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As a school for journalists, The Poynter Institute has long been associated with standard setting and "benchmarking excellence." In partnership with the National Press Photographer's Association 2003 Best of Photojournalism & Photojournalism on the Web Awards judging committee, Poynter is proud to present this year's award winners.

Over five days, judges evaluated nearly 27,000 digital images and 75 websites. Entries were submitted in 37 categories, resulting in 97 awards. The judges did not present awards in the Sports Picture Story and Sports Photographer of the Year categories.

Judges applied the journalistic measures of relevance, impact, integrity, photographic content and creativity in the still categories. New criteria of presentation quality, navigation, mastery of the technology and tools were used for online medium. We offer sincere thanks to all of the volunteers, and to Poynter's web and Information Technology teams, who did such an extraordinary job facilitating the NPPA Best of Photojournalism judging. --Kenny Irby

Click here to view a complete list of winners

Best of Photojournalism 2003: Stills
Cliff Edom's ‘New America Award’ - Jamie Francis, St. Petersburg Times
Newspaper Photographer of the Year  - Rick Loomis - Los Angeles Times
* Magazine Photographer of the Year - Ami Vitale Getty Images
Sports Photographer of the Year  - No Winner
International News
- Carolyn Cole - Los Angeles Times
International News Picture Story - Carolyn Cole - Los Angeles Times
General News  - David L Ryan - The Boston Globe
Domestic News - Brian Baer - The Sacramento Bee
Domestic News Picture Story  - Barry Gutierrez - Rocky Mountain News
Feature  - Larry Downing - REUTERS
Feature Picture Story  - Casper Dalhoff - freelance
Portrait and Personality  - Damon Winter - The Dallas Morning News
Pictorial - Jamie Francis - St. Petersburg Times
Computer Image Illustration - Carol Cleere - The Tampa Tribune
Conceptual Illustration - John Burgess - The Press Democrat
Nature & Environment - Tony Overman - The Olympian
Nature and Environment Picture Story - Pedro Ugarte - Agence France-Presse
Individual Sports Action - Steve Griffin - The Salt Lake Tribune
Team Sports Action - Benny Sieu - Milwaukee Journal Sentinel
Sports Feature - Cyrus McCrimmon - The Denver Post
Sports Picture Story - No Winner
Magazine Feature - Steve Liss - TIME Magazine
* Magazine News - Nir Elias - Reuters
* Magazine News Picture Story - Kristen ASHBURN - Contact Press Images
Magazine Feature Picture Story - Kurt Vinion - Spectrum Pictures
* denotes graphic material

BOP Withholds Sports Awards for the Second Year

Best of Photojournalism 2003: Picture Editing
List of WinnersList of Winners

The Best of Photojournalism on the Web 2003
See winning entries and read judges' comments.

Anne Conneen is a Visual Journalism Adjunct Faculty at the Poynter Institute. In her role as adjunct faculty for visual journalism, she is committed to teaching journalists the finer points of color, typography, and design in the news media. Provides practical lessons that teach participants the importance of understanding and interpreting content in visual storytelling. Emphasizes conceptual, creative thinking in a collaborative environment. Writes about design, information graphics, photojournalism, web design, and interactive media for Poynter Online in a column called The Design Desk. As Poynter's design editor, she conceptualizes, designs, and produces projects for Poynter Online and Poynter’s printed publications. A skilled visual journalist, Anne joined Poynter in 1997. Her previous five years were spent honing her craft at The Tennessean in Nashville where she was the design and graphics editor, design coordinator, and a page designer. Received the Award of Excellence from the Communication Arts Interactive Design competition in 2002. Designed Poynter Online, which launched in November of 2002. Anne holds a B.F.A., Ringling School of Art & Design, Sarasota, Fla.

Andrew DeVigal recently accepted a tenure-track faculty position in the School of Journalism at San Francisco State University.  He has been a visiting professional and was a Poynter Fellow in 2000, teaching and directing seminars in the area of New Media and Visual Journalism, and is now a frequent visiting faculty. He helped lead the Stanford-Poynter Project, research studying how users read online news using an Eye Tracking System.  Formerly he was an interface designer for Knight-Ridder New Media in San Jose, designing many of the early verticals offered by Real Cities, a producer for chicagotribune.com, shaping the look and format of the original Internet version. In his other life, DeVigal was an informational graphic artist for the Chicago Tribune and the Contra Costa Times. DeVigal has also redesigned several online publications including the Albany (N.Y.) Times Union website, timesunion.com. His work can also be appreciated from the design of Handelsblatt Interaktiv, Germany's financial daily, which was redesigned by Mario Garcia Media Group.

Alan Dorow began his career over 20 years ago by working as a photographer and designer at newspapers in Florida and Arizona. A graduate of Indiana University, he worked in New York for 10 years as a freelance photographer and photo editor at the New York Times Magazine. While spending a year working as a visiting professor at Rochester Institute of Technology in 1992, he developed a keen interest in new media technologies, and went on to form Tango Interactive, a multimedia development company, in 1995. In 1996, he started Story Line, an online publication featuring photography and video in new media format. Story Line became Journal E a year later, and went on to win numerous awards and recognitions, including two first place awards in the New Media category of Pictures of the Year and a recognition for Web Site of the Week by Communication Arts. Journal E recently became Musarium, and continues to publish high-quality online stories. He currently works as the publisher of Musarium and as the principal of Tango Interactive in Silver Spring, Md., where he lives with his wife Lauren and six-year-old daughter Sabrina.

Beth A. Keiser is a 1988 graduate of the University of Miami with a B.S. in psychology and a minor in marketing. While at the University, Beth began working for the school newspaper, the "Hurricane," as a photographer and with much encouragement and support of the school's photo department was able to do an internship in photography at the Miami Herald in 1988. After graduation, she freelanced for the Miami Herald and the Associated Press for two years before becoming full-time staff with the Herald in the Broward County bureau. After four years with the Herald, she left the paper and went to work for the Associated Press in Chicago, Ill., and then in New York City. At the Associated Press she covered many events, including the Olympics in Sydney, Australia and Nagano, Japan as well as witnessing history at the Oklahoma City Bombing, the funeral of Princess Diana, presidential campaigns and the coverage of the World Trade Center attacks for more than a year. In September of 2002, Beth resigned from the AP and is currently a photographer in New York City.

Jean-François Leroy is a journalist who is passionate about photography. He collaborated at Photo-Reporter, Le Photographe, Photo-Revue and Photo Magazine. At the same time, he reports for the agency Sipa-Press. In 1988, he becomes Dominique Isserman’s agent. In 1989, with Yann Arthus-Bertrand, he created “ 3 days in France,” an operation that painted the portrait of France in 1989, 150 years after the invention of photography. Since September 1989, he has run Visa pour l’image, an international photojournalism festival. In 1997, he became associated with Hachette-Filipacchi through the société Image-Evidence, which he is one of the managers of.

Michael Sargent is currently Vice President for News Services / Getty Images and joined Getty Images in May 2001. He now runs their news operation, after a 17-year stint at AFP (Agence France-Presse). Mike has 27 years of experience in the news industry, 11 of them in management. His news career began as a photojournalist, working on the staffs of five different major daily newspapers in North Carolina and Florida. In 1984, he joined AFP to work in Washington, D.C., at the start of their international photo service.  Shortly thereafter, he was assigned to cover the Reagan White House, and in 1988, he was recruited at the close of the Reagan administration to work as a White House photographer for George Bush Senior. Within a year, he returned to AFP in the role of Chief Photographer for the Americas and later served as the region’s Deputy Photo Director. In 1999 he became the Photo Director for AFP in the Asia-Pacific in Hong Kong, until being recruited by Getty in 2001.

Robert Seale has been a staff photographer at The Sporting News since 1996. In addition to covering major sporting events throughout the  U.S., he spends about half of his time shooting location portraits for the magazine. During his tenure at TSN, his photographs have appeared on more than 100 covers of the 117-year-old magazine. Prior to his position with The Sporting News, Robert was a staff photographer at The Houston Post and The Augusta Chronicle.  He is a graduate of Stephen F. Austin State University in Nacogdoches, Texas. He lives in Houston, Texas with his wife, Karen Warren, who is a photographer at The Houston Chronicle.

Horacio Villalobos is Director of Photography of Diario Popular in Buenos Aires, Argentina. He began his photojournalism career in 1965 in La Plata Buenos Aires for the newspaper El Dia. Villalobos did his post-graduate studies at the University of Missouri in 1974-75, as the recipient of the first photojournalism scholarship given by the Inter American Press Association (IAPA). He received several international photojournalism awards, among them from the IAPA in 1973. Beginning in 1972 - and for more than 25 years - Villalobos has done photographic work for Time, Newsweek and Business Week magazines, among others; United Press International and The Associated Press. He has covered everything from wars and violent revolutions to World Cup football and the Olympics in the Americas, Europe, Asia and Oceana. He began his career at Diario Popular, a Buenos Aires daily with national circulation, in 1976 and has been the newspapers’ director of photography since 1982. He has been a lecturer for the IAPA since 1980, and a teacher of seminars given by the Association of Journalistic Entities of Argentina.


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Red Army gets by Extreme, 6-4

Extreme forward Chelan Hein fends off the Red Army’s Erin Murdoch during Saturday’s game.  - James Clarke photo
Extreme forward Chelan Hein fends off the Red Army’s Erin Murdoch during Saturday’s game.
— image credit: James Clarke photo

Oceanside’s Midget C Red Army boys team trimmed the Mid-Island Extreme Bantam Rep girls 6-4 Saturday night at Victor Kraatz Arena in a fast-paced hockey game big on effort and nice plays.

“The kids are skating tonight,” chuckled one dad from behind the glass.

“Good game, eh? Our guys worked hard to beat them,” Red Army coach Bill Collette said after, adding their short bench made for a big challenge, and that “we were very impressed with those girls; they could really skate.”

Red Army was on the attack early and led 2-0 after the first period. Both teams scored one in the second and added three more each in the third.

“It was a good game,” Extreme manager Tina McComb agreed after, adding the season is young and Saturday was an exhibition game.

“They started out a bit slow, but they picked it up.”

Tina’s daughter Celine Tardif is in her first year of Midget (she played with the Bantam Extreme last year) and is one of three local girls on the Extreme’s roster of 15. Fellow forward Tianna Squire and goalie Maddy Meyer the other two.

“They’re used to it,” Tina confirmed when asked if the girls seemed not only unfazed by the size differential between them and most of their counterparts on the ice — the Red Army also pencils in a few female players — but fearless.

The Extreme’s main competition is the South Island Breakers (who they beat 3-1 in exhibition two weeks ago), but when not playing the Breakers, either in exhibition or in provincial qualifying action, they take to the ice against Midget boys teams.

We encourage an open exchange of ideas on this story's topic, but we ask you to follow our guidelines for respecting community standards. Personal attacks, inappropriate language, and off-topic comments may be removed, and comment privileges revoked, per our Terms of Use. Please see our FAQ if you have questions or concerns about using Facebook to comment.

Community Events, October 2016

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Karlovy Vary Spas

Much like the rest of Europe, the citizens of Prague realized some time ago that the waters of mineral springs can be used to enhance the health of those bathing in them. By the late 1700s, spas had become a fashionable place for the rich to be seen in, as well as somewhere where they could attempt to cure various maladies.

Spas still remain an extremely popular destination for Czechs, as well as for those visiting from Germany and other Eastern European countries. This is seen most in the Spa Triangle, which is made up of Karlovy Vary, Marianske Lazne and Frantiskovy Lazne. Visitors generally stay for a number of days and follow a specially designed program tailored to their needs, which some of the treatments including mud baths and the imbibing of mineral water.

The spa process is not as relaxing as many people think though, as the regiment can seen rather on the clinical side. Those expecting to sit in a hot tub with a glass of champagne will find that this is not the case. More realistically, the process involves a number of staff members shunting you from one treatment to the other, without time to relax in between.

karlovy_vary01.jpgOne of the most luxurious spas is the Zamecky Lazne – the Castle Spa – and the web address for this establishment is carlsbad-plaza.com. This place has seven different saunas, as well as aromatherapy, reflexology and even oxygen therapy.

Lazne III (www.lazneiii.cz) is another popular destination, although this is a less relaxing option. The staff here administer treatments at an unrelenting pace, even including colonic irrigation!

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How do I get there?

Fly into Santiago de los Caballeros airport (STI). All flights from the DC area are one-stop routes and take between 6-9 hours depending on layovers. Delta and United have the best routes with average prices from BWI and DCA around $550 for an economy-class ticket. From the NYC area, non-stop flights average $450. The cost of airfare will rise, as the departure date gets closer.

*DO NOT book your flight prior to contacting Pravassa to confirm the tour is happening.

Arrival/Departure Information

Check-in begins at 3pm on the start date of our tour. A designated group airport transfer is included in your package, and will depart early afternoon on arrival day. Please make sure Pravassa has your flight itinerary so we can schedule your transfer. Our program will begin on arrival day with a welcome introduction followed by dinner.

Our group departure for STI will leave the hotel around 10am on our final day. If you have an early morning departure we can arrange a private transfer for you payable to the driver. We will confirm our group airport transfers once we have travel plans from the group attendees.

  • The national language of the DR is Spanish. The resort staff and tour guides will speak English, but most of the locals will not. As a conscious traveler we suggest you download a translation app such as Google Translate and learn hello, good morning, please and thank you.
  • The currency is the Dominican Peso with the current exchange rate at $1 US = 45 Pesos. This number can fluctuate, so we suggest downloading a currency app to your phone for quick conversions. Cash is King for small purchases such as tips, dining in neighborhood restaurants and massages - so be prepared. There is NO NEED to get Pesos before you arrive in the DR as everyone accepts USD. Prior to departure we will advise you on the best amount of spending cash to bring with you.
  • Visa or Mastercard is accepted for larger purchases. Call your credit card companies before you leave home and inform them of your trip. There's nothing worse than being stuck overseas with no access to money!
  • Here's a sample of what you can expect to spend:
    • Bottle of Water - $1USD
    • Cappuccino - $2.5USD
    • Beer - $2.5USD
    • Kayaking tour - $45USD
  • Tipping for outside offerings is voluntary and typically 10% for restaurants, spa treatments, and taxis.
  • Electricity is the same as in the US so no need to worry about bringing anything special.
  • Drinking filtered or bottled water is your best bet to stay healthy. It's cheap and available everywhere.
  • The DR is 1 hours ahead of EST during the time of our visit.
  • Taxes & Services charges are included in your Pravassa package.

Travel Info

All traveler's passports MUST be valid for 6 months after date of entry into Dominican Republic. Please make sure to check your passport at least two months before your departure so you have time to update it if necessary. For US Citizens there is a $10 on arrival Visa fee to be paid.

For our week long trip you will only need casual clothing. You'll receive a full packing list, in our exclusive Wellness Travel Guide, before we depart, but to get started think about getting a small bottle of laundry soap to wash your clothes in the sink, pack light weight clothing, a yoga mat & props, yoga clothes, a bathing suit, ear plugs and a good pair of hiking shoes if you want to explore.

Fitness Level

Please keep in mind that this is your vacation and you have the freedom to skip any wellness activity that does not resonate with you.

Our yoga classes in the Dominican Republic will be an open-air room with ceiling fans. It can be hot and humid so be prepared to practice in these conditions. Classes will be a mixture of flow asana, meditation, and movement designed to encourage rejuvenation, healing, and rest, while at the same time creating heart openings, empowering, and stimulating you both physically and mentally. Our instructors, Moses Brown and Bita Jenkins, will customize their instructions to every fitness level. Beginners are always welcome.

Swimming/National Park

We'll be in the middle of a national park so rugged terrain will literally be at your feet. We suggest you bring sneakers with good treads and/or water shoes so you can wade out into the ocean. If you plan to be out for more than an hour, bring a day-pack so you can store extra water and sunscreen. Bikes are available to borrow at the hotel so you can also explore the salt-works area where Morton's cultivates their supply or ride to a local restaurant for lunch.

Health Concerns and Medical Emergencies

Pravassa will be purchasing a group travel protection plan for our travelers to help protect you, your trip, and your investment. The Plan Document, with full coverage details will be sent to you upon registration. Even if you're healthy, accidents can happen! Our group protection plan covers things such trip delay, lost luggage, accidents and emergencies.

Stomach problems can be common when traveling. Drinking bottled or filtered water is the best way to stay healthy. We suggest you pack activated charcoal or oil of oregano to settle your stomach should it become upset.

We will be visiting a remote area of the Dominican Republic where the locals are friendly and welcoming. While pickpocketing is prevalent around the world, if you are aware of your surroundings you'll have nothing to worry about. Please do not leave your belongings unattended at the beach and when off-property we suggest women dress respectfully in long pants or long skirts.

Climate and Weather

The Dominican Republic has that perfect Caribbean weather. Days will average around 85° with the possibility of a mid-afternoon tropical shower. Temperatures can cool at night to a breezy 65° so we suggest packing a loose cotton clothing, lots of sunscreen and some layers for sunset.

What will I eat?

Our trip will be based on a pescatarian offering (fish and vegetables). We have hired a wonderful chef and nutritionist who specializes in Caribbean cuisine to plan our menus for the week. The region offers a variety of freshly grown herbs and local ingredients from the sea and its rivers, including shrimp, lobster, octopus, grouper, snapper, mullet, tilapia, and crab. Fresh fruit from bananas to pineapples and guava to passion fruit will keep you hydrated and happy. For breakfast, we'll make sure you have a variety of healthy options and traditional foods to start your day. Such as coffee, fruit and yogurt.

We can accommodate gluten-free allergies and other dietary concerns if we know about them in advance.

What is NOT included in the vacation price?

International airfare, alcohol, any additional activities such as day tours, car rental, spa treatments, and personal expenses, which are not included in the package price.

Cell Phone & Internet Service

Our hotel has Wifi access, but please remember we visiting a remote area and cannot guarantee the connection. We ask that you download any movies, TV shows before you arrive so you do not monopolize the connection. We ask that you not bring your phone to our group dinners so that we may enjoy a daily digital detox. To keep in contact with home, use social media or download calling apps such as Skype, Viber, or What's App.

What else can I do while in the DR?

Think of our time together as the perfect opportunity to enjoy a digital detox and get out in nature. Water sports such as kayaking and snorkeling are popular as is hiking the wild west terrain. Spend your free time at the pool or beach or borrow a bicycle and ride 4 miles into the small town to explore.

How Shall I Prepare?

Dominican Republic Info

Articles & blogs on the area can be found via this travel guide and this national newspaper.

Dominican author Julia Alvarez has written many a love letter to her home country. Check out her works before you leave.

Love the spices in Dominican food? This handy guide dives deep into the cultural cuisine.

Baseball and the Dominican Republic go hand-in-hand. This tale follows "Sugar" through this journey.


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Thursday, July 7, 2016

Flights Cancelled July 8, 2016 due to Typhoon Butchoy

MANILA, Philippines - Some of the flights in Ninoy Aquino International Airport (NAIA), Manila have been cancelled due to heavy rains brought about by Typhoon "Butchoy". As reported, these are the flights affected, courtesy of GMANews:

Domestic Flights

  • PAL Express: 2P 2084 and 2085 Manila to Basco and Basco to Manila
  • SkyJet: M8 816 and 817 Manila to Basco and Basco to Manila
International Flight
  • China Airlines (CI) 711 & 712: Manila-Kaohsiung (Taiwan) and Kaohsiung (Taiwan) to Manila
As reported, passengers may contact Manila International Airport Authority Public Affairs Department for inquiries. Below are the contact numbers:
  • +632) 877-1109 local 4229
  • (+632) 854-0015
  • (+639) 99902-2488
Meanwhile, works and classes in public and private schools were also suspended July 8, 2016 in major parts of Manila area due to heavy rains.

SEE ALSO: #WALANG PASOK: No Classes Due to Typhoon Butchoy July 8, 2016

As of 4:00 AM update of PAGASA, Typhoon "Butchoy" which is currently heading Taiwan (current forecast track) has a maximum sustained winds of 195 kph near the center and gustiness of up to 230 kph.

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News ·

Gathering Of The Juggalos 2012 Lineup Announced

It’s that time of the year again folks you’ve all been waiting for. If you guessed that it’s time to announce to the lineup for the Gathering of the Juggalos, then you win. The annual festival put together by the Insane Clown Posse will once again have one of the most bizarre, yet eclectic lineups out there.

Taking place August 8-12 in Cave-In-Rock, IL, this year’s edition will feature ICP and their Psychopathic Records posse, a strong list of rappers (a pretty stellar list), every B-list late-’90s/early-’00s nu-metal band will play, aging comedian will be there as well.  And yes, it wouldn’t be a Gathering Of The Juggalos if there weren’t wrestlers at the event. See the music lineup below and check out more acts on the festival website:

Insane Clown Posse

Geto Boys

Master P

The Game

Danny Brown

George Clinton and Parliament Funkadelic

The Pharcyde

Tech N9ne

Kool Keith

Project Pat

Mack 10

Warren G

The Fat Boys

Biz Markie

Fear Factory

(Hed) PE

Kottonmouth Kings

And watch the infomerical below:

Adult Swim, The Field - Listen: The Field, "Comenius Garden" DOOM, Masta Ace Stream Masta Ace & DOOM's Collab Album, 'Son Of Yvonne'
Gathering Of The Juggalos
Insane Clown Posse

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Welcome to Our Practice

Stephen Sudderth, MD, FACS, is the ONLY physician in the State of Mississippi to obtain Board Certification in the core treatment of obesity serving Central and Southern MS . Dr. Sudderth was named by the American Board of Bariatric Medicine (ABBM) as the second physician in the State of Mississippi to receive the highest certification of achievement available in the United States. There are only 404 physicians nationwide who have received this designation. Dr. Stephen Stephen is also a board certified, recertified general surgeon and active member of the Fellow of the American College of Surgeons. Dr. Sudderth is also a Vista Certified bariatric surgeon.

Dr. Stephen Sudderth partners with each of our patients to develop a treatment plan tailored to each patient's individual needs, focusing on weight loss and reducing the health risks associated with excess weight. Whether a patient wants to lose several hundred pounds or just a few, our comprehensive approach will help all of our patients to establish long-term goals to avoid endless cycles of yo-yo weight loss and weight gain. Dr. Sudderth’s partnership with each patient to combat the chronic illness of obesity begins with the patient and ultimately perpetuates into the household. Because of his personal stake in each patient’s success, his efforts can be far reaching beyond the patient. “Obesity is something that we must address from every facet of the family dynamic. Yes, this is an issue for the individual patient but we have to change the environment where that patient lives and eats, in order to breed success and make a lasting lifestyle change,” said Sudderth. “I look at the big picture and I expect my patients to do their part, so that together we can achieve a healthier approach for their weight loss and goal achievements.”

We Offer:

  • Customized Weight Management Program for Each Patient
  • Medically Supervised  Weight Loss Under a Board Certified Physician
  • Access to our Clinical Dietician
  • Dietary Meal Planning Based on Each Patient's Likes and Dislikes of Food
  • Body Composition Analysis
  • Comprehensive Maintenance Program Tailored for Each Patient
  • Professional Staff to Partner With Each Patient to Attain Weight Loss and Health Goals

We can help you make the change...

Call our office to schedule an appointment.

Our Team of Experts Go

Your Weight is Over!


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 Car Repair and Towing Service | Poughkeepsie, NY | Prestige Automotive
Prestige Automotive Repair and TowingPrestige Automotive Repair and TowingCar, Motorcycle ot Truck Towing Service, PoughkeepsieAuto Car Repair Service, PoughkeepsieFree Auto Car Repair Quote, PoughkeepsieContact Prestige Automotive
The Prestige Difference
  • Honest Service
  • Friendly Mechanics
  • Gas Station and Store
  • NY State Certified Inspection
  • Local & Long Distance Towing

Call (845) 462-7373 for a quote or stop by the shop.

We're located at 2 Vassar Road, Poughkeepsie, NY 12603.

Established in 1983

Welcome to the website of Prestige Automotive & Towing, the home of Poughkeepsie’s best prices on auto repair and tow services. We offer quality service at competitive prices. Your satisfaction is our Number 1 goal.

When your vehicle needs service, you want it fixed quickly, reliably, and affordably. We understand. If your car won't start, we're here for you. If you break down away from home, we're here for you. If you hear a weird noise and want it diagnosed, we're here for you.

Prestige Automotive & Towing, we've been in business at our 2 Vassar Road location here in Poughkeepsie, NY for almost three decades. That says something! We work hard to ensure that all of our customers receive top quality service at reasonable rates.

Car, Motorcycle ot Truck Towing Service, Poughkeepsie

Free Accident Tip Sheet

ASE Certified Mechanic  Better Business Bureau  Dutchess County Chamber of Commerce (Poughkeepsie, Mid-Hudson)

Call (845) 462-7373

Ask about our local and long distance towing.

When you see the Prestige tow truck, you know your car will be handled with care.

We're there when you need us

With our convenient location in Red Oaks Mills, we provide fast service to all the major institutions and locations in Poughkeepsie including: stores, shops and restaurants along Route 9, the Poughkeepsie Galleria Mall, the South Hills Mall, Marist College, Vassar College, Vassar Hospital, St. Francis Hospital, and IBM.

We are happy to travel throughout the Hudson Valley. Look for our tow trucks up and down Rte 9 covering accidents and providing roadside assistance in Fishkill, Wappinger Falls, Poughkeepsie, Hyde Park and Rhinebeck. Prestige also works in Hopewell Junction, Lagrangeville and other areas in Dutchess County.

Home | Contact | Quote | Services | Towing

(845) 462-7373
©2010 Prestige Automotive | Designed by Imagination Farm


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Fuoco incrociato: General Romero, leader of a small Latin American country, is showing the UN how he's taking care of his country's drug problems by publicly burning crops of marijuana. As soon as the inspectors leave, however, Romero quickly returns to business, producing and shipping the illegal crop. When his right-hand man is convinced by a crusading reporter to turn away from his life of crime and help bring his boss to justice, Romero vows to stop at nothing to prevent this traitor from talking.

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Director: Alfonso Brescia
Actors: Carlos Santos Maurice Poli Peter Hintz Riccardo Petrazzi

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Streaming films is becoming more risky every day. We strongly urge our users to use a VPN to make themselves anonymous while streaming films and TV shows online. Using a VPN ensures that your ISP, the government and the NSA can’t spy on you or block any streams. They will not be able to see ANY of your online activity. Protect Your Privacy with a Free VPN
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PIANA Triangle bikini top Clockwork orange

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Saratoga County, New York

According to the 2000 Census, Saratoga County, New York has a population of 200,635 people. Of those, 192,579 (96%) are White, 2,725 (1%) are Black, and 2,834 (1%) are Latino[1]. However, 1,058 (or 1% of the 200,635 people) are not residents by choice but are people in prison.

Even though prisoners cannot participate in the local community, the Census Bureau nevertheless counts them as residents of the county where they are incarcerated.

A more accurate description would not include the prisoners. This would give Saratoga County a population of 199,577 with a demographic that is 96% White, 1% Black, and 1% Latino.

Reported in
Census 2000
Total 200,635 1,058 199,577
White 192,579 425 192,154
Black 2,725 486 2,239
Latino 2,834 234 2,600

[1]The numbers for Whites, Blacks and Latinos may not add up to the total number because we have not included racial groups other than Whites and Blacks and because the Census Bureau considers "Latino" to be an ethnicity, not a race. Most of the people reported as being Latino are also counted as being White or Black.

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Active Duty Troops In Iraq Are Wearing Oath Keeper Tabs: “… what we are wearing while over here in Iraq”

  • Print The Alex Jones Channel Alex Jones Show podcast Prison Planet TV Infowars.com Twitter Alex Jones' Facebook Infowars store

Oath Keepers
Friday, October 9, 2009

A Three Percent Oath Keeper in Iraq

The above photo was submitted by an active duty Oath Keeper in Mosul Iraq, sporting an Oath Keepers tab.   As his other tab and patch make clear, he is also a “Three Percenter.”  (see below)

The photo was also posted over at the Sipsey Street Irregulars blog with this message:

Just thought the WRSA [Western Rifle Shooters Association] and the Sipsey Street boys might like to see what we are wearing while over here in Iraq.  We . . . know what the deal is, and when the time comes (which it will), we know where we stand and continue to make preparations for it.

Keep up the good work!

Regards from Mosul,

All I can say to that is Hooah!  Thank you for your service, K7C, and for stepping up.  Give our best regards to your brothers in arms, and let them know we have your six back home.  If any of you need anything at all, from socks to helmets, to body armor, just ask and it will be provided.   We need you to stay safe and come home healthy.

Oath Keepers here stateside, let your brothers on active duty in Iraq know how you feel.  They do come here to read comments, count on that.

  • A d v e r t i s e m e n t

Note that the tab in the photo is not the same tab we sell here on our site (which is more of a police style tab).  We are having military ACU style tabs with velcro made up, but they are not yet finished.  That means the troops are having their own Oath Keeper tabs made up!  And no, we did not put them up to it.   They are doing this on their own initiative.

So take heart!  The message of the oath and its obligations is spreading, and the Guardians of the Republic are listening and responding.   And every time you act to spread the message, by whatever means, you cannot know what impact you will have, or how far it will reach.  That’s why it’s so crucial that we each do what we can to reach, teach, and inspire as many active duty as possible about their obligation to defend the Constitution and their duty to refuse unlawful orders that would violate the rights of their fellow Americans.

Upcoming Outreach Effort to Put Tabs, DVD’s, and OK Handbooks in the Hands of Active Duty

Coincidentally, the tabs those troops are wearing are almost exactly like the tabs we are having made for our upcoming care package initiative, which will put an Oath Keeper tab, DVD, Oath Keepers handbook, copy of the Declaration of Independence and Constitution, and other outreach materials, into the hands of active duty deployed worldwide.  We plan on launching that effort on Veteran’s Day, November 11 and continuing right on through Bill of Rights Day, December 15, with the goal of delivering all the care packages by Christmas.  Now imagine what that will be like!  Tens of thousands of our troops reached with the message and a tab.  I will post more details very soon.

What’s a Three Percenter?

Now, seeing the other tab and patch in the photo, you may ask “what is a three percenter?”  The narrowest definition is that three percenters are hard-line gun owners who are done backing up and will not comply with more infringements of their right to bear arms.   A broader definition would be that they are hard-line Americans who are done backing up and will not comply with further  infringement of any of their rights.  It also alludes to the three percent of the colonists who took to the field against the King during the Revolution, and the estimation that at least three percent of modern Americans will actively fight, if it comes to it, to preserve liberty.  Read this essay for more explanation.

Oath Keepers and Three Percenters are separate groups, but it is not a surprise to see people who consider themselves both an Oath Keeper and a Three Percenter. If you read the supplied links you will see why.

While we Oath Keepers have a specialized mission of outreach to current serving, focused on the oath and on refusing to obey unlawful orders, there’s lots of common ground and shared commitment to the Republic among both groups.   Go here to read what the Sipsey Street blog has to say about that.

Oath Keepers, expect to see more of these tabs popping up soon, even before we begin our care package initiative.  You are making a real difference.   Keep up the good work!

For the Republic,

Stewart Rhodes

This article was posted: Friday, October 9, 2009 at 10:16 am

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Hillary Called Black Servant the 'N-Word' Claims Former Clinton Chef See the rest on the Alex Jones YouTube channel.

Stupid Hipster Hillary Millennials See the rest on the Alex Jones YouTube channel.

http://www.youtube.com/embed/PQkm4MtGNAQ http://www.youtube.com/embed/3Jva3c7mVvc

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Al-Qaeda’s Seat at UN, American Riots & More: Infowars Nightly News

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Infowars Nightly News
Sept 18, 2011

– Following David Cameron and Nicolas Sarkozy’s disgusting act of grandstanding yesterday, Al-Qaeda, otherwise known as the Libyan rebel army, is being given its own seat on the UN. Alex explains why this represents the ultimate statement of hypocrisy.

– Scientists have announced experiments will be undertaken geared towards the ultimate goal of injecting the upper atmosphere with sulphuric particles in a bid to ‘prevent global warming’. Alex will document how geoengineering is the public face of an admitted program to reduce global population by bombarding the public with aerosols through chemtrails and other high-altitude biological toxins.

– Three years after Alex Jones and the likes of Gerald Celente first predicted that the economic recession would cause riots, Mayor Bloomberg has now publicly stated that chaos will hit American streets if more jobs are not created. We were way ahead of this curve on this but now even establishment figures and the mainstream media are forced to admit that scenes already witnessed in the UK, across Europe and the Middle East are now on the verge of being replicated in the United States.

Alex will also discuss the continuing cover-up regarding Swissair Flight 111, Ralf Nader’s statements about America being under a two party dictatorship, as well as moves by Tucson city council to abolish free speech.

You will also have the opportunity to watch the best of the last 2 weeks of Infowars Nightly News, including guest segments, man on the street reports, and the most important news covered so far.

This article was posted: Sunday, September 18, 2011 at 5:54 am

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Hillary Called Black Servant the 'N-Word' Claims Former Clinton Chef See the rest on the Alex Jones YouTube channel.

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Stiglitz Says U.S. Recession ‘Nowhere Near’ End After GDP Jump

Bob Willis
Bloomberg [1]
Sunday, Nov 1st, 2009

Nobel Prize-winning economist Joseph E. Stiglitz said the U.S. recession is “nowhere near” an end and the economy’s third-quarter growth rate of 3.5 percent, the first expansion in more than a year, won’t carry into 2010.

While this week’s figures on gross domestic product are “very good,” the numbers would be “miserable” without stimulus measures enacted by the Obama administration, Stiglitz said today at a forum in Shanghai. He urged the U.S. and other countries not to pull back on efforts to shore up economies.

“When we look at if workers can get jobs, if they can work full time, if businesses are able to sell goods they produce, in those terms, we are nowhere near the end of recession” in the U.S., said Stiglitz, 66, the former chief economist at the World Bank. The U.S. job market is still “in very bad shape.”

Federal assistance to the housing and auto industries helped propel growth in the July-September quarter. President Barack Obama, in his weekly radio address to the nation, today called the Oct. 29 report on GDP a “good sign” and said an expanding economy is the first step to job creation.

Full article here [1]


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Seagate Christian School

1010 Whippoorwill Lane
Naples, FL 34105-0339
Tel: (239)262-2226
Grades: 5-8 | 5 students
Founded in 1976, the Seagate Christian School exists to give the young people of our church and community bible based education while training them to develop the mind of Christ.
SCS is in its 35th year of operation and stands in our community as a wonderful place to educate the entire child, academically, spiritually, socially and physically.

School Overview

Religious Affiliation Baptist
Grades Offered Grades 5-8

Student Body

Total Students 5 students
Student Body Type Co-ed
Students by Grade Seagate Christian School Student By Grade

Academics and Faculty

Total Classroom Teachers 8 teachers
Student : Teacher Ratio 1:1
National avg.: 13:1
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People that viewed Seagate Christian School also viewed these schools: Seacrest Country Day School, The Village School, First Baptist Academy, Community School Of Naples and Naples Christian Academy.
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  • Naples
    Grades: NS-12 | 562 students
    5.2 miles
    7100 Davis Boulevard
    Naples, FL 34104
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    Grades: PK-9 | 551 students
    3.2 miles
    6000 Goodlette Rd N
    Naples, FL 34109
  • Naples
    Grades: PK-12 | 562 students
    1.9 miles
    3000 Orange Blossom Drive
    Naples, FL 34109
  • Naples
    Grades: NS-12 | 716 students
    0.8 miles
    13275 Livingston Road
    Naples, FL 34109
  • Naples
    Grades: PK-8 | 158 students
    3.2 miles
    3161 Santa Barbara Blvd
    Naples, FL 34116

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1000+ Economists, 15 Nobel Laureates, Endorse Bipartisan INFORM Act

Full-page Open Letter Runs in the New York Times

Oct 22, 2013, 12:10 ET from The Can Kicks Back

WASHINGTON, Oct. 22, 2013 /PRNewswire-USNewswire/ -- The Can Kicks Back (TCKB), a non-partisan and Millennial-led campaign to mobilize young Americans on the issues of the national debt and generational equity, today announced the support of over 1,000 economists—and 15 Nobel Laureates—for the bipartisan Intergenerational Financial Obligations Reform (INFORM) Act. Championed by TCKB and introduced in the House and Senate by members of both major parties, the INFORM Act would disclose the true size and intergenerational consequences of our country's fiscal imbalance. It will do so by incorporating fiscal gap and generational accounting analysis in the budget process.

"I've never seen such massive support by the economics profession, independent of political leaning, for any piece of legislation," said Laurence Kotlikoff, professor of economics at Boston University, who helped spearhead the development of generational accounting.

In addition to the 15 Nobel Laureates, prominent endorsers include George Schultz, former U.S. Treasury Secretary, David M. Walker, former U.S. Comptroller General, and James C. Miller, former Director of the Office of Management and Budget.  The INFORM Act's congressional co-sponsors include Senator Tim Kaine (D–Va.), Senator John Thune (R–S.D.), Representative Jim Cooper (D–Tenn.) and Representative Aaron Schock (R–Ill.).

A full-page ad in the New York Times today features an open letter to the president and Congress urging passage of the INFORM Act. The letter reads, "Unless we begin to systematically measure and address our long-term fiscal imbalances, no government programs, be they maintaining our nation's military or paying Social Security benefits, will be secure."

Over 15,000 millennials have also endorsed the INFORM Act through a petition circulated online and through a nationwide tour organized by TCKB. The campaign is planning a rally and press conference on Capitol Hill on Thursday, October 31st to deliver these petitions and other messages from young people to members of Congress.

"The millennial generation must fight for our future," said Nick Troiano, co-founder of TCKB. "The INFORM Act is the first step toward addressing our country's fiscal challenges that threaten to leave millennials as the first generation worse off than our parents."

The Can Kicks Back is a non-partisan, Millennial-driven campaign to fix the national debt and reclaim our American Dream.  More information is available at www.TheCanKicksBack.org/informact.

Contact: Bill Beaman
Email: press@thecankicksback.org
Mobile: 301-767-7807

SOURCE The Can Kicks Back


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Canadian Manufacturers See Reasons for Optimism

Considering the soaring Canadian dollar, Statistics Canada decided to poll Canadian manufacturers to see just how much pain they are in over it. Oddly enough, although they are undeniably hurting, manufacturers appear to have reasons for optimism.

The next logical question is, why? Apparently, although it may be harder to sell their stuff and more expensive to get their hands on raw materials, Canadian manufacturers are confident when it comes to strong production and even stronger employment in the sector.

Just in case you don’t think manufacturers have completely lost their marbles, they are aware that in the months ahead, their ride will be nothing but smooth. For starters, the first point of concern is the declining rates of new orders, as well as lower-than-normal levels of unfilled orders. According to Stats Canada, 19% of polled companies expect new orders to decline in the third quarter. This percentage is considerably higher than 11% reported for the second quarter. Furthermore, 18% of those surveyed reported lower-than- expected levels of unfilled orders, in comparison to 16% reported for the previous quarter.

Still, when Stats Canada’s pollsters balanced optimists versus pessimists in relation to production levels, employment, and business impediments, at least more are expecting improvements in the next business cycle, rather than forecasting a downturn.

What does that mean to investors? I’m sure you’ll agree that the past few trading sessions were difficult to ignore. The stock markets took quite a few hard hits. Our largest trade partner south of the border is having a choppy ride, to put it mildly, mostly fueled by the troubled credit market bubble that seems just about ready to burst and wreak its special kind of havoc. On the other hand, U.S. businesses are hopeful when it comes to earnings and employment.

As long as this balance of opinions holds, and as long as the U.S. credit market bubble bursts, it is not too strongly reminiscent of what happened when the tech bubble burst in 2000. Canadian manufacturers will have buyers for their products and will be able not only to stay afloat, but actually to get ahead. Having buyers means potentially more new orders and lower inventories, which means more revenues and stronger earnings.

Even though optimism is intangible, cannot be measured in cash, and is often prone to overshooting, it is the necessary factor that often gives the incentive to push harder and go that extra proverbial mile, pardon the cliché. So when we talk about markets getting jittery, it means there are more pessimists than optimists. And when we talk about markets rallying, it means there are more optimists than pessimists. The way things are going right now, North American markets need as many optimists as possible, and badly!


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Bill Longstaff: The windmill next door

As a citizen of Alberta, Canada’s oil province and the birthplace of wind energy in Canada, I have perhaps less innate concern about windmills than citizens from other provinces. Indeed, the first time I walked up to a windmill, I was favourably impressed. Although I found its size somewhat intimidating, I found the rhythmic swish . . . → Read More: Bill Longstaff: The windmill next door


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Get Her Look: Classic Beauty

Makeup artist Danika Lamb dishes on the products and techniques she used to create bride Angela's classic beauty look. Like what you see? Read on to learn how to recreate it!

classic wedding makeup by artistry by danika

Photo by Braedon Flynn

Prep Time: Exfoliate!
Use a gentle exfoliant the night before your wedding to ensure a smooth and flawless makeup application. love is Kate Somerville's ExfoliKate Gentle Exfolianting Treatment ($65).  It's gentle yet very effective!
Primer & Foundation
I primed Angela's face with Temptu's primer ($12 for 1 oz.) and then airbrushed her skin with Temptu's SB foundation in number 3 ($27.50).  If you aren't able to airbrush, I suggest using Armani Luminous Silk foundation ($60) and primer ($57).
All About The Eyes
Your eyes should really "pop" on your wedding day!  One way to do this is by adding false lashes.  On this particular bride I used Ardell strip lashes 120 ($11.03) to give her that gorgeous wide-eyed look. Be sure to trim the false lashes before applying so that they don't hang past your natural lash line. All the eyeshadows I used come in Too Faced Cosmetic's The Natural Eye palette ($32.40).  This is a very flattering palette for most skin tones and comes with fun instructions for different eye looks. From this palette I used 'Silk Teddy'  from lash to brow and 'Cocoa Puff' in the crease. I then lined and then lined the eyes with 'Sexspresso'.
Lips & Cheeks
I applied MAC blush in 'Pinch O' Peach' ($25.64) to the apples of Angela's cheeks.  To complete her look I used Sonia Kashuk's Velvety Matte Lip Crayon in Pinky Nude 09 ($7.59) to line and fill in her lips.  To give the lips a hint of shine, I  dotted on a bit of Sephora's Glossy Gloss in Pink Macaroon 11 ($12).

More Wedding Beauty Inspiration: Bold Lips | Our Favorite Bridal Beauty Looks | Long Hair Ideas
Last Updated: August 11, 2013 at 1:35 am
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 PromiseAngels: Horace Bushnell - Christian Nurture - [Item: 116764 - ISBN: 1579104487] - Promise Angels Bookstore
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Christian Nurture [Paperback]

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Purchase of this book includes free trial access to www.million-books.com where you can read more than a million books for free. This is an OCR edition with typos. Excerpt from book: of the world. Neither let your own experience raise a prejudice against it. If you have endeavored to realize the very truth I here affirm, but find that your children do not exhibit the character you have looked for; if they seem to be intractable to religious influences, and sometimes to display an apparent aversion to the very subject of religion itself, you are not of course to conclude that the doctrine I here maintain is untrue or impracticable. You may be unreasonable in your expectations of your children. Possibly, there may be seeds of holy principle in them, which you do not discover. A child acts out his present feelings, the feelings of the moment, without qualification or disguise. And how, many times, would all you appear, if you were to do the same? Will you expect of them to be better, and more constant and consistent, than yourselves; or will you rather expect them to be children, human children still, living a mixed life, trying out the good and evil of the world, and preparing, as older Christians do, when they have taken a lesson of sorrow and emptiness, to turn again to the true good ? Perhaps they will go through a rough mental struggle, at some future day, and seem, to others and to themselves, there to have entered on a Christian life. And yet it may be true that there was still some root of right principle established in their childhood, which is here only quickened and developed, as when Christians of a mature age are revived in their piety, after a period of spiritual lethargy; for it is conceivable that regenerate character may exist, long before it is fully and formally developed. But suppose there is really no trace or seed of holy principle in your children, has there been no fault of piety and chapter{Section 4constancy in your church ? ...

Promise Angels is dedicated to bringing you great books at great prices. Whether you read for entertainment, to learn, or for literacy - you will find what you want at promiseangels.com!

Item Specifications...

Studio: Wipf & Stock
Pages   408
Est. Packaging Dimensions:   Length: 7.54" Width: 5.19" Height: 0.94"
Weight:   0.86 lbs.
Binding  Softcover
Release Date   Jun 1, 2001
Publisher   Wipf & Stock Publishers
ISBN  1579104487
ISBN13  9781579104481

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Marvel Arco Marvel Realtors - Pune

Rs.88.08 Lacs-1.76 Cr
  • Location : Magarpatta Road, Pune
  • Total Project Area:6 Acres
  • Total No. Of Buildings:6
  • Builder:Marvel Realtors
  • Type:2, 3, 3.5 BHK Apartments
  • Project Status :Ready Possession
  • Size:1355 Sq ft. to 2700 Sq ft.
  • Total Stories: 12
  • Total Flats: 228

Marvel Arco - Builder

Known for their high quality, elegant, and marvelous creations, the real estate professionals at Marvel Realtors have always been acclaimed highly by the home seekers as well as the real estate professionals. Its present project Marvel Fria at Wagholi, Pune is unarguably an opulent piece that exhibits the experience and innovativeness of the experts at Marvel Realtors. Whether it is the architectural magnificence, quality, elegance, technology, campus planning, amenities, or any other aspect concerning a perfect residential project; Marvel properties are the perfect choice for the home seekers desiring an opulent, luxurious, and peaceful life.

Since last eleven years, the experts at Marvel Realtors have proven their potentials of constructing the high quality and luxurious projects. Marvel Realtors is a recognized name in the real estate circles in and around Pune, but with their upcoming projects in Mumbai, Goa, and Bangalore etc; they are soon to widen the horizon of their success and popularity. The present project Marvel Fria is elegantly designed and developed. Enveloping the area of 26 acres, it embraces 13 buildings with 12 stories each. In total, it contains 611 apartments.Offering 2 BHK & 3 BHK luxurious apartments, it can duly cater to the needs of the people desiring to live a world class lifestyle.

For the people looking for high end and luxurious real estate properties in Wagholi, the Marvel Realtors stands as the top choice. Known for equipping their projects with highly sophisticated amenities, the experts at Marvel Realtors have decked the Marvel Fria with all the required amenities, such as, the aroma garden, basketball court, cricket pitch, club house, party lawn, multipurpose area, swimming pool, senior citizen park, tennis court, squash court, community garden, ecological garden, amphitheatre, barbeque area, river promenade, skating rink, trellis, yoga room, automatic lift, family lawn, and the visitor parking etc.

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Type Sizes (Sq ft.) Price / Sq ft. Basic Amount(Rs.)
2 BHK 1355 6500 Rs 88.08 Lacs * Onwards
2 BHK 1360 6500 Rs 88.4 Lacs * Onwards
2 BHK 1540 6500 Rs 1 Cr * Onwards
2 BHK (Penthouse) 2070 6500 Rs 1.35 Cr * Onwards
3 BHK 1835 6500 Rs 1.19 Cr * Onwards
3 BHK (Penthouse) 2700 6500 Rs 1.76 Cr * Onwards
3.5 BHK 2300 6500 Rs 1.5 Cr * Onwards

Location Advantage

  • Bapusaheb Magar Vasti Bus Stop - 1.3 Km
  • The Orbis School - 3.8 Km
  • Seasons Mall - 1.6 Km
  • Columbia Asia Hospital - Pune - 3.7 km
  • ICICI Bank ATM - 0.7 Km
  • Hadapsar Railway Station - 2.9 Km
  • Om Medicals - 1.6 Km
  • Bharat Petroleum - 2 Km

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For a number of people their residential home is a vital property that ensures their security financially. Hence a lot of people try their best to acquire homes and properties in some of the best region of the city which can prove to be valuable and secure for your future. There are several properties that are being undertaken in the city of pune and being the economic hub of the country, the demand for homes here is tremendous as more and more people come here for work and eventually settle down. Gagan Ela is one such property that has grabbed much attention from the

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Mantra Essence Undri Pune is A Perfect Residential Project Embellished with Contemporary Amenities

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Purchasing a property is a dream for many people. A dream is considered to be turned in reality, when you secure a perfect property deal. Now, the concept of perfect property is a little tricky. Many things are there to be considered. If you are looking for a property in Pune, you shall get some marvelous apartments in the posh regions of the city. Investing in these properties is always a smart choice. Nevertheless, these properties offer the best dwelling experiences to the property buyers. If you are looking for such a property, consider Mantra Essence to be your choice.

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Mantra Properties Mantra 24 West Gahunje Pune offers quality living

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24 West by Mantra is an excellent construction, superb planning, and lovely design will attract every real estate enthusiast desirous of possession of a luxurious abode that is complete with all modern amenities. It has been constructed taking care of the needs and requirements of varied needs of the urban population. To provide the prospective buyers with options 1 BHK and 2 BHK to select from with total of 600 apartments.

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ProSpec | Recombinant Protein Manufacturer
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Protein Catalog >> Enzymes >> Phosphatase >> DUT Pyrococcus Fruriosus
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Thermostable dUTPase Pyrococcus Fruriosus Recombinant
Ice Packs
Catalogue Number ENZ-281
Synonyms Thermostable dUTPase, dUTPase.
Description Thermostable dUTPase (pyrococcus fruriosus) maximizes the efficiency of high-fidelity PCR (using proofreading DNA polymerases). It removes contaminating dUTP present in PCR reactions and dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall and slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications such as cloning and subsequent recombinant protein technology, and gene expression analysis (semi-quantitative RT-PCR techniques and real-time PCR analysis), where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP and is critical for the fidelity of DNA replication and repair. dUTPase hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing dUTP levels and providing the dUMP for dTTP biosynthesis.
Source Escherichia Coli.
Physical Appearance Sterile filtered liquid formulation.
Formulation dUTPase is supplied in 20mM Tris-HCl (pH 8.2), 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.1% Nonidet P40, 0.1% Tween 20 and 50% glycerol at a concentration of 10U/µl of the enzyme.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity Greater than 97.0% as determined by SDS-PAGE.
Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
Activity (A) Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepes pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma.
(B) Enhancing PCR amplification: 50ul of Pfu PCR reaction system with 1-3u of dUTPase (<3.0kb) or 4-6u dUTPase (>3.0kb) to amplify genomic DNA target up to 15-19 kb in length. (High concentrations of dUTPase will inhibit PCR reaction!)
Specific Activity 10³u/ug.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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The Circuit ManMature

An aging underground tech trader makes the circuit of an exclusive open-air market.

The wind blew a slight chill through David's hair as he surveyed the strange scene before him. It always gathered in this meadow at noon, and he never quite knew what to expect of it. Small pockets of people moved through subtle paths in the long grass, the overcast day adding a fine finish of frost to the whole affair. David was partly a reporter, partly a survivor, and partly one of the crowd before him. The smallest open air market on the continent was slowly opening in front of him, groups of leather-clad bashers looking violent and shoving anyone anyone who wandered too close to their employers tables. The tables themselves were uniformly refurbished wood tables that propped up on steady legs and had a display covered in glass dominating the top with a small side drawer for the hiding of particularly prized secrets. David had spent a fair bit of time behind one of these tables in a decade past, meeting the right people at weird raves and waves, but now he merely observed and reported for a small digital publishing node. His editor, Gary, was sitting in his office right now hitting a vapo and waiting for a report. Gary was a middle-aged man with a ponytail and a stressed face, a girlfriend with a serious amphetamine habit who swallowed his money and jizz like water, and a passion for intellectual freedom acquired from his years on he other side as a corporate software raider that he would defend to his grave. A man who lived off the ad money of insane people who wanted to see copyright disbanded and the occasional large shipent of illegal parts. One of David’s best friends, the two had met at an after-rave of a crypto=conference. In some circles, the rest was history.

The underground merchants were both exclusive in their paranoid associations and the wares they presented. Illegal chips and scattered open-source projects that moved fast enough to avoid shutdown were the only boards that didn't have the NSA or the DIA hardwired into them these days. David picked out the flat grey of the Sleek uniform at a tabe, a few foreign faces from India and Malaysia, and a dozen friends/strangers eager to show off and impress. Obsession with secrecy due to the snooping eyes of the law had led to a range of cryptographic standards displayed in glowing neon that hung off the front of the tables  That segment of subculture  ranged from securely standard to esoteric languages that resembled a mix of computer code, binary, and a jumbled slew of music references shaken with obscure musical references. David had a few ciphers in his head himself, but he had gained membership here by his longevity. The gleaming circuit boards on display were illegal everywhere except three cities in Malaysia, and David remembered with a sinking feeling that there weren't many free cities left. He idly wandered from table to table, trading small talk about prices and import difficulties and who was still making music and who had died and the new Intel processors until something caught his eye. A small azure chip. He hadn't seen one of those in twelve years, and even back then, they were nigh legendary. Designed by a schizophrenic noise freak with a serious Hype habit, they were rumoured to distort electricity itself. He had seen them in action first hand in hacking houses it took favours and connections to get the address of. Once, he had seen a viral video of the inside of one, and it had looked like something simple enough to power a lightswitch with a few strange ripples across it. Nobody understood.

He drew in a breath imagining the possibilities. Decentralized banks. Small governments. A million nodes. Cracking into almost anything faster than the sane human mind could comprehend. David looked at the vendor, a small man in glaring retro-raver neon, a face that had not aged well due to the torrent of information and drugs, with small beady eyes that ferociously scanned David. The long greasy hair swept in front of his eyes, looking inherently unwashed. He idly wondered if this man had an idea of the value of the thing he possessed, but then decided that in these circles, of course he did. The true question was, what was the price? The two huge bashers to either side of the man were huge Ukranians, with the shaved heads and hard stares to commit unspeakable things with. Refugees of the wrong side of Eastern European fascism, no doubt, men who had spilled large amounts of blood. David felt his heart flutter for a single second, then kept his cool. He’d been in much worse situations. He calmly inquired, "What's the price for the azure WindBreaker?" The underground entrepreneur looked at him with a wide smile, a practically demonic grin, "The real name of someone you love." David pauses for a brief second, runs it through his mind. Fuck this, he could feel the grey in his hair. He could imagine the endless nights of guilt stretched upon him, the fate of someone dear destroyed by this vile soul who would make sure it happens, no compromise would be accepted by this man. No vague acquaintance, no good friend, someone he truly loved. While the man is trying to be a literal devil, he gives him a solid look. Dressed like an insane hallucinogenic general, flanked by ranking soldiers ready to do inhuman crimes, trying to be a cruel and vicious bargainer that will destroy his enemies, What a pretentious fuck.

David shakes his head slowly. As he refuses and turns, the neon merchant opens up his little side drawer and presses a button. The azure chip operates for a second, warps things a little, and a message is automatically relayed to David’s phone. When he checks it, the screams and anguish of hundreds who danced the dance and lost will be played at full volume and put on unstoppable replay and send until the phone is smashed. A combined practical joke and virus, a carefully curated collection, and it was truly heartbreaking to hear. David walks to the next table. A long day was ahead at the market, and his Sleek phone vibrating in the front pocket of his bomber jacket. He won’t even need to look at it to assume it’s Gary asking about margins and profits and whos-who of attendance. Still, in spite of all the insanity and his grey hair, David loved this part the best. The risk, the reward, the feeling of being on the cutting edge of the times, surfing all kinds of webs until he got to the right places. Walking in weird amazing places with strange intelligent people and something awful waiting to be played on his phone. In the field, he was a digital man of the times.

The End

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Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

Far western - (Mar/03/2005 )

blink.gif I'm searching someone who's a master in the technique of Far Western! I would like to test an interaction in vitro between two proteinn but I don't find the good conditions for it! If someone would have a good protocole? Thanks a lot tongue.gif

maybe may you have a look at thos web page :

or (used in scwartz and al. EMBOJ and guichet and al. Nat)

adapted at this webpage :

hope i've been helpful for a few.

hi margo!

you are searching for help with your far-western protocol, yes? Have you thought of using other systems to check for protein-protein interaction like yeast-two-hybrid, (co)-immunoprecipitation or (GST-) pulldowns?

if you could be a little more specific what your problem is, we might be able to give you some advice, maybe!

and please try to avoid double posting, though posting the same topic in two diffent languages is quite innovative! laugh.gif

Thanks answering!
For the double posting, I realized I didn't write in english but too late...
For the Far Western: The interaction I have to test has been shown in vivo by double hybrid system. I tried a co-IP system last year but no result with interaction (I have shown another one but not this interaction!) We think that Far Western would be a good argument because I use proteins expressed in human cells (cells extracts) and translated in vitro (better than the protein expressed in Coli and more or less purified).
Hope this informations will tell you something!


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Cascio Interstate Music Announces Annual Post-Summerfest Gear Sale on Musical Instruments

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Annual sale features gear used at The World's Largest Music Festival from the summer of 2013. It features savings of up to 70% on amps, drums and accessories at this Milwaukee music store.

Cascio Post-Summerfest Gear Sale

... these instruments were only used for the duration of Summerfest and have tons of use left in them ...

Cascio Interstate Music SuperStore, Wisconsin's largest music store in Greater Milwaukee, is offering the gear played by the stars of Summerfest 2013 with their Post-Summerfest Gear Sale. The annual Summerfest event on Milwaukee's lakeshore is known as The World's Largest Music Festival and brings the music industry’s hottest stars, emerging talent and local favorites with over 800 acts and over 1,000 performances, on 11 stages.

Cascio Interstate Music supports the musicians at the K-Nation/Cascio Stage and at other Summerfest stages with high quality musical instruments. And now that the festival is over, save up to 70% on over $400,000 worth of gear played by Summerfest performers like Imagine Dragons, Yeah Yeah Yeahs, Buddy Guy, Neon Trees, Phil Vassar, The Airborne Toxic Event, I'm Not A Pilot, Empire of the Sun, Dr. John, Alice Cooper, Don Henley and more.

Take advantage of great prices now on top brand music equipment such as:

  • DW Drums and Drum Accessories
  • Fender Amps and Speaker Cabinets
  • Gator Protechtor Percussion Cases
  • Hartke Bass Amplifiers and Bass Cabinets
  • Marshall Amps and Guitar Extension Cabinets
  • Vox Guitar Combo Amplifiers
  • Zildjian Cymbals

All of the used musical instruments provided for the musicians at Summerfest are heavily discounted for this Summerfest Gear Sale. Act quickly - these instruments were only used for the duration of Summerfest and have tons of use left in them. Get a great deal on lightly used musical instruments at this Milwaukee music store, and a piece of nostalgia from Summerfest, the World’s Largest Music Festival.

Cascio Interstate Music is a strong player in today's musical instruments retail industry, ranking in the top 12 nationally among music retailers, providing musical instruments and accessories to musicians of all ages, including music educators and music professionals worldwide. Cascio began in 1946 in West Milwaukee as a small teaching and accordion studio owned by Frank Cascio, the son of Italian immigrants. Today, over 75,000 musical products are marketed nationwide via catalogs and online, and locally at their SuperStore in New Berlin. Cascio also carries their own company-designed brands of guitars, drums and band instruments – Archer, Union and Ravel – for consumers and schools.

Cascio's involvement in the Milwaukee music community includes concert sponsorships at multiple area theaters and venues, the "Wild” drumline with the Milwaukee Bucks, guitar building workshops at Discovery World, and an original music stage at Milwaukee’s Summerfest. Cascio has been chosen as “Milwaukee's Best Music Store” by readers of “The Shepherd Express” newspaper for the past eight years in a row. Please visit

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Alzheimer’s Foundation of America to Offer More Family Respite Care Grants

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Program responds to rising national cost of dementia care

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As an unprecedented number of Americans face Alzheimer’s disease in their families, the Alzheimer’s Foundation of America (AFA) today announced the availability of the Phyllis and Milton Berg Family Respite Care Grants to nonprofit organizations for scholarships to cover the cost of respite care for family caregivers of loved ones with Alzheimer’s disease or a related illness.

The announcement comes on the heels of a new government-supported study that highlights the rising cost of caring for the nation’s dementia population, including informal, unpaid care, and direct costs for nursing homes, Medicare and out-of-pocket expenses that surpass that for people with heart disease and cancer.

The new AFA grants are named to honor the deceased parents of Barry E. Berg, of Commack, NY, who has served on AFA’s board of trustees for nearly a decade and has chaired several of AFA’s “Care on the Fairways” golf outings to support the organization’s programs and services. Both of Berg’s parents passed away in 2009: his mother, Phyllis, had Alzheimer’s disease, and his father, Milton, original owner of Bagel Hut and co-owner of the 19th hole of the Wynmoor Country Club in Coconut Creek, FL, devotedly cared for his wife during her nine-year illness.

“My siblings, Sheldon Berg and Bonnie Grossman, and I are extremely touched by this tribute and grateful that the grants will help caregivers in financial need who have selflessly dedicated themselves to taking care of their loved ones with Alzheimer’s disease,” said Berg, a CPA and co-managing partner of Nussbaum Yates Berg Klein & Wolpow, LLP, Melville, NY.

“Anyone who has walked in a caregiver’s shoes knows the devastation of Alzheimer’s disease on families,” Berg added. “It is unbearable and unacceptable to face this disease alone. These grants will provide financial relief as well as send a loud message to caregivers that it is ‘okay’ to take some time for themselves.”

AFA will award multiple grants of $5,000 each to its nonprofit member organizations nationwide via a bi-annual competitive application process. The grantees will use the funds to provide scholarships for respite care for family caregivers based on financial or other needs. These services include adult day programs, in-home aides or companions, or overnight stays at long-term facilities for individuals with dementia.

Applications are due June 1 and December 1 of each year. For more information, visit or call 866-232-8484.

“We appreciate Barry Berg’s long-time commitment to AFA and are honored to provide this beautiful legacy to his parents—one that recognizes the critical role of family caregivers,” said Bert E. Brodsky, AFA’s chairman. “By helping to lift some of the financial burden, these grants will also help relieve some of the emotional and physical impact of Alzheimer’s disease on our nation’s caregivers.”

The Phyllis and Milton Berg Family Respite Care Grants are becoming available at a pivotal point. According to the government-supported study released yesterday and led by a researcher at the RAND Corporation, in 2010, the cost of caring for people aged 70 or older with dementia in the United States totaled $159 billion to $215 billion, including $50 billion to $106 billion for informal care typically provided by family members at home. It estimated a tab of $41,000 to $56,000 per case, and projected that total national costs will more than double by 2040 to reflect the aging population.

The number of Americans with Alzheimer’s disease is expected to triple to 13.8 million by 2050, fueled in part by 10,000 baby boomers turning 65 each day. Advanced age is the greatest known risk factor.

An estimated 80 percent of people with dementia live at home, with family members serving as their 24/7 caregivers at the expense of their own physical and mental well-being. Studies show education and support services can delay nursing home placement of loved ones with dementia.

Evident of the financial burden on families, the average hourly rate for private-pay home health aides and companions is $21 and $20, respectively; and the average adult day program costs $70 daily, according to the 2012 results of the MetLife Mature Market Institute’s Market Survey of Long-Term Care Costs.

In addition to family respite care grants, AFA awards several grants to its nonprofit member organizations nationwide to develop or enhance programs or services in their communities; and holds annual college scholarship and video competitions for teens. To find out more about AFA’s awards, visit or call 866-232-8484.

The Alzheimer’s Foundation of America, based in New York, is a national non-profit organization that unites more than 1,600 member organizations nationwide with the goal of providing optimal care and services to individuals confronting dementia, and to their caregivers and families. Its services include a toll-free hot line, educational materials, a free quarterly magazine for caregivers and professional training. For more information about AFA, call toll-free 866-232-8484 or visit

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Adidas Unveils First Corporate Blog Maintained By Employees

Adidas Unveils First Corporate Blog Maintained By Employees

The sports apparel leader lets the world into its heart and company values as it showcases a wide variety of articles written by Adidas group staff.

Don Michael Acelar De Leon
  • 22 november 2011

In a remarkable attempt to create more intimate and unique relationships and foster online interaction with its customers, Adidas has recently launched its first corporate blog, written and maintained by the staff of the Adidas group.

Led by Jan Runau, chief corporate communication officer of the Adidas group, Adidas staff–composed of contributors from different departments–have written extensively on a wide variety of topics, such as brand identity, business operations, and football matches, among others, as far back as August of this year prior to the launch. The first official blog post discusses how the Adidas group’s approach on corporate blogging draws from the foundations of the company – sports, and the values that athletes live by.

Runau effectively summarizes the spirit of the people that form the heart of the blog:

The blog is authored and maintained first and foremost by adidas Group employees. We want to provide authentic insights and additional background about our company and the manifold business we drive in an industry which is one of the most inspiring and emotional of its kind. We want to conduct a genuine dialogue with everyone who is interested in what we are doing and want to explain how and why we are doing it.

Beyond the Group’s usual communication materials… a lot more is happening in our fascinating company each and every day – topics which are worth to share and which will hopefully  help you to understand the adidas Group even better – no matter if you are an applicant, a journalist, an investor, a sports fan who is interested in off-court issues like sports business or an adidas Group employee who seizes every chance to look beyond the nearby.

The launch of the blog ties in with Adidas’ recent efforts to bring itself closer to the people. The world can only eagerly anticipate the next social move of the sports icon.

Adidas Blog

Adidas Group

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