 000296832600001

    View details for PubMedID 22024134

    View details for PubMedCentralID PMC3212905

  • Global Analysis of Proline-Rich Tandem Repeat Proteins Reveals Broad Phylogenetic Diversity in Plant Secretomes PLOS ONE Newman, A. M., Cooper, J. B. 2011; 6 (8)

    Cell walls, constructed by precisely choreographed changes in the plant secretome, play critical roles in plant cell physiology and development. Along with structural polysaccharides, secreted proline-rich Tandem Repeat Proteins (TRPs) are important for cell wall function, yet the evolutionary diversity of these structural TRPs remains virtually unexplored. Using a systems-level computational approach to analyze taxonomically diverse plant sequence data, we identified 31 distinct Pro-rich TRP classes targeted for secretion. This analysis expands upon the known phylogenetic diversity of extensins, the most widely studied class of wall structural proteins, and demonstrates that extensins evolved before plant vascularization. Our results also show that most Pro-rich TRP classes have unexpectedly restricted evolutionary distributions, revealing considerable differences in plant secretome signatures that define unexplored diversity.

    View details for DOI 10.1371/journal.pone.0023167

    View details for Web of Science ID 000293511900032

    View details for PubMedID 21829715

  • clusterMaker: a multi-algorithm clustering plugin for Cytoscape BMC BIOINFORMATICS Morris, J. H., Apeltsin, L., Newman, A. M. (co-first author) , Baumbach, J., Wittkop, T., Su, G., Bader, G. D., Ferrin, T. E. 2011; 12: 436

    View details for DOI 10.1186/1471-2105-12-436

  • AutoSOME: a clustering method for identifying gene expression modules without prior knowledge of cluster number BMC BIOINFORMATICS Newman, A. M., Cooper, J. B. 2010; 11

    Clustering the information content of large high-dimensional gene expression datasets has widespread application in "omics" biology. Unfortunately, the underlying structure of these natural datasets is often fuzzy, and the computational identification of data clusters generally requires knowledge about cluster number and geometry.We integrated strategies from machine learning, cartography, and graph theory into a new informatics method for automatically clustering self-organizing map ensembles of high-dimensional data. Our new method, called AutoSOME, readily identifies discrete and fuzzy data clusters without prior knowledge of cluster number or structure in diverse datasets including whole genome microarray data. Visualization of AutoSOME output using network diagrams and differential heat maps reveals unexpected variation among well-characterized cancer cell lines. Co-expression analysis of data from human embryonic and induced pluripotent stem cells using AutoSOME identifies >3400 up-regulated genes associated with pluripotency, and indicates that a recently identified protein-protein interaction network characterizing pluripotency was underestimated by a factor of four.By effectively extracting important information from high-dimensional microarray data without prior knowledge or the need for data filtration, AutoSOME can yield systems-level insights from whole genome microarray expression studies. Due to its generality, this new method should also have practical utility for a variety of data-intensive applications, including the results of deep sequencing experiments. AutoSOME is available for download at webcite.

    View details for DOI 10.1186/1471-2105-11-117

    View details for Web of Science ID 000276296100002

    View details for PubMedID 20202218

  • XSTREAM: A practical algorithm for identification and architecture modeling of tandem repeats in protein sequences BMC BIOINFORMATICS Newman, A. M., Cooper, J. B. 2007; 8

    Biological sequence repeats arranged in tandem patterns are widespread in DNA and proteins. While many software tools have been designed to detect DNA tandem repeats (TRs), useful algorithms for identifying protein TRs with varied levels of degeneracy are still needed.To address limitations of current repeat identification methods, and to provide an efficient and flexible algorithm for the detection and analysis of TRs in protein sequences, we designed and implemented a new computational method called XSTREAM. Running time tests confirm the practicality of XSTREAM for analyses of multi-genome datasets. Each of the key capabilities of XSTREAM (e.g., merging, nesting, long-period detection, and TR architecture modeling) are demonstrated using anecdotal examples, and the utility of XSTREAM for identifying TR proteins was validated using data from a recently published paper.We show that XSTREAM is a practical and valuable tool for TR detection in protein and nucleotide sequences at the multi-genome scale, and an effective tool for modeling TR domains with diverse architectures and varied levels of degeneracy. Because of these useful features, XSTREAM has significant potential for the discovery of naturally-evolved modular proteins with applications for engineering novel biostructural and biomimetic materials, and identifying new vaccine and diagnostic targets.

    View details for DOI 10.1186/1471-2105-8-382

    View details for Web of Science ID 000252936900001

    View details for PubMedID 17931424


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I Dreams In Colors

July 28, 2013

Hello… hello …hellooooooooooooo.

I’m still here. Yessuh.

Is anyone still here?

I promise I haven’t been in a coma and I’m not dead yet (neither is Dotty FYI). Where have I been? What have I been doing?

Well, obviously I’m not blogging. It’s been so long since I’ve blogged that I forgot my password a few weeks ago! (Yes I still log in to read some of your blogs occasionally). Anyway, I PANICKED! When does P not panic you may ask… Well, fuck, I didn’t NOT panic the day I logged in and couldn’t remember my password–had to go through the cumbersome ordeal of resetting my password and all.

Fortunately enough, I have a bank account now and I used the bank account password as my new WP password.

Fuck, where was I going?

Oh yeah, it’s 3am and the weekend. I’ve spent a lot of my time working lately, not just AT WORK, but even at home. Call me busy bee, or busy P or whatnot.

So, on my time off, I’ve been drawing again instead of blogging. These last couple of weeks I’ve been slacking off on the daily sketches. I was lying here, trying to get myself to bed, just puffing up my pillows, when I decided to doodle Little Luna from memory.

Maybe I’ll post some of the sketches I did last month and the one before and the one before that. I’ve just got too much to say and not enough time. Some not-so-nice things and some very, very nice things.

I’ve got a new “art” blog going, though I haven’t been updating on that one either. And I’m working on my website. Still in therapy, still swimming (even though I had to stop for a month).

I guess you could say I’m fairing well, mostly.

Night night.

Mouse love

Luna Sketch

“I dreams in color”
pen and ink © paz 2013

9 Responses to “I Dreams In Colors”

  1. Glad to hear from you, to know you’re still around, fighting the good fight. 🙂

    • PAZ said

      Glad to know you’re still around too. I’ve been meaning to email you I read you last post on Canvas and it made me sad. How are you feeling?

  2. Hello Mouse! Good to see you!!

  3. Hey P, nice to see your shining virtual face again! Hope this bodes well for more in the near future. I’m in Israel and just got bitten by something soft and squishy! Damn, I didn’t know such things existed here. Ugh.

    • PAZ said

      Where did you get bit? Where you in the ocean? I don’t think I’ll ever catch up as much as I’d like with my Canvas fam, but this will suffice.

      • I got bit right in my own bed (well, cot, really, but that’s another story). Since then I have discovered what it was that bit me. About 3/4 inch long, teardrop shape, whitish, scuttles on many dreadful patitas (or is it patitos, I forget?) and BITES! Damn! I squished the sonovabitch. I tell you what, one thing that gets my back up is uninvited critters in my living quarters. There are cucarachas the size of chihuahuas here. I’m not kidding. They are called “jookim” in Hebrew. That cracks me up, you know? I kills them.

        • PAZ said

          It’s “patitas” since you’re female, so yes, you got it correct. Oh GOT CUCARACHAS?! Do they fly all around?! Those are the WORST! I keep getting bit by mosquitos. It seems they all fly in the house straight for me because I end up with thirteen bites in one night and then ask my parents if they got bit. Nope.

          • That’s ’cause you’re sweet. In Hebrew they’re yetushim. We have cucarachas the size of volkswagons, call them jukim. Guess that’s cause they juke all around. They make a satisfying crunch when you whack the bastards with a dust pan. Then there’s these little shiny black flying things with stingers, I don’t know what they are but I stay far away from them!

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warcinfo:
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publisher: Common Crawl
description: Wide crawl of the web for September 2018
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Chalk paint kitchen cabinets pinterest

Related Post from Chalk paint kitchen cabinets pinterest

  • New Ideas Corner Nook Kitchen Table

    Update your corner nook kitchen table colors and details that complement accompanying cooking and according to their current needs. Redo your breakfast nook can be as simple as new furniture and paint

  • Stylish Travertine Backsplash Tiles Kitchen

    Travertine tile has a warm appearance suitable for many areas of house. From travertine in its natural state has a rustic and worn look, which can be used to enhance natural, rustic and informal desig

  • Standard Counter Height Kitchen Table

    Counter height kitchen table – High table is important, especially when you go to buy a new table. Add 6 inches to tables of art or work; That it measured up to 42 inches. Any size for different


text:
Paris Lettau
Andrew Browne, Spill

Andrew Browne, Spill

Tolarno Galleries, Level 4/104 Exhibition Street, 7 June – 7 July 2018

By Paris Lettau

In the final chapter of his 1931 book, An Account of French Painting, Clive Bell attempts to explain what he calls 19th century French painting's (i.e., modernism's) 'momentous lapse in taste'. Of course, he meant a lapse from the perspective of those trained in the 19th century Academy's tradition of taste, and all the standards of painting it sought to uphold. For Bell's generation, on the contrary, the late 19th century was seen as a new beginning for painting: whereas previous generations had followed tradition and imitated the masters of the Academy, Bell said, modernists sought an original genius, to break from institutional embeddedness, to present a pastiche of all that was on offer; a new synthesis of any style, genre and technique, symbolised by that eclectic tableau, The Luncheon on the Grass, by Édouard Manet, famously rejected by the jury of the 1863 Paris Salon.

But if the 19th century produced modern masters, Bell argued it also produced more bad art than any other century. For by imitating the masters of the Academy, an otherwise peripheral painter was at least capable of being acceptable. Only when the work of an original genius, having confused all the examples of the old masters, comes impure before the public is it recognised for what it is—something profoundly disquieting.

To call Andrew Browne's practice 'disquieting' would, of course, be to say nothing new. In fact, a scan of the literature on his work shows that this, in some register or another, is about the only consistent thing said about his gothic greyscale drawing, painting and photography: it is disquieting, filled with suspense, dream-like, humming with mystery, with a sense of desolation, an uneasy mood of chaos and unpredictability, an anthropomorphic and menacing romance.

Andrew Browne, ‘Spill’, installation shot 2018, courtesy the artist and Tolarno Galleries.

These could all be obvious claims of the works in Browne's current show, Spill. The first five framed charcoal on paper works, for instance, all display this sense of disquietude: a fawnish romanticism that's deliberately fantastical. Frozen, for example, shows a glacial flow of water arrested in charcoal, recalling in black and white the eerie and fantastical atmosphere of William Blake's 19th century illustrations to John Milton's Paradise Lost. At the same time, though, there's something altogether mundane about these works, as if their disquietude were just an afterthought or an after-effect, a result of Photoshop editing: in Drawing (above left), for example, it looks as if a black and white photograph has been digitally inverted to produce the effect of a photographic negative and a hand-drawn 'vibe'.

This is not to say the works are a gimmick, but that the uncanniness of the images seems somehow artificial, almost beside the point, in the same way that the subject matter of a post-impressionist painting—take for example the nude in Emmanuel Phillip Fox's Summer (circa 1912)—is a neutral foil for the artist's intended study of light, colour, texture and atmosphere; or if not a neutral foil, then a conflicted artistic intent in which a formal study fights a 'mysterious' effect, and vice-versa.

Andrew Browne, Frozen 2018, Charcoal on paper, 80.2 x 121 cm, courtesy the artist and Tolarno Galleries.

This doubling of half-intents reads almost as a confusion of style, as if Browne's work half accidently, half deliberately, acts against itself and undermines its own elements. It is in this rather roundabout sense that there is something disquieting about Browne's work; not disquieting in the sense of the uncanny, but in the sense meant by Bell: that it seems to escape the properties an academically trained taste is brought up to identify and admire, as if the works were judging taste itself. In a similar way, that is, that the jury of the Paris Salon was disquieted by, and so sought to ban from public view, Manet's Luncheon: a painting that combined elements of genres that were supposed to be hierarchically separated.

Andrew Browne, ‘Spill’, installation shot 2018, courtesy the artist and Tolarno Galleries.

A similar point can be made about Descent, a large oil on linen in which the compositional logic of 'downwardness' is heightened by scale—as well as by artificiality. This downwardness forms part of the show's premise, and is derived from its title Spill, an abstraction from the idea of falling water, which is itself an abstraction from photographs of waterfalls taken by the artist. The work is partly reminiscent of Gerhard Richter's abstracts and photo paintings, such as the blurred Uncle Rudi, 1965, or his well-known overpainted photographs. But Descent lacks that naturalised Richter-esque synthetic tension between photograph and paint seen in the overpainted photographs, for example—that unified pressure of material paint against the ideality of the photograph; an ideality that, in turn, both holds onto the paint and renders it irreal and weightless. Unlike Richter's photographic surface, in Descent the black primed linen does not synthesise with its overlayed paint into a resolved compositional tension. Each layer floats passively up against the other. Even fateful splatters of paint abolish their sense of chance, in that each one feels half intended, artificial, placed. The surface is slippery like a perimeter fence, unwelcome to the paint that descends its surface.

Andrew Browne, Descent 2018, Oil on linen, 240 x 175 cm, courtesy the artist and Tolarno Galleries.

One work in Spill is offered by Tolarno as if it aspired to the virtual heights of the prized genres of 19th century Academic painting. According to Tolarno's website, Threshold measures an immense 150 x 605 cm, thus presenting itself in the grand tradition of history painting, like Benjamin Duterrau's enormous and now lost A National Picture, 1840, currently being projected onto the walls of the National Gallery of Australia, or Gustav Courbet's gigantic A Burial At Ornans, 1849-50 (that great metaphor for the burial of the Academy's hierarchy of genres). The huge scale of Threshold thus far eclipses the dimensions of the wall it hangs on, which must be less than 500 cm. If the dimensions are an obvious typo (one reproduced across the internet), it is a particularly interesting one. Not only does it suggest an extraordinary scale of ambition on the part of the artist, it also suggests an immense, if virtual, breadth of genres and styles across work presented in Spill.

Andrew Browne, Threshold 2018, Oil on linen, 152 x 605 cm, courtesy the artist and Tolarno Galleries.

In fact, Threshold also recalls Sydney Long's symbolist art nouveau (yet another 'style') arcadias, such as Pan, 1898 (close to Threshold's actual, rather than mistyped dimensions), populated as it is with nymphs and satyrs. If Browne, like Long, alienates the landscape of the picturesque, Long's transferal of the melancholic onto the Australian landscape is missing in Browne. In fact, Threshold lacks all signs of place to truly be described as Australian gothic abstraction; its utopic, cartoonish netherworld, is populated instead by a shadowy, ambiguous urban Pan standing on a horizontally aligned turnip-like earth.

The flattest of all the works—and smallest—Silver Spill, breaks with the idealised downward direction (i.e., the idea of the 'spill' of water) of the show to present a far more even, uniform modernist space. The layering of swathed brush strokes on black primed linen recalls Francis Bacon's 1953 Study after Velázquez's Portrait of Pope Innocent X, except it lacks that sense of a frightful breakout into the abyssal nothing of the painterly ground. In Silver Spill, everything floats forward, once again, in a virtual rather than existential (or even ontological) space.

Andrew Browne, ‘Spill’, installation shot 2018, courtesy the artist and Tolarno Galleries.

The verticality of the picture support, hung like a portrait, follows that of most the works in the show, only it doesn't use the downward movement of falling water to derive its compositional logic. In Silver Spill, the light silver-ish foreground 'spill' falls up as much as down. The middle-ground swathed brush strokes make the same double move up and down, from a stroke beginning in the lower right corner and lifted upward, to another beginning in the upper right corner and pulled down. Downwardness is also thwarted by the central 'opening' of the foreground silver spill, which anchors and centres the picture, but which is also unbalanced by a second opening slightly above it—and each framing a few splatters of paint as if, like the impressionists, the artist sought to capture a fleeting moment of chance, to harden and abolish accident.

Browne half-deliberately hovers in this virtual space between impure mixture and original synthesis in a way that makes his work especially, if obliquly, interesting, like Manet's new proposition of what a successful tableau could be. This is what is so disquieting but also compelling about Browne's practice—even its paradox—that it ultimately appears, like Manet did in the 19th century, as an eclectic yet persuasive pastiche, in which each stylistic effect counter-poses against its other: melancholy symbolism with post-impressionist study of light, colour, texture and atmosphere; modernist space with romantic uncanniness; history painting with Baconian chaos and disintegration; Richter-esque synthesis of painting and photography, but without synthesis. A cogent practice of pastiche hidden beneath a thick veneer of gothic greyscale.

Paris Lettau is writer from Melbourne.

Top image: Andrew Browne, Silver spill 2018, Oil on linen, 61 x 41cm, courtesy the artist and Tolarno Galleries.


text:
I was syncing with Dropbox, and Mental Case 2.3 lost my data. How do I get it back?

Mental Case 2.3 introduced sandboxing, which is now a requirement for all apps in the Mac App Store. If you were using Dropbox syncing, with a symbolic link to the Library folder, the migration to sandboxing will not import your data properly.

Here is the fix:
1) Remove the symbolic link at /Library/Application Support/Mental Case
2) Drag that data back from Dropbox to the place where the symbolic link was
3) Go to the folder

4) Move the folder com.mentalfaculy.mentalcase.mac to your Desktop
5) Launch Mental Case 2.3. It should copy the Library folder you moved from Dropbox into the new Container, and it should work after that.

Note that to get to the /Library folder, which is hidden, you need to select your home folder in Finder, and then choose Go > Go to Folder, and enter 'Library'.


text:
 Watching Regional News for a Mexican Community as You Travel – Insurance For Mexico

Watching Regional News for a Mexican Community as You Travel – Insurance For Mexico

Mexican insurance,Mexican auto insurance,Mexico insurance
Insurance For Mexico El Hispanic News

Insurance For Mexico – Watching Regional News for a Mexican Community as You Travel

You might not read Spanish, but it is wise to pay attention to local resources when you are on an extended trip through Mexico. It’s easy to panic from a distance about issues such as hurricanes and volcanoes, but a combination of local and international resources can set your mind at ease or prompt decisive action. Your insurance for Mexico won’t keep you safe from severe weather events, but it could prove very helpful if you are stranded on the road because of a problem. Your Mexican insurance quote online provides you with access to tourist services, including roadside assistance and emergency towing.

How Common Are Serious Natural Events?

The 2015 hurricane season in the Pacific has seen a record number of events. A hurricane could occur at any point during the year, but they are typically restricted to the period from May 15 to November 30. Similarly, major earthquakes and volcano eruptions are not common. However, the possibility of these events makes it important to be alert as you travel. Occasionally, an unusual weather event can cause major road damage even if the weather wasn’t particularly severe. This occurred in recent history in Baja Norte as the Ensenada Toll Road was closed for a long period due to a landslide that damaged the road.

The Role of Your Insurance for Mexico

Your Mexican insurance quote is required by the government to ensure that your financial obligations are handled in case of a wreck. It is helpful to be aware of supplemental coverage that works with your insurance for Mexico if an accident occurs on a toll road. You are not singled out as a tourist. In fact, all motorists are expected to carry seguros to cover their potential obligations in accident situations. As a traveler, you may only be going into a border zone, but you still need to purchase a reputable Mexican insurance quote . When you get a Mexican insurance quote, your travel plans dictate the best choices in liability protection, timing, and add-on benefits.

Each insurance for Mexico policy by MexicanInsuranceStore includes Roadside Assistance throughout all of Mexico at no additional cost for your peace of mind!

Learn more about the best Mexican insurance quote and an insurance for Mexico policy here

Insurance For Mexico – Watching Regional for a Mexican Community as You Travel

{ 1 comment… read it below or add one }

Roni Latham December 8, 2016 at 4:10 pm

The threat of a fatal accident is 3x higher in the evening. This is where you need most of your Mexico insurance.

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Lovely Pink Dolphin sighted in Louisiana

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The world has seen dolphins but not much of this rare pink-colored specie which was spotted playing in Louisiana ship channel.

Yes! Pink dolphins do exist.

Bridget Boudreaux says she spotted “Pinky” while on a boat cruise with her husband last weekend.

Pink Dolphin

The couple were on a jolly ride in the Calcasieu Ship Channel near Hackberry on Saturday afternoon when by surprise, they caught a pink dolphin strutting with reckless abandon.

“I nearly jumped out of the boat,” said Boudreaux.

I was like wow that’s not a regular dolphin, that’s a pink dolphin.”

She said they got a bit close and noticed there were two pink dolphins around the spot, but her camera clicks could get only one. How disappointing!

Boudreaux badly wanted us to see a picture of the awesome pair.

The pink dolphins were originally seen about 20 feet from their boat. They were reportedly playing among others of their kind.

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How to Blaze Past the Bullshit

I could write endless blog posts about the who and the what that pisses me off. Honestly, I don't have enough time and even if I did, I wouldn't want to read or listen to it. That's why I'm not on Social Media and if you are, you need to read this article it's because you have not yet learned to Blaze Past the Bullshit.

That being said, I am going to share how I Blaze Past the Bullshit. Here goes:

  1. I understand the finality of time - In my 14 Things a Real Artist does podcast, I talk about the finality of time. We only have so much of it and out of that time, only a fraction of it will provide the optimal level of critical thinking and focus necessary to produce something worthwhile. That being said what you find important will blaze past all the bullshit of what is not important. Things that initially bothered me (even things I have written about on this website) may no longer bother me. Pursuits I once had I may no longer want to pursue out of priority for something else. Whatever the case, I understand that my time is valuable and I don't want to waste it on bullshit I can blaze past.
  2. I understand the law of numbers - I don't hold any person, situation or anything for that matter in enough esteem to be married to it. I understand that there will always be a new opportunity out there when you spend everyday hustling. This is why although I may write about females ghosting or small type situations, I don't dwell on them, especially after I have already addressed them. I know that I have bigger fish to fry and that I'm never going to get involved in a deal or situation that could be beneficial if I'm worrying about all the ones that don't work out.
  3. I understand the power of being real - When you tell the truth, you don't have to keep up with your lies. But people like to lie, deceive and throw filters on who they are so they are not rejected by the tribe. I know that being fake is a fundamental human condition which is why being real can be such a valuable character trait.
  4. I take risks and I'm not afraid to lose or fail - Like I talk about in fanmail podcast 1, I know how to fail, that's why I succeed. I even mention that I don't do it with style and grace. I am a master of the blowout fail. The embarrassing fail. The make you want to quit type of fail. BUT...there is always a BUT these fails have built a callous around who I am. It has hardened my shell and ultimately allowed me to stand in a league of my own because I am not afraid to fail.
  5. I am direct with people - Similar to understanding the power of being real. As I high value male, if I ghost you, it's because I don't have time. But if I do, I will tell you exactly why it didn't work so that we can both move on and become better people in the process. I don't like beating around the bush because it doesn't help anyone.
  6. I don't tolerate negativity - I discuss a lot of this in the How a High Value Male Operates podcast. The fact that we don't tolerate negativity and it's true. I don't entertain negativity or have anyone on my team that acts in a negative fashion. Complainers, excuse makers and anything else. They all have to go.
  7. I let go and move on - I don't hold on to girls that don't want me around, I don't hold on to people that don't treat me how I should be treated, I don't let past versions of myself keep me from evolving. While nostalgia can be an important form of reflection for your progress, I don't spend all my moments with high school friends so to speak. I understand that in order to go I need to let go of past situations to get to new ones.
  8. I don't forget and I write things down so I don't. - I don't forget people and situations that have been both positive and negative in my life, so that I remember to not revisit certain experiences which created a setback for me. I also don't forget people that my have helped me or expressed an interest in working together but for some reason we just weren't able to sync up at that particular time
  9. I know that you can't take on everything - The Manicotti brand is a perfect example of taking everything on. I'm doing blogs, music, podcasts. All of this stuff is part of my process to be the greatest version of myself that I can. My website has been critical in holding me accountable to that. Can you imagine if I took on more? It's possible, but before I do I will need to automate more because I can't do it all.
  10. I try to delegate the menial - There is lots of stuff you shouldn't be doing. The busier you get and the more money you make the more you will realize what that is. When you do, delegate it. I find a way to outsource those tasks so that I can move on to more important things. I try to find people that are better than me at a given task as well. I utilize their mastery and unique skills to get done what I need done and I don't try to learn it myself. I could have spent weeks or months replacing the siding on my house, but that's not my expertise and it was better for me to spend the money paying an expert so I could write posts like this.
  11. I don't get sucked into other people's drama - There are a significant amount of people that want to dump their drama on you once you make them comfortable. The second I sense that happening, I either establish boundaries or cut them lose. This speaks to the point on negativity. It sounds harsh, but it's really not. People have done this same thing with me in the past. They trained me to change my mind frame and what type of behavior they would expect out of me in order to deal with them. Once I learned this I complied and it wasn't so bad because it actually made me a better person.
  12. I stay true to the process and trim the fat as I go - When you create, you know what will eventually outlive its usefulness and where you're going to need to thin the herd or trim the fat. But you have to go through the process before you do that. The more you do it the more things will
  13. I understand that perfection is a death wish - If you look at my website and content there are a shitload of spelling, grammatical errors and overall ways things could be improved. But it's still good enough to get my point across. If you can't understand that I'm not perfect then you're missing the point of my whole pursuit. This is all part of the process. The thing about perfection is that it is a huge time waster. Not only is it a time waster but it's going to prevent you from getting to every issue that you need to address in your lifetime. I have made this mistake far too much in my life. From songs to business endeavors, whatever. What I learned over time is that as long as it is good enough to get it done and get the point across then it's good enough. In other words I remove perfection from the equation because I know it's a death wish.
  14. Whatever happens, I just keep moving - There are all kinds of famous sayings on this. No matter how fast or how slow, just keep moving!! Sharks that don't keep swimming die!! You know how all that goes, but it's true! Today for example I was hung over and uninspired to write this or any other podcast material...but I had to keep moving. So I went and rode 13 miles on my fixed gear bike and here I am writing because I kept moving.
  15. I forgive - I'm sure there are lots of people that feel they are owed an apology for something I did to them. On the flipside there are lots of people that owe me an apology as well. It's human nature to fuck up and then ball up and pretend like nothing happened. But if I do something that has hurt someone, I eventually crawl out of my hole and apologize for the errors in my ways, if I did in fact hurt them. If I didn't and they believe I did, I will clarify why I felt that way but some people hold grudges because they are lesser evolved than you and there is really nothing you can do to change their mind. You just have to agree to disagree and respect each other's space. I have lots of stories about people that have committed unforgiveable offenses against me, that I forgave anyways because I could tell they were genuinely apologetic about their actions. I guess the bottom line is that I try to forgive people and just move on.
  16. I don't use social media for anything other than business - Y'all know this from my How Social Media is Destroying Your Life podcast. Social media really has no purpose other than to sell shit and distract people. So if you're not selling something to someone, you're being distracted by someone trying to sell something to you. It is a form of media you need to blaze past to focus on what you are trying to do whatever that may be. If the average human spent the 1 hour a day they use posting banter on social media and read books, we'd have a much more productive and educated society. I won't even say it's sad. It's just true and you know it. That being said, get off all social media and focus on what you're supposed to be doing.


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Priority Club: 1,000 Points For Your Thoughts

Last year one of my most popular posts was one where I was giving away codes to watch a 90 second video to earn 1,000 Priority Club points.

I’m pretty sure that everyone loves free points so I figured I might as well share another offer that I saw a while back on frequent flyer site Milepoint.

Milepoint member Miles and Smiles created a post about filling out a quick survey to earn 1,000 PC points.
According to the terms, only Priority Club Rewards members that received the offer by e-mail are eligible to participate. Bonus points get deposited 4-6 weeks after completion of the survey.

I didn’t receive an e-mail for this offer but decided to take a chance and fill out the survey.
Now I’m just hoping that the 1,000 bonus points post to my account.

I figured that I had nothing to lose and neither do you.

If you have a Priority Club account, make sure to fill out the survey HERE and take a chance at being rewarded with an easy 1,000 points

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Win A Trip For 2 to the New York City Wine & Food Festival

Screen Shot 2013-09-16 at 6.11.51 PM

I just saw a tweet from one of my favorite television food personalities about a contest with a great prize.

Andrew Zimmern tweeted about a contest where you could win a trip for two to the New York City Food and Wine Festival which takes place  from October 17-20.

The prize includes:

  • Air travel and accommodations for 2
  • $500 spending money from Daily Worth
  • $500 from Food52’s new kitchen & home shop- Provisions
  • Two tickets to six of the festival’s best events including:
  1. Rockin’ Dumplings and Rolling Sushi by EATER hosted by Masaharu Morimoto
  2. Blue Moon Burger Bash by Pat LaFrieda Meats hosted by Rachel Ray
  3. Olmeca Altos Tequila presents Tacos and Tequila hosted by Bobby Flay

Some Rules:

  • By entering the contest you agree to receive e-mails from Eater, New York City Win & Food Festival, Daily Worth and Food52. (You can unsubscribe at any time.)
  • Must be 21+ to enter
  • Contest ends September 25

Enter for a shot to win a trip to the New York City Wine and Food Festival HERE

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art of resistance, Israel, Israeli - Palestinian conflict, Palestine, Syria

Must-See Palestinian Movies.

I don’t know about you, but outside my window there’s rain. It’s a chilly saturday. And I am not working today. It’s a perfect time for books, movies, some tea.

So, if you’re now or sometime in the same situation, here are some great palestinian movies to put on your watchlist (palestinian cinema/cinema dealing with israeli-palestinian issues is among my favorites).

AJAMI (2009)

AJAMI1Ajami still

The movie revolves around Ajami, religiously mixed community of Muslims and Christians in Tel Aviv. There are five stories about the everyday life in Ajami, mixed together. The mode of storytelling  is amazing and powerful – a character will appear two chapters later only for his intentions to be revealed then. It’s a modern cinematic piece. There are drugs involved, violence, dark little streets, fury, clashes… If you didn’t know it’s set in Tel Aviv, it could have easily been somewhere in the Latin America, or some ghetto in the outskirts of Paris. Also, the movie was made by a palestinian and and an israeli director, so it’s a masterful cooperation on more than one level. Watch it!

It’s a story of a Palestinian widow who must defend her lemontree field when a new Israeli Defense Minister moves next to her and threatens to have her lemon grove torn down. It might seem banal, but a tree is much more than just a tree. It represents land, hard work, survival –  it represents life. The movie touches on many issues – israeli occuppation through everyday life, but also the lack of will among palestinian officials – in this case – Salma, the widow,  gets no help from the local Palestinian politicians, who don’t like rocking the boat. It’s a circle of desperation. Movie was done by an israeli director, Eran Riklis, who did several other great films, including The Syrian Bride.

tumblr_mxa0jvDPnn1ql94czo2_1280 tumblr_mxa0jvDPnn1ql94czo3_r1_1280 tumblr_mxa0jvDPnn1ql94czo4_1280 tumblr_mxa0jvDPnn1ql94czo5_1280 tumblr_mxa0jvDPnn1ql94czo6_1280 tumblr_mxa0jvDPnn1ql94czo7_1280 tumblr_mxa0jvDPnn1ql94czo8_1280 tumblr_mxa0jvDPnn1ql94czo9_1280Lemon tree stills

It’s a movie about two childhood friends. Who are recruited for a suicide bombing in Tel Aviv. Yeah, it’s quite hard. Said and Khaled, who have volunteered to be suicide bombers, receive word it will be tomorrow – the cell’s first operation in two years.

tumblr_lo37ueLzJ91qixuo1o1_500Paradise now still

Something goes wrong at the crossing, they’re separated, and the action is postponed. Just long enough for renewed questioning of what they’re about to do. Oh my, there are so many thoughts in their heads.

tumblr_leida7lWpG1qdxzvxo1_500Paradise now still

Where’s the solution? Is this the solution? Could there be a solution at all? The film explains a difficult situation without taking a stand or forcing you to do so. Brilliant and a must see (just like other movies Hany Abu- Assad did, great director).

It’s a story a working-class woman and her experiences traveling back home. Soraya was born and raised in Brooklyn, NY, and one day discovers that her grandfather’s savings were frozen in a bank account in Jaffa when he was exiled in 1948.  Determined, she fulfills her life-long dream of “returning” to Palestine. Finally in Palestine, she meets Emad, a young Palestinian whose ambition, contrary to hers, is to leave forever. He’s tired of the constraints that dictate the lives under oppression. It is a deeply layered film, a film that is quasi-militant and seeped in passion, but devoid of hatred of any kind. Annemarie Jacir is the director. I generally enjoy all of her artistic work, she’s one of the great palestinian talents.

salt of this seaSalt of this sea, still

Now, this is another, more recent movie done by Jacir. The year is 1967. and  in Jordan, the world is changing. Tens of thousands of refugees pour across the border from Palestine. Having been separated from his father in the chaos of war, Tarek, 11, and his mother Ghaydaa, are amongst this latest wave of refugees. Placed in “temporary” refugee camps made up of tents and prefab houses until they would be able to return, they wait, like the generation before them who arrived in 1948. Tarek searches a way out, and discovers a new hope emerging with the times. Eventually his free spirit and curious nature lead him to a group of people on a journey that will change their lives. Love, humor, desire to be free – the movie has it all. Inspiring.

When_I_Saw_09When I saw you still

OMAR (2013)

We’ve heard a lot about Omar this year, and fairly so. The director is Hany Abu-Assad (same as with The Paradise Now). He did it again! I was amazed when I saw it. It’s a movie about Omar, a young Palestinian freedom fighter who agrees to work as an informant after he’s tricked into an admission of guilt by association in the wake of an Israeli soldier’s killing. It’s a love story. And it’s so complicated. It’s a palestinian story indeed. Just when you think you know what is going to happen it all changes. The story is handled in a harsh, uncompromising manner, and, to skip the long praise –  it’s a must see, so see it!

0Omar, still

Enjoy the movies!

P.S. My all time favorite, when it comes to dealing with the palestinian issues, Waltz with Bashir, is not featured here, because there will be special posts dedicated to it.

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The Millennial Mom Times

Frequently Asked Questions and Answers– Instagram Edition

I recently posted on my Instagram story a photo, asking my viewers to direct message me questions they had regarding motherhood I got some questions that I had never been asked before, and the other [...] more

How We Discipline Our Toddler

For all intents and purposes of my blog, I think it is important I finally address how I manage to keep my toddler "under control" and well behaved He is two years and two months now so I wanted to [...] more

Dear Mastitis: Stay Gone

When you’re pregnant for the first time, and you tell your lactation consultant and nurse you want to breastfeed your newborn, no one tells you about the “M” word at the hospital Everyone is [...] more

Jade’s Birth Story

I didn't know what to expect when giving birth to my second child I often thought about my first pregnancy and how smooth the delivery went with Drew I'd read and heard so many stories-- good [...] more

Our Daughter’s Name Embroidery Hoop DIY Reveal

The day I found out the gender of my second child was the same day my fiancé announced to the family the name of our daughterBy now, everyone on my social media accounts know her name is going [...] more

Outdoor Gender Reveal: Paint Balloons and Darts

Our family and friends have wanted to know for some time now whether Drew and I were expecting a little Boy or Girl! If you read View A Miracle, then you already know Drew was the only one who knew [...] more

View A Miracle

At 17 weeks and 6 days, Drew and I went to our obgyn specialist in hopes of finding out the gender of our second child We agreed he would be the first to know the gender and the day of our reveal, I [...] more

DIY Mini Valentine’s Day Photo Shoot

Happy Valentine's Day from my family to you! As a new momma, I look forward to this holiday more so than any other I get to celebrate this day by showing my appreciation for my son in very creative [...] more


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Buy custom Abelard and Heloise

Essay's paper info
Topic: History
Number of pages / Number of words: 3 / 616
Essay's paper body

Neither Abelard nor Heloise seems able to think that Abelard treated his enemies badly. To Abelard, their entry into monasticism was, "Our conversion from the world to God," that is, it was done for the love of God.

Gender relations was a focus of Abelard's life, Heloise was not so concerned with that...

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Abelard desired that Heloise be submissive to him, just as any woman at that time should have been. In the time, men were thought to have owned women. Heloise was passed from her guardian, her uncle, to Abelard's control. Heloise, although, was not as willing to be submissive and humble as Abelard had desired, she was a progressive woman...

Essay fragment

General points of the essay

Cluniac Monasticism The Strengths and Weaknesses of Cluniac Monasticism Love Your Enemies Do You Agree That Brands That Chase Fads, Or Try To Create Them Will Fare Badly? Love, Sex, and the Gods in World Literature love calls us to the things of the world “Do You Agree That The Desire For Raw Materials Was The Most Important Reason For Japan’S Entry Into World War Ii?” Explain Your Answer Abelard and Heloise Love, Sex and Gender in the World Religions Monasticism And Intellectual L the best of enemies Heloise and Abelard 1st Letter Desert Fathers, Early Monasticism love your enemies Monasticism And The Code Of Chivalry

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Pork tenderloin with asparagus and warm citrus sauce

[Susan Hanna]

This recipe from the May 2014 issue of Canadian Living would be great for a casual spring dinner party. Pork tenderloin and asparagus are roasted, served over rice, topped with a warm sauce of ginger, garlic, chicken broth, orange juice and mustard, and garnished with almonds and parsley. It’s healthy, tasty and looks nice too. One note: if your asparagus is thin, it may be done before the pork is cooked. Either remove it and keep it warm, or add it to the pork after the meat is partially cooked.

pork asparagus

pork tenderloin with asparagus

 Avoiding Additives and Preservatives

 Check the label on the almonds to make sure no preservatives have been added. Use fresh lemon juice and orange juice and an all-natural chicken stock, such as Imagine brand. Many Dijon mustards contain sulfites; I use President’s Choice Old-Fashioned Dijon, which is additive free. For more recipes using all-natural ingredients, visit www.eyeforarecipe.ca.

  • ¼ cup (60 ml) natural almonds, chopped
  • 2 tbsp (30 ml) chopped fresh parsley
  • 2 tsp (10 ml) lemon juice
  • 400 g pork tenderloin, trimmed if necessary
  • ¼ tsp (1 ml) each salt and pepper
  • 1 tbsp (15 ml) olive oil
  • 2 bunches (each 450 g) asparagus, trimmed
  • 1 tbsp (15 ml) grated fresh ginger
  • 2 cloves garlic, minced
  • ¾ cup (175 ml) sodium-reduced chicken broth
  • ½ cup (125 ml) orange juice
  • 1 tsp (5 ml) Dijon mustard
  • ½ tsp (2 ml) grated orange zest
  • ¾ cup (175 ml) basmati rice

  1. Stir together almonds, parsley and lemon juice; set aside.
  2. Sprinkle pork with half each of the salt and pepper.
  3. In nonstick skillet, heat half of the oil over medium-high heat; brown pork all over, about 6 minutes.
  4. Transfer pork and asparagus to foil-lined rimmed baking sheet. Sprinkle asparagus with remaining salt and pepper.
  5. Bake in 400 F (200 C) oven until just a hint of pink remains inside pork or instant-read thermometer inserted into thickest part reads 160 F (71 C), about 15 minutes.
  6. Transfer pork to cutting board; tent with foil. Let rest for 5 minutes before slicing. Add remaining oil to skillet; heat over medium heat. Cook ginger and garlic, stirring, for 2 minutes. Stir in broth and orange juice; bring to boil. Stirring often, reduce sauce to ½ cup, 5 to 7 minutes.
  7. Stir in mustard and orange zest. Cook rice according to package directions; serve with pork, asparagus and sauce. Sprinkle with almond mixture. Serves 4.

From the May 2014 issue of Canadian Living


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Review: Charter’s The Portable Beat Reader

The Portable Beat Reader

Ann Charters

A collection of excerpts from many of the most important beat works and writers including Kerouac, Burroughs, Cassidy, and Ginsberg. I read this as a teenager eager to learn about the world outside the small Connecticut town in which I was raised, and boy did it deliver.

Back then, there was no Wikipedia, Kerouac and crew were just cultural references in the magazines I read, citations in the songs I liked. I’d puttered around a bit in some of their works (I think I’d taken a crack at On the Road) but I knew almost nothing about their work, and even less about their lives, before I read this.

For a kid like me, finding this in the store at the mall was, frankly life changing. I write this now at a remove of more than twenty years, so the specifics of what I thought of the book have vanished. I do remember pouring over the biographical material, and I remember adding scores of books to my “to be read” list.

The internet’s great and all, but there’s something to be said for having to figure it all out on your own.

At 17, this stuff like a drug, sending me off imagining a world much bigger, more dangerous, and more exciting than the life I was then living. Of course, now my view of these writers is far more critical. I see the rampant sexism now, the often clumsy writing, the chauvinism in all its forms, but then it was exciting, and new… at least to me.

Recommended for the enthusiast (and the teenager, circa 1992)

This was the cover on the edition I had.

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From Minecraft Wiki
(Redirected from Minecraft.jar)
Jump to: navigation, search
Java Edition.png

client.jar is the main code source for Minecraft. It is located in the %appdata%/.minecraft/versions/<version> directory on Windows, or the ../minecraft/versions/<version> directory in macOS. When using the latest version of the Minecraft launcher, it is named <gameversion>.jar

It can be opened using a file archiving program or a Java decompiler.

Jar versions[edit]

The client.jar file is found in its version folder. It is accompanied by a .json file that lists the version's attributes. Normal versions are available to choose from via the launcher, and snapshots are also available. It's even possible to download alpha versions, and create your own version file to install mods on. Note that creating a new version is the only way to install mods; the launcher keeps normal versions synced.

Files in client.jar[edit]

In client.jar many files can be found, they can be categorized like this:

  • Texture files: Textures used by Minecraft, all in .png format.
  • Class files: Compiled Java code that Minecraft uses for program logic. These files (classes) are named like "acq" or "qn" because they have been run through an obfuscation tool to hide the names of methods, variables and classes. However, they can still be disassembled to understand their functionality.
  • Language files: Language files are used to change the messages in the game (achievements, tooltips, menu). It does not affect in-game chat.
  • Text files: Files that contain easy to edit text, such as splashes and texture pack descriptions, another text file is achievements/map.txt that contain code for the creation of the achievements menu background.
  • The META-INF directory: Contains information about the game files.

File hierarchy[edit]

This section needs updating. Some of the information no longer applies to the latest version.

This is the current file list as of 1.8.7. Note that java class files have been omitted for brevity.

Promotional Content


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Racing Trousers Stripes - Miss Castelinhos
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Racing Trousers Stripes

Children engage in make believe for a number of reasons. It provides a safe setting to express fears and desires. When children participate in role play, they are integrating and strengthening previously acquired knowledge. Children who have better pretense and fantasy abilities also show better social competence, cognitive capabilities, and an ability to take the perspective of others.

Inspired by role play, READY TO PLAY allows every child to be the Hero in their own story.

Made from high quality organic cotton with OEKO-TEX® Standard 100 certification and Confidence in Textiles label. Manual screen printed with 100% solvent free eco ink.

The leg cuff allows the pants to “grow” with your child. It can be used rolled up and as the child grows, it can be extended to the correct length.

This garment was handmade with dedication to quality and craftsmanship. Imperfections in the overall appearance are a natural characteristic.

Subscribe to the mailing list and receive a 10% discount to my Etsy Shop.


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Posts Tagged ‘China painted teacup’

Oh, my!  April has almost passed and I’m just getting this posted…and I’m actually procrastinating, even now with what I should be doing.  ;^)  I’m in the final week of my next-to-last semester in professional writing!  Soooo, I’m supposed to be writing!  ;^-  (I am!)  Actually, I tried something new and set up the photos with this post as a draft, then came back and wrote the content later, then scheduled a date to post it — and that didn’t happen.  Hmmm — first time for me and this tiny corner of technology, but I’ll figure it out next time!

April’s teacup is from one of my favorite sets.  My Great-Aunt Lois, my paternal grandmother’s youngest sister, hand-painted this porcelain cup, years ago for me at the request of my mother.  Beautiful dogwood flowers are the theme.  I love to see the dogwoods in the spring.  I usually keep an eye on one spot in the park where I know a dogwood lives.  This year I missed it.  (I’m missing a lot, but that’s okay, because what I’m doing sort of makes up for it.)  ;^)

Aunt Lois and my Uncle Bob lived in New Mexico where I was born and many of my relatives still live.  Now, Aunt Lois and Uncle Bob live less than an hour from me in Oklahoma.  I love to stop in for a visit.  There are always smiles and welcoming hugs.

Each cup in this set is painted just a little differently in pinks, violets, and greens.

Aunt Lois painted a tiny  scene underneath each cup.  Each one of these is different, too.  What a treasure.

I photographed it when my wisteria was blooming, brief, but spectacular.  ;^)

The tea is one of my favorites ~~ a naturally sweet and fruity herbal by Tazo called Passion.  It has hibiscus in it.  It makes a nice hot tea for something different and is really good iced, as well, since we’ve already gone into the 90s in Oklahoma this spring!!!

I hope that you can take a moment to pour a cup of tea, relax for a moment, and take in the beauty of the day.  Thank you for stopping by!

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