text:
(but it just don’t work on you) – Ian Phlegming

“My name is Bond,” he said to the blond in the red miniskirt sitting adjacent from him at the bar. “James Bond. What’s your name?”

The girl stared at James, nursing her cranberry vodka drink, mouth wide open in an expression that was equal parts shock and disgust.
“Would you like me to buy you a drink?” James asked with the brightest smile he could possibly manage.
The girl turned to her friend seated next to her at the bar, and the two quickly stood up and headed toward the restroom. James’ false smile quickly shifted to a frown. The bartender strolled up to him and asked him, “What’ll it be, mate?”
“I’ll have a pint of Harp,” said James disappointedly. He slumped into his chair and tried to ignore the gnawing sense of embarrassment in the pit of his stomach. He had spotted that blond girl the moment he walked into the Lion Head pub. She was in her mid-40’s, obvious divorcee, plain looking, but certainly not the most attractive lady in the bar. In his mind, though, she seemed the most within-his-league girl in the entire place.
James remembered the old days and cringed a little bit. He never used to settle on the easiest looking girl in the bar. In fact, the moment he stepped into a room, his aura of suaveness and sophistication had every girl automatically eating out of the palm of his hand, swooning over him passionately. In those days he hung out at fancier places – exclusive clubs, and casinos even, where he attracted women of more distinguished breeding, not to mention beauty. He never would have set foot in a place like the Lion Head, with its beer-stained bar tables, scent of stale cigarettes and body odor, not to mention the puddles of god-knows-what you had to skip over on the way to your torn-cushion seat. But these days, these were the only kind of places that would serve him, and the only kind of places where he could have a drink without feeling completely out of place.
The bartender slid a pint of Harp toward James and asked, “Shall I open a tab?” James nodded his head dejectedly. He took a deep long gulp. In the old days, he never would have stomached this kind of swill. It would’ve been all vodka martinis, shaken and not stirred. That little “shaken not stirred” twist was a big hit with the ladies back then. The problem was that as James got older it became more and more difficult for him to handle the ensuing hangovers that resulted from a night’s worth of martini binging. He’d be out of commission for the entire next day, nauseous with deep fatigue, and a splitting and unbearable headache. It got to the point where even a single drink would wreak havoc on his rapidly aging body, and the mere scent of hard liquor would send pangs of pukey hangover sensation through his head.
As a result, James started drinking more and more beer. It took more beer to give him a buzz than it did martinis, and soon he started to develop a magnificent gut. Within a couple months of the beverage shift his waist size went up 10 inches. Suddenly James looked terrible in a tuxedo, which had always been his trademark attire. It was around this time he started to develop other health issues, aches and pains in his joints, not to mention an on and off back condition that would often have him bed ridden for days at a time. He became slower, and had trouble staying up as late as he used to. Now he felt tired all of the time. None of this would’ve mattered much to the average person, except James was a secret agent for the British secret service, and up until around the time the Berlin Wall came down, one of the agency’s very best field operatives. He had saved the world from eminent disaster more times than anyone could remember, but now he sat slumped on a stool, with his gut jutting out towards the bar, sadly downing back his bitter pint of beer, his best days were clearly far behind him.
James slammed the empty glass onto the counter and watched for a minute as the foam slowly dripped to the bottom of the glass. He ordered a second pint, which he gulped down in even less time. He was starting to feel the fuzzy sensation of intoxication buzzing within his skull. By the time he was on his fifth pint, he felt he’d successfully gotten the better of his previous humiliation. He spun around in his stool and surveyed the bar. It was only a little past midnight, but the place was pretty quiet. It was only a Wednesday night.
There was a heavy-set and ratty haired old redhead wearing way too much makeup that failed to distract from her messed up teeth sitting at the other end of the bar loudly gulping down a tall glass of Guinness. He surveyed the room further, wondering to see what other women were in the bar. There were two or three twenty-something year olds seated at a table near the mildew caked dart-board – at least they looked twenty-something to James. He had long since lost the ability to correctly determine the age of anyone more than a decade younger than him. To him, everyone from the age of 17 to 39 looked relatively the same – young.
Young, and yet again, out of his league. The girls sat with a group of guys who looked to be their boyfriends. He thought for a minute about how with all of his advanced spy training it would be so easy to kill the boys. A quick snap of the neck, poison in their drinks, or a swift bullet from his Walther P38, fitted with a special silencer so no one would ever be the wiser. It’d be way too easy, but what good would it do? Three dead boyfriends, with girlfriends mourning over them, and James would probably still be going home empty handed.
He shrugged to himself, “The redhead will have to do.” He walked over to her end of the bar and sat down to order another round. “Hello,” he smiled at her. “My name is Bond. James Bond.” The redhead erupted in a hysterical giggle fit. “That’s an original line,” she said half sarcastically.
“I’m sorry,” said James, trying to maintain his smile and suppress the feeling of debasement he felt hitting on this woman who would have been so beneath him in his youth. “I just saw you sitting here all alone, just as I was, and thought I’d come over and chat you up.”
“People call me Gertrude,” she shot back. Gertrude Gravel.”
“What a charming name,” he said out loud with a hint of irony in his voice she could not detect. “Gertrude was also the name of Hamlet’s mother. A beautiful name.”
Back in those halcyon secret agent days, the women James was involved with had incredibly sexy and provocative names like “Honey Rider” and “Pussy Galore.” My how far he’d fallen, he thought to himself.
“Well, Gertrude. Would you like me to buy you a drink?”
“Ooohh-hoo-hoo,” she cackled in her thick cockney accent. “You can buy me a drink, luv, but don’t you be getting any funny ideas.”
“What’ll it be?” said the bartender who had descended upon the couple like a vulture.
“I’ll have another pint of Harp,” said James waving his near empty cup. “and the lady will have….” he motioned toward her expecting her to call out the brand of beer she was already drinking. Instead, she ordered a glass of Glenfiddich Scotch on the rocks, a very expensive brand. “Make it a double,” she added.
James recalled fondly a time when he didn’t need to buy women drinks to get them into bed with him. He didn’t have to spend money on anything and the women would still come swarming at him. He sighed heavily as the bartender poured the drink in front of them, Gertrude grinning at him now with her crooked teeth.
The two sat and chatted, and drank some more. With each passing drink, it seemed as though Gertrude’s teeth began to straighten out just a little bit. Nature was beginning to take its course, just as James had hoped and also sort of dreaded.
Finally the bartender yelled for last call, and James and Gertrude got up at the same time. Even through his alcohol induced numbness, James could feel shooting pains in his knees and lower back from having been seated for so long.
“Wait right here, luv,” said Gertrude as she quickly ducked into the ladies room. James felt like a mouse caught in a rattrap, debating whether or not he should gnaw his arm off and escape. He did a quick last minute scan of the bar to see if there were any better prospects than the girl he seemed to be going home with, but none presented themselves. He leaned up against the side of the bar, feeling exhausted, until Gertude returned from the ladies room and clasped his hand. The two headed out the door and hailed a cab.
The darkness of the cab accentuated the thick mascara surrounding her eyes. She looked like a raccoon, he thought to himself as he stuck his tongue down her throat. Her breath was repulsive, and her hastily applied make up smeared all over the place, but James didn’t seem to notice too much as they kissed and he rubbed her thick freckled arms. “Oh James,” she moaned in her gravelly cigarette damaged voice, as James’ hand moved underneath her dress.
The cab careened through traffic, blasting jittery Pakistani music as the couple continued making out in the back seat like school children. Finally it pulled up to a respectable one-person flat in London’s East End. James paid the cab driver and held his breath as he walked up the three flights of stairs to Gertrude’s apartment. All the way up she giggled hysterically between kisses, as she shushed at James, “We don’t want to wake up the neighbors,” all the while, her heels clomped loudly on the creaking wooden stairs.
The two made their way to the bedroom, where Gertrude turned on the lights and made her way to the adjacent bathroom, leaving a trail of removed clothes in her wake. While in the bathroom, James drunkenly inspected the light switch hoping to find some sort of dimmer function. When he couldn’t find it, he turned on the cheap Sony television set, and then turned the lights off. He took off all of his clothes and carefully slid underneath the blanket. His back and knees were still bothering him immensely, but he tried putting it out of his head for the time being.
Within minutes, Gertrude strut out of the bathroom clad in pink bathrobe. She came upon James fully nude underneath the blanket. “Why don’t you join me, darling,” he asked her with a wink in the darkness with glow of the television set tinting half his face as, while chat show host Jonathan Ross’ voice hummed quietly in background. She removed the pink robe and crawled under the blanket with him. The two continued to kiss, and Gertrude’s hand made it’s way below James’ waist. She clasped it in her fist and began stroking softly at first, then little by little increasing her speed and grasp.
The problem was, it seemed to be having no effect whatsoever; James and Gertrude were both starting to become self-conscious of this predicament. “Maybe we better slow down a bit,” James stuttered embarrassingly. He freed himself from her grasp, and moved below her neckline, where he began sensually kissing the wrinkled and fleshy mounds of flab. He slowly made his way below the belt and worked on that for as long as he could but the odor of a night’s long perspiration turned out too much for James to bear. He went back to kissing her again, and she grasped below his waist again, but once again to no avail.
“What’s the matter luv?” she asked. “Nothing,” said James. I think I just need a glass of water.” He fumbled out of the sheets groaning audibly and uncontrollably as he stood to his feet from the bed and headed toward the door “The kitchen’s second to your left. There’s clean glasses next to the sink,” said Gertrude. “Don’t take too long” she smiled. “Wild horses couldn’t drag me away,” winked James, as he took his cold footsteps toward the kitchen.
Once there he grabbed a clean glass and filled it with water from the faucet. He drank down the glass with one hand while his other worked furiously between his legs trying to desperately make himself ready, but he remained as limp as before. He drank three glasses of water, and sighed heavily. A stabbing sensation permeated his lower back sharply, as the effects of the alcohol had largely worn off. James felt fatigued, and his head throbbed with pain. He hovelled back to bed where he at this point hoped Gertrude had dozed off to sleep. Unfortunately she hadn’t. James smiled a convincing smile as he carefully underneath the sheets. He began kissing her again. At one point he tried to coax her head below his waist, but met with considerable resistance until Gertrude finally said, “I’m sorry. I just don’t really do that with someone I don’t know. Does that make me weird?” “No, not at all,” James lied. “You don’t have to do anything you don’t want to do.”
Gertrude moved her hand below James’ waist as the two kissed, and when she realized yet again she wasn’t going to get anywhere, she moved below her own waist and swiftly brought herself to climax.
“Sorry, luv,” she sighed exhaustedly. “But I just had to do it.” “It’s okay, said James,” sheepishly and unable to mask his humiliation. “I guess I must’ve had a few too many at the pub.” Gertrude ran her hands through his hair, patting him like a school boy, “Awwww, you’re so adorable. Let’s get some sleep. Maybe you’ll feel differently in the morning, and we’ll be able to give it another go.” She smiled and kissed him. Shortly thereafter she rolled over on her side of the bed and fell into a deep sleep. James cringed at the idea of waking up in this apartment with this woman and going through the whole ordeal again, but he was too exhausted to get up right away. He fought to keep his eyelids open, but soon drifted half to sleep.
In his sleep he had a strange dream. He stood before a great big concrete dam overlooking a high cliff. The rotting and decaying wall was all that stood between him and 9.2 trillion gallons of water. Suddenly, one of the cracks became more pronounced, and a leak spurted out in a thin stream of water. James put his finger over the crack to stop it, but he could feel the rumbling of raging waters from behind the wall. Soon the crack widened. James adjusted his hand to cover up the increasing damage, until pretty soon his entire palm was holding back water from the dam. Soon it began leaking and dripping from the palm of his hand, and soon another leak sprang from the upper left section of the wall. He reached up with his other hand to plug the leak, when suddenly he noticed another leak below that, but now his hands were full. He looked out the length of the wall, and saw it slowly beginning to crumble with cracks forming all along it, and new leaks sprouting out, until the wall started looking more like a shower-head.
The water just kept coming and coming, and he was drenched and powerless, soon the rumbling began to grow and large chunks of concrete began to fall from the wall until – woosh – a raging tide of water came cascading from an opening on the dam twice the size of his body. The waters swept him up, and he felt powerless as it dragged him down the side of the nearby cliff, presumably to his death.
He woke up startled with a nauseous feeling in his gut and an overpowering urge to pee. He looked at a nearby alarm clock. It read 4 a.m. Gertrude slept soundly, breathing with slight snores. James got up carefully and made his way toward the bathroom. After that, he put his clothes back on and decided to slip out of the apartment unnoticed. He didn’t want to hail a cab, hoping to use the brisk morning walk to clear his head and reflect a little on the previous night’s series of disappointing events, but his legs quickly became sore, and nausea overcame him from the night’s drinking. He realized he had no choice but to hail a cab.
James reached a busy boulevard and in ten minutes had hailed down a cab. He fought hard to stay awake on the cab ride home, and couldn’t manage the strength to protest when he realized the driver was taking him the long route home. “I’m getting to old for this shit,” he thought to himself.
The cab eventually pulled up to his King’s Row apartment in Chelsea, and the morning sun began to rise. The chirping birds and sounds of children playing in a nearby lot irritated James as he dragged himself to the elevator and up to his apartment. He passed by the lobby security guard on his way to the elevator. “Long night, Mr. Bond?” James nodded exhaustedly, with deep bags underneath his eyes that seemed to perfectly garnish the quivering frown beneath them. In his apartment he stood before the mirror in his living room and carefully examined these facial features, which only made him more miserable. He studied the wrinkles that seemed to line his face like routes on some sort of complicated and cluttered road map. His hair color had changed from a sleek black color to a coarse and scraggily gray.
He looked around the room and everything was out of place and unkempt. In his bedroom the floor was covered with dirty laundry. James had lived extravagantly in his youth, and neglected to save much. He was trying to get on top of the crippling debt that plagued him now, and certain luxuries, such as a maid had to go. His salary as a field operative for the British secret service had not changed much in 20 years.
James had not slept in his apartment in over a month, and felt glad to be back as he lay down underneath his bed sheets. He had been to Afghanistan on assignment. These days the work of a secret agent was not as exciting as it once was. It mostly included hiding out in remote desert outposts, intercepting and cataloguing stray radio transmissions from nearby terrorist cells. It was tedious work. James pined for the days before the fall of communism when field operations meant hanging out in casinos, drinking martinis, and shacking up with exotic Eastern European women.
James could feel an immense soreness in his back as he went into the freezer and pulled out and ice pack he kept specially prepared for just such occasions. He went into the cupboard and pulled out a prescription vial of oxycodone, popped 4 into his mouth and swallowed it with a glass of tap water. He lay on the couch in the living room with the ice pack placed on his lower back, while Vivaldi gently emanated from his stereo system. He fell into a deep and satisfying sleep that lasted a total of 13 hours. When he woke up, he still felt quite exhausted, but trudged out of bed anyway.

When you’re young, not much matters. When you find something that you care about, then that’s all you got. When you go to sleep at night you dream of pussy. When you wake up it’s the same thing. It’s there in your face. You can’t escape it. Sometimes when you’re young the only place to go is inside. That’s just it – fucking is what I love. Take that away from me and I really got nothing.


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Michael Figuccio

Assistant Professor, Psychology · Farmingdale State College

Dr. Figuccio's teaching interests include child development, atypical development, physiological psychology, and introductory psychology.

Autism Spectrum Disorder Developmental Dyslexia Child Development

Eileen Kennedy-Moore

parenting & child development expert - author, psychologist, speaker

Parenting and Child Development Expert with National Media Experience

Psychology Parenting Child Development Social & Emotional Learning Mental Health

Claire Vallotton

Associate Professor of Child Development · Michigan State University

An expert in early childhood education and intervention; parent-child interaction and communication; early development of social skills

Childcare Infant Mental Health Parenting Early Childhood Education Child Development

Sheri Kelfer

Adjunct Professor · USC Suzanne Dworak-Peck School of Social Work

Sheri Kefler is a social worker, psychotherapist and child development specialist, focused on sexual, physical and emotional abuse.

Trauma Including Childhood Sexual Abuse Child Development Physical Abuse Sexual Abuse Emotional Abuse

Mariana Brussoni

Associate Professor · University of British Columbia

Brussoni's research focuses on child injury prevention, including risky play, parents' perspectives on safety, and Aboriginal injuries.

Injury Prevention Child Development Parenting Outdoor Play Higher Education

Tiffany Field, PhD

Doctoral Faculty - Clinical Psychology · Fielding Graduate University

Over thirty years of research on the positive effects of touch

Infant Mental Health Alternative Therapies High-Risk Infants Depression Research Methods

Juan Araque

Clinical Associate Professor of Social Work · USC Suzanne Dworak-Peck School of Social Work

Dr. Araque is an expert in leadership, education, community development, diversity, violence prevention and positive youth development.

Leadership, management and social policy in the public and not-for-profit sectors Amber Alert Adolescent Development Violence Prevention Youth Development

Carla Cesaroni, PhD

Associate Professor and Assistant Dean, Graduate Studies, Faculty of Social Science and Humanities · University of Ontario Institute of Technology

Leading international authority on youth justice, and highly sought expert witness

Corrections/Penology Youth in Custody Youth Justice Offender Reintegration Male Young Offenders

Kate Einarson, Ph.D.

Knowledge Translation (KT) Specialist · Holland Bloorview Kids Rehabilitation Hospital

I am a behavioural scientist with a passion for getting research evidence out of scientific silos and making it useful to people who need it

Science Communication Language and Literacy Mental Health and Substance Use Arts Education Music and Mind

Amy Nuttall

Assistant Professor of Human Development and Family Studies · Michigan State University

Amy K. Nuttall is an expert in the areas of family relationships, parenting, and how families cope with stress.

Family Dynamics Maltreatment Parenting Caregiving Quantitative Psychology

Tanya Kaefer

Assistant Professor, Faculty of Education · Lakehead University

Researcher in educational psychology with expertise in learning, knowledge, reading, and cognitive development.

Reading Development Cognitive Development Early Learning Learning & Development Media and Learning

Erik Schott

Clinical Associate Professor of Social Work · USC Suzanne Dworak-Peck School of Social Work

Dr. Schott is a licensed clinical social worker and instructional designer/educational psychologist.

LGBTQ (lesbian, gay, bisexual, transgender, queer) social work CBT (Coginitive Bahavorial Therapy) EMDR (Eye Movement Desentization & Reprocessing) HIV & AIDS Childhood Developmental Dirorders

Ira Glovinsky, PhD

Adjunct Faculty - Infant & Early Childhood Development - School of Psychology · Fielding Graduate University

Early childhood development and mental health expert

Clinical Psychology Special Education Infant and Early Childhood Development Research in Child Development Mood Disorders in Infants, Toddlers, and Preschool Aged Children

David Cross

Rees-Jones Director and Professor, Psychology · Texas Christian University

David Cross develops programs like TBRI to improve the lives of children who have experienced abuse, neglect, and/or trauma.

Research, Education and Outreach to Improve the Lives of Children who have Experienced Abuse, Neglect, and/or Trauma Trust-Based Relational Intervention® (TBRI®)

Jenene W. Craig, PhD, MBA, OTR/L, CNT

Program Director - Infant & Early Childhood Development - School of Psychology · Fielding Graduate University

Neonatal health; Collaborative, progressive leadership & management with clinical experience in non-profit higher education

Neonatal Intensive Care Infant and Early Childhood Mental Health Trauma-Informed Care Infant and Early Childhood Development Parent-Infant Attachment

Jenny Edwards, PhD

Doctoral Faculty - School of Leadership Studies · Fielding Graduate University

Invitational Education, Cognitive Coaching, Nonverbal Communication

Action-Orientated Research Capacity Building Child Development Community Relations Creativity and Problem Solving

Shayne Piasta

Associate Professor | Teaching and Learning · The Ohio State University

Expert in reading and literacy in early and middle childhood education.

Child Development Early Childhood Education Childhood Literacy Literacy Reading and Literacy

Dr. Eileen Kennedy-Moore

Author, Psychologist, Parenting Expert

nationally recognized authority on children's social and emotional development ---- "inspiring audiences with wisdom, compassion, & humor"

Parents Parenting Children Child Development Children's Friendships

Michelle Grimes

Assistant Professor of Child Clinical Psychology · Southern Utah University

A licensed psychologist specializing in clinical child psychology, principles of phycological assessment, and sibling conflict resolution

Caffeine and Depression Sibling Conflict Resolution Teaching Parents to Code Child Behavior Sibling Conflict School Concerns

Joseph Bush, PhD

Faculty Research Specialist - Clinical Psychology · Fielding Graduate University

Pediatric Psychology; Child Pain; Parent-Child Interactions in Stressful Medical Situations; Psychological Effects of School Gardens

Health Psychology Pediatric Psychology Statistics Child Development Pain Assessment and Management

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text:
publication . Article . 2015

Potentials of Local Economic Development in Aspect of Tourism

Viktória Csizmadiáné Czuppon; Edina Sáriné Csajka; Tamás Molnár;
Open Access Czech
  • Published: 01 Oct 2015 Journal: DETUROPE (issn: 1821-2506, Copyright policy)
  • Publisher: Društvo za Regionalne Nauke
The objective of the study is to introduce the potentials of local economic development in one of the least favoured micro regions, Tamási. The paper examines operating and planned activities at settlements of the micro region. The authors introduce local economic development activities that support tourism. The economic development planning in Tamási micro region has typically two directions. One of them is the utilisation of thermal water and the use of further potentials of the thermal bath (Ability), such as to achieve tourism destination function in Hungary. Secondly, the local government aims to sell its fruits and vegetables produced in the frame of publi...
free text keywords: local economic development, disadvantages micro region, micro region, Recreation. Leisure, GV1-1860, Urban groups. The city. Urban sociology, HT101-395
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text:
Meet Quark Expeditions Polar Travel Adviser Naomi Box

January 7, 2015

Planning and prepping for a polar expedition can be exciting and challenging – it's a GREAT BIG DEAL!

We get it. That's why Quark Expeditions offers the services of our highly knowledgeable and experienced Polar Travel Advisers (PTAs). Whether you're just looking for information, or have already booked and are planning for your upcoming polar adventure, our PTAs are packed with resources, tips and guidance to help you make the absolute most out of your arctic or Antarctic holiday.

King penguins

So just who is it on the other end of the line when you call a Quark PTA? In this series, we'll introduce you to the wonderfully knowledgeable people who help passengers with every aspect of their expedition preparation and planning.

Meet Naomi Box, Quark Expeditions PTA

Naomi on a landing in Antarctica

Naomi works out of the Quark Expeditions office in Toronto, Ontario. Her enthusiasm for polar travel is limitless, and she puts this passion into practice in her consultations with Quark passengers.

Early in life, Naomi spent a month attending school in Australia, where she caught the travel bug. For many years since then, she's worked in the travel industry in Canada and the U.K.  A traveler to over 20 countries, Naomi first visited Antarctica in February 2012. More recently, she traveled to the Arctic this past June. One of her goals at work is to help each traveler discover how to bring their own economic, environmental and social benefits to any destination.

Where did you go in Antarctica?

"I went on the Antarctic Explorer expedition voyage in 2012. A lot of people don't realize just how diverse Antarctica is, but there are over 200 Antarctic landing sites there! Every trip literally is different, as the weather guides which landing sites we'll visit. Quark's on-board staff monitor the conditions constantly and safety is always first. On my trip, we visited Port Lockroy, Deception Island, Aitcho Island, and Wilhelmina Bay."

Naomi and Quark passengers in Antarctica

How do you learn about the areas travelers have questions about?

"I've been to all 7 continents and spent a lot of time traveling in my youth. Lonely Planet was a great resource for my own travel and I also watched documentaries and reviewed tourist board websites, if they were available.”

My greatest resource now is our staff. A lot of people in the office are really educated and regularly travel to Quark's polar travel regions. We each travel with Quark and teach one another about the areas we visit."

What's your favourite place you've ever traveled to?

"Once I visited Antarctica, it became my instant favorite. We think of penguins, ice and harsh conditions, but I've never seen as blissful a place as the Falkland Islands. We stepped on this white sandy beach with ducks, sheep, seals, dolphins, and penguins… it's really surprising and unexpected. It's made me want to go back.

Getting ready to travel to the Polar Regions, or even just considering booking a polar holiday, can cause people to feel anxious, excited, elated – a lot of different things! For a lot of people, it's the trip of a lifetime and they want to be prepared. So we hear a lot of common questions.”

Excited to be in Antarctica with Quark Expeditions!

Common Arctic or Antarctica Questions

Do we help with flights and insurance? “Yes.”

What should I pack? “We provide packing checklists to help people prepare.”

Am I going to see wildlife? “This is probably the question I'm most frequently asked. We can't make guarantees, but we go to the best places we possibly can at the time and our expedition teams are tuned in on that.”

A rockhopper penguin

How often might you speak with a traveler before their trip?

“Often. These expeditions are a type of “calling” for people. They might not go right away, but they will go, even if it's a few years from now. People who go to Antarctica are adventurers. Going to the Arctic is an exploration – you're in search of things.

The first phone call usually lasts about 20 minutes. People tend to have done their research by the time they decide to take the next step and talk to a PTA. There's a lot of excitement, but also a lot of apprehension.

We (PTAs) usually talk to travelers several times, as they decide on their trip, plan and prepare, and then even afterwards. I love getting phone calls from passengers telling me about all the amazing things they did and saw!"

What resources does Quark provide in advance of a trip?

"Each passenger receives a booklet about their ship so they can prepare for life onboard. A detailed itinerary lists the activities, adventures and landings they might see and participate in. We have two fantastic publications, the Arctic Reader and the Antarctic Reader, packed with information – each passenger will receive the publication for their region.

Of course, passengers can always access the PTAs! We're happy to talk anytime about any questions or concerns passengers have."

Is sea-sickness a concern? “If this is a concern for you, there are excellent options nowadays to prevent this, even for passengers who may suffer from this it quite severely.”

What do you love most about being a PTA?

"There's so much to see in the Polar Regions but we also have to be really mindful of how we conduct ourselves in these places. I love this job because this type of travel changes people. The only way we're going to keep Antarctica pristine is by turning people into ambassadors and I'm so proud to be a part of this process. It's great when I hear my clients come back and they're so excited and changed by the travel experience. Each PTA has clients who call us six months after their trip to talk. You just feel very grateful for being able to travel to these regions!"

To speak with a Quark PTA, call 1-844-249-2056 or outside of North America, call 1-802-490-7533.

Previous Article
Letters From Antarctica-Diary from a Polar Expedition-part 4
Letters From Antarctica-Diary from a Polar Expedition-part 4

The conclusion to our "Letters from Antarctica" series. Quark expedition team member Simon Bottomley shares...

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Letters From Antarctica-Diary from a Polar Expedition-part 3
Letters From Antarctica-Diary from a Polar Expedition-part 3

Our "Letters from Antarctica" series continues with part 3! Read to discover what day-to-day adventures you...


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Question: Can IMEI Number Be Erased?

Can your IMEI number be traced?

Using the IMEI number to track the lost phone is not the only function of an IMEI tracker.

Thus, you can control the stolen phone by sending the corresponding codes to make it give out alarms, send SMS about its real-time location, sync data to the device you are using and more..

Can a phone be tracked when switched off?

A phone which is switched off will not be able to emit any signals and will stop communicating with nearby cell phone towers. You can only trace it through its last location when it was switched on by calling your service provider or through Google services.

How can I block my IMEI number online?

The IMEI code: to block a lost or stolen handset This is a 15 or 17-digit number that is unique to each phone. You will need to give this number to your telecom provider so they can block your SIM card if your handset is lost or stolen.

What happens when you block IMEI number?

If a phone is blacklisted, it means that the device was reported lost or stolen. The blacklist is a database of all the IMEI or ESN numbers that have been reported. If you have a device with a blacklisted number, your carrier may block services. In the worst-case scenario, the local authorities could seize your phone.

Can I block my phone without IMEI number?

In order to block your phone without your IMEI number you need to contact customer care for whichever provider you are with, and they can do one of two things; 1 they can block your sim, this prevents calls coming or going from your sim card or 2 they can at your request look up your IMEI number, they have this with …

Can a lost or stolen phone be activated?

A stolen phone can not typically be activated. … Some country’s carriers won’t activate a stolen phone from another country because they are partners of sorts and share a blacklist. Some carriers will activate it because they don’t share the same blacklist.

Can a blacklisted IMEI be unlocked?

Identify Hybrid CDMA/GSM Phones. If the phone is unable to activate on a CDMA carrier such as Sprint or Verizon, the blacklisted IMEI could still have the ability to be used on a GSM network. … Likewise, many of them come factory unlocked, even if one of the major providers sold the phone.

What happens if IMEI is changed?

Changing the numbers could damage your device in the process. Legally, you have forfeited your ownership of your mobile device. When you purchase your device, the seller will jot down the original IMEI number on your receipt. So if you change your IMEI and lost it, you may not be able to make a claim on it.

Should you give your IMEI number out?

It’s commonly recommended as a way to avoid buying a stolen phone. … Some sellers have lost their phones’ usability to hackers after giving the IMEI out to criminals masquerading as potential bidders. The safest bet is to only give the IMEI number to someone who has already paid for the phone.

Can IMEI number be blocked?

Blocking the IMEI number of a stolen device won’t help, if the number itself gets changed after theft. So, in a scenario like this, IMEI blocking is of no help in protecting lost or stolen mobile devices from being misused or resold as second hand equipment.

Can I track my stolen phone using IMEI number?

Once you get to know the IMEI number of your stolen phone, it is easy to track it for free using an IMEI phone tracker app that you can download from the Google Play store. Apart from this, you can contact the police and provide them with your phone’s IMEI number so that they can track your lost phone.

Can IMEI number be changed?

No you can’t change IMEI number of your phone. The IMEI number is set during the installation of os by the manufacturer.

Can thieves change IMEI number?

The government has made tampering of IMEI numbers a punishable offence. However, thieves change the IMEI number of stolen mobiles using a ‘flasher’. A flasher is a small device that helps connect the handset to a computer and allows the user to modify the IMEI number.

Can a stolen phone be unlocked?

Android. You can remotely lock your Android device via the Find My Device app (which needs to have been turned on before the device was stolen). Use Gmail credentials to sign in to from a web browser and select SECURE DEVICE. You’ll then be prompted to set a new lock screen password.


text:
MINIX Z83-4 Fanless Mini PC Desktop Windows 10 OS (64-bit) Intel Cherry Trail Quad-Core X5-Z8300 64-bit 4GB/32GB Gigabit Ethernet 2.4/5G Wi-Fi Dual Output Mini DP and HDMI 1.4 BT 4.2 Streaming Media Player

  • Minix Z83-4 adopts newest and latest Intel Cherry Trail Quad-Core(Intel X5-Z8300 64-bit) based on Fanless Mini PC running licensed Windows 10 OS (64-bit), delivering unrivalled combination of ultra-compact design and premium performance that is ideal for work, play and entertainment.
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  • Equipped with dual output display ports (Mini DP and HDMI) and combining awesomely fast start-up, seamless web browsing, mufti-tasking and compatibility with a vast array of office productivity software.
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text:
extension ExtPose

HTTP Request Blocker

Description from extension meta

This extension blocks HTTP Request based on configurable URL patterns.

Image from store HTTP Request Blocker
Description from store Blocks HTTP requests based on URL patterns. The patterns must conform to this specification: https://developer.chrome.com/extensions/match_patterns. Source code is available at: https://github.com/wolfascu/ChromeHttpRequestBlocker

Latest reviews

  • (2020-01-20) Murphy Chu: Awesome! very very helpful when some website have many useless request Thanks~
  • (2019-04-05) Eru Magus: 2019/04/05 No funciona
  • (2017-09-15) Fabrice Denis: Unfortunately it doesn't match when using the "omnibar" autocomplete, (ie. type a few letters in address abr and then enter to pick a site you've visited before). For example if I block reddit.com/r/videos I start typing in address bar "reddit" and the videos sub will appear as a suggestion from history. Then I press enter and it isn't blocked, because the pattern matching seems to take place before the "omnibar" search is replaced with the complete url appearing in the dropdown.
  • (2017-06-29) Walid Aqleh: just what I needed to test payment gateway if the thankyou page was not accessible.
  • (2017-06-28) G L: Hello, thanks for your work but this extension does not block ALL http/https requests according to the users' correct expression given/saved. It only blocks requests from the addr bar/field. If a page is making http requests for example to display an image, those requests are NOT blocked. Example: I tried to block the youtube logo, it gets blocked if I enter the logo's full url in the addr bar, but the logo shows up on the youtube home page (after doing everything CORRECTLY, i.e. clearing the browser cache, restarting, etc.)
  • (2016-09-01) gal773: It works but you need to be very careful with what you enter. The program does not have any feedback on what you enter. If your URL expression is not correct it just stops blocking all URLs. When it works - it works!
  • (2016-01-28) Nuno Faria: Worked on 1st try. Good!
  • (2015-10-28) Davel Liu: Love this. If only it can block request based on domain.
  • (2015-07-28) Carlos Sanchez: It blocks page requests and doesn't have an intrusive interface. Make sure you read the example page linked in the description.
  • (2014-12-14) Awesome! Exactly what I needed!

9,706 history
4.2 (17 votes)
Last update / version
2020-08-17 / 1.0.6
Listing languages


text:
Forbidden fruit*

In Florida I saw this pair of Red-bellied Woodpeckers (Melanerpes carolinus) on the top of a dead palm tree.

The Red-bellied Woodpecker (Melanerpes carolinus

We all know that woodpeckers probe rotting wood to retrieve insects, ants, and juicy larvae, so dead and dying trees are where you expect to see them.

But this one had other ideas:

As you can see, they are in fact quite catholic in their tastes, and often eat seeds and fruits, not just when kindly supplied for them by human bird-feeders.  This one is a male, because the red covers his whole head; females have red napes only. And you can just glimpse the red on his belly that gets him his name.

All over the world, fruiting trees are irresistible to wildlife. This mousebird in Ethiopia is gorging on figs:

Speckled Mousebird

And this bulbul can barely swallow the large yellow berries he has found:

Common Bulbul?

The size of his belly suggests that this Kenyan Blue Monkey has already had more than enough:

Blue monkey, Cercopithecus mitis

* These greedy, wary, faintly illicit feastings reminded me of Emily Dickinson’s poem:

Forbidden fruit a flavor has

That lawful orchards mocks;

How luscious lies the pea within

The pod that Duty locks!

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text:
2019-02-13 Ivey Backblast

Tarde, Shear Conn (Soul mate), Crawdaddy, Serta, Tramp Stamp, Kryptonite, Crankbait, Gilmore, Fud, Duvet, Chipper, Dean (QIC)

12 PAX including the Q came out to see if we could hit 6,000 steps…And just to note as the Fake news (Bezos and Amazon Propaganda spewing) Washington post says, Democracy dies in darkness. So does the voting….Take that Bambi and Tarde…Despot Dean took the reigns…

And in the Gloom….
Despot Dean is Back

We started with a mosey to the end of the road for Warm-a-rama of:

Dean Vigodas (yes I’m vain)

Seam Rippers

Back Stretch

Then run between each light 1x SSH at first, 2x SSH at second, etc. All the way to shields road. Then back down but Squats increasing.

Then a mosey to the big parking lot for suicides with 3 merkins at each turn.

Then the longest Dips, Derkins, Step-ups. Go between concession stands, 2x.

Then run super long route to Mary’s to get to 5,800 steps or so. After Mary’s we did High knees until hitting 6,000 steps.

Random Musings:

  1. Shear Conn and Kryptonite had to do Burpees despite Dean’s promise of no burpees. That’s what you get for coming in first folks…EXTRA credit. Shear Conn accused Dean of growing up with Clinton as president…All I can say about that is the Clintons are Ross Perot’s fault, and I’ll never forgive old Rossy for that.

2. Tramp Stamp was giddy as a school girl after Duke came back from winning last night.

3. Anyone else notice how Serta always says Name-a-rama different? As a control freak and despot, I almost commanded burpees, until I said my own age wrong and had to do them myself…

4. Fud sounds like Darth Vader when breathing heavily. Absolutely fantastic! Wish I had that power. Good work Fud!

5. We hit 6000! Amazing achievement! Keeping with the Clinton theme…

6. Kryptonite was trashed as being an FNG…not cool…I think he just loves a good Dean Q….

Announcements/Prayer Requests

-Pray for those doing pilgrimage this weekend

-Pray for Bambi to get better! His back is sore from previous rugby injury per TARDE

-Also Thin Mint got into TEXAS A&M. Go Thin Mint…I mean it is the SEC, so substandard education, but fantastic ROTC. Great work Thin Mint!


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Lord, Give me a Clean Heart

His lightning strikes Like spikes Through the hearts Of all who need a fresh start Some may know and seek Others hide behind what they speak He shakes the nations Nobody wakes He destroys houses Nobody bows down What will it take For those who claim To follow Jesus To admit their diseases I will … Continue reading Lord, Give me a Clean Heart

a grief story

when your mom's gone that grief that overcomes and powers through your only mom she had a way of seeing you   seeing you in your darkest days swimming through seas of shame she knew secrets you didn't know your mama, that one of long ago   the mama who cradled you now gathers round … Continue reading a grief story

Such misery In the mystery Gunmen shoot cops down Satan tears the Bride's gown Children missing but alive Forced to do things, to sexualize Inside His church Hands grope, evil searches Jesus is under no veil Victory is His sail Eternity is not available For those who run with evil His banner is lifted high … Continue reading Harmony

Crystals hardening A new day dawning His blue hues His misty morning Canada air Frozen here Covering the window Of my sleepy hollow Where fear and lies deeply burrow Frothing, freezing Heavy feeling What once was clear Now encrusted … is He still near? Why can’t I see The eyes who made me? When will … Continue reading Unfrozen

Jesus is the Way

Pursuing Unending Never bending To our words Heeding and bleeding For the Higher Will Of Our Creator Jesus dying Not saving Himself But choosing us Every last one Nobody is done Until He mounts The clouds Riding in Glory To a new Beginning Death rising Doubters realizing Our Lord is true Not a myth When … Continue reading Jesus is the Way


text:
know the effects of OSM on trophoblast migration, proliferation,

know the effects of OSM on trophoblast migration, proliferation, and the invasion of EVTs in hypo ic environments. Recently, it was reported that recombinant interleukin 6 click here and TNF were capable of activating endothelial cells, which is a hallmark of preeclampsia. Another study dem onstrated that IL 6 stimulates cell migration and inva sion accompanied by the increased e pression of related integrin subunits on the HTR8 SVneo cell line, al though the former study only suggested the effects of IL 6 on EVT invasion cellular cascades. LIF, a mem ber of the IL 6 family, has been suggested to increase the invasiveness of trophoblastic cells through the acti vation of STAT1 or STAT3. Because OSM is a cytokine in the IL 6 family, its role in activating endo thelial cells should be investigated to evaluate the role of OSM in the preeclamptic placenta.

The func tional role of OSM in the human placenta has not yet been clarified. Because OSM Inhibitors,Modulators,Libraries has cell type specific ef fects, the effects and mechanisms of OSM related to normal and pathologic pregnancies should be evaluated both in vitro and in vivo. Conclusions Taken together, these data suggest a contributing role for OSM in stimulating the migration of EVTs during the first trimester through down regulation of E cadherin. The effects of OSM on E cadherin and the migration of the trophoblasts were related to STAT3 Inhibitors,Modulators,Libraries activation, which is important for trophoblast invasiveness. Further re search is needed to investigate the various roles of OSM in normal and pathologic pregnancies under hypo Inhibitors,Modulators,Libraries ic conditions, including how this cytokine interacts with other regulating molecules.

Inhibitors,Modulators,Libraries Background Mesangial cells response to various pathological stimuli associated with the main events of glomerular in flammation, including leukocyte infiltration, cell prolifera tion, and fibrosis, which were predominantly mediated through induction of adhesion molecules. In bacteria induced glomerulonephritis, lipopolysaccharide AV-951 stimulated VCAM 1 induction in the murine glomerular mesangium. It has been also reported that Toll like receptor 4 activation by LPS increased the e pression of adhesion molecules, such as VCAM 1 which recruits leucocytes to the kidney. Reactive o ygen species are known to play a prominent role in the pathogenesis of various renal disor ders, such as nephropathy, renal ischemia, and renal fibrosis.

Nicotinamide adenine dinucleotide phosphate o idase is an important enzymatic source for the production of ROS under various patho logic conditions. NADPH o idase derived selleck chemicals ROS have been shown to induce monocyte chemoattractant protein 1 e pression in MCs leading to nephropathy. Acti vated NADPH o idase is a multimeric protein comple , including p47pho cytosolic subunits. It has been shown that the phosphorylation of p47pho results in its mem brane translocation and activation of NADPH o idase. It has been reported that ROS generation is neces sary for VCAM 1 induction in IL 1B treated human tra cheal smooth muscle cells.

d proteins and HIV 1 enzymes, 10 ug of plasmid coding for the VSV

d proteins and HIV 1 enzymes, 10 ug of plasmid coding for the VSV G envelope, and 40 ug of len tiviral vector with GFP driven by CMV. The medium was changed 10 h after cotransfection. Supernatant was selleck chemical har vested 72 h after contransfection and centrifuged 15 min utes at 2500 rpm. Supernatant was filtered through a 0. 22 um filter and then ultracentrifuged 90 minutes at 50,000 g. The pellet was finally resuspended in 100 ul PBS. Transduction Macrophages, isolated as previously described in 24 well plates, were Inhibitors,Modulators,Libraries preincubated with or without rottlerin dur ing 2 hours and then incubated 3 h at 37 C in 250 ul of Iscove medium 2% FCS containing 50 ul of VSV G GFP vector per well, in presence or absence of inhibitors. After 2 washes with PBS, cells were cultivated in Iscove medium, 10% FCS.

Inhibitors,Modulators,Libraries After 2 days, cells were visualized with a fluorescence microscope. Q PCR 5 105 macrophages well in a 24 well plate were incubated for 3 h at 37 C in the presence of HIV 1 BaL virus pretreated with DNase I. Cells were Inhibitors,Modulators,Libraries then washed with HBSS and Iscove medium, 10% FCS, 1% penicillin streptomycin was added. Cells were then washed with HBSS at different times after infection. DNA was then e tracted. For the detection of early and late reverse transcripts, DNA was amplified with the appropriate primers at 70 C in a LightCycler with SYBR Green following the manufacturers recommenda tions. Viral DNA was normalized by cellular genomic GAPDH. Actin cytoskeleton analysis Macrophages were resuspended and placed in wells containing a glass slide. After two cycles of adherence, macrophages were Inhibitors,Modulators,Libraries washed 2 times with PBS, and then fi ed with PBS medium 3.

7% formalde hyde for 10 minutes at room temperature. After two AV-951 more washes with PBS, macrophages were permeabilised by a 5 min incubation in the presence of 0. 1% TRITON 100. Two washes with PBS were performed, then cells were blocked with PBS 1% BSA for 30 min to avoid non specific labeling. Cells were then labeled with phal loidine rhodamine for 20 minutes at room temperature. Macrophages were washed two more times with PBS and then mounted on cover slide using moviol and placed at 4 C until observation. Macrophages labeled with phalloidine rhodamine were observed under a con focal microscope equipped with a 568 nm laser to e cite the probe. 50 cells per slide were counted on at least 2 different slides per condition.

Cells with clear pseudo podes were counted Calcitriol IL-2 as positive while cells without pseu dopodes or with small rare pseudopodes were negative. All results were normalized to control cells. Syncytia formation HeLa R5 4 were cocultured with HeLa gp120 gp41LAI or HeLa gp 120 gp41ADA in 96 well plates in the presence of various concentra tions of each inhibitor. After 20 h, syncytia were scored by contrast phase microscopy. Background The human astrovirus, a member of the Astroviridae family, is a small non enveloped virus with a 6. 8 kb, positive sense RNA genome bound at the 5 end with the viral protein Vpg and polyadenylated at

previously reported a comprehensive study of AMPK subunits in ova

previously reported a comprehensive study of AMPK subunits in ovarian customer reviews cancer and showed that all subunits are generally upregulated in ovarian cancer. Intri guingly, the overe pressed AMPK B1 that was found in early stages of ovarian cancer were significantly reduced in advanced stage ovarian cancer. Given that post translation modifications of AMPK B1 are essential for AMPK activity, the e pression status of AMPK B1 may determine the AMPK activity in ovarian cancer progression. In this study, we further investigated the e pression and functional roles of the AMPK B1 subunit in ovarian cancer. We demonstrated a progressive reduction in the e pression of the AMPK B1 subunit from early to late stage ovarian cancer, whereas enforced e pression of AMPK B1 could inhibit the cell growth and other aggressive capacities of ovarian cancer cells through the AKT ERK and JNK sig naling pathways.

Overall, our findings underscore the im portance of AMPK B1 in carcinogenesis through its ability to modulate AMPK activity and other oncogenic pathways during the progression Inhibitors,Modulators,Libraries of ovarian cancer. Materials and methods Ovarian cancer tissue array and cancer Inhibitors,Modulators,Libraries cell lines Four ovarian cancer cell lines were used A2780cp and OV2008 were obtained from Prof. B. K. Tsang, and SKOV3 and OVCA433 were obtained from ATCC. Cell line authentication was per formed using an in house STR DNA profiling analysis, and the cell lines were cultured in minimum essential medium supplemented with 10% FBS inside an incubator containing 5% CO2 at 37 C.

An ovarian cancer tissue array, which consists of five cases of normal benign tumors and 97 cases of ovarian cancers, was used for immunohisto chemical analysis. Plasmids and DNA transfection The pcDNA3. 1 AMPK B1 Flag tagged plasmid was used to overe press AMPK B1 in ovarian cancer cells, and Li pofectamine 2000 Transfection Reagent Inhibitors,Modulators,Libraries was used for transfection e periments. Stable AMPK B1 over e pressing clones were established from AMPK B1 trans fected cells using G418 selection. The shRNA plasmid shRNA AMPK B1 for targeting against AMPK B1 was purchased Inhibitors,Modulators,Libraries from OriGene Technologies, Inc. Stable, AMPK B1 knockdown clones were established by puromycin selection of shB1 transfected cells, and all of the clones were verified by western blot analysis. The pEGFP AMPK Carfilzomib B1 plasmid was used for im munofluorescence analysis and was constructed by sub cloning AMPK B1 from the pcDNA3.

1 AMPK B1 Flag tagged sellectchem plasmid into pEGFP C1. Western blot, immunofluorescence and immunohistochemical analyses Cells were lysed in lysis buffer containing a protease inhibi tor and phenyl methyl sulfonyl fluoride. Equal amounts of each sample were fractionated by SDS PAGE and electroblotted onto an Immobilon P Membrane. The membrane was blocked with 5% non fat dry milk in a TBS with Tween solution at room temperature for 1 h, followed by overnight incubation with various primary antibodies. Antibodies against AMPK B1, AMPK, phospho AMPK, P70S6K, phospho P70S6K, AKT, phospho AKT, mT

etalloproteinases This indicates a functional relationship betwe

etalloproteinases. This indicates a functional relationship between Kallik reins and the vascularisation related gene, placental growth factor Pgf and the Thrombospondin 2 gene Thbs2 which showed close selleck kinase inhibitor correlation with these genes. Given the Inhibitors,Modulators,Libraries prominent angiogenic phenotype that develops in these pre cancerous skin lesions follow ing MYC activation, it is reasonable to speculate here that Kallikreins and Pgf may be involved. Conclusions Deregulation or over expression of the MYC onco pro tein is a frequent feature of human cancers, which attests to the pleiotropic role that ectopic MYC plays in cellular function. However, oncogenic MYC can also trigger activation of intrinsic tumour suppressor pro grams such as p19Arf p53, which serve to limit propaga tion of such harmful cells by inducing growth arrest or apoptosis.

While much is known about the mechanisms of MYC functions, the pathways responsible for deciding Inhibitors,Modulators,Libraries the ultimate fate of the cell between life and Inhibitors,Modulators,Libraries death Inhibitors,Modulators,Libraries in vivo are not yet clear. The decision for a cell to become apoptotic depends on the complex interactions of many pro and anti apoptotic factors. Different tissues may exhibit vary ing levels of these factors ultimately determining the fate of a cell. However, tissue specific environmental charac teristics can also affect the interaction between these factors, having a decisive effect on cell fate. The MYC ERTAM transgenic mouse model allows controlled over expression of MYC in distinct adult tissues, SBK and pancreatic b cells, enabling tracking of early changes downstream of aberrant MYC activity.

In this study, high throughput transcriptional profiling was used to identify transcriptional events that may provide clues to explain the disparity in the phenotypic response to MYC activation in SBK and pancreatic b cells. Whilst Carfilzomib expression of genes relating to multiple cellular functions is common to both tissues, expression of genes involved in DNA damage and repli cation is enriched to b cells. Consistent with early increased expression of Ki67 in b cells, key G1 S phase genes showed early changes in expression, whilst G2 M phase genes showed changes at later time points. Down regulation of the cyclin dependent kinase inhibitor Cdkn1b gene in b cells was evident as previously shown in other cell types. The CDKI Cdkn2c, which inhibits Cdk4 and Cdk6, was also down regulated, consistent with G1 S phase transi tion.

The short time period selleck chemicals llc over which these changes were seen supports the idea that MYC induced cell cycle progression occurs through direct activation of key cell cycle genes such as Ccnd1, Ccnd2 and Ccne2. In SBK, although there were changes in cell cycle related genes, the response was much less prominent in comparison with b cells. Gene expression changes included up regulation of Krt6a, Pcna, Ccnd3, Cdk4, and the key G1 S phase cyclin gene Ccnd2, accompa nied by significant down regulation of the CDKI gene Cdkn1b throughout the time course. However, further cell cycle related ge

d hybridization, the amount of driver cDNA was 33 times more than

d hybridization, the amount of driver cDNA was 33 times more than the tester cDNA. As a result, the hybridized cDNAs were eliminated, leaving only the unhybridized cDNAs. The entire population of unhybridized molecules was then subjected to PCR to Diabete amplify target cDNA fragments. Only the molecules of the tes ter sample, which were ligated to the two different adap tors, could be amplified exponentially. A second PCR amplification was performed using nested primers to get a low background, high level enrichment of the differen tially expressed sequences. The PCR products were analyzed by 2% agarose gel electrophoresis. Products from the secondary PCRs were inserted into pMD18 T by T A cloning. The recombinant plasmid DNAs were transformed into XL 1 blue competent cells.

The DNA from recombinant clones was Inhibitors,Modulators,Libraries isolated and sequenced. Bioinformatics analysis All contigs and singlets were annotated according to the GO classification and the hierarchical structure using the Blas t2GO suite. The Blast2GO program, Inhibitors,Modulators,Libraries which assigns the GO terms based on the BLAST definitions, was applied with an E value 10 5. If a transcript was annotated with more than one GO category, it was split equally among them. RNA extraction and RT PCR Total RNA was extracted from the brain using the acid Inhibitors,Modulators,Libraries guanidine method. First strand cDNA was synthesized using 1 ug of total RNA at 37 C for 1 h, with an M MLV reverse transcription system. The primers used to identify of differentially expressed transcripts by RT PCR are presented in Addi tional File 4. The PCR reactions were subjected to 22 26 cycles consisting of 94 C for 30 s, 55 C for 30 s, 72 C for 1 min.

Actin was used as an internal standard. Northern blot hybridization Total RNA from the brain of day 1 2 diapause and nondiapause destined pupae was separated on a 1. 2% agarose gel containing 0. 22 mol L formaldehyde, and transferred to a nylon membrane. Inhibitors,Modulators,Libraries Probes for hybridization were labeled with dCTP using the Random Primer GSK-3 Labeling kit. After prehybridization for 4 h in 5�� SSPE containing 50% formamide, 5�� Den hardts solution, 0. 1% SDS, and 100 ug mL salmon sperm DNA, the radiolabeled probe was added and hybridization was conducted overnight at 42 C. After hybridization, the membrane was washed in 0. 2�� SSPE at 42 C three times and exposed to X ray film overnight at 70 C.

Polyclonal antibody generation and western blot analysis The ORFs of four genes were amplified by PCR, using primers that contained restriction sites. The PCR product was digested by the appropriate restricted enzymes, then purified and subcloned into the pET28a vector. definitely The recombinant pET plasmid was transfected into BL21 cells and induced by IPTG. The E. coli pellet was solubi lized in 6 M urea in 50 mM Tris HCl buffer, pH 8. 0, followed by Ni NTA column purification. Purified recombinant proteins were used to generate polyclonal antibodies in rabbit. Proteins for western blotting were extracted from the brain and SG or the brain SG complexes of pupae, quant

on read this barley, maize and wheat. In fact, two transcript sequences homologous to the wheat thionin gene THI1. 1 were differentially expressed in the cv. Dream after both treatments, but not in the cv. Lynx. Thionins have a general antimicrobial activity against early conidial germination. In addition, a highly inducible expression was observed in the case of the Arabidopsis thionin Thi2. 1 after both fungal infections as well as MeJA treatment leading to an enhanced resist ance to F. oxysporum. Peptidase inhibitors of the defensin family make up the third class of continual up regulated AMPs, represented by homologues of the wheat gene Tad1 and the defen sin precursor PRPI 7 from durum wheat.

While the antimicrobial activity of defensins requires typically complex synergis tic interactions with other AMPs, their promoters are potentially interesting candidates for the targeted and tissue Inhibitors,Modulators,Libraries specific expression of PR and R genes, par ticularly for the protection against F. graminearum in cereal grains. An induction by jasmonates was reported for most of the defensin genes and some of the putative antifungal defensins are reported to be markers for the presence of JA and ET dependent defence signalling pathways. Indeed, indications for an active ET signalling were found in the FHB attacked cv. Dream transcriptome as well. The majority of up regulated cysteine rich AMPs in cv. Dream have shown expression values that were inde pendent of the treatment, but were lower or absent in the susceptible cv. Lynx. It is likely that the majority of these peptides act syn ergistically in a generalized non specific defence provid ing a basal protection.

Inhibitors,Modulators,Libraries AMPs transcribed at a constant level are known key components of an immediate de fence Inhibitors,Modulators,Libraries against invading pathogens, and many pro Inhibitors,Modulators,Libraries teins that are pathogen inducible, for example, in leaves were found to be constitutively present in storage tis sues, such as seed. Moreover, it is generally assumed that genes involved in the quantitative FHB resistance of adapted European wheat cultivars represent such a de fence mechanism. Nonetheless, AMPs can also be part of an induced plant defence. In FHB treated cv. Dream spikes only nsLTP genes were up regulated in response to the disease. Among these Ta. 7843. 1. S1 a at seems to be an interesting resistance candidate, as the gene combines a general high antifungal property with considerable fold change expression ratios at both time points.

Moreover, the putative defensin gene PRPI 7 might be a relevant finding as well due to its possible utilization in a resistant strategy aiming at over expressions GSK-3 of pathogen inducible promoters to directly target the infection sites or the most vulnerable tissues. Such an approach becomes even more inter esting with the recent observation that the biotrophic life form of selleckchem Lapatinib F. graminearum persists in all colonized tissues. Living host cells form a zone surrounding the most advancing hyphae and could be targets for such an ap proach as they allow a

th day 3 and 7 of regeneration may indicate that, in teleosts,

th day 3 and 7 of regeneration may indicate that, in teleosts, selleck chemical this transcript and its protein product may be regulating the process of skin scale regeneration by the induction of cell proliferation. There is also an element of cytoskeletal remodelling via the up regulation of structural proteins and protein degradation. Similarly, as was Inhibitors,Modulators,Libraries noted Inhibitors,Modulators,Libraries with the fasted group, there are a couple of genes up regulated in sea bream, that in humans produce structural problems when defective. Mutations in Dynamin 2 produce abnormally large nuclei in skeletal muscle cells, resulting in muscle weakness, whilst serpin H1 is an essential chaperone in col lagen synthesis, with deficiencies in humans resulting in the premature rupture of placental membranes. As with the 3 days fasted vs.

fasted without Inhibitors,Modulators,Libraries scales compari sons, at day 7 there are up regulated transcripts poten tially linked with mineralization. Such as, caldecrin precursor that exerts a hypocalcaemic activity, decreasing serum calcium, and may indicate that the fish is now actively mobilising calcium for scale mineralization. Real time RT PCR To corroborate the microarray data gene specific qPCRs were performed. In general, the direction of change in expression was concordant between qPCR and the microarray probes and a positive Pearson correlation was obtained between qPCR and probe 1 and between qPCR and probe 2. Despite the good correlation observed between the gene expression ana lyzed by qPCR and the microarray data, a few excep tions were observed, the qPCR fold change for SPP1 transcript in group 3ST, for ColVA2 Inhibitors,Modulators,Libraries transcript in group 3WS, for Col1A1 transcript for group 3ST and for p22phox transcript in group 3STWS vs.

starved group was not correlated with the microarray fold change. The Dacomitinib latter is explained by the high variability found for this gene in different individuals in the experimental groups. The best concordance between qPCR and microarray data is achieved when the observed microarray fold change is between 2 and 7, and in the present experiments the genes analyzed by qPCR which had fold changes closest to the lower limit had lower correlations with the microarray data. Conclusions Fish skin is a very metabolically active organ which has crucial physiological functions, in osmotic regulation and is also an important immune barrier.

Loss of scales and superficial wounds occur in both wild and captive teleosts and the vital importance of integument integrity means damage must be repaired as Perifosine side effects soon as possible. Although several studies exist which characterise tissue and cellular changes underlying skin regeneration in tel eosts, molecular studies have largely been centred on scale formation and calcification, with the latter process not taking place until 14 28 days after scale removal. The study described here, concentrates on the initial stages of scale removal and re epithelialization. Our results show that this is a dramatic process, mainly occurring within the first three days after scal

The reaction of parahydrogen breaks the original magnetic symmetr

The reaction of parahydrogen breaks the original magnetic symmetry and overcomes the selection rules that prevent both NMR observation and parahydrogen/orthohydrogen interconversion, yielding access to the normally invisible hyperpolarization associated with parahydrogen. Therefore the NMR or MRI measurement delivers a marked increase in blog of sinaling pathways the detected Inhibitors,Modulators,Libraries signal strength over the normal Boltzmann-population Inhibitors,Modulators,Libraries derived result. Consequently, measurements can be made which would otherwise be impossible. This approach was pioneered by Weitekamp, Bargon, and Eisenberg, in the late 1980s. Since 1993, we have used this technique in York to study reaction mechanisms and to characterize normally invisible inorganic species.

We also describe signal amplification by reversible exchange (SABRE), an alternative route to sensitize molecules without directly incorporating a molecule of parahydrogen. This approach widens the applicability of PHIP methods and the range of materials that can be hyperpolarized.

In Inhibitors,Modulators,Libraries this Account we describe our parahydrogen studies in York over the last 20 years and place them in a wider context. We describe the characterization of organometallic reaction intermediates including those involved in catalytic reactions, either with or without hydride ligands. The collection of spectroscopic and kinetic data with rapid inverse detection methods has proved to be particularly informative. We can see enhanced signals for the organic products of catalytic reactions that are linked directly to the catalytic intermediates that form them.

This method can therefore prove unequivocally that a specific metal complex is involved in a catalytic cycle, thus pinpointing the true route to catalysis. Studies where a pure nuclear spin state is detected show that it is possible to detect all of the analyte molecules present in a sample using NMR. In addition, we describe methods that achieve the selective Inhibitors,Modulators,Libraries detection of these enhanced signals, when set against a strong NMR background such as that of water.”
“Biochemical strategies that use Entinostat a combination of synthetic oligonucleotides, thermostable DNA polymerases, and DNA ligases can produce large DNA constructs up to 1 megabase in length. Although these ambitious targets are feasible biochemically, comparable technologies for the chemical synthesis of long DNA strands lag far behind.

The best available chemical approach is the solid-phase phosphoramidite method, which can be used to assemble DNA strands up to 150 bases in length. Beyond this point, deficiencies in the chemistry make it impossible to produce definitely pure DNA. A possible alternative approach to the chemical synthesis of large DNA strands is to Join together carefully purified synthetic oligonucleotides by chemical methods. Click ligation by the copper-catalyzed azide-alkyne (CuAAC) reaction could facilitate this process.

Outcome 17-AAG solubility was assessed using Glasgow Outcome Scale Extended score at 12 months. Results The frequencies of deviations from the treatment goals were: episodes of intracranial hypertension 69.5% (of monitored patients), hypotension Inhibitors,Modulators,Libraries 20.3%, anaemia 77.4%, hyperglycaemia 42.9%, hyponatremia 34.6%, hypoalbuminemia 30.8% and hyperthermia 54.9%. Pulmonary complications were common (pneumonia 72.2%, acute respiratory distress syndrome/acute lung injury 31.6%). Thrombocytopenia (4.5%), severe sepsis (3.0%), renal failure (0.8%) and liver failure (0.8%) were infrequent. Twenty-six (19.5%) patients died within the first 12 months due to the head injury. Inhibitors,Modulators,Libraries Age, GCS score, pupil dilation, Injury Severity Score (ISS), ICP?>?25?mmHg, hyperglycaemia and pneumonia predicted a worse outcome.

Conclusions Deviations from the TBI Inhibitors,Modulators,Libraries treatment protocol were frequent. Pneumonia was the most frequent extracranial complication. Age, GCS Inhibitors,Modulators,Libraries score, pupil dilation, ISS, high ICP, hyperglycaemia and pneumonia predicted a worse outcome.
Background Patients discharged from the intensive care unit (ICU) are at increased risk for serious adverse events (SAEs). Recording vital functions and comprehending the consequences of altered vitals on general wards may be suboptimal. This potentially endangers recovery after successful intensive care. We aimed to determine the prevalence of vital dysfunctions after ICU discharge and their effect on patient outcome. Methods A prospective observational study. Adult patients discharged from a tertiary referral hospital ICU to general wards without treatment limitations were visited 24?h afterwards; their vitals were measured and reported to ward staff.

Attending ward nurse responsible for patient was interviewed. Results The cohort consisted of 184 patients who had survived the first 24?h on the ward without complications (age: 57 +/- 16 years; male: 68%). The prevalence of objectively measured vital Batimastat dysfunctions was 15%, and the attending nurse had been unusually concerned about the patient in 19% of cases. Of the 184 patients, 9.8% subsequently inhibitor bulk suffered an SAE. In a multivariate logistic regression model, only vital dysfunctions (odds ratio 3.79; 95% confidence interval 1.18-12.2) and nurse concern (3.63; 1.17-11.3) were independently associated with an increased incidence of SAE. Medical emergency team (MET) assistance was never considered necessary by ward staff. Sensitivity of observed altered vitals on SAEs was 50% and specificity 89%. Sensitivity of nurse concern was 26%, specificity 84%. Conclusions Simple vital function measurement and attending ward nurse’s subjective assessment facilitate early detection of post-ICU patients at risk. The threshold in seeking assistance through MET remains high.

Hydroxylated Skp1 is a substrate for Gnt1 that in turn generates

Hydroxylated Skp1 is a substrate for Gnt1 that in turn generates a substrate for PgtA, and then AgtA, resulting in formation of the pentasaccharide on Hyp143. Mutants lacking enzymes to extend to the trisaccharide state were also unable to sporulate at high O2, suggesting that hydroxylation sup ports extension of the glycan chain to three or more sugars to trigger sporulation. Though the preceding cul mination step exhibited more modest de pendence Inhibitors,Modulators,Libraries on addition of the first two sugars, the more dramatic difference in the static submerged model may simply result from failure to achieve a critical threshold of O2 in the cyst interior. The greater difference was in the role of AgtA, whose contribution was almost as important for culmination as PhyA but was unnecessary for submerged sporula tion.

Thus the role of AgtA appears to be specialized for culmination compared to sporulation. The requirement Inhibitors,Modulators,Libraries of PhyA for sporulation was partially overcome by overexpression of Skp1. This suggests that PhyA action normally promotes Skp1 ac tivity, and its absence can be bypassed by excess Skp1. A related effect was observed on filter development, where Skp1 overexpression inhibited sporulation at high O2 levels that allowed culmination, but removal of PhyA blocked inhibition, indicating that PhyA tunes Skp1 activity. This is consistent with activation of Skp1 poly ubiquitination activity toward an inhibitor. In compari son, the effect of Skp1 modification on culmination im plied Batimastat inhibition of Skp1 breakdown activity toward a hypothetical activator, and the effects on cyst for mation above suggested acti vation of breakdown activity toward an activator.

These disparate effects are consistent with what is known about the SCF family of E3 ubiquitin ligases, which poly ubiquitinate different substrates depending Inhibitors,Modulators,Libraries on which F box protein is present. Furthermore, these Ub ligases can have opposite effects via auto polyubiquitination of the F box protein itself, which results in protection of the substrate receptor. Conceivably, Skp1 modifica tion may selectively affect these different activities. O2 is limiting for Skp1 hydroxylation in submerged culture and mechanistic implications In submerged development, substantial levels of un modified Skp1 accumulated at 5% and 21% O2.

Since i there is no evidence for enzymatic reversal of hydroxylation Inhibitors,Modulators,Libraries or glycosylation, ii the level check FAQ of Skp1 was similar at different O2 levels, and iii Skp1 turns over with a half life of 12 18 h, it is likely that ap pearance of unmodified Skp1 was due to failure to hy droxylate nascent Skp1. Since the total Skp1 pool becomes 95% hydroxylated at 40% O2, O2 is likely rate limiting for Skp1 prolyl hydroxylation. This is consistent with co expression evidence that PhyA is rate limiting for Skp1 hydroxylation.


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Munchkin is on a mission to be the most loved baby lifestyle brand in the world. Their tagline, “It’s the little things®,” refers to all the little things that our products do that add up to make a big difference in parents’ lives.
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Munchkin Nursery Projector and Sound System

Item Weight: 1.34 pounds

Product Dimensions: 5.9 x 5.2 x 6.1 inches

Style: Nursery Projector & Sound System

Size: 1 Pack

Color: White

Item Package Quantity: 1

Number Of Pieces: 1

Special Features: 10 different sounds, 3 different cartridges, voice control, night light

Usage: Depending on your preference

Batteries Included: No

Batteries Required: No

Munchkin Lulla-Vibe Vibrating Mattress Pad
Specifications: Item Weight: 1.01 pounds Color: Blue Product Dimensions: 10.2 x 2.2 x 9.4 inches UPC: 735282153099 Item model number: 15309 Batteries: 4 AA batteries required. (included) Target gender: Unisex Material Type: BPA Free Care instructions: Fabric Care Instructions: To Clean:Wipe with a clean, damp cloth. Allow to air-dry. Do not bleach. Do not iron. Number of items: 1 Batteries required: Yes


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Bert Kaempfert

Bert Kaempfert (born Berthold Kämpfert; 16 October 1923 – 21 June 1980) was a German orchestra leader and songwriter. He made easy listening and jazz-oriented records, and wrote the music for a number of well-known songs, such as "Strangers in the Night" and "Spanish Eyes". He was born in Hamburg, Germany - where he received his lifelong nick… Read More


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Dear Jack: Your Hamster’s New Aquarium

9 years.

Dear Jack,

It turns out, cages sold for hamsters aren’t truly intended for the type of hamster you have. A teddy bear hamster, or Syrian Hamster, is much happier in an aquarium; as opposed to a cage.

So last week, on a random school night, I took you to the pet store to help me pick out a 20 gallon aquarium for Alpha.

Not only is he clearly much happier in the open space, but it is undeniable that you are much more excited to have a pet hamster now!

You are actively involved and excited each evening when we get him out to play.

Plus, it is much easier to clean his home that he predictably pees in the same corner now! Out with the cage, in with the aquarium.

At Age 38, I Am Now the Proud New Owner of a Teddy Bear Hamster (Formerly My 9 Year-Old Son’s Birthday Gift)

Two weeks ago, my wife and I finally cracked on our “no pets in our home, ever” policy, when our son authentically and repeatedly asked for a pet hamster for his 9th birthday.

Being the extremely frugal Jewish-minded parents we are, we cut a deal with our son:

If you buy the cage, we’ll buy the hamster.

So after he went all out and spent $50 on the coolest hamster cage ever, my wife and I dropped the $16 required to purchase a hamster.

Things were off to a shaky start of our son’s pet ownership when as he was attempting to place the hamster in its cage, it bit him on the hand, drawing blood.

I felt bad. So I took it upon myself to hand-tame the 3 month-old hamster.

By Thanksgiving, I had successfully trained the hamster to crawl out of the cage into his hamster ball. Naturally, the rodent and I began to bond.

Throughout the process, I continually invited my son to be a part of the process. He just wasn’t that impressed.

With a 30 day return policy, I began openly talking about returning the hamster. At first, he pleaded against the idea.

But this past weekend, he actually asked if we could return the hamster.

Well… too late.

Because I had already told my wife the day before, “The hamster openly shows me it appreciates me. It depends on me yet I don’t feel taken for granted. I will adopt the hamster as my own.”

I admit, there’s undeniably some psychology in there. I accept that, often, as a parent, you don’t get much verbal appreciation from your kids.

Granted, I’m a grown man. I don’t need confirmation to know I am loved or appreciated.

But the hamster is able to provide something my son is not in this time in his development.

Therefore, the hamster and I now have a symbiotic relationship:

I clean his cage, feed and water him, and provide entertaining exercise for him.

He crawls up to the cage door anytime he is awake and sees me, as if to say, “Hey Buddy, I’m ready to come out so you can take care of me.”

My son named him Alpha. I’m keeping the name. He’s the alpha male of hamsters.

Dear Jack: Your New Pet Teddy Bear Hamster Named Alpha

9 years old.

Dear Jack,

Well, it happened. We did it.

Mommy and I let you get our family’s first ever pet.

You decided on a Teddy Bear Hamster who you named Alpha.

With this being our first week into it, as the nice helpful lady at Petco explained, it’s going to take several days for your hamster to get accustomed to our family.

So each day after school, as a family, we have been letting him out of his cage and so he can get used to us petting his back.

Hopefully soon, we’ll be at the point where you can easily pick him up and hold him.

I think this is a good thing for you!


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Getting Creative with Baby Hacks

As parents of an adorable, busy little toddler, we are always looking for Baby Hacks to make life a little easier or make things happen a little faster. Therefore, we have become pretty creative in our home and during our travels.

The following baby hacks are things that we have come up with (and a bonus found all over Pinterest), that have been working for us.

Read moreGetting Creative with Baby Hacks