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Jessa Kae is a fashion, beauty and lifestyle blogger and somehow manages to juggle that with being a full time fashion and commercial photographer, which she’s been doing for seven years. She’s worked with big-name brands before like Urban Outfitters, American Eagle, Nordstroms, Carbon38, LaraBar, Nume, and JcPenny’s and she loves the creativity and evolving nature of the world of fashion.

Recently, Jessa got the chance to try out several OROGOLD products, specifically the 24k White Gold Collection, which comprises the 24k Classic Hand and Body Cream, 24k Classic Body Butter, 24k Classic body scrub, 24k Mousse Cleanser, 24k Purifying Toner, 24k Aromatherapy Body Oil, and the 24k Deep Moisturizer.

Both Jessa and her partner Jordan fell head over heels in love with this collection. Jessa is a big fan of three-step face washes, and the 24k Mousse Cleanser, 24k Purifying Toner, and 24k Deep Moisturizer combination was one of the best Jessa’s had in awhile. She loved to use the 24k Classic Body Scrub in the shower, and the 24k Aromatherapy Oil was a real treat for her body skin.

All in all, Jessa gave her wholehearted approval to the OROGOLD 24k White Gold Collection, and the fact that she was able to share it with her husband (who, as she put it, is the kind of husband who will gladly sit down and do a face mask with her) makes it even better.

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WTFab from Lucky Community Drawn to the OROGOLD Cosmetics Bio-Brightening Collection

Lucky Magazine is one of the premier online sources for style and shopping. It offers its viewers with expert advice, shopping guides, local advice, product reviews and an interesting contributor community. The Lucky Community is a place where Lucky Contributors can post articles about things they specialize in. The Lucky Community is an exciting place to know more about the latest trends, the best fashion insights, product reviews, the best nail art designs and trendy outfits.

In an article titled “Orogold”, author WTFab reviews the OROGOLD 24K Bio-Brightening Collection. WTFab is one of the most popular fashion bloggers in the Lucky Community and she already has more than 150 articles to her name. In this article, the author states that she was experimenting with her skin care routine to prepare for her upcoming wedding when she came across the OROGOLD Bio-Brightening Collection. She says that she was immediately drawn to the collection because she was looking for products that could help her brighten her skin tone. WTFab reviews the bio-brightening moisturizer and states that this product smoothly glides on the skin and that she loves its scent. WTFab compared the bio-brightening moisturizer to whipped cream and said that after using this collection from OROGOLD Cosmetics, her pores looked smaller and her skin tone appeared to be more even.

OROGOLD would like to thank WTFab for her beautiful review on the 24K Bio-Brightening Collection, one of the best skin care collections for brides-to-be.

Glamazon Diaries and its editor-in-chief Makeda Saggau – Sackey have managed to deliver the latest and hottest trends from the world of fashion along with offering readers with advice on how to look chic. Glamazon Diaries featured the OROGOLD 24K Vitamin C Facial Cleanser and Serum and spoke of how these products gently cleansed and worked wonders on the skin. To read the article by Glamazon diaries, click here.

Vanessa Acosta is a popular beauty vlogger on YouTube who is famous for offering her viewers with exciting product demos and how-tos. Vanessa was particularly excited to demo the ORO GOLD 24K Deep Peeling Mask because it made her skin feel “clear, hydrated and fresh”. Watch Vanessa as she takes you through a step-by-step guide of how to use the OROGOLD 24K Deep Peeling Mask here.

Leslie Presnall brings you an exciting personal style and shopping blog in the form of Southern Flair Blog. Leslie, a teacher by the day and a fashion blogger by the night,  lives in Baton Rouge, Louisiana and covers all sorts of topics on fashion and beauty. Leslie describes how she resisted using the OROGOLD Multi-Vitamin Collection immediately because she was “in love” with its packaging and also portrays why this exciting collection from ORO GOLD has become the highlight of her morning and bedtime routine. Read on to find out how the OROGOLD 24K Multi-Vitamin Collection worked for Leslie.

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Carolyn Anger brings you the perfect destination for all things beauty and style with the Daily Glamourista. Carolyn redefines luxe beauty with OROGOLD Cosmetics and professes her love for the OROGOLD 24K Vitamin C Facial Cleanser and the ORO GOLD 24K Cell Renewal Night Cream. Carolyn tells you why she thought these products to be fab and fell in love with them in the very first night here.

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Michelle Weiss is a highly celebrated blogger at Divalicious Blog and she is famous for her posts on beauty, skin care and makeup. Michelle showcases the launch of the OROGOLD New York Store and talks about four OROGOLD Cleansers – the 24K Bio-Brightening Dark Spot Solution, 24K Bio-Brightening Skin Tone Moisturizer, 24K Oil Control Day Moisturizer Cream and the 24K Multi-Vitamin Deep Peeling. Michelle quotes Lauren Cosenza, the editor of Divalicious Blog, who feels these products were totally worth it because they feel luxurious on the skin. To read more about Lauren’s experience, click here.

AIMEROSE recommends the OROGOLD 24K Bio-Brightening Collection

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AIMEROSE is a fashion, beauty, and lifestyle blog created and maintained by a young lady by the name of Rasa. Hailing from Lithuania, Rasa...

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The Beauty Junkee is a blog written by a dazzling lady named Martha, who seeks to inspire and enrich her readers' lives by sharing...


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Other things that have created havoc with my mojo:

Over the holidays, I learned that I'm going to be a grandmother for the first time. This isn't a good thing. The baby's parents are idiots. I already feel bad for the baby, whose arrival date is supposedly in August. We'll see, I'm thinking more like July. Anyway, if it's a boy, his name will be Oliver Xander, which isn't a bad name, but they plan on calling him OX for short. They think it's funny. :-(

I was contacted late last year by my sister, whom I haven't talked to in 21 years. This isn't a good thing. Long story, lots of drama, suffice it to say, there's a reason we hadn't talked in 21 years. Only a few people know about the dysfunctionality of my family, so for those of you who do, you know that this is not a happy reunion. We've been exchanging emails sporadically, but it's awkward.

Most of you know my father in law lives with us and has for 10 years. He's almost 86 years old now. He has been given a clean bill of health after his latest procedure (well, as clean as the bill can be for someone who has heart disease, prostate cancer and a AAA) and I've been working towards one of his other 5 children to come get him for awhile. This has been a major project this year, as it has entailed MANY doctors visits, scans, tests, surgery and recovery. I love the man as much as any girl could love a father, but I'm tired, and I need a break. I'm tired of feeling like I'm 85 years old and am giddy with the thought of a glimpse of what a 47 year old life looks like. Not just a day here, or an hour there, but LIFE. So, next weekend, one of his other sons is coming to pick him up and take him to HIS home for a visit. It will be an extended visit, two weeks with the son, and then to his daughters house for two weeks. This is a very GOOD thing. :-)

I want to thank you all for being faithful readers and more importantly, FRIENDS. I've been keeping up with all of your goings on through your blogs, and think about you all often. I'm grateful for my "old" friends that still keep up with me also. I love you all and hope to be back to my normal self (such as it was! LOL) in short order. :-)

  1. You will make a wonderful grandma. A perfect grandma. :-)

    Thoughts & prayers are with all of you . . .

  2. You've got so much going on... Sending best wishes and positive vibes, know it's all going to work out...
    hang in there!!

  3. Suzanne, I am sooooooo very glad that you will be getting a much deserved break from FIL duty. My grandfather lived with us for ten years before he had to enter a nursing home and my mom said it almost destroyed their marriage (it was her father). You are a saint for taking it on in such stride.

    A grandma? Gulp.

  4. Glad things are starting to look up for you. I hope spring brings you lots of sunshine and happy moments :) Looking forward to seeing more of the "old" Suzanne and her creations.

  5. I agree with Kristie! Your a saint! I don't think I could do it! As for the baby on they way, maybe it will help the kids grow up, maybe it will turn a light on for them. Hang in there chica! I'm here for ya...you can vent away to me anytime you need too!

  6. This is why I love Spring...new adventures! Enjoy your time away from the FIL :)

  7. I am so glad you are takng a break girl! I hope you are doing some "me time" before pops comes back! I hear ya about dysfuntional families I'll be praying for that and baby on the way. I will be praying that these parents step up! Hang in there and enjoy your well deserved rest girl! ((hugs))

  8. Lots of hugs! Hope you get your peace again.. and well.. congrats on the grandbaby regardless of the situation.. hopefully that will help the parents also.. so due date right after me.. or about same time huh? I'm due July 39th...

  9. Wow girly!!!! You have had lots going on. Maybe we can go shopping for grannie clothes together :)

  10. oh, it has been a crazy 2010 for you. you will be an awesome grandma!!! :)

  11. glad you will get a break soon! I'm sure it is very hard-
    you will be a great Grandma! and you will be happy with that little bundle when it arrives :) HUGS!!

  12. So happy that you will be given your much deserved break! Hope it's filled with fun, relaxation and some fresh air! Hugs!

  13. 2010 has been a rough year for many I know, including me. Hoping yours gets better soon. I'm glad you will be getting a well deserved break. Enjoy!

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Kesha Concert Tickets

Kesha Concert Tickets
Kesha’s debut cd, Animal, was launched in January 2010 to blended views from sound experts. Rolling rock summed the record up as “repulsive, obnoxious and ridiculously catchy.” It premiered at selection one particular from the Billboard 200. Cd track “Blah Blah Blah” debuted when you look at the best ten in america, Canada, Australia and brand new Zealand when you look at the comparable week as cd due to powerful digital product sales.”Tik Tok”, had at this point topped the maps in eleven nations and put many documents in america. “Your Love is My Drug” and “remove it” would observe with premier 10 finishes in the Billboard Sizzling 100 as acceptably. By March, Pet got sold a million copies worldwide using the tracks accumulating an extra eight million downloads. She’d go on to promote a different sort of million records inside upcoming six months. Kesha was showcased on two the greatest 10 singles by Taio Cruz and 3OH!three in Could possibly. Each opportunities came via recommendations by Dr. Luke.

Kesha trip passes

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After that thirty days, Kesha’s previous managers from DAS Communications Inc. filed case, looking for $ 14 million from Kesha and $ 12 million from Luke for commissions on her RCA information price, alleging that she possessed considerable the deadline in order for them to get her an integral record label dedication and squeezed them out-of her profession under great pressure from Luke. Kesha established her own lawsuit in August, motioning to dismiss the problem on reasons that it was her to fire the management when they did not do. She cited the skill organizations operate in an October fit, asking the Ca work Comissioner to declare the woman lasting agreement with DAS void for the reason that it obtained acted as an unlicensed expertise representative whereas “procuring” deliver the results for her in California, by which just accredited agents can “procure” function because of their clients.

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Dave McClure is music business pro, he works and writes articles about low priced occasion passes including Kesha violation for 5+ years.

Kesha seats

Kesha tickets

Kesha’s debut cd, Pet, premiered in January 2010 to combined reviews from sound critics. Rolling rock summed the cd up as “repulsive, obnoxious and ridiculously catchy.” It premiered at variety 1 on Billboard 200. Album track “Blah Blah Blah” debuted when you look at the major 10 in the usa, Canada, Australia and brand new Zealand in the same few days due to the fact cd as a result of effective digital revenue.”Tik Tok”, acquired currently topped the charts in eleven nations and set countless information in the usa. “Your like is My Drug” and “to take wax off” would adhere to with leading ten finishes on the Billboard Hot 100 as perfectly. By March, Pet got sold a million copies around the globe because of the paths accumulating an more eight million packages. She would go on to market yet another million records when you look at the future 6 months. Kesha ended up being showcased on two best 10 singles by Taio Cruz and 3OH!3 in might. Each opportunities got right here via recommendations by Dr. Luke.

Kesha tour tickets

Pet is of this dance-pop style; it includes elements of electropop with its production. It ranges from excessive energy pop music party paths, to electronic ballads, to tunes with guitar riffs. With regards to came to the woman ballads, Kesha pointed out which they “would nevertheless have that 4-on-the-floor to retain the heads moving.” She also regards the desire to have her reside reveals becoming “rowdy and absolutely exciting, and ideally dance-inducing.”

After that month, Kesha’s former supervisors from DAS Communications Inc. submitted a lawsuit, in search of $ 14 million from Kesha and $ 12 million from Luke for commissions on her RCA papers package, alleging that she obtained extended the deadline to allow them to get her a crucial document label agreement and squeezed them from her work beneath tension from Luke. Kesha established the girl own lawsuit in August, motioning to discount the way it is regarding the grounds it was her straight to fire the administration when they didn’t execute. She cited the Skill Businesses Act in an October go well with, asking the California work Comissioner to declare the woman cope with DAS void since it found acted as an unlicensed skill agent even while “procuring” function on her in Ca, wherein only certified representatives can “procure” give great outcomes for customers.

Kesha presented a benefit live concert on Summer sixteen, 2010 only in which all profits went along to help sufferers of this may 2010 Tennessee floods from her hometown Nashville. She increased close to $ 70,000 through the occasion. She also donated one,000 pounds of dog meals to an area dog housing for pets abandoned in flood. She ended up being a supporting focus on the summer season us knee of Rihanna’s last woman in the world tour. Pet is supposed to be re-introduced beneath the name Animal + Cannibal on November 22 aided by the brand new songs offered as a different long play, Cannibal, as well. Lead solitary, “We roentgen Who We R” grew to be just the seventeenth track to debut towards the top rated regarding the Billboard Scorching 100 three days ahead of the album’s launch.

Kesha makes use of a sing-track rapping design on a selection of her songs, which she admitted discovered begun as a joke, most notably on her behalf first solitary “Tik Tok”. The brand new York Instances indicated that the tune represented “the complete and painless assimilation of white female rapper into pop songs.” The la Moments in contrast this vocal fashion compared to that of L’Trimm and Salt-N-Pepa. Kesha has-been criticized for making use of Vehicle-Tune and vocoders to distort the woman vocals on her behalf record Pet. Billboard talked about that the heavily prepared vocals “designed it hard to tell whether or not Kesha could actually sing.” You will see Kesha offered tickets from on line tickets agent

Dave McClure is songs business expert, he works and writes articles about low priced occasion tickets including Kesha concert seats for 5+ years.

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Mavericks Tickets

Mavericks Tickets
Available completely reveal diary of events, thus discovering next Mavericks games and buying the specified Mavericks tickets. In addition, you can search for Dallas shows and purchase the tickets essential for these also. So, you see, it is possible to plan your personal future activity possibilities without needing to move through the front side of one’s computer and simply with a few clicks from the mouse.

For folks who work all day every day, it may be quite difficult to steadfastly keep up aided by the Mavericks and their games. Therefore, it variety of is practical to find the Web in order to find Mavs passes. The bonus provided is Mavericks seats tend to be provided if you wish, to be able to select the event to deal with without the issues. Similar applies to people who wish purchase Dallas concert tickets. How will you purchase a ticket unless you understand once the shows are happening? In this manner, you can notify your self and determine the Dallas concerts as you are able to in fact visit. Plus, you can see which musicians tend to be performing and choose to see some one that is your chosen for a tremendously long period of the time.

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Oklahoma Tickets

Oklahoma Tickets

There are specific internet vendors that provide you with the Oklahoma passes that you want, at discounted costs. Therefore, if you would like buy Oklahoma Thunder passes or you are looking for Oklahoma concert tickets, the good news is that you don’t have to go out of the home. It is possible to store, including, for Oklahoma City Thunder tickets, from the comfort of your own home.
Cyberspace is rolling out tremendously before few years, the internet environment gradually becoming an expansive marketplace for numerous services and products. These days, if you want to buy Oklahoma Thunder seats, you might be certain that the world-wide-web and its specialized sources will get the finest price. On the web, not just you’ve got the possibility of purchasing Oklahoma City Thunder tickets with months in advance, but you can in addition stay easily, realizing that you have paid a realistic price for such purchases. The same is true of Oklahoma concert seats, or just about any other type of Oklahoma tickets for that matter.
Frequently, cyberspace is perceived as decreasing option, as everybody knows that there are particular Oklahoma baseball passes being hard to find. And, when we tend to be speaing frankly about Oklahoma show seats, we’re able to definitely state that things can be equivalent. For every of occasions held in Oklahoma, one can find information available on the internet, for instance the exact day and area. In this manner, you can purchase the Oklahoma Thunder seats that you would like and start to become very sure you are free that day of the event.
If you like recreations and particularly basketball, then you will not overlook the opportunity to get your very own Oklahoma City Thunder seats. Obtaining the tickets is very simple than you imagine and you have all the details that you want to know over the Internet. Let us state that you would like to purchase Oklahoma baseball seats when it comes to Oklahoma City Thunder-Memphis Grizzlies game. Well, things cannot be less complicated. You click seats and you can look at sitting chart, choosing the seats that you would like.
Keep in mind that, when selecting passes, often there is the chance of being sold out. You will find a finite number of Oklahoma Thunder seats, so you may like to hurry and get your personal admission before they all are sold-out. In the long run, it surely doesnt matter what type of Oklahoma Thunder seats you wish to purchase. You can search for Oklahoma City Thunder passes or simply equivalent, would like to get Oklahoma show seats. If there are a great number of those who are baseball or music followers, then need may be as high. Given such explanations, it is possible to definitely why experts recommend getting your Oklahoma tickets in advance and never leave it regarding final possible minute.

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Miami Heat Tickets

Miami Temperature Tickets

Miami Heat Basketball group: an expert baseball staff is one of the seven groups inside Atlantic Division regarding the Eastern meeting for the National Basketball Association (NBA). Heat plays in United states Airlines Arena in Miami, Florida, and wears jerseys of red, gold, and white. The history of Miami Heats starts back in 1988, whenever Miami temperature was only an expansion group and did not make their mark until long until the staff’s fortunes changed during mid 1990’s.

With inclusion of talented players like center Alonzo Mourning and point shield Tim Hardaway towards the staff, Miami Heat made a stronger come-back when you look at the NBA standings. With a supporting cast of Jamal Mashburn, Voshon Lenard, and P.J. Brown, the Heat rose toward Eastern Conference Finals in 1997.

The Miami temperature got hold of Shaquille O’Neal for Odom, Brian give and Caron Butler via trade because of the l . a . Lakers therefore the refurbished staff caused it to be into Eastern meeting Finals, in which they destroyed in seven games to your Detroit Pistons.

NBA Miami Heats, in the long run allow us into among the best groups on NBA circuit. With astounding people associated with the stunning team, Miami Heat is probably the top-ranked teams in NBA. Heat made their particular first on November fifth losing to the la Clippers 111-91 at the Miami Arena while the followers needed to wait a while for The Miami Heats very first winnings as they got to an awful begin, losing a NBA record 17 games in their inaugural season. Eventually, heat got their particular very first winnings beating the Clippers in LA 89-88 in a nail-biting match. NBA baseball Miami Heat got their first home win 5 games later on, when they overcome the Utah Jazz 101-80. Joy and celebrations rocked the roads of Miami due to the fact home staff, at last, produced something that the fans could be happy with. The fire held burning in the warmth as it revealed improvement despite finishing in final destination with a 24-58 record within the next period.

The succeeding 12 months proved really successful for The Heats under new Miami Heat Coach Kevin Loughery as the temperature got their particular very first taste of achievement into the playoffs on the last day of the growing season by finishing in 4th destination with a 38-44 record. The 1993/94 season ended up being led by the scoring of Glen Rice, the shooting of Steve Smith, in addition to rebounding of Ronny Seikaly that made temperature to improve its place, within their very first previously winning season. The 1995/96 period had been the season of restructuring associated with the Heat while they acquired Alonzo Mourning, Pete Myers, and LeRon Ellis through the Charlotte Hornets at the time before the start of season. Nevertheless fortune had not been to their side while they struggled for quite some time getting right back on track.

Heat cruised for their second straight Atlantic Division name with a solid 55-27 record in 1997/98 season. The warmth began the latest Millennium with a brand new arena and an innovative new style. In the first game at The united states Airlines Arena, heat started out with a 111-103 conquer the Orlando secret. In their new house, The Heat proceeded to try out strong defensive baseball as Alonzo Mourning won their 2nd straight Defensive Player of the season honor leading heat to a 4th right division subject with a good 52-30 record. When you look at the after injury-hit period, NBA Miami temperature persistently played powerful basketball, publishing a 42-27 record, and Heat proceeded to complete in 2nd spot with a solid 50-32 record. 2002/03 season ended up being a dreadful 12 months as the Heat finished eventually destination with an awful record of 25-57. The recent 2004-05 months marked a season of reunion the TEMPERATURE, as four previous Miami temperature people and mentors rejoined the team for a push for an NBA tournament. Ron Rothstein rejoined Miami in September as an assistant advisor.

The Miami Heat Basketball group is an excellent blend of old players with fresh talent. A team which arises to expectations of their million of fans that follow Basketball keenly. With passage of time, the group has actually enhanced tremendously now it has a powerful placement in NBA Circuit. To find out more about Miami Heat Tickets check out:

Macie is a staff journalist for Ticket Nest ( and enjoys writing about her vacation, movie theater and concert experiences. She can be reached at

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New Reader?

First of all a warm welcome to Our Beautiful World! We are Kirsty, Ros, Mandysea, and Riitta, Blog buddies from different parts of the world.

The blog is an initiative by Kirsty. We want to build a friendly community where you can share the beauty of the world as seen through your lens.

On Our Beautiful World you'll find photo inspiration, tips for blogging and photo editing, and weekly photo linky parties with a theme. We'd love it if you join the parties and share your tips and tricks with us!

Since this is not a challenge blog, what we'd really want is that we all share photos with each other from daily life to seasonal events around the globe and share this through linky parties. It's not about who-does-best. It's all about inspiring each other, learning from each other, and sharing the beauty around us.

Take a photo according to the prompt each week (regular photography, photoshopped photos, instagram, lomography, etc all welcome!), come back to this blog and link up with the linking system. Then hop around to see what others have to offer in their part of the world. It's interesting to see what life is about in different corners of the world, so we can't wait to see your photos!

Please post only a link to your entry, not to your entire blog.

When you link up your project, don't forget to tell us in which part of the world were those photos taken. You know sometimes it's hard to tell from the picture. When you type in your name using the linking system, simply put your country (or city) in bracket after your name, e.g. Alice Watson (Canada).

Weekly prompts will be posted each Sunday. If you have questions, feel free to ask!

Now, we don't have rules here, but there are some things we would like to ask of you.

First of all you're not obliged to visit all the blogs of the people who joined the party of course, but we do ask you to visit at least three others and leave them some blog love. That's why linky parties are so much fun and what they're generally are all about, aren't they?

Second, please link up only with photos that are according to the prompt / theme of that week. If your link has absolutely nothing to do with it, then we might have to delete your link from our party, which we won't like to do at all. AND please.... do not promote your own link parties or giveaway here, this is not a place for that kind of stuff!

Third, please grab our OBW button found at the top of the side bar, give notice in your blogpost of our linky party and give your readers a link to our blog so they can share in the fun as well. If you can't even mention OBW on your blog post (please don't ask us to click on a link you have and lead us to hundreds of challenge blogs you play along from Monday to Sunday), we might have to take you off the party.

We would also love it if you share the story behind your photo(s). Do feel free to share multiple photos in your blog post, but please link up the one photo that you think most appropriate for our word prompt.

And last, it is not necessary but would be totally cool if you are able to share some information from time to time about what camera and lens you use, settings or photoshop information etc.

Have fun!!

Love, us


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Monday, 25 August 2014

Stitched and Bound Exhibition 2014 - Back to the Razor's Edge

A couple of weeks ago Miss M and I visited this exhibition which is held by the West Australian Quilters' Association (WAQA) biannually to "...encourage individualized expression and excellence in contemporary quilting." (Stitched and Bound 2014 catalogue)

This year was the 10th exhibition in the series and there were some very inspiring pieces. My favourites included...
'Green Corridors' by Stephanie Knudsen
'Mysterious Outback - Bowl of Plenty' by Jennie Abbott

'As Ye Sow We Hope Ye Will Reap' by Taryn Blight

'Munda Biddi Sojourn' by Meg Cowey
Miss M studies the various stitching techniques

Some of the pieces made political statements, others commented artistically on nature and/or events that impact heavily on communities, while another reflected on the 'public' vs 'private' face of men.

'Sovereign Borders' by Virginia O'Keeffe
'Out of the Ashes' by Pamela Manika

'Honouring Good Men' by Louise Wells
There were also a few pieces from the inaugural exhibition included. All in all it was a lovely, very inspiring morning.

I hope you find something to inspire your creativity this week.

Sunday, 17 August 2014

FNSI, a Finish & a Secret Project

After a very interrupted sleep on Thursday night, thanks to Miss M, & a trip to Lancelin for lunch with my sister on Friday I was too tired to stitch on Friday night. I was, however, with everyone in spirit & flicked quickly through the latest Patchwork & Stitching magazine which has some wonderful projects in it.

Congratulations to Wendy Bertello (Sugarlane Quilts) and Anorina Morris (Samelia's Mum) for each having a project included in the magazine. They are lovely projects and you ladies should be quite proud of yourselves.

I did manage to do some stitching on the binding of my crazy patchwork Christmas placemat (see a pic here) in the car on the way to a family lunch in Mandurah yesterday though. It has to be handed in to Community Quilts on Tuesday so I have to get moving to finish it off!

You may have seen the froggy fabrics I had for a project in an earlier post. This project is finally finished and I'm very happy with it. Just have to find somewhere to hang it now.

Pattern is 'Lucky Frogs' by Helen Lownds from Pathwork & Stitching Vol 14 No. 4

I've also nearly finished a secret project I've been working on for a friend. I can only show you a sneak peak right now but I'm very happy with how it has turned out.

If you would like to see what everyone else got up to for FNSI you can check out Wendy's blog here. I hope you all get very inspired and have a wonderful week.

Saturday, 2 August 2014

Unfortunately I didn't manage to get any stitching done last night as we didn't get back from dinner until late. I did flick through my new 'Love of Quilting' magazine while watching some of the finals of the Gymnastics in the Commonwealth Games though. I do love watching the gymnastics!

I also heard, after doing my post yesterday, that I was lucky enough to win 2nd prize in a raffle at the craft fair yesterday. I'll post a pic after I collect it later in the week.

Hope everyone else had a productive night. If you'd like to see what they all got up to you can check it out here. Thanks again for hosting us Cheryll.

Beautiful sunset over South Perth in May - Aren't the colours just gorgeous?
Have a great weekend!

Friday, 1 August 2014

My Poor Credit Card

... needs a well earned rest after the workout it had at the WA Craft Show today. I admit to being both an impulse buyer and a Christmas-holic, and my good "friend" Phil egged me on at Logan's Patchwork stand with the beautiful Christmas panels and fabrics they had for sale today. I'm not complaining though, I picked up some lovely pieces. Thanks Phil :)

Beautiful Xmas panel from Logans - luckily the red/green & black/gold
ones had sold out or they would have come home too!

Xmas panel #2 - also stunning!
Xmas stocking from the Teddy Tree for Miss M, stocking advent calendar &
Xmas fabrics from Logans, & the beautiful blue fabric from Logans because
I couldn't resist!
I also bought some essentials...

Thread organiser, bobbins, sewing machine needles, an
applique mat & a hand-crafted wooden needle case

... and some gorgeous threads & bits & pieces.

Thread packs & Angelina fibres from the Thread Studio, owl applique packs
from The Quilt Fairy, Winnie the Pooh fabric book from the Teddy Tree

Finally, I also picked up a few Xmas presents for family and friends. The WA Craft Show included the WA Teddy Show and was paired with the WA Wood Show. One of the Wood groups/Guilds (I can't remember which one) always has some lovely small items available for sale - that's where the needle case pictured above came from.

Wooden toys for my nephews, an Irish rolling pin for a friend, cute owl ornament
for Miss M's friend, jelly roll & red/black fabric pack for some wonderful ladies

We were also treated to 2 wonderful exhibitions - the Bunbury Quilters 2014 International Challenge (nautical theme) & the Southern Cross Quilters exhibition. There were some absolutely awesome pieces on display.

I also got the chance to meet Australian Quilt Artist, Caroline Sharkey, and have a chat to her about how she does her quilts. Her work is so inspiring! She is coming to Perth to run a workshop next year but unfortunately I won't be able to attend. I'm hoping to try out one of her online courses soon. If you'd like to see who she is & what she does, her website is here.

The shows run all weekend so if you get a chance I highly recommend going along.
I'm hoping to join in with Friday Night with Friends tonight. If you'd like to participate too, jump on over to Cheryl's place and sign up here.
Have a great weekend everyone!


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Sorel Women’s Out N About Felt Moc

SKU: 170233. Categories: , .

A comfy slipper moc you can wear just about anywhere. Premium suede upper with rich felt vamp is lined with cozy fleece and enhanced with a removable EVA footbed, then finished with a vulcanized rubber outsole whose herringbone design delivers traction on wet surfaces.

The Construction

UPPER: Suede upper with felt vamp. Fleece lining.
FOOTBED: Removable molded EVA footbed. Fleece topcover.
MIDSOLE: Vulcanized rubber.
OUTSOLE: Rubber outsole with herringbone design. Vulcanized rubber compound is improved for wet traction.

  • Shaft Height: 4 in.
  • Heel Height: 3/4 in.
  • Platform Height: 3/4 in.
  • Weight: 8.5 oz / 242 g. (Measurements based on size 7)


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Tuesday, September 13, 2011

A Dress

 I made a dress!  My brother-in-law got married in Snoqualmie, WA in July, so I decided to make a dress rather than deal with shopping for one.
It's the same dress that I made at the Heather Ross Weekend Sewing Workshop this past January.  Except I never finished that one so......  I figured I'd make a new one.   I had just sent my deposit payment to Heather Ross for the WSW in Palm Springs for next January.  I was all pumped and wanted to make something.

  It's Anna Maria Horner's Evening Empire Dress for the bodice and the skirt panels from Heather Ross' Weekend Sewing (Yard Sale Skirt).  Rather than line fabric with interfacing, I chose to go with some AMH home dec fabric for the top.  The bottom is a light yellow linen/rayon blend.
I added 3 small pleats to the back to keep the skirt seams symmetrical.

I actually finished the dress the night before the wedding and with all the seam ripping and re-sewing, the dress bodice ended up a little floppy.  I am on the fence as to whether or not this was appropriate to wear to a wedding.  It seems to have come out a little to "summer dress" than "wedding appropriate."

The inside is lined with some Good Folks.

The zipper install came out okay.....

I moved the straps out a lot!

I made some bias trim to go around the bottom.
 I really like how it turned out.

:) Raven

Tuesday, August 23, 2011

A Finished Quilt (finally!)

This quilt has been a long time coming!  Most of the blocks were finished last May/June.  I started out making squares in squares with varying widths of strips.  My plan was to cut all the blocks up and "bento" them back together.  I guess I sort of got a bit lazy and also like the way the blocks looked slashed in half.  I kept some of the blocks as squares and sewed them together.  I quilted half of it in November and the other half in December.  Binding was sewn on in January some time.  And, since we also got rid of our cable box in January, there was no hand-sewing-while-tv-watching going on, so it took until a few weeks ago to finish stiching the binding down on the back.

  It's been on the couch.

Most of the prints are from Hope Valley by Denyse Schmidt.  The solids are all Kona except for the taupe color which is a Kaffe Fassett Shot cotton.

The back is AMH Flannel.  Flannel for a quilt back is awesome.  This print went so well with the colors from the front!   Quilted all over stipple.

Tuesday, August 9, 2011

Farmer's Wife Sampler Quilt- Week 5

Block #9 and #10

#9- Box
This block's colors are my favorite!  I placed the green print fabric on so many different blues
 and ended up with this one.  I love how it turned out!!
#10- Bowtie

     I tried paper-piecing a few of these blocks.  I'm sure if I were more comfortable with paper piecing, I would prefer to do it.....but I think I like cutting the templates.  I've been using the templates and cutting about 1/4" larger that required.  After that, I piece and trim and piece and trim.  It's working out better for me, but for the life of me, I cannot get my points to line up.

It is so fun to see how different all these blocks can be, just by changing up the fabrics!
If you haven't - check out the flickr group - some seriously amazing quilts are being made!

Friday, August 5, 2011

Farmer's Wife Sampler Quilt - Week 4

Block # 7 Birds in the Air
 Wow- this block was so insane-driving!!  Those points meet up only in my dreams!!
But oh my goodness I flippin love these colors together!!
Block #8  Bouquet
Not sure what happened at the bottom.....I'll trim them up later.....
I'm still undecided on the dark blue....
I need to lay all the blocks out together and see how they play off each other.

Another week done!

I would describe myself as a quitter.  This QAL is a test for me- to see if I can push through my wankiness and whining to actually complete an incredibly labor intensive quilt.
So far - so good!!

xo, Rave


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Cray Lands $36 Million Contract to Upgrade Weather Facility Supercomputers

Posted by on Jan 26, 2016 in Uncategorized | No Comments

Our client Cray just signed a $36 million contract with the European Centre for Medium-Range Weather Forecasts (ECMWF), to deliver new XC40 and XC40-AC supercomputers later this year.

ECMWF specializes in global numerical weather prediction for the medium range (up to two weeks ahead), and also produces extended-range forecasts for up to a year ahead, with varying degrees of detail. The center uses advanced computer modeling techniques to analyze observations and predict future weather. Forecasts are produced and sent out twice daily to Member State national meteorological services for their use. Source: Scientific Computing.

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OWTK Kid's Music Monthly Podcast

Welcome to the OWTK Kid's Music Monthly Podcast! Sit back, relax and revel in the Golden Age of Family Music.

Jun 1, 2013

School is out this month for most -- yay summer! Enjoy new music from Key Wilde & Mr. Clarke, Joanie Leeds, Sugar Free Allstars, Princess Katie & Racer Steve, and more while running through a sprinkler or hitting the open road for a family adventure. Read more about all of the bands played this month on


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How can governments raise money from automation and ICT to compensate the losers?

March 20, 2017

If we want to innovate, we need to disrupt our relationships and embrace tension

March 20, 2017

Links I Liked

March 20, 2017
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Either a genius subeditor placed this ad, or serendipity is a wonderful thing [h/t Mark Perkins]duck

Branko Milanovic at his brilliant best on why 20th Century tools cannot be used to address 21st Century income inequality, and what to do instead

Edible drones are a thing, apparently, or soon will be. 50kg of food and soon you’ll be able to eat the vehicle. Here’s the inedible version

Cash v Chickens. Chris Blattman v Bill Gates. What’s not to like?

Ten top tips for social scientists seeking to influence policy. Some good advice here

Useful discussion of likely next steps on proposed US aid cuts

Oh dear. The first meeting of the first “Girls Council” in Saudi Arabia [h/t Saudi girls councilRana Harbi]

Chinese inequality is now falling.

As women in India get better educated and (a bit) wealthier, they stop going out to work.

What if it had been a woman being interviewed in South Korea? Wonderful satire on women, multi-tasking and a man with only one sock (wait for the end)

‘Reform for y’all’. Chinese state rap videos. Really. Not sure I can ever forgive Ranil Dissayanake for this link

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'LDC(s)' can also refer to...

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Least (formerly less) developed country or countries, of which 50 have been identified for the purposes of monitoring and coordinating the various U.N. programs of support under the OHRLLS. See ...

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Max And The Broadway Metal Choir

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‘The Devil gave us Death Metal and God gave us Max’: this was the publicity that introduced Maximilan Gelt - a Jewish delicatessen owner from Miami, Florida, in his late ...

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n. a nonmetallic element. Phosphorus compounds are major constituents in the tissues of both plants and animals. In humans, phosphorus is mostly concentrated in bone. However, certain phosphorus-containing compounds – for example adenosine triphosphate (ATP) and creatine phosphate – play an important part in energy conversions and storage in the body. In a pure state, phosphorus is toxic. Symbol: P.

Subjects: Science and Mathematics — Medicine and Health.

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(subclass Archosauria, order Thecodontia)

A suborder that has only one family, the Phytosauridae, which were specialized thecodonts similar to crocodiles in general shape. They date from the latter part of the Triassic and inhabited N. America, Europe, and India. They were displaced by the crocodiles at the end of the Triassic.

Subjects: Zoology and Animal Sciences.

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Battle of Pontvallain

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Bertrand du Guesclin (c. 1320—1380)

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The battle of Pontvallain took place on 4 December 1370 in the department of Sarthe, in the arrondissement of La Flèche. It followed Robert Knolles’s chevauchée that devastated Picardy, Champagne ...

Subjects: Military History.

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Pope's garden

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Alexander Pope (1688—1744) poet

Lord Orford Horace Walpole (1717—1797) author, politician, and patron of the arts

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Twickenham, London, England, was made by Alexander Pope from 1719 when he bought a Thameside house and turned it into a Palladian villa. All that survives of the garden is ...

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Bill Potts

(b. 1928)

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Lester Young (1909—1959)

Count Basie (1904—1984) American jazz pianist, organist, and bandleader

Miles Davis (1926—1991) American jazz trumpeter, composer, and bandleader

Quincy Jones (b. 1933)

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B. William Orie Potts, 3 April 1928, Arlington, Virginia, USA, d. 16 February 2005, Plantation, Florida, USA. Potts displayed musical ability from an early age, playing Hawaiian guitar and accordion ...

Subjects: Music.

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Precision Dancing

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music hall

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Defined as group chorus movement whose main appeal is exactness of performance, precision dancing employs simple steps because the emphasis is on the overall geometric effect of the dancing rather than the individual dancer.

Subjects: Dance.

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The presecretory precursor of a polypeptide or protein hormone, which contains the N‐terminal signal sequence, the sequence of the hormone of interest, and any other sequence. Examples are: preproglucagon, preproinsulin, preproparathyrin, prepro‐VIP.

Subjects: Chemistry.

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primary audience

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Targeted group for some form of mediated communication, such as for an advertising campaign or a television channel, defined by demographics and/or lifestyle (see alsotarget audience). See alsodual audience; comparesecondary audience.

Subjects: Media Studies.

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rape crisis center

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A community service agency usually staffed by volunteers, perhaps a social worker, a psychologist, and a nurse, with facilities for the care and treatment of victims of rape and sexual assault. They may function primarily as hotline telephone access crisis centers with referrals to relevant specialists but preferably have on-site staff constantly available, and some also have outreach services.

Subjects: Public Health and Epidemiology.

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The power of an embryo to continue normal or approximately normal development or regeneration in spite of experimental interference by ablation, implantation, transplantation, etc.

Subjects: Chemistry — Biological Sciences.

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Andrew Akiba Shonfield

(1917—1981) political economist

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Shonfield was born in Tadworth, Surrey, 10 August 1917, the fifth of seven children to Rabbi Victor Schonfeld and his wife Rachel Lea (née Sternberg). He died in London 23 January 1981. Both his parents were Hungarian-born, and his early education combined orthodox Judaism and Talmudic study, albeit leavened with a central European cosmopolitanism. He then won a foundation scholarship to St Paul's School and from there proceeded to Magdalen College, Oxford, graduating in the second class in philosophy, politics and economics in 1939, a result in no sense reflecting his emerging gifts as an economist but much more the inevitable consequence of time so assiduously spent in Oxford left-of-centre student politics. He made a number of close friendships during this period, including with some such as Tony crosland who would go on to become leading figures in the Labour Party. It was here too that he began to forge his own distinctive Brand of internationalism and his dirigiste but non-partisan approach to government in the economy.

From The Biographical Dictionary of British Economists in Oxford Reference.

Subjects: Economics.

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Reynolds Stone

(1909—1979) engraver and letter cutter

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 (1909–79) English wood engraver (see woodcut illustration), designer, painter, and letter cutter.

Stylistically a descendant of Bewick, and, in lettering, of the Renaissance masters of calligraphy, Stone worked extensively in ...

Subjects: Bibliography — Art.

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wave clouds

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Clouds occurring in an air stream characterized by wave motion, usually after passing over a mountain barrier. Lenticular (lens-shaped) clouds and cloudlets appearing stationary in the crests of the waves are a characteristic sight in climates experienced on the lee side of mountain barriers, e.g. in eastern Scotland and Sweden.

Subjects: Meteorology and Climatology.

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Friday, April 09, 2010

Red Dead Redemption - Multiplayer Free Roam

Rockstar Games have just released their first video showing multiplayer action for Read Dead Redemption.

Check out first impressions of the multiplayer game play at IGN, 1UP, GamesRadar and GameSpot.

I'm counting the days until this is released.

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Knowledge is Power

In an effort to make a difference in this world, many people gathered last night at the Fashion Institute of Design and Merchandising for Room to Read's 10th anniversary. Local designers were invited to create wearable art t-shirts with the theme "Literacy and Gender Equality". Designers had two things in common: a plain white t-shirt and a desire of helping kids in developing countries. For my creation I used ordinary materials like pencils and alphabet blocks to create an outfit that reminds us of the royalty. My idea was to make people realize how powerful women can be if they have the same opportunities as men. At the end t-shirts were auctioned off and 100% of the money goes to Room to Read's programs of education in 3rd world countries. Such an honor to be part of this great cause!

1 comment:

  1. I like the blue color of the dress, and you've got beautiful legs. fit's perfectly.


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Junior Lessons
Adult Lessons
Wind Surfing
Power Boating
Team 15
Calendar of Events
Online Booking
The Barn
Level One is an introduction to the sport aimed to you afloat safely.

Level Two is about getting competent to handle a boat by yourself in light winds.

We offer all levels of the scheme from novice to racer trying to go faster. This year we will be running half-day courses over each of the main holiday weeks during Easter, Whitsun and the summer.

The real changes in the scheme come at Level 3, now called better sailing. The aim is to bridge the gap between level 2 and the advanced modules. We try to use a variety of boats and maximise time on the water which should give your greater confidence to sail without the instructor on board in light and moderate winds.

Advanced modules       These courses are designed to run over two days, we are happy to run them over five half days which should give a better range of conditions.

Seamanship Skills :

These remain at the core. They should enable you to handle your boat in a variety of conditions and solve problems afloat.

Day Sailing :

This is a new strand designed to equip you with the skills and knowledge needed to take your boat cruising.

Start Racing :

Are you the competitive type? Start racing will give you enough knowledge to join in with your local club racing. It's the first step of the Olympic ladder!
Adult Sailing Lessons in Cornwall

Sailing with Spinnakers

As the name suggests this course is about getting the most out of all 3 sails on your boat. It covers either symmetric or asymmetric spinnakers.

Performance Sailing :

This is the adrenalin rush end of the sport. The course can be taken in your own boat or one of ours. Getting the most out of these boats requires special techniques: the coaching on this course should help you master them, stay upright and blast off!
A full season's experience would be the recommended minimum before taking this course.

Contact E-Mail: bob@paddleandsail.com


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Rhythms of the River

2014 September/October Edition

Photos courtesy of the McCracken County Public Library

A living, boiling, bubbling, baking history of a time long ago in Land Between The Lakes

2014 September/October Edition

What magic exists in those heirloom recipes from our great-grandmothers that just reading them connects us into family and culture and time as poetry might? In making the recipe we can physically smell and taste what once kept a family going.

Can the Coke Plant be Saved?

Exclusive Online Content

This article first appeared in the Jan/Feb 2010 issue of Paducah Life Magazine.

Paducah is a city of landmarks. From Whitehaven to the Market House, buildings teeming with history dot our landscape. One of the most noticeable and iconic of them is the Coca-Cola plant in Paducah’s mid-town community.

The Coke Plant

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This article appeared in the Jan/Feb 2010 edition of Paducah Life Magazine.

What is the significance of Paducah's Emancipation Celebration Date?

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Reverend W.G. Harvey: One Man's Journey on the Road to Civil Rights

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The Emancipation Proclamation, Abraham Lincoln’s executive order freeing slaves in the United States, went into effect January 1, 1863. Yet over a century later, full freedom for black Americans was not yet realized.

West Kentucky Wanderings - Princeton: A Bloom in the Black Patch

2014 May/June Edition

Carson Park: A Gift for the Ages

2014 May/June Edition

Luther F. Carson, founder of the Paducah Coca-Cola bottling plant, knew something had to be done. The McCracken County fair lost its home in 1923 after a property sale, and ever since then, became itinerant.

Gettin' Ready for the County Fair

2014 May/June Edition

Every year when summer comes around, they stretch a banner 'cross the main street in town. You can feel something's happenin' in the air ... getting ready for the county fair. -Bruce Springsteen

The Devil & Mr. Cobb

2014 March/April Edition

March 11 marks the 70th anniversary of the death of Paducah's own Irvin S. Cobb, and by the time of his passing he had amassed a stunningly prolific and varied resume: author of over 60 books and 300 short stories, newspaper columnist, World War I correspondent, screenwriter, actor in 10 films, and host of the 6th Annual Academy Awards

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Caspase Cleavage Sites in the Human Proteome: CaspDB, a Database of Predicted Substrates

PLOS ONE, Oct 2014

Caspases are enzymes belonging to a conserved family of cysteine-dependent aspartic-specific proteases that are involved in vital cellular processes and play a prominent role in apoptosis and inflammation. Determining all relevant protein substrates of caspases remains a challenging task. Over 1500 caspase substrates have been discovered in the human proteome according to published data and new substrates are discovered on a daily basis. To aid the discovery process we developed a caspase cleavage prediction method using the recently published curated MerCASBA database of experimentally determined caspase substrates and a Random Forest classification method. On both internal and external test sets, the ranking of predicted cleavage positions is superior to all previously developed prediction methods. The in silico predicted caspase cleavage positions in human proteins are available from a relational database: CaspDB. Our database provides information about potential cleavage sites in a verified set of all human proteins collected in Uniprot and their orthologs, allowing for tracing of cleavage motif conservation. It also provides information about the positions of disease-annotated single nucleotide polymorphisms, and posttranslational modifications that may modulate the caspase cleaving efficiency.

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Caspase Cleavage Sites in the Human Proteome: CaspDB, a Database of Predicted Substrates

a Database of Predicted Substrates. PLoS ONE 9(10): e110539. doi:10.1371/journal.pone.0110539 Caspase Cleavage Sites in the Human Proteome: CaspDB, a Database of Predicted Substrates Sonu Kumar 0 Bram J. van Raam 0 Guy S. Salvesen 0 Piotr Cieplak 0 Shawn B. Bratton, The University of Texas MD Anderson Cancer Center, United States of America 0 Sanford Burnham Medical Research Institute , La Jolla, California , United States of America Caspases are enzymes belonging to a conserved family of cysteine-dependent aspartic-specific proteases that are involved in vital cellular processes and play a prominent role in apoptosis and inflammation. Determining all relevant protein substrates of caspases remains a challenging task. Over 1500 caspase substrates have been discovered in the human proteome according to published data and new substrates are discovered on a daily basis. To aid the discovery process we developed a caspase cleavage prediction method using the recently published curated MerCASBA database of experimentally determined caspase substrates and a Random Forest classification method. On both internal and external test sets, the ranking of predicted cleavage positions is superior to all previously developed prediction methods. The in silico predicted caspase cleavage positions in human proteins are available from a relational database: CaspDB. Our database provides information about potential cleavage sites in a verified set of all human proteins collected in Uniprot and their orthologs, allowing for tracing of cleavage motif conservation. It also provides information about the positions of diseaseannotated single nucleotide polymorphisms, and posttranslational modifications that may modulate the caspase cleaving efficiency. - Data Availability: The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health (NIH) grants R01GM098835 (PC) and R01GM099040 (GSS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. Caspases are proteolytic enzymes that cleave a limited number of peptide bonds in proteins to regulate their function in diverse biological pathway(s). To date, 11 distinct caspases have been identified in humans with a similar number of homologs in other mammals [1]. They are involved in several functions such as the immune response, DNA replication, cell cycle progression, cell proliferation and apoptosis [2,3]. The most prominent feature of caspase-specificity is that caspases cleave their substrates almost exclusively after D residues. However, it has also been observed that a E residue at this position could sporadically replace D. Human caspases are divided into apoptotic (caspase-2, -3, -6, -7, -8, -9, and -10) and inflammatory (caspase-1, -4, and -5) members. The apoptotic members have been further sub-divided into initiators (caspase-2, -8, -9, and -10) and effectors (executioners) (caspase-3, -6, and -7). The initiator caspases have long pro domains containing a death-fold (death effector domain or caspase-recruitment domain (DED or CARD, respectively)) and require forced dimerization in a receptor complex for their activation, whereas the executioner caspases have short prodomains, exist as dimeric, inactive zymogens in the cytosol and require cleavage by an upstream protease (such as an initiator caspase) for their activation [4]. Based on the analysis of a number of cleavage site characteristics for apoptotic caspases it has been found that the caspase cleavage site has a general motif (DXXDA/G/S/T), pointing to the overlapping specificity of this family of enzymes [57]. Thus, caution is required when assigning a cleavage event to an individual caspase based on the cleavage motif alone. Besides the observed specificity for Asp residue at P1 there are other requirements before a peptide or protein can be considered a good substrate for a specific caspase [8]. For example, it has been observed that a small and uncharged residue (A, G, S, T and N) is preferred at the P1 position [9], while residues preferred at P4 are D for caspases-3/-7, I/L for caspases2/-8/-9/-10, or W/Y, V for caspases-1/-4/-5/-14, -6. At the P3 position all caspases prefer an E residue while no specific amino acid preference exists for the P2 position [10]. The binding site nomenclature is in accordance with Schechter and Berger [11]. In this study, we focus on the in silico prediction of human caspases substrates. During apoptosis, caspases initiate, coordinate and accelerate cell death and dismantling by cleaving crucial structural and enzymatic proteins. The cleavage efficiency depends on many factors including posttranslational modifications (PTMs) [12] and may be influenced by Single Nucleotide Polymorphisms (SNPs) that occur near the cleavage sites. Both effects may either increase [13] or decrease the cleavage efficiency, which means that they effectively regulate proteolysis. To understand the importance of the cleavage site motif and its regulation, one should carefully analyze the conservation of such cleavage sites in various organisms [14]. Hence, we designed a substrate prediction algorithm based on amino acid sequence specificity and predicted structural elements, and created CaspDB, a database of predicted caspase cleavage sites in human proteins. Our database integrates information about the cleavage positions with information about the conservation of cleavages in orthologous proteins, and available knowledge about the SNPs and PTMs. To date, several scoring functions or machine learning techniques have been implemented to predict caspase substrates. For example, GraBCas predicts potential caspase cleavage sites using position specific scoring [15]. PeptideCutter uses a limited experimental dataset to predict cleavage sites for a variety of proteases including some caspases [16]. PoPS provides a set of predefined protease specificity profiles or custom input cleavage site profiles, which are calculated based on the frequency of every amino acid at different positions in the vicinity of the cleavage site [17]. CAT3 is another tool based on scoring matrices, which predicts putative cleavage sites for caspase-3 [18]. CASVM [19] and PCSS [20] are support-vector-machine-based approaches that recognize putative cleavage sites of caspase substrates. Song et al. built Cascleave 1.0 using amino acid sequence, secondary structure, solvent accessibility and disorder region features as input to the support vector regression (SVR) models [21]. Recently, its second version, Cascleave 2.0 [22], has been developed for predicting potential cleavage sites for caspase-1,3,-6,-7,-8. The data for training their SVR model is obtained from the MEROPS [23] and Casbah database [24]. However, existing caspase cleavage prediction methods do not incorporate information about substrate conservation in other model organisms, or PTMs and SNPs that may influence cleavage efficiency. In addition, databases that list experimentally-derived putative caspase cleavage events using mass-spectrometry methods, such as Degrabase, are incomplete because of the low abundance or instability of certain substrates, the absence of other substrates from the cells used in the experiments or because tryptic digest of newly-cleaved proteins yields peptides that are either too small or too large to be accurately detected by MS/MS. For this study, we designed a novel caspase substrate prediction approach, which illuminates cleavage motif conservation and the influence of PTMs and SNPs. We tested several machine-learning classifiers and compared them with the selected available models. We selected the best model, a Random Forest classification method, and used it to predict potential caspase cleavage sites in all reviewed human proteins from the Uniprot portal [25]. A prediction model was constructed from an experimentally determined caspase cleavage dataset from the MerCASBA database [26], which is a curated version of the Casbah database [24]. All predicted cleavage sites were stored in a database named CaspDB. Due to overlapping cleavage motif selectivity of caspases and the lack of sufficient number of known substrates for some of the caspases, we avoided assigning the predicted cleavages to specific caspases. This assignment could be inferred a posteriori by comparing the predicted motif to the commonly observed cleavage sequence for each caspase, as described above. Obviously, our method produces many false-positive hits. This over-prediction is characteristic for all methods for proteolytic substrates prediction, since availability of the substrate and the influence of exosites cannot always be taken into account. However, our method consistently assigns high scores to the experimentally observed cleavage sites, and rank-orders them significantly better than previous methods. Thus, our database should be considered a useful tool to support and guide experimental exploration of pathways involving caspases. Results and Discussion Evaluation of the classifiers to predict cleavage sites Caspases are known to have a restricted substrate specificity for an aspartic acid residue at the P1 position while it is less restricted at P4-P2 and P1 positions in their target sequence. However, due to heterogeneity of the target sequences, the prediction of cleavage sites is a non-trivial task. Four classifiers (Table 1) were trained to predict caspase cleavage sites in protein sequences. Analysis of the training results allowed us to select the best classifier. Because for all classifiers the accuracy and AUC (area under the receiveroperating characteristics curve) have high values, they could not be the sole criteria for assessing classification models. For this purpose, we used several other characteristics including Costbenefit analysis values and Kappa statistical values [27], analogous to a correlation coefficient. Among the four classifiers, Random Forest was shown to yield highest kappa values, AUC, accuracy, and lowest cost for testing wrongly predicted cleavage sites. We therefore chose Random Forest as the most suitable model for predicting caspase cleavage sites. We applied this method to predict all potential caspase cleavage sites in an annotated set of more than 20,000 human proteins from Uniprot. The results of this prediction are provided by the CaspDB relational database. Description of CaspDB The CaspDB database is publically available at http://caspdb. for all users and no login or registering is required. It is a web-based, platform-independent database of predicted caspase cleavage sites in human proteins classified according to Uniprot. User input. For user convenience there are two query options to retrieve cleavage site information of substrates. The CaspDB can be accessed by either Uniprot id or Uniprot name. By default, the retrieved information is for cleavage sites containing aspartic acid at P1 position, but the user can also add predictions with glutamic acid at P1 position as an option. Another way to retrieve cleavage site information of substrates is by using a motif search. For example a motif DEVD-A/G/S/T has been observed to be preferred by caspase-3 in its target substrates. Using this motif search option all substrates containing a specified motif can be retrieved along with the prediction scores. Results. All the results are provided in a user-friendly tabular form. The result page contains information about: a) the P1 position of cleavage sites with score values (in the range 01), predicted secondary structure and disorder characteristics, and cleavage prediction class (yes or no), b) the presence of a signal peptide, c) graphical and tabular descriptions of the domain structure of queried proteins according to Pfam annotations [28], d) the list of PTMs and SNPs, including disease annotation of the latter, e) a multiple sequence alignment with available orthologs. The presence of signal peptide, as predicted by the SignalP v.4.0 program [29], indicates whether a protein is secreted. The domain annotation, which is included in the output page, is helpful in determining the inter- or intra-domain location of cleavage sites. All cleavage sites are arranged by default in descending order of score value. If a given substrate is experimentally annotated as a caspase substrate and reported in one of four known databases (MEROPS, Casbah, TopFIND 2.0 [30] and Degrabase [31]) then appropriate links to these databases are provided. To check conservations of a substrate cleavage sites in other organisms, orthologous proteins from 11 organisms are retrieved and shown in tabular form. There is a Compare button to perform pair-wise comparison of cleavage sites between a given substrate and its orthologous proteins. Results are shown as a ClustalW pairwise alignment along with cleavage sites tables for both the substrate and the ortholog, arranged next to each other in the form of look-up tables, which can be used for analysis of conservations of cleavage sites. Beneath this table, there is a button Start Jalview which can be used for displaying multiple sequence alignment of a substrate and all orthologous proteins. The output page also includes a list of SNPs and PTMs, with appropriate annotations, because both types of protein modification may affect caspase-mediated proteolysis. Case study and comparison To further demonstrate the predictive power of CaspDB, we evaluated a list of published known caspase substrates (Table 2), which were not included in the RF model learning set. The CaspDB RF model was able to recognize all cleavage sites correctly with a high probability score. We describe three examples from Table 2 that are highlighted in bold. The first example is the Pyrin protein (MEFV_HUMAN, Uniprot ID: O15553), which is involved in innate immunity and the inflammatory response [32]. It interacts with several components of the inflammasome complex through which it recruits and activates caspase-1, leading to cleavage at the D330 position: TCVRD-SCSFP. CaspDB correctly predicted this cleavage site with a cleavage probability score of 0.735. This cleavage site was also recognized by the latest web-server Cascleave 2.0, which gives a low probability score of 0.546, on a scale ranging from 0 to 1. The second example is Cytosolic phospholipase A2 (PA24A_HUMAN, Uniprot ID: P47712), which is involved in the inflammatory response [33]. It has one experimentally determined caspase8 cleavage site at D522: DDELD-AAVAD. CaspDB successfully predicted this cleavage site with a top score of 0.98, however Cascleave 2.0 scored this cleavage at 0.559. Another interesting example we evaluated with our model in which SNP (T300A) enhances caspase-3 cleavage efficiency is Autophagy-related protein 16-1 (A16L1_HUMAN, Uniprot ID: Q676U5) [13]. It has been observed that the T300A variant of A16L1 is sensitized to caspase-3-mediated cleavage, thereby revealing a functional connection between Crohns disease, caspase activation and autophagy. The experimentally known cleavage site of A16L1 is located at D299, within QDNVD-THPGS. For this protein our CaspDB model correctly predicts the cleavage site with a score of 0.965. We recalculated the score taking into account the SNP that leads to mutation T300A. This mutation increased the CaspDB score to 0.991 (QDNVD-AHPGS), in agreement with the results published by Murthy et al. [13] who observed that this SNP increases caspase cleavage efficiency. These results suggest that the CaspDB database can be used as useful tool for in silico cleavage site prediction. Inspection of Table 2 reveals that all of the cleavage sites are correctly and consistently scored highly by CaspDB. We also compared the predictive power of our CaspDB with Cascleave 2.0 using larger datasets. For this, we took known caspase-1 (114 cleavage sites from 100 substrates) and caspase-8 (57 cleavage sites from 38 substrates) substrates from the MEROPS database and calculated their cleavage site probability scores by both Cascleave 2.0 and CaspDB models. We found out that Cascleave 2.0 was able to score only 14 known cleavage sites of caspase-1 while CaspDB scores 111 out of 114 substrates above a threshold value of 0.5 (Figure 1A). For known cleavage sites of caspase-8, both Cascleave 2.0 and CaspDB predict cleavage positions correctly (Figure 1B). However, the CaspDB scores were higher than those obtained with Cascleave 2.0 in almost every case. Cascleave2.0 score Comparison of Cascleave 2.0 and CaspDB scores. doi:10.1371/journal.pone.0110539.t002 SNPs and PTMs analysis We performed an analysis on 520 experimentally known caspase substrates from the Casbah database for SNPs and PTMs in the vicinity of the scissile bond including P5-P4 positions. We found 28 unique caspase substrates with SNPs in the cleavage motif. Among them, 10 substrates have SNPs that are known to be associated with disease. We found 198 unique caspase substrates, out of 520, with PTMs sites in the vicinity of cleavage site (Table S1, S2). These SNPs and PTMs may affect the efficiency of caspase cleavage efficiency. However, we shall utilize this list as a basis for future research. In summary, we provide a user-friendly database for retrieving information about potential caspase cleavage sites for all human proteins classified as verified according to Uniprot annotation. Our caspase cleavage prediction model works better in comparison to other available methods. As opposed to other databases and prediction tools, the CaspDB provides additional information that would be indispensable in the generation of new hypotheses and in the verification of new experimental findings concerning caspase-mediated cleavage of putative substrates. Information about cleavage in orthologous proteins is useful in assessing conservation of the cleavage positions across species, and thus in assessing the confidence of the prediction. Integration of SNPs and PTMs information is very useful, since one can inspect how these effects may alter the proteolytic event. Overall, our database will complement on-going experimental efforts in identifying new caspase substrates and further our understanding of the biochemistry of caspase-mediated substrate cleavage. This knowledge will be helpful for resolving the larger role of these proteases and their targets in critical processes, such as apoptosis, necroptosis and inflammation. As more information about caspases and their substrates becomes available, we will update and improve the performance of our methodology. Materials and Methods Datasets We collected and curated substrate datasets for human caspases from the Casbah and MerCASBA databases. All the annotated substrate cleavage sites in the datasets were experimentally determined and thus not all putative cleavage sites were considered. The Casbah database contains 520 unique human proteins that are caspase substrates with a total of 661 experimentally determined cleavage sites (Table S3). Our training dataset was constructed from this positive dataset of 661 known cleavage sites and a negative (non-cleavage sites) dataset. The negative set was constructed in the following way: we used a sliding window approach and took two upstream (P3 and P2) and two downstream (P1 and P2) residues from experimentally verified cleavage sequences, and tentatively assigned them to the P1 position. Thus, we have 661 and 2637 sequences in the positive and negative datasets (Table S3), respectively, in a 1:4 ratio, as required by machine learning methods to accurately train classifiers. All the sequences within the training set were unique. The predicted models were built using several classifiers, as outlined below. Training of the classifiers We tested several classifiers from the Weka machine learning software suit [27] for training our dataset for predicting putative caspase cleavage sites in human proteins. We chose to test four different classifiers: Random Forest (RF), Nave Bayes (NB), decision tree algorithm (J48), and Sequential Minimal Optimization (SMO). We randomly divided our total positive and negative datasets into training (70%) and test (30%) datasets. The test datasets were used for evaluating the performance of each classifier model. In our prediction model we combined information related to amino acid sequence and the predicted structural features, including secondary structure and disorder, into input for the Weka program. In 2009 Timmer et al. already recognized the importance of these structural features in caspase substrate [34]. Information about the amino acid preferences at every P5-P3 position was defined in the form of the position weight matrix (PWM), which was calculated from known cleavage motifs in caspase substrates. The PWM has been calculated as follows. First, all sequences were aligned along the cleavage site and the frequency of occurrence of each amino acid in every P5-P3 position was calculated. Next, the frequency of occurrence of each amino acid at every position was normalized by the distribution of amino acids in the set of background sequences, as defined in the MerCASBA database. Thus, the PWM value for each amino acid iAA at the jth position was calculated as: PWM(iAA, j)~ Pbckgr(iAA, j) We used log2 values of each PWM (iAA, j) element of P5-P3 position for further calculations. The input into the Weka program is composed of individual records (lines) for each positive and negative sequence from the dataset. For each sequence the input consists of appropriate values of the PWMs, for every amino acid at each position, along with, description of the secondary structure (a-helix: H, b-sheet: E, loop: _) and disorder (ordered: . or disordered: *) for each residues at every P5-P3 position. The secondary structure assignments and disorder regions were predicted using Jnet [35] and DISOPRED2 [36], respectively. Classifier evaluation Each chosen classifier parameter (Table 3) was validated using a 10-fold cross validation. The number of true positives, false positives, true negatives and false negatives was counted and then the values of accuracy, precision, specificity, and Matthews Correlation Coefficient (MCC) and AUC (area under the receiver-operating characteristics (ROC) curve) parameters were calculated (Table 1). We also performed cost-benefit analysis using Weka, which determined the mean price/cost one should pay for discarding a very useful cleavage site and losing profit because of the wrong prediction taken by the classification model. Finally, all of these values were used for classifier evaluation. After classifier evaluation, we chose the best classifier for our cleavage prediction and then we bundled the test data back into training data to produce a set of input data to train the optimal select classifier for the actual prediction of caspase substrates in the entire human proteome (Table 1: RF_combined_set). Prediction of caspase cleavage sites in human proteome We downloaded all reviewed human proteins (20,266) from the Uniprot portal and then used CD-HIT [37] for eliminating redundant sequences. We used our best classifier model, Random Forest to predict the potential caspase cleavage sites in all human proteins. For comparison of conservation of cleavage sites in other organisms, we extracted the protein sequences from the proteomes of additional 11 organism that are orthologous to human proteins (Table S4) from the OMG browser [38]. This retrieval of orthologous proteins was based on Uniprot id mapping. The information about experimentally known PTMs and SNPs in each human protein was obtained from curated dbPTM [39] and Humsavar [40,41] databases, respectively. We also located the Random Forest (RF) Nave Bayes (NB) Number of Trees Number of folds Build logistic models Normalize training data positions of cleavage sites with respect to protein domains according to information retrieved from the Pfam database [28]. There are no restrictions to use and access of CaspDB, which is publicly available at Web implementation All predicted caspase cleavage sites in human proteins, mapped to their orthologous proteins, are stored online in the CaspDB database, available at: CaspDB is currently configured on an Apache (CentOS) server hosted at the Sanford Burnham Medical Research Institute (SBMRI) with the application program Hypertext Preprocessor (PHP). It has been developed based on a combination of three layers. The underlying layer is the MySQL database system, a relational database management system that stores all the information on the putative cleavage sites of human proteins and their orthologs along with the Pfam domains, SNPs and PTMs in the back-end and provides the facility to link two or more tables in the database. The intermediate layer is an Apache-PHP application that receives the query from the user and connects to the database to fetch data from the upper layer, which comprises populated HTML and PHP pages, to the web browser client. The PHP and Java scripts are embedded in the HTML web pages and are used as application programs for integrating the back-end (MySQL database) with the web pages (HTML). Apache is used as the web server for building the interface between the web browser and the application programs. HTML and PHP have been used to build the web interface. ClustalW [42] and Jalview [43] were implemented to show the pairwise alignment and multiple sequence alignment, respectively. Supporting Information Table S1 List of caspase substrates from Casbah database with SNPs in the vicinity of cleavage site. (XLS) Table S2 List of caspase substrates from Casbah database with PTMs in the vicinity of cleavage site. (XLSX) List of training datasets. List of proteomes and their orthologs. Acknowledgments The authors would like to thank David Huhta from Sanford-Burnham Medical Research Institute for his help in solving technical problems associated with setting up the CaspDB database. Conceived and designed the experiments: SK BJvR PC. Performed the experiments: SK PC. Analyzed the data: SK BJvR GSS PC. Contributed reagents/materials/analysis tools: SK PC. Contributed to the writing of the manuscript: SK BJvR GSS PC. Designed the software used in analysis: SK BJvR PC. 1. Chowdhury I , Tharakan B , Bhat GK ( 2008 ) Caspases - an update . Comp Biochem Physiol B Biochem Mol Biol 151 : 10 - 27 . 2. Dix MM , Simon GM , Cravatt BF ( 2008 ) Global mapping of the topography and magnitude of proteolytic events in apoptosis . Cell 134 : 679 - 691 . 3. Los M , Stroh C , Janicke RU , Engels IH , Schulze-Osthoff K ( 2001 ) Caspases: more than just killers? Trends Immunol 22 : 31 - 34 . 4. Pop C , Salvesen GS ( 2009 ) Human caspases: activation, specificity, and regulation . 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Sonu Kumar, Bram J. van Raam, Guy S. Salvesen, Piotr Cieplak. Caspase Cleavage Sites in the Human Proteome: CaspDB, a Database of Predicted Substrates, PLOS ONE, 2014, DOI: 10.1371/journal.pone.0110539


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